Q8LW54 (MEL_APICE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melittin

Gene names

Name:

MELT

Organism

Apis cerana (Indian honeybee)

Taxonomic identifier

7461 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis

Protein attributes

Sequence length	77 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Main toxin of bee venom with strong hemolytic activity. Integrates into cell membranes and has multiple effects, probably, as a result of its interaction with negatively charged phospholipids. It inhibits well known transport pumps such as the Na+-K+-ATPase and the H+-K+-ATPase. Increases the permeability of cell membranes to ions, particularly Na+ and indirectly Ca2+, because of the Na+-Ca2+-exchange By similarity.
Subunit structure	Monomer and homotetramer By similarity.
Subcellular location	Secreted. Target cell membrane By similarity. Note: Forms a transmembrane alpha-helix in the target cell membrane. Forms a membrane channel in the prey By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the melittin family.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis Ion transport Transport
Cellular component	Membrane Secreted Target cell membrane Target membrane
Domain	Signal Transmembrane
Molecular function	Toxin
PTM	Amidation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW ion transport Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of protein kinase activity Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW other organism cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein kinase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Propeptide	31 – 50	20	Removed by a dipeptidylpeptidase By similarity		PRO_0000035146
	Peptide	51 – 76	26	Melittin Ref.2		PRO_0000035147
Amino acid modifications
	Modified residue	76	1	Glutamine amide Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8LW54 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 091BEF7CE6019374	FASTA	77	8,515
	10 20 30 40 50 60 MKFLVNVALV FYGRVHFLHL CVHFLHLWAP EPEPAPEAEA EADAEADPEA GIGAVLKVLT 70 TGLPALISWI KRKRQQG

References

[1]	"Gene encoding melittin in honeybee Apis cerana colleted in Hanoi Vietnam." Le H.Q., Le B.T. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of melittin isolated from two species of honey bees." Kreil G. FEBS Lett. 33:241-244(1973) Cited for: PROTEIN SEQUENCE OF 51-76, AMIDATION AT GLN-76.

Web resources

Protein Spotlight

Why Pooh luvvs hunny - Issue 12 of July 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ489619 Genomic DNA. Translation: CAD33921.1.
PIR	MEHBCI. A01762.
3D structure databases
ProteinModelPortal	Q8LW54.
SMR	Q8LW54. Positions 51-76.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002116. Melittin/Api_allergen. [Graphical view]
Pfam	PF01372. Melittin. 1 hit. [Graphical view]
ProDom	PD014636. Melittin/Api_allergen. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MEL_APICE

Accession

Primary (citable) accession number: Q8LW54

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	October 1, 2002
Last modified:	October 3, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program