Reviewed,
UniProtKB/Swiss-Prot Q8LPU4 (HAT1_MAIZE)
Last modified
February 9, 2010.
Version 34.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Histone acetyltransferase type B catalytic subunit EC=2.3.1.48 Alternative name(s): Histone acetyltransferase HAT B Histone acetyltransferase HAT-B-p50 | ||||
| Gene names |
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| Organism | Zea mays (Maize) | ||||
| Taxonomic identifier | 4577 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea |
Protein attributes
| Sequence length | 468 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acetylates newly synthesized histones during DNA replication. Highly specific in vitro for the non-acetylated H4 which is acetylated sequentially at 'Lys-12' and 'Lys-5' into a di-acetylated form. |
| Catalytic activity | Acetyl-CoA + [histone] = CoA + acetyl-[histone]. |
| Enzyme regulation | Inhibited by 5 mM of zinc, copper and iron. Unaffected by low concentrations of detergents and irreversibly inactivated by 2% ethanol, isopropanol or dimethyl sulfoxide. |
| Subunit structure | Heteromer of HAT1/p50 and p45 subunits. |
| Subcellular location | |
| Developmental stage | Found in the dry embryo (at protein level). Maximum level of expression 22 hours after start of seed imbibition. Ref.1 |
| Sequence similarities | Belongs to the HAT1 family. |
| Biophysicochemical properties | Kinetic parameters: KM=25 µM for core histones KM=9 µM for acetyl-CoA pH dependence: Optimum pH is 8.2-8.5. Temperature dependence: Optimum temperature is 37 degrees Celsius. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| Molecular function | Acyltransferase Transferase |
| Gene Ontology (GO) | |
| Biological process | chromatin silencing at telomere Inferred from electronic annotation. Source: InterPro histone acetylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | histone acetyltransferase activity Inferred from electronic annotation. Source: EC protein bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Analysis of the histone acetyltransferase B complex of maize embryos." Lusser A., Eberharter A., Loidl A., Goralik-Schramel M., Horngacher M., Hass H., Loidl P. Nucleic Acids Res. 27:4427-4435(1999) [PubMed: 10536152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH P45, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION. Strain: cv. Cuzco 251. |
| [2] | "Sequences from the plant chromatin consortium." Chandler V.L., Kaeppler S.M., Kaeppler H.F., Cone K.C. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. B73. |
| [3] | "Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos." Eberharter A., Lechner T., Goralik-Schramel M., Loidl P. FEBS Lett. 386:75-81(1996) [PubMed: 8635608] [Abstract] Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES. |
| [4] | "Substrate and sequential site specificity of cytoplasmic histone acetyltransferases of maize and rat liver." Koelle D., Sarg B., Lindner H., Loidl P. FEBS Lett. 421:109-114(1998) [PubMed: 9468289] [Abstract] Cited for: SUBSTRATE SPECIFICITY. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U90274 mRNA. Translation: AAC03423.2. AF171927 Genomic DNA. Translation: AAF06742.1. AY093417 mRNA. Translation: AAM28228.1. |
| PIR | T02064. |
| RefSeq | NP_001105187.1. |
| UniGene | Zm.94647 |
3D structure databases | |
| SMR | Q8LPU4. Positions 29-359. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 542083. |
| KEGG | zma:542083. |
Organism-specific databases | |
| Gramene | Q8LPU4. |
| MaizeGDB | 273678. |
Enzyme and pathway databases | |
| BRENDA | 2.3.1.48. 289. |
Family and domain databases | |
| InterPro | IPR016181. Acyl_CoA_acyltransferase. IPR019467. Hat1_N. IPR017380. Hist_AcTrfase_B-typ_cat-su. [Graphical view] |
| Gene3D | G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit. |
| Pfam | PF10394. Hat1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF038084. HAT-B_cat. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | HAT1_MAIZE | ||||||||
| Accession | Primary (citable) accession number: Q8LPU4 Secondary accession number(s): O49994 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


