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Protein

E2F transcription factor-like E2FE

Gene

E2FE

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibitor of E2F-dependent activation of gene expression. Binds specifically the E2 recognition site without interacting with DP proteins and prevents transcription activation by E2F/DP heterodimers. Controls the timing of endocycle onset and inhibits endoreduplication.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi34 – 9966Add
BLAST
DNA bindingi169 – 25082Add
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • DNA endoreduplication Source: TAIR
  • negative regulation of DNA endoreduplication Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
E2F transcription factor-like E2FE
Alternative name(s):
DP-E2F-like protein 1
E2F-like repressor E2L3
Gene namesi
Name:E2FE
Synonyms:DEL1, E2L3, ELP2
Ordered Locus Names:At3g48160
ORF Names:T24C20.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G48160.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, but increased ploidy levels.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403E2F transcription factor-like E2FEPRO_0000406293Add
BLAST

Proteomic databases

PaxDbiQ8LSZ4.
PRIDEiQ8LSZ4.

PTM databases

iPTMnetiQ8LSZ4.

Expressioni

Tissue specificityi

Expressed exclusively in mitotically dividing cells. Highly expressed in young leaves and mature flowers. Lower expression in young stalk and in young and mature flowers.2 Publications

Developmental stagei

Expressed in a cell cycle-dependent manner. Not detected during early S phase. Expressed at both the G1/S and S/G2 transitions, with a peak during G2.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi9290. 11 interactions.
IntActiQ8LSZ4. 9 interactions.
STRINGi3702.AT3G48160.2.

Structurei

3D structure databases

ProteinModelPortaliQ8LSZ4.
SMRiQ8LSZ4. Positions 33-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi285 – 2917Poly-Ser

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2578. Eukaryota.
ENOG4111IGY. LUCA.
HOGENOMiHOG000083291.
InParanoidiQ8LSZ4.
OMAiMQGAGNI.
PhylomeDBiQ8LSZ4.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 2 hits.
PfamiPF02319. E2F_TDP. 2 hits.
[Graphical view]
SMARTiSM01372. E2F_TDP. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Named isoforms=2.

Isoform 1 (identifier: Q8LSZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDLSPERFK LAVTSPSSIP ESSSALQLHH SYSRKQKSLG LLCTNFLALY
60 70 80 90 100
NREGIEMVGL DDAASKLGVE RRRIYDIVNV LESVGVLTRR AKNQYTWKGF
110 120 130 140 150
SAIPGALKEL QEEGVKDTFH RFYVNENVKG SDDEDDDEES SQPHSSSQTD
160 170 180 190 200
SSKPGSLPQS SDPSKIDNRR EKSLGLLTQN FIKLFICSEA IRIISLDDAA
210 220 230 240 250
KLLLGDAHNT SIMRTKVRRL YDIANVLSSM NLIEKTHTLD SRKPAFKWLG
260 270 280 290 300
YNGEPTFTLS SDLLQLESRK RAFGTDITNV NVKRSKSSSS SQENATERRL
310 320 330 340 350
KMKKHSTPES SYNKSFDVHE SRHGSRGGYH FGPFAPGTGT YPTAGLEDNS
360 370 380 390 400
RRAFDVENLD SDYRPSYQNQ VLKDLFSHYM DAWKTWFSEV TQENPLPNTS

QHR
Length:403
Mass (Da):45,461
Last modified:October 1, 2002 - v1
Checksum:i0C9E724B5AF7BC94
GO
Isoform 2 (identifier: Q8LSZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     371-379: VLKDLFSHY → GAYILFTSI
     380-403: Missing.

Show »
Length:379
Mass (Da):42,438
Checksum:i6F0BD42C6DDA7F76
GO

Sequence cautioni

The sequence CAB51063.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei371 – 3799VLKDLFSHY → GAYILFTSI in isoform 2. 1 PublicationVSP_040805
Alternative sequencei380 – 40324Missing in isoform 2. 1 PublicationVSP_040806Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074533 mRNA. Translation: BAB91414.1.
AJ417836 mRNA. Translation: CAD10633.1.
AL096856 Genomic DNA. Translation: CAB51063.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE78374.1.
CP002686 Genomic DNA. Translation: AEE78375.1.
BT004258 mRNA. Translation: AAO42262.1.
BT005524 mRNA. Translation: AAO63944.1.
PIRiT13005.
RefSeqiNP_190399.2. NM_114685.3. [Q8LSZ4-1]
NP_851012.1. NM_180681.1. [Q8LSZ4-2]
UniGeneiAt.35736.

Genome annotation databases

EnsemblPlantsiAT3G48160.2; AT3G48160.2; AT3G48160. [Q8LSZ4-1]
GeneIDi823971.
KEGGiath:AT3G48160.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074533 mRNA. Translation: BAB91414.1.
AJ417836 mRNA. Translation: CAD10633.1.
AL096856 Genomic DNA. Translation: CAB51063.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE78374.1.
CP002686 Genomic DNA. Translation: AEE78375.1.
BT004258 mRNA. Translation: AAO42262.1.
BT005524 mRNA. Translation: AAO63944.1.
PIRiT13005.
RefSeqiNP_190399.2. NM_114685.3. [Q8LSZ4-1]
NP_851012.1. NM_180681.1. [Q8LSZ4-2]
UniGeneiAt.35736.

3D structure databases

ProteinModelPortaliQ8LSZ4.
SMRiQ8LSZ4. Positions 33-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9290. 11 interactions.
IntActiQ8LSZ4. 9 interactions.
STRINGi3702.AT3G48160.2.

PTM databases

iPTMnetiQ8LSZ4.

Proteomic databases

PaxDbiQ8LSZ4.
PRIDEiQ8LSZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G48160.2; AT3G48160.2; AT3G48160. [Q8LSZ4-1]
GeneIDi823971.
KEGGiath:AT3G48160.

Organism-specific databases

TAIRiAT3G48160.

Phylogenomic databases

eggNOGiKOG2578. Eukaryota.
ENOG4111IGY. LUCA.
HOGENOMiHOG000083291.
InParanoidiQ8LSZ4.
OMAiMQGAGNI.
PhylomeDBiQ8LSZ4.

Miscellaneous databases

PROiQ8LSZ4.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 2 hits.
PfamiPF02319. E2F_TDP. 2 hits.
[Graphical view]
SMARTiSM01372. E2F_TDP. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The E2F family of transcription factors from Arabidopsis thaliana. Novel and conserved components of the retinoblastoma/E2F pathway in plants."
    Mariconti L., Pellegrini B., Cantoni R., Stevens R., Bergounioux C., Cella R., Albani D.
    J. Biol. Chem. 277:9911-9919(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DEVELOPMENTAL STAGE, GENE FAMILY, NOMENCLATURE.
  2. "E2Ls, E2F-like repressors of Arabidopsis that bind to E2F sites in a monomeric form."
    Kosugi S., Ohashi Y.
    J. Biol. Chem. 277:16553-16558(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
    Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
    Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiE2FE_ARATH
AccessioniPrimary (citable) accession number: Q8LSZ4
Secondary accession number(s): Q8RYD8, Q9STS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.