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Q8LPU4 (HAT1_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone acetyltransferase type B catalytic subunit

EC=2.3.1.48
Alternative name(s):
Histone acetyltransferase HAT B
Histone acetyltransferase HAT-B-p50
Gene names
Name:HAT1
Synonyms:HAC106, HATB1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acetylates newly synthesized histones during DNA replication. Highly specific in vitro for the non-acetylated H4 which is acetylated sequentially at 'Lys-12' and 'Lys-5' into a di-acetylated form.

Catalytic activity

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Enzyme regulation

Inhibited by 5 mM of zinc, copper and iron. Unaffected by low concentrations of detergents and irreversibly inactivated by 2% ethanol, isopropanol or dimethyl sulfoxide.

Subunit structure

Heteromer of HAT1/p50 and p45 subunits.

Subcellular location

Nucleus. Cytoplasm Ref.1.

Developmental stage

Found in the dry embryo (at protein level). Maximum level of expression 22 hours after start of seed imbibition. Ref.1

Sequence similarities

Belongs to the HAT1 family.

Biophysicochemical properties

Kinetic parameters:

KM=25 µM for core histones Ref.3

KM=9 µM for acetyl-CoA

pH dependence:

Optimum pH is 8.2-8.5.

Temperature dependence:

Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processchromatin silencing at telomere

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionH4 histone acetyltransferase activity

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Histone acetyltransferase type B catalytic subunit
PRO_0000232126

Experimental info

Sequence conflict3541V → I in AAM28228. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8LPU4 [UniParc].

Last modified April 4, 2006. Version 2.
Checksum: 049F68157ED8443C

FASTA46852,721
        10         20         30         40         50         60 
MALKQKDTDA AATATGTKKR RRVFFSDTDA GVEANECMKV FLVWNPGEVS SVDCTAIQPF 

        70         80         90        100        110        120 
DLNHFFGEDG KIYGYKNLKI NVWISAKSFH GYADVSFDET SDGGKGITDL KPVLQNIFGE 

       130        140        150        160        170        180 
NLVEKEEFLH TFSKECEYIR TAVTNGSAIK HDGSYESDPA VEIVRVELQG AAAFLYSRLV 

       190        200        210        220        230        240 
PLVLLLVEGS TPIDIGEHGW EMLLVVKKAT QEAGSKFELL GFAAVHNFYH YPESIRLRIS 

       250        260        270        280        290        300 
QILVLPPYQG EGHGLGLLEA INYIAQSENI YDVTIESPSD YLQYVRSSID CLRLLMFDPI 

       310        320        330        340        350        360 
KPALGAIVLS LKETNLSKRA QSLRMVPPAD LMETVRQKLK INKKQFLRCW EILVFLSLDS 

       370        380        390        400        410        420 
QDHKSMDNFR ACIYDRMKGE ILGSASGTNR KRLLQMPTSF NKEASFAVYW TQEIEDEDEQ 

       430        440        450        460 
TVEQQPEDLK TQEQQLNELV DIQIEEIAGV AKNVTSRCKD KMTELVVQ 

« Hide

References

[1]"Analysis of the histone acetyltransferase B complex of maize embryos."
Lusser A., Eberharter A., Loidl A., Goralik-Schramel M., Horngacher M., Hass H., Loidl P.
Nucleic Acids Res. 27:4427-4435(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH P45, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
Strain: cv. Cuzco 251.
[2]"Sequences from the plant chromatin consortium."
Chandler V.L., Kaeppler S.M., Kaeppler H.F., Cone K.C.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. B73.
[3]"Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos."
Eberharter A., Lechner T., Goralik-Schramel M., Loidl P.
FEBS Lett. 386:75-81(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.
[4]"Substrate and sequential site specificity of cytoplasmic histone acetyltransferases of maize and rat liver."
Koelle D., Sarg B., Lindner H., Loidl P.
FEBS Lett. 421:109-114(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U90274 mRNA. Translation: AAC03423.2.
AF171927 Genomic DNA. Translation: AAF06742.1.
AY093417 mRNA. Translation: AAM28228.1.
PIRT02064.
RefSeqNP_001105187.1. NM_001111717.1.
UniGeneZm.94647.

3D structure databases

ProteinModelPortalQ8LPU4.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8LPU4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542083.
KEGGzma:542083.

Organism-specific databases

GrameneQ8LPU4.
MaizeGDB273678.

Phylogenomic databases

HOGENOMHOG000084749.
KOK11303.

Family and domain databases

Gene3D3.40.630.30. 1 hit.
3.90.360.10. 1 hit.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR019467. Hat1_N.
IPR017380. Hist_AcTrfase_B-typ_cat-su.
[Graphical view]
PANTHERPTHR12046. PTHR12046. 1 hit.
PfamPF10394. Hat1_N. 1 hit.
[Graphical view]
PIRSFPIRSF038084. HAT-B_cat. 1 hit.
SUPFAMSSF55729. SSF55729. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHAT1_MAIZE
AccessionPrimary (citable) accession number: Q8LPU4
Secondary accession number(s): O49994
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: July 9, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families