Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8MHY7 (UROK_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urokinase-type plasminogen activator

Short name=U-plasminogen activator
Short name=uPA
EC=3.4.21.73
Gene names
Name:PLAU
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation

Inhibited by SERPINA5 By similarity.

Subunit structure

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Subcellular location

Secreted By similarity.

Post-translational modification

Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 EGF-like domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 433417Urokinase-type plasminogen activator
PRO_0000285901
Chain21 – 179159Urokinase-type plasminogen activator long chain A By similarity
PRO_0000285902
Chain157 – 17923Urokinase-type plasminogen activator short chain A By similarity
PRO_0000285903
Chain181 – 433253Urokinase-type plasminogen activator chain B By similarity
PRO_0000285904

Regions

Domain29 – 6537EGF-like
Domain71 – 15383Kringle
Domain181 – 426246Peptidase S1
Region36 – 5924Binds urokinase plasminogen activator surface receptor By similarity
Region154 – 18027Connecting peptide By similarity

Sites

Active site2261Charge relay system By similarity
Active site2771Charge relay system By similarity
Active site3781Charge relay system By similarity

Amino acid modifications

Modified residue3251Phosphoserine By similarity
Glycosylation3241N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 41 By similarity
Disulfide bond35 ↔ 53 By similarity
Disulfide bond55 ↔ 64 By similarity
Disulfide bond72 ↔ 153 By similarity
Disulfide bond93 ↔ 135 By similarity
Disulfide bond124 ↔ 148 By similarity
Disulfide bond170 ↔ 301Interchain (between A and B chains) By similarity
Disulfide bond211 ↔ 227 By similarity
Disulfide bond219 ↔ 290 By similarity
Disulfide bond315 ↔ 384 By similarity
Disulfide bond347 ↔ 363 By similarity
Disulfide bond374 ↔ 402 By similarity

Experimental info

Sequence conflict2681R → S in AAM83187. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8MHY7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 6DD35A371010A6EE

FASTA43348,444
        10         20         30         40         50         60 
MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI WRCNCPKKFQ 

        70         80         90        100        110        120 
GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN VLTKTYHAHR PDALQLGLGK 

       130        140        150        160        170        180 
HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV HDCSSGKKPA LPPGKLEFQC GQKALRPRFK 

       190        200        210        220        230        240 
IIGGEFTIIE NQPWFAAIYR RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY 

       250        260        270        280        290        300 
LGRSRLNSMT PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI 

       310        320        330        340        350        360 
CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP HYYGSEVTTK 

       370        380        390        400        410        420 
MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR GCALKNKPGV YTRVSRFLPW 

       430 
IRSHIGEENG LAL 

« Hide

References

[1]"Downregulation of urokinase-type and tissue-type plasminogen activators in a rabbit model of renal ischemia/reperfusion."
Sugiki M., Omura S., Yoshida E., Itoh H., Kataoka H., Maruyama M.
J. Biochem. 132:501-508(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Increased expression of urokinase during atherosclerotic lesion development causes arterial constriction and lumen loss, and accelerates lesion growth."
Falkenberg M., Tom C., DeYoung M.B., Wen S., Linnemann R., Dichek D.A.
Proc. Natl. Acad. Sci. U.S.A. 99:10665-10670(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Oryctolagus cuniculus urokinase-type plasminogen activator, mRNA, complete cds."
Yano W., Watanabe M.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Rabbit urokinase plasminogen activator (genomic DNA)."
Graf S.K., Wen S., Dichek D.A.
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY029517 mRNA. Translation: AAK40239.1.
AY122285 mRNA. Translation: AAM83187.1.
AB087224 mRNA. Translation: BAC02685.1.
AY453856 Genomic DNA. Translation: AAR21232.1.
RefSeqNP_001075480.1. NM_001082011.1.
UniGeneOcu.2313.

3D structure databases

ProteinModelPortalQ8MHY7.
SMRQ8MHY7. Positions 31-157, 168-428.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.231.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155.
GeneID100008633.

Organism-specific databases

CTD5328.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115235.
HOGENOMHOG000237314.
HOVERGENHBG008633.
OMAKMLCAAD.
TreeFamTF329901.

Family and domain databases

Gene3D2.40.20.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUROK_RABIT
AccessionPrimary (citable) accession number: Q8MHY7
Secondary accession number(s): Q8MIL0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries