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Protein

Histone acetyltransferase type B catalytic subunit

Gene

HAT1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acetylates newly synthesized histones during DNA replication. Highly specific in vitro for the non-acetylated H4 which is acetylated sequentially at 'Lys-12' and 'Lys-5' into a di-acetylated form.

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Enzyme regulationi

Inhibited by 5 mM of zinc, copper and iron. Unaffected by low concentrations of detergents and irreversibly inactivated by 2% ethanol, isopropanol or dimethyl sulfoxide.

Kineticsi

  1. KM=25 µM for core histones1 Publication
  2. KM=9 µM for acetyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.2-8.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei276Proton donor/acceptorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.3.1.48 6752

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone acetyltransferase type B catalytic subunit (EC:2.3.1.48By similarity)
    Alternative name(s):
    Histone acetyltransferase HAT B
    Histone acetyltransferase HAT-B-p50
    Gene namesi
    Name:HAT1
    Synonyms:HAC106, HATB1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
    Proteomesi
    • UP000007305 Componenti: Unplaced

    Organism-specific databases

    MaizeGDBi273678

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002321261 – 468Histone acetyltransferase type B catalytic subunitAdd BLAST468

    Proteomic databases

    PaxDbiQ8LPU4
    PRIDEiQ8LPU4

    Expressioni

    Developmental stagei

    Found in the dry embryo (at protein level). Maximum level of expression 22 hours after start of seed imbibition.1 Publication

    Gene expression databases

    ExpressionAtlasiQ8LPU4 differential

    Interactioni

    Subunit structurei

    Heteromer of HAT1/p50 and p45 subunits.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei200Interaction with histone H4 N-terminusBy similarity1

    Protein-protein interaction databases

    STRINGi4577.GRMZM5G851405_P02

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LPU4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni242 – 244Acetyl-CoA bindingBy similarity3
    Regioni249 – 255Acetyl-CoA bindingBy similarity7

    Sequence similaritiesi

    Belongs to the HAT1 family.Curated

    Phylogenomic databases

    eggNOGiKOG2696 Eukaryota
    ENOG410XPH4 LUCA
    HOGENOMiHOG000084749
    KOiK11303

    Family and domain databases

    Gene3Di3.90.360.10, 2 hits
    InterProiView protein in InterPro
    IPR016181 Acyl_CoA_acyltransferase
    IPR019467 Hat1_N
    IPR037113 Hat1_N_sf
    IPR017380 Hist_AcTrfase_B-typ_cat-su
    PANTHERiPTHR12046 PTHR12046, 1 hit
    PfamiView protein in Pfam
    PF10394 Hat1_N, 1 hit
    PIRSFiPIRSF038084 HAT-B_cat, 1 hit
    SUPFAMiSSF55729 SSF55729, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8LPU4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALKQKDTDA AATATGTKKR RRVFFSDTDA GVEANECMKV FLVWNPGEVS
    60 70 80 90 100
    SVDCTAIQPF DLNHFFGEDG KIYGYKNLKI NVWISAKSFH GYADVSFDET
    110 120 130 140 150
    SDGGKGITDL KPVLQNIFGE NLVEKEEFLH TFSKECEYIR TAVTNGSAIK
    160 170 180 190 200
    HDGSYESDPA VEIVRVELQG AAAFLYSRLV PLVLLLVEGS TPIDIGEHGW
    210 220 230 240 250
    EMLLVVKKAT QEAGSKFELL GFAAVHNFYH YPESIRLRIS QILVLPPYQG
    260 270 280 290 300
    EGHGLGLLEA INYIAQSENI YDVTIESPSD YLQYVRSSID CLRLLMFDPI
    310 320 330 340 350
    KPALGAIVLS LKETNLSKRA QSLRMVPPAD LMETVRQKLK INKKQFLRCW
    360 370 380 390 400
    EILVFLSLDS QDHKSMDNFR ACIYDRMKGE ILGSASGTNR KRLLQMPTSF
    410 420 430 440 450
    NKEASFAVYW TQEIEDEDEQ TVEQQPEDLK TQEQQLNELV DIQIEEIAGV
    460
    AKNVTSRCKD KMTELVVQ
    Length:468
    Mass (Da):52,721
    Last modified:April 4, 2006 - v2
    Checksum:i049F68157ED8443C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti354V → I in AAM28228 (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U90274 mRNA Translation: AAC03423.2
    AF171927 Genomic DNA Translation: AAF06742.1
    AY093417 mRNA Translation: AAM28228.1
    PIRiT02064
    RefSeqiNP_001105187.1, NM_001111717.1
    XP_008651846.1, XM_008653624.1
    UniGeneiZm.94647

    Genome annotation databases

    GeneIDi542083
    KEGGizma:542083

    Similar proteinsi

    Entry informationi

    Entry nameiHAT1_MAIZE
    AccessioniPrimary (citable) accession number: Q8LPU4
    Secondary accession number(s): O49994
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: April 4, 2006
    Last modified: May 23, 2018
    This is version 80 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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