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Q8LPS1

- LACS6_ARATH

UniProt

Q8LPS1 - LACS6_ARATH

Protein

Long chain acyl-CoA synthetase 6, peroxisomal

Gene

LACS6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate. Might play a regulatory role both in fatty acid import into glyoxysomes and in fatty acid beta-oxidation. Displays redundant function with LACS7 into the seed development process.2 Publications

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi266 – 27712ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB
    2. multicellular organismal development Source: UniProtKB-KW
    3. response to ozone Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G05970-MONOMER.
    MetaCyc:AT3G05970-MONOMER.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 6, peroxisomal (EC:6.2.1.3)
    Gene namesi
    Name:LACS6
    Ordered Locus Names:At3g05970
    ORF Names:F2O10.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G05970.

    Subcellular locationi

    Peroxisome 2 Publications. Glyoxysome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. glyoxysomal membrane Source: UniProtKB-SubCell
    2. glyoxysome Source: UniProtKB-KW
    3. membrane Source: TAIR
    4. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Glyoxysome, Membrane, Peroxisome

    Pathology & Biotechi

    Disruption phenotypei

    Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids into beta-oxidation, a necessary process to pursue the development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 3838Removed in mature form1 PublicationPRO_0000401414Add
    BLAST
    Chaini39 – 701663Long chain acyl-CoA synthetase 6, peroxisomalPRO_0000401415Add
    BLAST

    Proteomic databases

    PaxDbiQ8LPS1.
    PRIDEiQ8LPS1.

    Expressioni

    Tissue specificityi

    Preferentially expressed in seeds and senescent leaves.1 Publication

    Developmental stagei

    Induced during seed germination.1 Publication

    Inductioni

    Up-regulated by ozone.1 Publication

    Gene expression databases

    GenevestigatoriQ8LPS1.

    Interactioni

    Protein-protein interaction databases

    BioGridi5102. 1 interaction.
    STRINGi3702.AT3G05970.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LPS1.
    SMRiQ8LPS1. Positions 119-569.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni526 – 55025Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 239Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ8LPS1.
    KOiK01897.
    OMAiMDAVGRE.
    PhylomeDBiQ8LPS1.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LPS1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSSSSSSSA AARRRINAIH SHLVTSSRSS PLLRSNPTAG EFCLDNGYSV    50
    VLPEKLNTGS WNVYRSAKSP FKLVSRFPDH PDIATLHDNF EHAVHDFRDY 100
    KYLGTRVRVD GTVGDYKWMT YGEAGTARTA LGSGLVHHGI PMGSSVGIYF 150
    INRPEWLIVD HACSSYSYVS VPLYDTLGPD AVKFIVNHAT VQAIFCVAET 200
    LNSLLSCLSE MPSVRLVVVV GGLIESLPSL PSSSGVKVVS YSVLLNQGRS 250
    NPQRFFPPKP DDVATICYTS GTTGTPKGVV LTHANLIANV AGSSFSVKFF 300
    SSDVYISYLP LAHIYERANQ ILTVYFGVAV GFYQGDNMKL LDDLAALRPT 350
    VFSSVPRLYN RIYAGIINAV KTSGGLKERL FNAAYNAKKQ ALLNGKSASP 400
    IWDRLVFNKI KDRLGGRVRF MTSGASPLSP EVMEFLKVCF GGRVTEGYGM 450
    TETSCVISGM DEGDNLTGHV GSPNPACEVK LVDVPEMNYT SADQPHPRGE 500
    ICVRGPIIFT GYYKDEIQTK EVIDEDGWLH TGDIGLWLPG GRLKIIDRKK 550
    NIFKLAQGEY IAPEKIENVY AKCKFVGQCF IYGDSFNSSL VAVVSVDPDV 600
    LKSWAASEGI KGGDLRELCN NPRVKAAVLS DMDTVGREAQ LRGFEFAKAV 650
    TLVLEPFTLE NGLLTPTFKI KRPQAKEYFA EAITNMYKEL GASDPSANRG 700
    L 701
    Length:701
    Mass (Da):76,603
    Last modified:October 1, 2002 - v1
    Checksum:i4A0F06A05FA13022
    GO

    Sequence cautioni

    The sequence AAF23219.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601S → N in BAB40450. (PubMed:12481085)Curated
    Sequence conflicti139 – 1391G → V in AAM28873. (PubMed:12177484)Curated
    Sequence conflicti224 – 2241I → N in BAB40450. (PubMed:12481085)Curated
    Sequence conflicti479 – 4791V → I in AAM28873. (PubMed:12177484)Curated
    Sequence conflicti517 – 5171I → V in BAB40450. (PubMed:12481085)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503756 mRNA. Translation: AAM28873.1.
    AB030317 mRNA. Translation: BAB40450.1.
    AC013454 Genomic DNA. Translation: AAF23219.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74324.1.
    AY094418 mRNA. Translation: AAM19792.1.
    BT001117 mRNA. Translation: AAN64508.1.
    RefSeqiNP_566265.1. NM_111471.2.
    UniGeneiAt.25229.

    Genome annotation databases

    EnsemblPlantsiAT3G05970.1; AT3G05970.1; AT3G05970.
    GeneIDi819767.
    KEGGiath:AT3G05970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503756 mRNA. Translation: AAM28873.1 .
    AB030317 mRNA. Translation: BAB40450.1 .
    AC013454 Genomic DNA. Translation: AAF23219.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74324.1 .
    AY094418 mRNA. Translation: AAM19792.1 .
    BT001117 mRNA. Translation: AAN64508.1 .
    RefSeqi NP_566265.1. NM_111471.2.
    UniGenei At.25229.

    3D structure databases

    ProteinModelPortali Q8LPS1.
    SMRi Q8LPS1. Positions 119-569.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5102. 1 interaction.
    STRINGi 3702.AT3G05970.1-P.

    Proteomic databases

    PaxDbi Q8LPS1.
    PRIDEi Q8LPS1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G05970.1 ; AT3G05970.1 ; AT3G05970 .
    GeneIDi 819767.
    KEGGi ath:AT3G05970.

    Organism-specific databases

    GeneFarmi 2576. 207.
    TAIRi AT3G05970.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q8LPS1.
    KOi K01897.
    OMAi MDAVGRE.
    PhylomeDBi Q8LPS1.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT3G05970-MONOMER.
    MetaCyc:AT3G05970-MONOMER.

    Gene expression databases

    Genevestigatori Q8LPS1.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. "Molecular characterization of an Arabidopsis acyl-coenzyme A synthetase localized on glyoxysomal membranes."
      Hayashi H., De Bellis L., Hayashi Y., Nito K., Kato A., Hayashi M., Hara-Nishimura I., Nishimura M.
      Plant Physiol. 130:2019-2026(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-60, FUNCTION, SUBCELLULAR LOCATION.
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Two long-chain acyl-CoA synthetases from Arabidopsis thaliana involved in peroxisomal fatty acid beta-oxidation."
      Fulda M., Shockey J., Werber M., Wolter F.P., Heinz E.
      Plant J. 32:93-103(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, ENZYME ACTIVITY.
    7. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.
    8. "Peroxisomal Acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana."
      Fulda M., Schnurr J., Abbadi A., Heinz E., Browse J.
      Plant Cell 16:394-405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    9. "AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5."
      Bonsegna S., Slocombe S.P., De Bellis L., Baker A.
      Arch. Biochem. Biophys. 443:74-81(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiLACS6_ARATH
    AccessioniPrimary (citable) accession number: Q8LPS1
    Secondary accession number(s): Q8LKS6, Q9C5U7, Q9SFG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3