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Q8MHY7

- UROK_RABIT

UniProt

Q8MHY7 - UROK_RABIT

Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

    Catalytic activityi

    Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

    Enzyme regulationi

    Inhibited by SERPINA5.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei226 – 2261Charge relay systemBy similarity
    Active sitei277 – 2771Charge relay systemBy similarity
    Active sitei378 – 3781Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. fibrinolysis Source: Ensembl
    2. regulation of cell proliferation Source: Ensembl
    3. response to hypoxia Source: Ensembl
    4. smooth muscle cell migration Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Plasminogen activation

    Protein family/group databases

    MEROPSiS01.231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urokinase-type plasminogen activator (EC:3.4.21.73)
    Short name:
    U-plasminogen activator
    Short name:
    uPA
    Cleaved into the following 3 chains:
    Gene namesi
    Name:PLAU
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Chromosome 18

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 433417Urokinase-type plasminogen activatorPRO_0000285901Add
    BLAST
    Chaini21 – 179159Urokinase-type plasminogen activator long chain ABy similarityPRO_0000285902Add
    BLAST
    Chaini157 – 17923Urokinase-type plasminogen activator short chain ABy similarityPRO_0000285903Add
    BLAST
    Chaini181 – 433253Urokinase-type plasminogen activator chain BBy similarityPRO_0000285904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 41By similarity
    Disulfide bondi35 ↔ 53By similarity
    Disulfide bondi55 ↔ 64By similarity
    Disulfide bondi72 ↔ 153By similarity
    Disulfide bondi93 ↔ 135By similarity
    Disulfide bondi124 ↔ 148By similarity
    Disulfide bondi170 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
    Disulfide bondi211 ↔ 227By similarity
    Disulfide bondi219 ↔ 290By similarity
    Disulfide bondi315 ↔ 384By similarity
    Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
    Modified residuei325 – 3251PhosphoserineBy similarity
    Disulfide bondi347 ↔ 363By similarity
    Disulfide bondi374 ↔ 402By similarity

    Post-translational modificationi

    Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

    Interactioni

    Subunit structurei

    Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8MHY7.
    SMRiQ8MHY7. Positions 31-157, 168-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 6537EGF-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini71 – 15383KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini181 – 426246Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 5924Binds urokinase plasminogen activator surface receptorBy similarityAdd
    BLAST
    Regioni154 – 18027Connecting peptideBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115235.
    HOGENOMiHOG000237314.
    HOVERGENiHBG008633.
    OMAiKMLCAAD.
    TreeFamiTF329901.

    Family and domain databases

    Gene3Di2.40.20.10. 1 hit.
    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00181. EGF. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8MHY7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI    50
    WRCNCPKKFQ GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN 100
    VLTKTYHAHR PDALQLGLGK HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV 150
    HDCSSGKKPA LPPGKLEFQC GQKALRPRFK IIGGEFTIIE NQPWFAAIYR 200
    RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY LGRSRLNSMT 250
    PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI 300
    CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP 350
    HYYGSEVTTK MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR 400
    GCALKNKPGV YTRVSRFLPW IRSHIGEENG LAL 433
    Length:433
    Mass (Da):48,444
    Last modified:October 1, 2002 - v1
    Checksum:i6DD35A371010A6EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti268 – 2681R → S in AAM83187. (PubMed:12149463)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY029517 mRNA. Translation: AAK40239.1.
    AY122285 mRNA. Translation: AAM83187.1.
    AB087224 mRNA. Translation: BAC02685.1.
    AY453856 Genomic DNA. Translation: AAR21232.1.
    RefSeqiNP_001075480.1. NM_001082011.1.
    UniGeneiOcu.2313.

    Genome annotation databases

    EnsembliENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155.
    GeneIDi100008633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY029517 mRNA. Translation: AAK40239.1 .
    AY122285 mRNA. Translation: AAM83187.1 .
    AB087224 mRNA. Translation: BAC02685.1 .
    AY453856 Genomic DNA. Translation: AAR21232.1 .
    RefSeqi NP_001075480.1. NM_001082011.1.
    UniGenei Ocu.2313.

    3D structure databases

    ProteinModelPortali Q8MHY7.
    SMRi Q8MHY7. Positions 31-157, 168-428.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.231.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOCUT00000029709 ; ENSOCUP00000023296 ; ENSOCUG00000003155 .
    GeneIDi 100008633.

    Organism-specific databases

    CTDi 5328.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115235.
    HOGENOMi HOG000237314.
    HOVERGENi HBG008633.
    OMAi KMLCAAD.
    TreeFami TF329901.

    Family and domain databases

    Gene3Di 2.40.20.10. 1 hit.
    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00181. EGF. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Downregulation of urokinase-type and tissue-type plasminogen activators in a rabbit model of renal ischemia/reperfusion."
      Sugiki M., Omura S., Yoshida E., Itoh H., Kataoka H., Maruyama M.
      J. Biochem. 132:501-508(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Increased expression of urokinase during atherosclerotic lesion development causes arterial constriction and lumen loss, and accelerates lesion growth."
      Falkenberg M., Tom C., DeYoung M.B., Wen S., Linnemann R., Dichek D.A.
      Proc. Natl. Acad. Sci. U.S.A. 99:10665-10670(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Oryctolagus cuniculus urokinase-type plasminogen activator, mRNA, complete cds."
      Yano W., Watanabe M.
      Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Rabbit urokinase plasminogen activator (genomic DNA)."
      Graf S.K., Wen S., Dichek D.A.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiUROK_RABIT
    AccessioniPrimary (citable) accession number: Q8MHY7
    Secondary accession number(s): Q8MIL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3