Reviewed, UniProtKB/Swiss-Prot Q8LQ92 (GUN3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 3
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 3
    OsGLU8

Gene names

Name:	GLU8
Ordered Locus Names:	Os01g0312800, LOC_Os01g21070
ORF Names:	B1011A07.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in flowers. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 499	480	Endoglucanase 3		PRO_0000249280
Sites
	Active site	416	1	By similarity
	Active site	467	1	By similarity
	Active site	476	1	By similarity
Experimental info
	Sequence conflict	94	1	F → S in AK106887. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LQ92-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 73B3C65F02005791	FASTA	499	54,427
	10 20 30 40 50 60 MALLRCLFLL AVLLPHRNAA VVAAASPHHG PAPHDYRDAL TKSILFFEGQ RSGKLPPSQR 70 80 90 100 110 120 VSWRGDSGLS DGSSIKVDLV GGYYDAGDNM KFGFPLAFSM TMLAWSVVEF GGLMKGELQH 130 140 150 160 170 180 ARDAVRWGSD YLLKATAHPD TVYVQVGDAN RDHACWERPE DMDTPRTVYK VDPSTPGTDV 190 200 210 220 230 240 AAETAAALAA ASLVFRKSDP AYASRLVARA KRVFEFADKH RGTYSTRLSP YVCPYYCSYS 250 260 270 280 290 300 GYQDELLWGA AWLHRATKNP TYLSYIQMNG QVLGADEQDN TFGWDNKHAG ARILIAKAFL 310 320 330 340 350 360 VQKVAALHEY KGHADSFICS MVPGTPTDQT QYTRGGLLFK LSDSNMQYVT SSSFLLLTYA 370 380 390 400 410 420 KYLAFSKTTV SCGGAAVTPA RLRAIARQQV DYLLGSNPMG MSYMVGYGAK YPRRIHHRAS 430 440 450 460 470 480 SLPSVAAHPA RIGCSQGFTA LYSGVANPNV LVGAVVGGPN LQDQFPDQRS DHEHSEPATY 490 INAPLVGALA YLAHSYGQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003722 Genomic DNA. Translation: BAB92772.1. AP008207 Genomic DNA. Translation: BAF04781.1. Different initiation. AK106887 mRNA. No translation available.
RefSeq	NP_001042867.1.
UniGene	Os.28032
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
SMR	Q8LQ92. Positions 33-498.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4327286.
KEGG	osa:4327286.
NMPDR	fig|39947.1.peg.218.
Organism-specific databases
Gramene	Q8LQ92.
Phylogenomic databases
HOGENOM	HBG746347.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN3_ORYSJ

Accession

Primary (citable) accession number: Q8LQ92
Secondary accession number(s): Q0JN97

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)