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Protein

Melittin

Gene

MELT

Organism
Apis cerana (Indian honeybee)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Main toxin of bee venom with strong hemolytic activity. Integrates into cell membranes and has multiple effects, probably, as a result of its interaction with negatively charged phospholipids. It inhibits well known transport pumps such as the Na+-K+-ATPase and the H+-K+-ATPase. Increases the permeability of cell membranes to ions, particularly Na+ and indirectly Ca2+, because of the Na+-Ca2+-exchange (By similarity).By similarity

GO - Molecular functioni

  1. protein kinase inhibitor activity Source: InterPro

GO - Biological processi

  1. hemolysis in other organism Source: UniProtKB-KW
  2. ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Cytolysis, Hemolysis, Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Melittin
Gene namesi
Name:MELT
OrganismiApis cerana (Indian honeybee)
Taxonomic identifieri7461 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Subcellular locationi

Secreted. Target cell membrane By similarity
Note: Forms a transmembrane alpha-helix in the target cell membrane. Forms a membrane channel in the prey (By similarity).By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. other organism cell membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted, Target cell membrane, Target membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Propeptidei31 – 5020Removed by a dipeptidylpeptidaseBy similarityPRO_0000035146Add
BLAST
Peptidei51 – 7626MelittinPRO_0000035147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei76 – 761Glutamine amide1 Publication

Keywords - PTMi

Amidation

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer and homotetramer.By similarity

Structurei

3D structure databases

SMRiQ8LW54. Positions 51-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the melittin family.Curated

Keywords - Domaini

Signal, Transmembrane

Family and domain databases

InterProiIPR002116. Melittin/Api_allergen.
[Graphical view]
PfamiPF01372. Melittin. 1 hit.
[Graphical view]
ProDomiPD014636. Melittin/Api_allergen. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LW54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLVNVALV FYGRVHFLHL CVHFLHLWAP EPEPAPEAEA EADAEADPEA
60 70
GIGAVLKVLT TGLPALISWI KRKRQQG
Length:77
Mass (Da):8,515
Last modified:October 1, 2002 - v1
Checksum:i091BEF7CE6019374
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ489619 Genomic DNA. Translation: CAD33921.1.
PIRiA01762. MEHBCI.

Cross-referencesi

Web resourcesi

Protein Spotlight

Why Pooh luvvs hunny - Issue 12 of July 2001

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ489619 Genomic DNA. Translation: CAD33921.1.
PIRiA01762. MEHBCI.

3D structure databases

SMRiQ8LW54. Positions 51-76.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002116. Melittin/Api_allergen.
[Graphical view]
PfamiPF01372. Melittin. 1 hit.
[Graphical view]
ProDomiPD014636. Melittin/Api_allergen. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Gene encoding melittin in honeybee Apis cerana colleted in Hanoi Vietnam."
    Le H.Q., Le B.T.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of melittin isolated from two species of honey bees."
    Kreil G.
    FEBS Lett. 33:241-244(1972)
    Cited for: PROTEIN SEQUENCE OF 51-76, AMIDATION AT GLN-76.

Entry informationi

Entry nameiMEL_APICE
AccessioniPrimary (citable) accession number: Q8LW54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: October 1, 2002
Last modified: April 1, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.