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Q8LPR9

- TI110_ARATH

UniProt

Q8LPR9 - TI110_ARATH

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Protein

Protein TIC110, chloroplastic

Gene
TIC110, At1g06950, F10K1.33, F4H5.1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in protein precursor import into chloroplasts. Forms a voltage-dependent cation-selective channel at the inner envelope of chloroplasts, which specifically responds to a transit peptide. Associates with both the precursor and mature forms of the preprotein.4 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. chloroplast organization Source: TAIR
  2. protein import into chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Protein family/group databases

TCDBi3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIC110, chloroplastic
Alternative name(s):
Translocon at the inner envelope membrane of chloroplasts 110
Short name:
AtTIC110
Gene namesi
Name:TIC110
Ordered Locus Names:At1g06950
ORF Names:F10K1.33, F4H5.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G06950.

Subcellular locationi

Plastidchloroplast inner membrane; Multi-pass membrane protein Reviewed prediction 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini51 – 9545Stromal By similarityAdd
BLAST
Transmembranei96 – 11621Helical; Reviewed predictionAdd
BLAST
Topological domaini117 – 1182Chloroplast intermembrane By similarity
Transmembranei119 – 13921Helical; Reviewed predictionAdd
BLAST
Topological domaini140 – 259120Stromal By similarityAdd
BLAST
Transmembranei260 – 28021Helical; Reviewed predictionAdd
BLAST
Topological domaini281 – 36888Chloroplast intermembrane By similarityAdd
BLAST
Transmembranei369 – 38618Helical; By similarityAdd
BLAST
Topological domaini387 – 632246Stromal By similarityAdd
BLAST
Transmembranei633 – 65018Helical; By similarityAdd
BLAST
Topological domaini651 – 71969Chloroplast intermembrane By similarityAdd
BLAST
Transmembranei720 – 73617Helical; By similarityAdd
BLAST
Topological domaini737 – 1016280Stromal By similarityAdd
BLAST

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: TAIR
  5. plastid Source: TAIR
  6. Tic complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid inner membrane

Pathology & Biotechi

Disruption phenotypei

Embryo lethal.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Chloroplast Reviewed predictionAdd
BLAST
Chaini51 – 1016966Protein TIC110, chloroplasticPRO_0000413668Add
BLAST

Proteomic databases

PRIDEiQ8LPR9.

Expressioni

Tissue specificityi

Expressed in seedlings, flowers, leaves, stems and roots.3 Publications

Developmental stagei

Expressed throughout development.1 Publication

Gene expression databases

GenevestigatoriQ8LPR9.

Interactioni

Subunit structurei

Part of the Tic complex. Interacts with HSP70, HSP93 and TIC40. Interacts with the Toc complex components TOC33, TOC75 and TOC159. Interacts with LTD.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIC40Q9FMD53EBI-639092,EBI-639157

Protein-protein interaction databases

BioGridi22446. 9 interactions.
IntActiQ8LPR9. 7 interactions.
STRINGi3702.AT1G06950.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LPR9.

Family & Domainsi

Domaini

An internal domain (143-652) is sufficient for assembly into supercomplex with Toc complex.
Residues 235-420 contain a binding site for preprotein transit peptides.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259340.
HOGENOMiHOG000243512.
InParanoidiQ8LPR9.
OMAiHMEIGRR.
PhylomeDBiQ8LPR9.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LPR9-1 [UniParc]FASTAAdd to Basket

« Hide

MNPSLVTAIN APISPSPRSP LLSHFLPTLP HRFSKSECLS RRRYRVSFPR     50
SSAASSDQLS VSTQAKNPGI HGNKKELTGL QPIVEKMTPP VRLATSAVVL 100
AASLATGYGL GLRLAGSRNI AFGGAAVAGA AGGAVVYALN SAVPEVAAIS 150
LHNYVAEFED PASVTKDDVE KIADRYGVNK GDEAFQAEIC DIYCRYVTSV 200
LPTEGQSLKG DEVAKIVKFK NALGIDEPDA AAMHMEIGRR IFRQRLETGE 250
REGDAEQRRA FMRLVYVSAL VFGDASSFLL PWKRVLKVTD AQVEIAIREN 300
AKQLYAERLK LVGRDINVEN LVDLRKSQLS FKLSDELAED LFREHTRKVV 350
VENISSALSI LKSRTRAAKS LASVVEELEK VLEFNNLLVS LKSHSEADQF 400
ARGVGPISLI GDESDFERRM DDLKLLYRAY VTDALSGGRL EENKLVAMSQ 450
LRNILGLGKR EAEAISVDVT SKSYRKRLAN AVSSGDLEAQ DSKAKYLQKL 500
CEELHFDAQK AGAIHEEIYR QKLQQCVTDG ELSDDNVAAL LRLRVMLCIP 550
QQTVDTAHAE ICGTIFEKVV RDAISSGVDG YDAETRKSVR KAAHGLRLSR 600
ETAMSIASKA VRRVFTNYIR RARAAENRTD SAKELKKMIA FNTLVVTEMV 650
ADIKGESSDK APEEDPVQEK EEDDEDEEWG SLESLRKTRP DKELAEKMGK 700
PGQTEITLKD DLPDRDRIDL YKTYLLYCVT GEVTRIPFGA QITTKRDDSE 750
YLLLNQLGGI LGLSSKEIVN IHVGLAEQAF RQQAEVILAD GQLTKARVEQ 800
LDELQKQVGL PQPQAEKVIK NITTTKMANA IETAVNQGRL NIKQIRELKE 850
ANVSLDSMIA VSLREKLFKK TVSDIFSSGT GEFDETEVYQ TIPSDLSIDV 900
EKAKRVVHDL AQSRLSNSLV QAVALLRQRN SKGVVLSLND LLACDKAVPA 950
EPMSWEVSEE LSDLYAIYSK SDPKPAPEKV LRLQYLLGID DSTATALREM 1000
EDGALSSAAE EGNFVF 1016
Length:1,016
Mass (Da):112,121
Last modified:October 1, 2002 - v1
Checksum:i814CCDE07EB5D0EA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201K → N in AAM20701. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011001 Genomic DNA. Translation: AAF63131.1.
AC067971 Genomic DNA. Translation: AAF82224.1.
CP002684 Genomic DNA. Translation: AEE28057.1.
AY094426 mRNA. Translation: AAM19799.1.
AY099850 mRNA. Translation: AAM20701.1.
AK229869 mRNA. Translation: BAF01698.1.
PIRiB86204.
RefSeqiNP_172176.1. NM_100568.3.
UniGeneiAt.12081.

Genome annotation databases

EnsemblPlantsiAT1G06950.1; AT1G06950.1; AT1G06950.
GeneIDi837205.
KEGGiath:AT1G06950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011001 Genomic DNA. Translation: AAF63131.1 .
AC067971 Genomic DNA. Translation: AAF82224.1 .
CP002684 Genomic DNA. Translation: AEE28057.1 .
AY094426 mRNA. Translation: AAM19799.1 .
AY099850 mRNA. Translation: AAM20701.1 .
AK229869 mRNA. Translation: BAF01698.1 .
PIRi B86204.
RefSeqi NP_172176.1. NM_100568.3.
UniGenei At.12081.

3D structure databases

ProteinModelPortali Q8LPR9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 22446. 9 interactions.
IntActi Q8LPR9. 7 interactions.
STRINGi 3702.AT1G06950.1-P.

Protein family/group databases

TCDBi 3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

Proteomic databases

PRIDEi Q8LPR9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G06950.1 ; AT1G06950.1 ; AT1G06950 .
GeneIDi 837205.
KEGGi ath:AT1G06950.

Organism-specific databases

TAIRi AT1G06950.

Phylogenomic databases

eggNOGi NOG259340.
HOGENOMi HOG000243512.
InParanoidi Q8LPR9.
OMAi HMEIGRR.
PhylomeDBi Q8LPR9.

Gene expression databases

Genevestigatori Q8LPR9.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 823-1016.
    Strain: cv. Columbia.
  5. "The hydrophilic domain of Tic110, an inner envelope membrane component of the chloroplastic protein translocation apparatus, faces the stromal compartment."
    Jackson D.T., Froehlich J.E., Keegstra K.
    J. Biol. Chem. 273:16583-16588(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  6. "The preprotein conducting channel at the inner envelope membrane of plastids."
    Heins L., Mehrle A., Hemmler R., Wagner R., Kuechler M., Hoermann F., Sveshnikov D., Soll J.
    EMBO J. 21:2616-2625(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "atTic110 functions as a scaffold for coordinating the stromal events of protein import into chloroplasts."
    Inaba T., Li M., Alvarez-Huerta M., Kessler F., Schnell D.J.
    J. Biol. Chem. 278:38617-38627(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOPOLOGY.
  8. Cited for: TISSUE SPECIFICITY.
  9. "Arabidopsis tic110 is essential for the assembly and function of the protein import machinery of plastids."
    Inaba T., Alvarez-Huerta M., Li M., Bauer J., Ewers C., Kessler F., Schnell D.J.
    Plant Cell 17:1482-1496(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH HSP93; TIC40 AND TOC COMPLEX.
  10. "In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import."
    Kovacheva S., Bedard J., Patel R., Dudley P., Twell D., Rios G., Koncz C., Jarvis P.
    Plant J. 41:412-428(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  11. "Reconstitution of protein targeting to the inner envelope membrane of chloroplasts."
    Li M., Schnell D.J.
    J. Cell Biol. 175:249-259(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "Precursor binding to an 880-kDa Toc complex as an early step during active import of protein into chloroplasts."
    Chen K.Y., Li H.M.
    Plant J. 49:149-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN TIC/TOC SUPERCOMPLEX.
  13. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts."
    Su P.H., Li H.M.
    Plant Cell 22:1516-1531(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSP70.
  15. "LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway."
    Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.
    Nat. Commun. 2:277-277(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LTD.
  16. "Protein import into chloroplasts: the Tic complex and its regulation."
    Kovacs-Bogdan E., Soll J., Bolter B.
    Biochim. Biophys. Acta 1803:740-747(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiTI110_ARATH
AccessioniPrimary (citable) accession number: Q8LPR9
Secondary accession number(s): Q0WMF8
, Q8LPG5, Q9LMI9, Q9M9Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The region 143-1016 is indicated to be located in the stroma (1 Publication, 1 Publication) while the homologous region in pea TIC110 contains probably 4 trans-membrane domains resulting in 2 regions in the intermembrane space localized to form supercomplexes with the TOC machinery and to receive the transit peptide of preproteins.
Inserts into the inner envelope membrane from the stroma after import from the cytoplasm.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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