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Q8LPU4

- HAT1_MAIZE

UniProt

Q8LPU4 - HAT1_MAIZE

Protein

Histone acetyltransferase type B catalytic subunit

Gene

HAT1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (04 Apr 2006)
      Previous versions | rss
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    Functioni

    Acetylates newly synthesized histones during DNA replication. Highly specific in vitro for the non-acetylated H4 which is acetylated sequentially at 'Lys-12' and 'Lys-5' into a di-acetylated form.

    Catalytic activityi

    Acetyl-CoA + [histone] = CoA + acetyl-[histone].

    Enzyme regulationi

    Inhibited by 5 mM of zinc, copper and iron. Unaffected by low concentrations of detergents and irreversibly inactivated by 2% ethanol, isopropanol or dimethyl sulfoxide.

    Kineticsi

    1. KM=25 µM for core histones1 Publication
    2. KM=9 µM for acetyl-CoA1 Publication

    pH dependencei

    Optimum pH is 8.2-8.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. H4 histone acetyltransferase activity Source: EnsemblPlants/Gramene

    GO - Biological processi

    1. chromatin silencing at telomere Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone acetyltransferase type B catalytic subunit (EC:2.3.1.48)
    Alternative name(s):
    Histone acetyltransferase HAT B
    Histone acetyltransferase HAT-B-p50
    Gene namesi
    Name:HAT1
    Synonyms:HAC106, HATB1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ8LPU4.
    MaizeGDBi273678.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: EnsemblPlants/Gramene
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Histone acetyltransferase type B catalytic subunitPRO_0000232126Add
    BLAST

    Proteomic databases

    PRIDEiQ8LPU4.

    Expressioni

    Developmental stagei

    Found in the dry embryo (at protein level). Maximum level of expression 22 hours after start of seed imbibition.1 Publication

    Interactioni

    Subunit structurei

    Heteromer of HAT1/p50 and p45 subunits.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LPU4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HAT1 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000084749.
    KOiK11303.

    Family and domain databases

    Gene3Di3.40.630.30. 1 hit.
    3.90.360.10. 1 hit.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR019467. Hat1_N.
    IPR017380. Hist_AcTrfase_B-typ_cat-su.
    [Graphical view]
    PANTHERiPTHR12046. PTHR12046. 1 hit.
    PfamiPF10394. Hat1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038084. HAT-B_cat. 1 hit.
    SUPFAMiSSF55729. SSF55729. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8LPU4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKQKDTDA AATATGTKKR RRVFFSDTDA GVEANECMKV FLVWNPGEVS    50
    SVDCTAIQPF DLNHFFGEDG KIYGYKNLKI NVWISAKSFH GYADVSFDET 100
    SDGGKGITDL KPVLQNIFGE NLVEKEEFLH TFSKECEYIR TAVTNGSAIK 150
    HDGSYESDPA VEIVRVELQG AAAFLYSRLV PLVLLLVEGS TPIDIGEHGW 200
    EMLLVVKKAT QEAGSKFELL GFAAVHNFYH YPESIRLRIS QILVLPPYQG 250
    EGHGLGLLEA INYIAQSENI YDVTIESPSD YLQYVRSSID CLRLLMFDPI 300
    KPALGAIVLS LKETNLSKRA QSLRMVPPAD LMETVRQKLK INKKQFLRCW 350
    EILVFLSLDS QDHKSMDNFR ACIYDRMKGE ILGSASGTNR KRLLQMPTSF 400
    NKEASFAVYW TQEIEDEDEQ TVEQQPEDLK TQEQQLNELV DIQIEEIAGV 450
    AKNVTSRCKD KMTELVVQ 468
    Length:468
    Mass (Da):52,721
    Last modified:April 4, 2006 - v2
    Checksum:i049F68157ED8443C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti354 – 3541V → I in AAM28228. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90274 mRNA. Translation: AAC03423.2.
    AF171927 Genomic DNA. Translation: AAF06742.1.
    AY093417 mRNA. Translation: AAM28228.1.
    PIRiT02064.
    RefSeqiNP_001105187.1. NM_001111717.1.
    UniGeneiZm.94647.

    Genome annotation databases

    GeneIDi542083.
    KEGGizma:542083.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90274 mRNA. Translation: AAC03423.2 .
    AF171927 Genomic DNA. Translation: AAF06742.1 .
    AY093417 mRNA. Translation: AAM28228.1 .
    PIRi T02064.
    RefSeqi NP_001105187.1. NM_001111717.1.
    UniGenei Zm.94647.

    3D structure databases

    ProteinModelPortali Q8LPU4.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q8LPU4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542083.
    KEGGi zma:542083.

    Organism-specific databases

    Gramenei Q8LPU4.
    MaizeGDBi 273678.

    Phylogenomic databases

    HOGENOMi HOG000084749.
    KOi K11303.

    Family and domain databases

    Gene3Di 3.40.630.30. 1 hit.
    3.90.360.10. 1 hit.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR019467. Hat1_N.
    IPR017380. Hist_AcTrfase_B-typ_cat-su.
    [Graphical view ]
    PANTHERi PTHR12046. PTHR12046. 1 hit.
    Pfami PF10394. Hat1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038084. HAT-B_cat. 1 hit.
    SUPFAMi SSF55729. SSF55729. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the histone acetyltransferase B complex of maize embryos."
      Lusser A., Eberharter A., Loidl A., Goralik-Schramel M., Horngacher M., Hass H., Loidl P.
      Nucleic Acids Res. 27:4427-4435(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH P45, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
      Strain: cv. Cuzco 251.
    2. "Sequences from the plant chromatin consortium."
      Chandler V.L., Kaeppler S.M., Kaeppler H.F., Cone K.C.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. B73.
    3. "Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos."
      Eberharter A., Lechner T., Goralik-Schramel M., Loidl P.
      FEBS Lett. 386:75-81(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Substrate and sequential site specificity of cytoplasmic histone acetyltransferases of maize and rat liver."
      Koelle D., Sarg B., Lindner H., Loidl P.
      FEBS Lett. 421:109-114(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY.

    Entry informationi

    Entry nameiHAT1_MAIZE
    AccessioniPrimary (citable) accession number: Q8LPU4
    Secondary accession number(s): O49994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3