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Q8LPR9 (TI110_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein TIC110, chloroplastic
Alternative name(s):
Translocon at the inner envelope membrane of chloroplasts 110
Short name=AtTIC110
Gene names
Name:TIC110
Ordered Locus Names:At1g06950
ORF Names:F10K1.33, F4H5.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1016 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein precursor import into chloroplasts. Forms a voltage-dependent cation-selective channel at the inner envelope of chloroplasts, which specifically responds to a transit peptide. Associates with both the precursor and mature forms of the preprotein. Ref.6 Ref.7 Ref.9 Ref.10

Subunit structure

Part of the Tic complex. Interacts with HSP70, HSP93 and TIC40. Interacts with the Toc complex components TOC33, TOC75 and TOC159. Interacts with LTD. Ref.9 Ref.12 Ref.14 Ref.15

Subcellular location

Plastidchloroplast inner membrane; Multi-pass membrane protein Potential Ref.11.

Tissue specificity

Expressed in seedlings, flowers, leaves, stems and roots. Ref.8 Ref.9 Ref.10

Developmental stage

Expressed throughout development. Ref.10

Domain

An internal domain (143-652) is sufficient for assembly into supercomplex with Toc complex.

Residues 235-420 contain a binding site for preprotein transit peptides.

Disruption phenotype

Embryo lethal. Ref.9 Ref.10

Miscellaneous

The region 143-1016 is indicated to be located in the stroma (Ref.7, Ref.5) while the homologous region in pea TIC110 contains probably 4 trans-membrane domains resulting in 2 regions in the intermembrane space localized to form supercomplexes with the TOC machinery and to receive the transit peptide of preproteins.

Inserts into the inner envelope membrane from the stroma after import from the cytoplasm.

Sequence similarities

Belongs to the chloroplast envelope anion channel-forming Tic110 (TC 1.A.18) family. [View classification]

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIC40Q9FMD53EBI-639092,EBI-639157

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Potential
Chain51 – 1016966Protein TIC110, chloroplastic
PRO_0000413668

Regions

Topological domain51 – 9545Stromal By similarity
Transmembrane96 – 11621Helical; Potential
Topological domain117 – 1182Chloroplast intermembrane By similarity
Transmembrane119 – 13921Helical; Potential
Topological domain140 – 259120Stromal By similarity
Transmembrane260 – 28021Helical; Potential
Topological domain281 – 36888Chloroplast intermembrane By similarity
Transmembrane369 – 38618Helical; By similarity
Topological domain387 – 632246Stromal By similarity
Transmembrane633 – 65018Helical; By similarity
Topological domain651 – 71969Chloroplast intermembrane By similarity
Transmembrane720 – 73617Helical; By similarity
Topological domain737 – 1016280Stromal By similarity

Experimental info

Sequence conflict2201K → N in AAM20701. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8LPR9 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 814CCDE07EB5D0EA

FASTA1,016112,121
        10         20         30         40         50         60 
MNPSLVTAIN APISPSPRSP LLSHFLPTLP HRFSKSECLS RRRYRVSFPR SSAASSDQLS 

        70         80         90        100        110        120 
VSTQAKNPGI HGNKKELTGL QPIVEKMTPP VRLATSAVVL AASLATGYGL GLRLAGSRNI 

       130        140        150        160        170        180 
AFGGAAVAGA AGGAVVYALN SAVPEVAAIS LHNYVAEFED PASVTKDDVE KIADRYGVNK 

       190        200        210        220        230        240 
GDEAFQAEIC DIYCRYVTSV LPTEGQSLKG DEVAKIVKFK NALGIDEPDA AAMHMEIGRR 

       250        260        270        280        290        300 
IFRQRLETGE REGDAEQRRA FMRLVYVSAL VFGDASSFLL PWKRVLKVTD AQVEIAIREN 

       310        320        330        340        350        360 
AKQLYAERLK LVGRDINVEN LVDLRKSQLS FKLSDELAED LFREHTRKVV VENISSALSI 

       370        380        390        400        410        420 
LKSRTRAAKS LASVVEELEK VLEFNNLLVS LKSHSEADQF ARGVGPISLI GDESDFERRM 

       430        440        450        460        470        480 
DDLKLLYRAY VTDALSGGRL EENKLVAMSQ LRNILGLGKR EAEAISVDVT SKSYRKRLAN 

       490        500        510        520        530        540 
AVSSGDLEAQ DSKAKYLQKL CEELHFDAQK AGAIHEEIYR QKLQQCVTDG ELSDDNVAAL 

       550        560        570        580        590        600 
LRLRVMLCIP QQTVDTAHAE ICGTIFEKVV RDAISSGVDG YDAETRKSVR KAAHGLRLSR 

       610        620        630        640        650        660 
ETAMSIASKA VRRVFTNYIR RARAAENRTD SAKELKKMIA FNTLVVTEMV ADIKGESSDK 

       670        680        690        700        710        720 
APEEDPVQEK EEDDEDEEWG SLESLRKTRP DKELAEKMGK PGQTEITLKD DLPDRDRIDL 

       730        740        750        760        770        780 
YKTYLLYCVT GEVTRIPFGA QITTKRDDSE YLLLNQLGGI LGLSSKEIVN IHVGLAEQAF 

       790        800        810        820        830        840 
RQQAEVILAD GQLTKARVEQ LDELQKQVGL PQPQAEKVIK NITTTKMANA IETAVNQGRL 

       850        860        870        880        890        900 
NIKQIRELKE ANVSLDSMIA VSLREKLFKK TVSDIFSSGT GEFDETEVYQ TIPSDLSIDV 

       910        920        930        940        950        960 
EKAKRVVHDL AQSRLSNSLV QAVALLRQRN SKGVVLSLND LLACDKAVPA EPMSWEVSEE 

       970        980        990       1000       1010 
LSDLYAIYSK SDPKPAPEKV LRLQYLLGID DSTATALREM EDGALSSAAE EGNFVF 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 823-1016.
Strain: cv. Columbia.
[5]"The hydrophilic domain of Tic110, an inner envelope membrane component of the chloroplastic protein translocation apparatus, faces the stromal compartment."
Jackson D.T., Froehlich J.E., Keegstra K.
J. Biol. Chem. 273:16583-16588(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[6]"The preprotein conducting channel at the inner envelope membrane of plastids."
Heins L., Mehrle A., Hemmler R., Wagner R., Kuechler M., Hoermann F., Sveshnikov D., Soll J.
EMBO J. 21:2616-2625(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"atTic110 functions as a scaffold for coordinating the stromal events of protein import into chloroplasts."
Inaba T., Li M., Alvarez-Huerta M., Kessler F., Schnell D.J.
J. Biol. Chem. 278:38617-38627(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TOPOLOGY.
[8]"The protein translocon of the plastid envelopes."
Vojta A., Alavi M., Becker T., Hoermann F., Kuechler M., Soll J., Thomson R., Schleiff E.
J. Biol. Chem. 279:21401-21405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Arabidopsis tic110 is essential for the assembly and function of the protein import machinery of plastids."
Inaba T., Alvarez-Huerta M., Li M., Bauer J., Ewers C., Kessler F., Schnell D.J.
Plant Cell 17:1482-1496(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH HSP93; TIC40 AND TOC COMPLEX.
[10]"In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import."
Kovacheva S., Bedard J., Patel R., Dudley P., Twell D., Rios G., Koncz C., Jarvis P.
Plant J. 41:412-428(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[11]"Reconstitution of protein targeting to the inner envelope membrane of chloroplasts."
Li M., Schnell D.J.
J. Cell Biol. 175:249-259(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[12]"Precursor binding to an 880-kDa Toc complex as an early step during active import of protein into chloroplasts."
Chen K.Y., Li H.M.
Plant J. 49:149-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN TIC/TOC SUPERCOMPLEX.
[13]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts."
Su P.H., Li H.M.
Plant Cell 22:1516-1531(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HSP70.
[15]"LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway."
Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.
Nat. Commun. 2:277-277(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LTD.
[16]"Protein import into chloroplasts: the Tic complex and its regulation."
Kovacs-Bogdan E., Soll J., Bolter B.
Biochim. Biophys. Acta 1803:740-747(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC011001 Genomic DNA. Translation: AAF63131.1.
AC067971 Genomic DNA. Translation: AAF82224.1.
CP002684 Genomic DNA. Translation: AEE28057.1.
AY094426 mRNA. Translation: AAM19799.1.
AY099850 mRNA. Translation: AAM20701.1.
AK229869 mRNA. Translation: BAF01698.1.
PIRB86204.
RefSeqNP_172176.1. NM_100568.3.
UniGeneAt.12081.

3D structure databases

ProteinModelPortalQ8LPR9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22446. 9 interactions.
IntActQ8LPR9. 7 interactions.
STRING3702.AT1G06950.1-P.

Protein family/group databases

TCDB3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

Proteomic databases

PRIDEQ8LPR9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G06950.1; AT1G06950.1; AT1G06950.
GeneID837205.
KEGGath:AT1G06950.

Organism-specific databases

TAIRAT1G06950.

Phylogenomic databases

eggNOGNOG259340.
HOGENOMHOG000243512.
InParanoidQ8LPR9.
OMAHMEIGRR.
PhylomeDBQ8LPR9.
ProtClustDBCLSN2682158.

Gene expression databases

GenevestigatorQ8LPR9.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameTI110_ARATH
AccessionPrimary (citable) accession number: Q8LPR9
Secondary accession number(s): Q0WMF8 expand/collapse secondary AC list , Q8LPG5, Q9LMI9, Q9M9Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names