Reviewed, UniProtKB/Swiss-Prot Q8LQ68 (HXK6_ORYSJ)

Names and origin

Protein names

Recommended name:
    Hexokinase-6
    EC=2.7.1.1
Alternative name(s):
    Hexokinase-2

Gene names

Name:	HXK6
Synonyms:	HXK2
Ordered Locus Names:	Os01g0742500, LOC_Os01g53930
ORF Names:	P0439E07.19

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from plastids to cytosol By similarity. Ref.1
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Plastid › chloroplast outer membrane; Single-pass membrane protein By similarity.
Tissue specificity	Expressed in roots, leaves, flowers, immature seeds and endosperm. Ref.1
Developmental stage	Expressed during flower development until 15 days after flowering. Ref.1
Induction	By glucose or fructose treatment in leaves. Ref.1
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Membrane Plastid Plastid outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Hexokinase-6		PRO_0000247569
Regions
	Transmembrane	6 – 26	21	Potential
	Nucleotide binding	109 – 114	6	ATP Potential
	Region	179 – 205	27	Glucose-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8LQ68-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 242F8DCCB5FF8947	FASTA	506	55,121
	10 20 30 40 50 60 MGKGTVVGTA VVVCAAAAAA VGVAVVVSRR RRSKREAEEE RRRRAAAVIE EVEQRFSTPT 70 80 90 100 110 120 ALLRGIADAM VEEMERGLRA DPHAPLKMLI SYVDNLPTGD EHGLFYALDL GGTNFRVIRV 130 140 150 160 170 180 QLGGREKRVV SQQYEEVAIP PHLMVGTSME LFDFIAAELE SFVKTEGEDF HLPEGRQREL 190 200 210 220 230 240 GFTFSFPVHQ TSISSGTLIK WTKGFSINGT VGEDVVAELS RAMERQGLDM KVTALVNDTV 250 260 270 280 290 300 GTLAGGRYVD NDVAAAVILG TGTNAAYVEH ANAIPKWTGL LPRSGNMVIN MEWGNFKSER 310 320 330 340 350 360 LPRSDYDNAL DFESLNPGEQ IYEKMISGMY LGEIVRRILL KLAHDASLFG DVVPTKLEQR 370 380 390 400 410 420 FILRTPDMSA MHHDTSHDLK HLGAKLKDIL GVADTSLEAR YITLHVCDLV AERGARLAAA 430 440 450 460 470 480 GIYGILKKLG RDRVPSDGSQ KQRTVIALDG GLYEHYKKFR TCLEATLADL LGEEAASSVV 490 500 VKLANDGSGI GAALLAASHS QYASVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.)." Cho J.-I., Ryoo N., Ko S., Lee S.-K., Lee J., Jung K.-H., Lee Y.-H., Bhoo S.H., Winderickx J., An G., Hahn T.-R., Jeon J.-S. Planta 224:598-611(2006) [PubMed: 16552590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, NOMENCLATURE. Strain: cv. Jinmi.
[2]	"The hexokinase gene family in rice." Wang Y.D., Cheng W., Wang X.S., Zhou X.J. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Zhonghua 15. Tissue: Flower.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116388 mRNA. Translation: AAZ93623.1. AY884165 mRNA. Translation: AAX68418.1. AP003768 Genomic DNA. Translation: BAB91930.1.
RefSeq	NP_001044214.1.
UniGene	Os.5547
3D structure databases
SMR	Q8LQ68. Positions 49-501.
ModBase	Search...
Genome annotation databases
GeneID	4326547.
KEGG	osa:4326547.
NMPDR	fig|39947.1.peg.8111.
Organism-specific databases
Gramene	Q8LQ68.
Phylogenomic databases
PhylomeDB	Q8LQ68.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. IPR001092. HLH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK6_ORYSJ

Accession

Primary (citable) accession number: Q8LQ68

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)