Urokinase-type plasminogen activator precursor - Oryctolagus cuniculus (Rabbit)

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Q8MHY7 (UROK_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Urokinase-type plasminogen activator
Short name=U-plasminogen activator
Short name=uPA
EC=3.4.21.73

Cleaved into the following 3 chains:

Urokinase-type plasminogen activator long chain A
Urokinase-type plasminogen activator short chain A
Urokinase-type plasminogen activator chain B

Gene names

Name:

PLAU

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Catalytic activity	Specific cleavage of Arg-\|-Val bond in plasminogen to form plasmin.
Enzyme regulation	Inhibited by SERPINA5 By similarity.
Subunit structure	Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.
Subcellular location	Secreted By similarity.
Post-translational modification	Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fibrinolysis Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell adhesion mediated by integrin Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from electronic annotation. Source: Compara regulation of receptor activity Inferred from electronic annotation. Source: Compara regulation of smooth muscle cell migration Inferred from electronic annotation. Source: Compara regulation of smooth muscle cell-matrix adhesion Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara smooth muscle cell migration Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

Potential

Chain

17 – 433

417

Urokinase-type plasminogen activator

PRO_0000285901

Chain

21 – 179

159

Urokinase-type plasminogen activator long chain A By similarity

PRO_0000285902

Chain

157 – 179

Urokinase-type plasminogen activator short chain A By similarity

PRO_0000285903

Chain

181 – 433

253

Urokinase-type plasminogen activator chain B By similarity

PRO_0000285904

Regions

Domain

29 – 65

EGF-like

Domain

71 – 153

Kringle

Domain

181 – 426

246

Peptidase S1

Region

36 – 59

Binds urokinase plasminogen activator surface receptor By similarity

Region

154 – 180

Connecting peptide By similarity

Sites

Active site

226

Charge relay system By similarity

Active site

277

Charge relay system By similarity

Active site

378

Charge relay system By similarity

Amino acid modifications

Modified residue

325

Phosphoserine By similarity

Glycosylation

324

N-linked (GlcNAc...) Potential

Disulfide bond

33 ↔ 41

By similarity

Disulfide bond

35 ↔ 53

By similarity

Disulfide bond

55 ↔ 64

By similarity

Disulfide bond

72 ↔ 153

By similarity

Disulfide bond

93 ↔ 135

By similarity

Disulfide bond

124 ↔ 148

By similarity

Disulfide bond

170 ↔ 301

Interchain (between A and B chains) By similarity

Disulfide bond

211 ↔ 227

By similarity

Disulfide bond

219 ↔ 290

By similarity

Disulfide bond

315 ↔ 384

By similarity

Disulfide bond

347 ↔ 363

By similarity

Disulfide bond

374 ↔ 402

By similarity

Experimental info

Sequence conflict

268

R → S in AAM83187. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q8MHY7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 6DD35A371010A6EE
Show »

FASTA

433

48,444

        10         20         30         40         50         60 
MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI WRCNCPKKFQ 

        70         80         90        100        110        120 
GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN VLTKTYHAHR PDALQLGLGK 

       130        140        150        160        170        180 
HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV HDCSSGKKPA LPPGKLEFQC GQKALRPRFK 

       190        200        210        220        230        240 
IIGGEFTIIE NQPWFAAIYR RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY 

       250        260        270        280        290        300 
LGRSRLNSMT PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI 

       310        320        330        340        350        360 
CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP HYYGSEVTTK 

       370        380        390        400        410        420 
MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR GCALKNKPGV YTRVSRFLPW 

       430 
IRSHIGEENG LAL

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References

[1]	"Downregulation of urokinase-type and tissue-type plasminogen activators in a rabbit model of renal ischemia/reperfusion." Sugiki M., Omura S., Yoshida E., Itoh H., Kataoka H., Maruyama M. J. Biochem. 132:501-508(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Increased expression of urokinase during atherosclerotic lesion development causes arterial constriction and lumen loss, and accelerates lesion growth." Falkenberg M., Tom C., DeYoung M.B., Wen S., Linnemann R., Dichek D.A. Proc. Natl. Acad. Sci. U.S.A. 99:10665-10670(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Oryctolagus cuniculus urokinase-type plasminogen activator, mRNA, complete cds." Yano W., Watanabe M. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Rabbit urokinase plasminogen activator (genomic DNA)." Graf S.K., Wen S., Dichek D.A. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY029517 mRNA. Translation: AAK40239.1. AY122285 mRNA. Translation: AAM83187.1. AB087224 mRNA. Translation: BAC02685.1. AY453856 Genomic DNA. Translation: AAR21232.1.
RefSeq	NP_001075480.1. NM_001082011.1.
UniGene	Ocu.2313.
3D structure databases
HSSP	HSSP built from PDB template 1LMW based on UniProtKB P00749.
ProteinModelPortal	Q8MHY7.
SMR	Q8MHY7. Positions 31-157, 168-428.
ModBase	Search...
Protein family/group databases
MEROPS	S01.231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155.
GeneID	100008633.
Organism-specific databases
CTD	5328.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00570000078945.
HOGENOM	HOG000237314.
HOVERGEN	HBG008633.
OMA	CQQPHYY.
OrthoDB	EOG4229K6.
Family and domain databases
Gene3D	2.40.20.10. 1 hit.
InterPro	IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00181. EGF. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF57440. Kringle-like. 1 hit. SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UROK_RABIT

Accession

Primary (citable) accession number: Q8MHY7
Secondary accession number(s): Q8MIL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 3 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents