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Q8LW54 (MEL_APICE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melittin
Gene names
Name:MELT
OrganismApis cerana (Indian honeybee)
Taxonomic identifier7461 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length77 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Main toxin of bee venom with strong hemolytic activity. Integrates into cell membranes and has multiple effects, probably, as a result of its interaction with negatively charged phospholipids. It inhibits well known transport pumps such as the Na+-K+-ATPase and the H+-K+-ATPase. Increases the permeability of cell membranes to ions, particularly Na+ and indirectly Ca2+, because of the Na+-Ca2+-exchange By similarity.

Subunit structure

Monomer and homotetramer By similarity.

Subcellular location

Secreted. Target cell membrane By similarity. Note: Forms a transmembrane alpha-helix in the target cell membrane. Forms a membrane channel in the prey By similarity.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the melittin family.

Ontologies

Keywords
   Biological processCytolysis
Hemolysis
Ion transport
Transport
   Cellular componentMembrane
Secreted
Target cell membrane
Target membrane
   DomainSignal
Transmembrane
   Molecular functionToxin
   PTMAmidation
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processhemolysis in other organism

Inferred from electronic annotation. Source: UniProtKB-KW

ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

other organism cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein kinase inhibitor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Propeptide31 – 5020Removed by a dipeptidylpeptidase By similarity
PRO_0000035146
Peptide51 – 7626Melittin Ref.2
PRO_0000035147

Amino acid modifications

Modified residue761Glutamine amide Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8LW54 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 091BEF7CE6019374

FASTA778,515
        10         20         30         40         50         60 
MKFLVNVALV FYGRVHFLHL CVHFLHLWAP EPEPAPEAEA EADAEADPEA GIGAVLKVLT 

        70 
TGLPALISWI KRKRQQG 

« Hide

References

[1]"Gene encoding melittin in honeybee Apis cerana colleted in Hanoi Vietnam."
Le H.Q., Le B.T.
Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure of melittin isolated from two species of honey bees."
Kreil G.
FEBS Lett. 33:241-244(1973)
Cited for: PROTEIN SEQUENCE OF 51-76, AMIDATION AT GLN-76.

Web resources

Protein Spotlight

Why Pooh luvvs hunny - Issue 12 of July 2001

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ489619 Genomic DNA. Translation: CAD33921.1.
PIRMEHBCI. A01762.

3D structure databases

ProteinModelPortalQ8LW54.
SMRQ8LW54. Positions 51-76.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002116. Melittin/Api_allergen.
[Graphical view]
PfamPF01372. Melittin. 1 hit.
[Graphical view]
ProDomPD014636. Melittin/Api_allergen. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameMEL_APICE
AccessionPrimary (citable) accession number: Q8LW54
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries