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Q8LPU4

- HAT1_MAIZE

UniProt

Q8LPU4 - HAT1_MAIZE

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Protein

Histone acetyltransferase type B catalytic subunit

Gene

HAT1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acetylates newly synthesized histones during DNA replication. Highly specific in vitro for the non-acetylated H4 which is acetylated sequentially at 'Lys-12' and 'Lys-5' into a di-acetylated form.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].By similarity

Enzyme regulationi

Inhibited by 5 mM of zinc, copper and iron. Unaffected by low concentrations of detergents and irreversibly inactivated by 2% ethanol, isopropanol or dimethyl sulfoxide.

Kineticsi

  1. KM=25 µM for core histones1 Publication
  2. KM=9 µM for acetyl-CoA1 Publication

pH dependencei

Optimum pH is 8.2-8.5.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei200 – 2001Interaction with histone H4 N-terminusBy similarity
Active sitei276 – 2761Proton donor/acceptorBy similarity

GO - Molecular functioni

  1. histone acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. chromatin silencing at telomere Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase type B catalytic subunit (EC:2.3.1.48By similarity)
Alternative name(s):
Histone acetyltransferase HAT B
Histone acetyltransferase HAT-B-p50
Gene namesi
Name:HAT1
Synonyms:HAC106, HATB1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ8LPU4.
MaizeGDBi273678.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Histone acetyltransferase type B catalytic subunitPRO_0000232126Add
BLAST

Proteomic databases

PRIDEiQ8LPU4.

Expressioni

Developmental stagei

Found in the dry embryo (at protein level). Maximum level of expression 22 hours after start of seed imbibition.1 Publication

Interactioni

Subunit structurei

Heteromer of HAT1/p50 and p45 subunits.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8LPU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 2443Acetyl-CoA bindingBy similarity
Regioni249 – 2557Acetyl-CoA bindingBy similarity

Sequence similaritiesi

Belongs to the HAT1 family.Curated

Phylogenomic databases

HOGENOMiHOG000084749.
KOiK11303.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
3.90.360.10. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR019467. Hat1_N.
IPR017380. Hist_AcTrfase_B-typ_cat-su.
[Graphical view]
PANTHERiPTHR12046. PTHR12046. 1 hit.
PfamiPF10394. Hat1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF038084. HAT-B_cat. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8LPU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKQKDTDA AATATGTKKR RRVFFSDTDA GVEANECMKV FLVWNPGEVS
60 70 80 90 100
SVDCTAIQPF DLNHFFGEDG KIYGYKNLKI NVWISAKSFH GYADVSFDET
110 120 130 140 150
SDGGKGITDL KPVLQNIFGE NLVEKEEFLH TFSKECEYIR TAVTNGSAIK
160 170 180 190 200
HDGSYESDPA VEIVRVELQG AAAFLYSRLV PLVLLLVEGS TPIDIGEHGW
210 220 230 240 250
EMLLVVKKAT QEAGSKFELL GFAAVHNFYH YPESIRLRIS QILVLPPYQG
260 270 280 290 300
EGHGLGLLEA INYIAQSENI YDVTIESPSD YLQYVRSSID CLRLLMFDPI
310 320 330 340 350
KPALGAIVLS LKETNLSKRA QSLRMVPPAD LMETVRQKLK INKKQFLRCW
360 370 380 390 400
EILVFLSLDS QDHKSMDNFR ACIYDRMKGE ILGSASGTNR KRLLQMPTSF
410 420 430 440 450
NKEASFAVYW TQEIEDEDEQ TVEQQPEDLK TQEQQLNELV DIQIEEIAGV
460
AKNVTSRCKD KMTELVVQ
Length:468
Mass (Da):52,721
Last modified:April 4, 2006 - v2
Checksum:i049F68157ED8443C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti354 – 3541V → I in AAM28228. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U90274 mRNA. Translation: AAC03423.2.
AF171927 Genomic DNA. Translation: AAF06742.1.
AY093417 mRNA. Translation: AAM28228.1.
PIRiT02064.
RefSeqiNP_001105187.1. NM_001111717.1.
UniGeneiZm.94647.

Genome annotation databases

GeneIDi542083.
KEGGizma:542083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U90274 mRNA. Translation: AAC03423.2 .
AF171927 Genomic DNA. Translation: AAF06742.1 .
AY093417 mRNA. Translation: AAM28228.1 .
PIRi T02064.
RefSeqi NP_001105187.1. NM_001111717.1.
UniGenei Zm.94647.

3D structure databases

ProteinModelPortali Q8LPU4.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q8LPU4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542083.
KEGGi zma:542083.

Organism-specific databases

Gramenei Q8LPU4.
MaizeGDBi 273678.

Phylogenomic databases

HOGENOMi HOG000084749.
KOi K11303.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
3.90.360.10. 1 hit.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR019467. Hat1_N.
IPR017380. Hist_AcTrfase_B-typ_cat-su.
[Graphical view ]
PANTHERi PTHR12046. PTHR12046. 1 hit.
Pfami PF10394. Hat1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF038084. HAT-B_cat. 1 hit.
SUPFAMi SSF55729. SSF55729. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Analysis of the histone acetyltransferase B complex of maize embryos."
    Lusser A., Eberharter A., Loidl A., Goralik-Schramel M., Horngacher M., Hass H., Loidl P.
    Nucleic Acids Res. 27:4427-4435(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH P45, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
    Strain: cv. Cuzco 251.
  2. "Sequences from the plant chromatin consortium."
    Chandler V.L., Kaeppler S.M., Kaeppler H.F., Cone K.C.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. B73.
  3. "Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos."
    Eberharter A., Lechner T., Goralik-Schramel M., Loidl P.
    FEBS Lett. 386:75-81(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Substrate and sequential site specificity of cytoplasmic histone acetyltransferases of maize and rat liver."
    Koelle D., Sarg B., Lindner H., Loidl P.
    FEBS Lett. 421:109-114(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiHAT1_MAIZE
AccessioniPrimary (citable) accession number: Q8LPU4
Secondary accession number(s): O49994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: October 29, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3