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Q8LPR9

- TI110_ARATH

UniProt

Q8LPR9 - TI110_ARATH

Protein

Protein TIC110, chloroplastic

Gene

TIC110

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Involved in protein precursor import into chloroplasts. Forms a voltage-dependent cation-selective channel at the inner envelope of chloroplasts, which specifically responds to a transit peptide. Associates with both the precursor and mature forms of the preprotein.4 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. chloroplast organization Source: TAIR
    2. protein import into chloroplast stroma Source: TAIR

    Keywords - Biological processi

    Protein transport, Transport

    Protein family/group databases

    TCDBi3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein TIC110, chloroplastic
    Alternative name(s):
    Translocon at the inner envelope membrane of chloroplasts 110
    Short name:
    AtTIC110
    Gene namesi
    Name:TIC110
    Ordered Locus Names:At1g06950
    ORF Names:F10K1.33, F4H5.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G06950.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: TAIR
    5. plastid Source: TAIR
    6. Tic complex Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Embryo lethal.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050ChloroplastSequence AnalysisAdd
    BLAST
    Chaini51 – 1016966Protein TIC110, chloroplasticPRO_0000413668Add
    BLAST

    Proteomic databases

    PRIDEiQ8LPR9.

    Expressioni

    Tissue specificityi

    Expressed in seedlings, flowers, leaves, stems and roots.3 Publications

    Developmental stagei

    Expressed throughout development.1 Publication

    Gene expression databases

    GenevestigatoriQ8LPR9.

    Interactioni

    Subunit structurei

    Part of the Tic complex. Interacts with HSP70, HSP93 and TIC40. Interacts with the Toc complex components TOC33, TOC75 and TOC159. Interacts with LTD.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIC40Q9FMD53EBI-639092,EBI-639157

    Protein-protein interaction databases

    BioGridi22446. 9 interactions.
    IntActiQ8LPR9. 7 interactions.
    STRINGi3702.AT1G06950.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LPR9.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini51 – 9545StromalBy similarityAdd
    BLAST
    Topological domaini117 – 1182Chloroplast intermembraneBy similarity
    Topological domaini140 – 259120StromalBy similarityAdd
    BLAST
    Topological domaini281 – 36888Chloroplast intermembraneBy similarityAdd
    BLAST
    Topological domaini387 – 632246StromalBy similarityAdd
    BLAST
    Topological domaini651 – 71969Chloroplast intermembraneBy similarityAdd
    BLAST
    Topological domaini737 – 1016280StromalBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei96 – 11621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei119 – 13921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei369 – 38618HelicalBy similarityAdd
    BLAST
    Transmembranei633 – 65018HelicalBy similarityAdd
    BLAST
    Transmembranei720 – 73617HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Domaini

    An internal domain (143-652) is sufficient for assembly into supercomplex with Toc complex.
    Residues 235-420 contain a binding site for preprotein transit peptides.

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259340.
    HOGENOMiHOG000243512.
    InParanoidiQ8LPR9.
    OMAiHMEIGRR.
    PhylomeDBiQ8LPR9.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LPR9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPSLVTAIN APISPSPRSP LLSHFLPTLP HRFSKSECLS RRRYRVSFPR     50
    SSAASSDQLS VSTQAKNPGI HGNKKELTGL QPIVEKMTPP VRLATSAVVL 100
    AASLATGYGL GLRLAGSRNI AFGGAAVAGA AGGAVVYALN SAVPEVAAIS 150
    LHNYVAEFED PASVTKDDVE KIADRYGVNK GDEAFQAEIC DIYCRYVTSV 200
    LPTEGQSLKG DEVAKIVKFK NALGIDEPDA AAMHMEIGRR IFRQRLETGE 250
    REGDAEQRRA FMRLVYVSAL VFGDASSFLL PWKRVLKVTD AQVEIAIREN 300
    AKQLYAERLK LVGRDINVEN LVDLRKSQLS FKLSDELAED LFREHTRKVV 350
    VENISSALSI LKSRTRAAKS LASVVEELEK VLEFNNLLVS LKSHSEADQF 400
    ARGVGPISLI GDESDFERRM DDLKLLYRAY VTDALSGGRL EENKLVAMSQ 450
    LRNILGLGKR EAEAISVDVT SKSYRKRLAN AVSSGDLEAQ DSKAKYLQKL 500
    CEELHFDAQK AGAIHEEIYR QKLQQCVTDG ELSDDNVAAL LRLRVMLCIP 550
    QQTVDTAHAE ICGTIFEKVV RDAISSGVDG YDAETRKSVR KAAHGLRLSR 600
    ETAMSIASKA VRRVFTNYIR RARAAENRTD SAKELKKMIA FNTLVVTEMV 650
    ADIKGESSDK APEEDPVQEK EEDDEDEEWG SLESLRKTRP DKELAEKMGK 700
    PGQTEITLKD DLPDRDRIDL YKTYLLYCVT GEVTRIPFGA QITTKRDDSE 750
    YLLLNQLGGI LGLSSKEIVN IHVGLAEQAF RQQAEVILAD GQLTKARVEQ 800
    LDELQKQVGL PQPQAEKVIK NITTTKMANA IETAVNQGRL NIKQIRELKE 850
    ANVSLDSMIA VSLREKLFKK TVSDIFSSGT GEFDETEVYQ TIPSDLSIDV 900
    EKAKRVVHDL AQSRLSNSLV QAVALLRQRN SKGVVLSLND LLACDKAVPA 950
    EPMSWEVSEE LSDLYAIYSK SDPKPAPEKV LRLQYLLGID DSTATALREM 1000
    EDGALSSAAE EGNFVF 1016
    Length:1,016
    Mass (Da):112,121
    Last modified:October 1, 2002 - v1
    Checksum:i814CCDE07EB5D0EA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti220 – 2201K → N in AAM20701. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC011001 Genomic DNA. Translation: AAF63131.1.
    AC067971 Genomic DNA. Translation: AAF82224.1.
    CP002684 Genomic DNA. Translation: AEE28057.1.
    AY094426 mRNA. Translation: AAM19799.1.
    AY099850 mRNA. Translation: AAM20701.1.
    AK229869 mRNA. Translation: BAF01698.1.
    PIRiB86204.
    RefSeqiNP_172176.1. NM_100568.3.
    UniGeneiAt.12081.

    Genome annotation databases

    EnsemblPlantsiAT1G06950.1; AT1G06950.1; AT1G06950.
    GeneIDi837205.
    KEGGiath:AT1G06950.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC011001 Genomic DNA. Translation: AAF63131.1 .
    AC067971 Genomic DNA. Translation: AAF82224.1 .
    CP002684 Genomic DNA. Translation: AEE28057.1 .
    AY094426 mRNA. Translation: AAM19799.1 .
    AY099850 mRNA. Translation: AAM20701.1 .
    AK229869 mRNA. Translation: BAF01698.1 .
    PIRi B86204.
    RefSeqi NP_172176.1. NM_100568.3.
    UniGenei At.12081.

    3D structure databases

    ProteinModelPortali Q8LPR9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 22446. 9 interactions.
    IntActi Q8LPR9. 7 interactions.
    STRINGi 3702.AT1G06950.1-P.

    Protein family/group databases

    TCDBi 3.A.9.1.2. the chloroplast envelope protein translocase (cept or tic-toc) family.

    Proteomic databases

    PRIDEi Q8LPR9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G06950.1 ; AT1G06950.1 ; AT1G06950 .
    GeneIDi 837205.
    KEGGi ath:AT1G06950.

    Organism-specific databases

    TAIRi AT1G06950.

    Phylogenomic databases

    eggNOGi NOG259340.
    HOGENOMi HOG000243512.
    InParanoidi Q8LPR9.
    OMAi HMEIGRR.
    PhylomeDBi Q8LPR9.

    Gene expression databases

    Genevestigatori Q8LPR9.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 823-1016.
      Strain: cv. Columbia.
    5. "The hydrophilic domain of Tic110, an inner envelope membrane component of the chloroplastic protein translocation apparatus, faces the stromal compartment."
      Jackson D.T., Froehlich J.E., Keegstra K.
      J. Biol. Chem. 273:16583-16588(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    6. "The preprotein conducting channel at the inner envelope membrane of plastids."
      Heins L., Mehrle A., Hemmler R., Wagner R., Kuechler M., Hoermann F., Sveshnikov D., Soll J.
      EMBO J. 21:2616-2625(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "atTic110 functions as a scaffold for coordinating the stromal events of protein import into chloroplasts."
      Inaba T., Li M., Alvarez-Huerta M., Kessler F., Schnell D.J.
      J. Biol. Chem. 278:38617-38627(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TOPOLOGY.
    8. Cited for: TISSUE SPECIFICITY.
    9. "Arabidopsis tic110 is essential for the assembly and function of the protein import machinery of plastids."
      Inaba T., Alvarez-Huerta M., Li M., Bauer J., Ewers C., Kessler F., Schnell D.J.
      Plant Cell 17:1482-1496(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH HSP93; TIC40 AND TOC COMPLEX.
    10. "In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast protein import."
      Kovacheva S., Bedard J., Patel R., Dudley P., Twell D., Rios G., Koncz C., Jarvis P.
      Plant J. 41:412-428(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    11. "Reconstitution of protein targeting to the inner envelope membrane of chloroplasts."
      Li M., Schnell D.J.
      J. Cell Biol. 175:249-259(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    12. "Precursor binding to an 880-kDa Toc complex as an early step during active import of protein into chloroplasts."
      Chen K.Y., Li H.M.
      Plant J. 49:149-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN TIC/TOC SUPERCOMPLEX.
    13. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts."
      Su P.H., Li H.M.
      Plant Cell 22:1516-1531(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HSP70.
    15. "LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway."
      Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.
      Nat. Commun. 2:277-277(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LTD.
    16. "Protein import into chloroplasts: the Tic complex and its regulation."
      Kovacs-Bogdan E., Soll J., Bolter B.
      Biochim. Biophys. Acta 1803:740-747(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiTI110_ARATH
    AccessioniPrimary (citable) accession number: Q8LPR9
    Secondary accession number(s): Q0WMF8
    , Q8LPG5, Q9LMI9, Q9M9Z7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The region 143-1016 is indicated to be located in the stroma (PubMed:12874276, PubMed:9632730) while the homologous region in pea TIC110 contains probably 4 trans-membrane domains resulting in 2 regions in the intermembrane space localized to form supercomplexes with the TOC machinery and to receive the transit peptide of preproteins.2 Publications
    Inserts into the inner envelope membrane from the stroma after import from the cytoplasm.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3