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Protein

Cellulose synthase A catalytic subunit 8 [UDP-forming]

Gene

CESA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the secondary cell wall formation. Required for the xylem cell wall thickening.4 Publications

Catalytic activityi

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: plant cellulose biosynthesis

This protein is involved in the pathway plant cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway plant cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Zinc 1By similarity1
Metal bindingi12Zinc 1By similarity1
Metal bindingi28Zinc 2By similarity1
Metal bindingi31Zinc 2By similarity1
Metal bindingi36Zinc 1By similarity1
Metal bindingi39Zinc 1By similarity1
Metal bindingi51Zinc 2By similarity1
Metal bindingi54Zinc 2By similarity1
Active sitei304Sequence analysis1
Binding sitei470SubstrateSequence analysis1
Binding sitei472SubstrateSequence analysis1
Active sitei683Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri9 – 55RING-type; degenerateAdd BLAST47

GO - Molecular functioni

  • cellulose synthase (UDP-forming) activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellulose biosynthetic process Source: TAIR
  • cell wall organization Source: UniProtKB-KW
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • plant-type secondary cell wall biogenesis Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation, Cellulose biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2366.
BRENDAi2.4.1.12. 399.
UniPathwayiUPA00695.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.
TCDBi4.D.3.1.7. the glycan glucosyl transferase (opgh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose synthase A catalytic subunit 8 [UDP-forming] (EC:2.4.1.12)
Short name:
AtCesA8
Alternative name(s):
Protein IRREGULAR XYLEM 1
Short name:
AtIRX1
Protein LEAF WILTING 2
Gene namesi
Name:CESA8
Synonyms:IRX1, LEW2
Ordered Locus Names:At4g18780
ORF Names:F28A21.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G18780.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 179CytoplasmicSequence analysisAdd BLAST179
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21
Topological domaini201 – 208ExtracellularSequence analysis8
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Topological domaini230 – 767CytoplasmicSequence analysisAdd BLAST538
Transmembranei768 – 788HelicalSequence analysisAdd BLAST21
Topological domaini789 – 793ExtracellularSequence analysis5
Transmembranei794 – 814HelicalSequence analysisAdd BLAST21
Topological domaini815 – 829CytoplasmicSequence analysisAdd BLAST15
Transmembranei830 – 850HelicalSequence analysisAdd BLAST21
Topological domaini851 – 879ExtracellularSequence analysisAdd BLAST29
Transmembranei880 – 900HelicalSequence analysisAdd BLAST21
Topological domaini901 – 911CytoplasmicSequence analysisAdd BLAST11
Transmembranei912 – 932HelicalSequence analysisAdd BLAST21
Topological domaini933 – 941ExtracellularSequence analysis9
Transmembranei942 – 962HelicalSequence analysisAdd BLAST21
Topological domaini963 – 985CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

  • cell wall Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Enhanced resistance to the pathogens Ralstonia solanacearum and Plectosphaerella cucumerina.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi679S → L in irx1-2; reduced levels of crystalline cellulose in secondary cell wall. 2 Publications1
Mutagenesisi683D → N in irx1-1; reduced levels of crystalline cellulose in secondary cell wall. 2 Publications1
Mutagenesisi802L → F in lew2-2; enhanced tolerance to drought, salt and osmotic stresses. Higher accumulation of abscisic acid (ABA), proline and soluble sugars. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001663741 – 985Cellulose synthase A catalytic subunit 8 [UDP-forming]Add BLAST985

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi857N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

S-acylated.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ8LPK5.

Expressioni

Tissue specificityi

Confined to secondary cell wall developing tissues such as xylems and interfascicular regions. Expressed in young plants, stems, flowers and inflorescences, but not in leaves.3 Publications

Developmental stagei

Not found in embryos. Increasing amount as stems mature.1 Publication

Gene expression databases

ExpressionAtlasiQ8LPK5. baseline and differential.
GenevisibleiQ8LPK5. AT.

Interactioni

Subunit structurei

Interacts with CESA4 and CESA7. Assembly with CESA4 and CESA7 is required for functional complex and localization in secondary cell wall deposition sites. Interacts with STL1 and STL2, but not with GOT1 (PubMed:27277162).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CESA4Q84JA64EBI-8579199,EBI-8579072
CESA7Q9SWW67EBI-8579199,EBI-4477361

Protein-protein interaction databases

BioGridi12905. 17 interactors.
IntActiQ8LPK5. 2 interactors.
MINTiMINT-6950961.
STRINGi3702.AT4G18780.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LPK5.
SMRiQ8LPK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili358 – 385Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 140Lys-richAdd BLAST19
Compositional biasi565 – 569Poly-Ser5

Sequence similaritiesi

Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri9 – 55RING-type; degenerateAdd BLAST47

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGV4. Eukaryota.
COG1215. LUCA.
HOGENOMiHOG000241942.
InParanoidiQ8LPK5.
KOiK10999.
OMAiWCGCCSC.
OrthoDBiEOG0936016G.
PhylomeDBiQ8LPK5.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR005150. Cellulose_synth.
IPR027934. CES_Znf_RING.
IPR029044. Nucleotide-diphossugar_trans.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF14569. zf-UDP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

Q8LPK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMESRSPICN TCGEEIGVKS NGEFFVACHE CSFPICKACL EYEFKEGRRI
60 70 80 90 100
CLRCGNPYDE NVFDDVETKT SKTQSIVPTQ TNNTSQDSGI HARHISTVST
110 120 130 140 150
IDSELNDEYG NPIWKNRVES WKDKKDKKSK KKKKDPKATK AEQHEAQIPT
160 170 180 190 200
QQHMEDTPPN TESGATDVLS VVIPIPRTKI TSYRIVIIMR LIILALFFNY
210 220 230 240 250
RITHPVDSAY GLWLTSVICE IWFAVSWVLD QFPKWSPINR ETYIDRLSAR
260 270 280 290 300
FEREGEQSQL AAVDFFVSTV DPLKEPPLIT ANTVLSILAL DYPVDKVSCY
310 320 330 340 350
VSDDGAAMLS FESLVETADF ARKWVPFCKK YSIEPRAPEF YFSLKIDYLR
360 370 380 390 400
DKVQPSFVKE RRAMKRDYEE FKIRMNALVA KAQKTPEEGW TMQDGTSWPG
410 420 430 440 450
NNTRDHPGMI QVFLGYSGAR DIEGNELPRL VYVSREKRPG YQHHKKAGAE
460 470 480 490 500
NALVRVSAVL TNAPFILNLD CDHYVNNSKA VREAMCFLMD PVVGQDVCFV
510 520 530 540 550
QFPQRFDGID KSDRYANRNI VFFDVNMRGL DGIQGPVYVG TGTVFRRQAL
560 570 580 590 600
YGYSPPSKPR ILPQSSSSSC CCLTKKKQPQ DPSEIYKDAK REELDAAIFN
610 620 630 640 650
LGDLDNYDEY DRSMLISQTS FEKTFGLSTV FIESTLMENG GVPDSVNPST
660 670 680 690 700
LIKEAIHVIS CGYEEKTEWG KEIGWIYGSI TEDILTGFKM HCRGWRSIYC
710 720 730 740 750
MPLRPAFKGS APINLSDRLH QVLRWALGSV EIFLSRHCPL WYGCSGGRLK
760 770 780 790 800
LLQRLAYINT IVYPFTSLPL VAYCTLPAIC LLTGKFIIPT LSNLASMLFL
810 820 830 840 850
GLFISIILTS VLELRWSGVS IEDLWRNEQF WVIGGVSAHL FAVFQGFLKM
860 870 880 890 900
LAGLDTNFTV TSKTADDLEF GELYIVKWTT LLIPPTSLLI INLVGVVAGF
910 920 930 940 950
SDALNKGYEA WGPLFGKVFF AFWVILHLYP FLKGLMGRQN RTPTIVILWS
960 970 980
ILLASVFSLV WVRINPFVSK TDTTSLSLNC LLIDC
Length:985
Mass (Da):111,521
Last modified:October 1, 2002 - v1
Checksum:iD299F357625768E0
GO

Sequence cautioni

The sequence CAB37463 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78880 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18V → A in AAK08700 (PubMed:11148295).Curated1
Sequence conflicti144 – 145HE → QD in AAK08700 (PubMed:11148295).Curated2
Sequence conflicti148I → V in AAK08700 (PubMed:11148295).Curated1
Sequence conflicti157T → M in AAK08700 (PubMed:11148295).Curated1
Sequence conflicti583S → A in AAK08700 (PubMed:11148295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF267742 mRNA. Translation: AAK08700.1.
AL035526 Genomic DNA. Translation: CAB37463.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78880.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84089.1.
AY099636 mRNA. Translation: AAM20487.1.
PIRiT04870.
RefSeqiNP_567564.1. NM_117994.4.
UniGeneiAt.66621.
At.68239.

Genome annotation databases

EnsemblPlantsiAT4G18780.1; AT4G18780.1; AT4G18780.
GeneIDi827612.
GrameneiAT4G18780.1; AT4G18780.1; AT4G18780.
KEGGiath:AT4G18780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF267742 mRNA. Translation: AAK08700.1.
AL035526 Genomic DNA. Translation: CAB37463.1. Sequence problems.
AL161549 Genomic DNA. Translation: CAB78880.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84089.1.
AY099636 mRNA. Translation: AAM20487.1.
PIRiT04870.
RefSeqiNP_567564.1. NM_117994.4.
UniGeneiAt.66621.
At.68239.

3D structure databases

ProteinModelPortaliQ8LPK5.
SMRiQ8LPK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12905. 17 interactors.
IntActiQ8LPK5. 2 interactors.
MINTiMINT-6950961.
STRINGi3702.AT4G18780.1.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.
TCDBi4.D.3.1.7. the glycan glucosyl transferase (opgh) family.

Proteomic databases

PaxDbiQ8LPK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G18780.1; AT4G18780.1; AT4G18780.
GeneIDi827612.
GrameneiAT4G18780.1; AT4G18780.1; AT4G18780.
KEGGiath:AT4G18780.

Organism-specific databases

TAIRiAT4G18780.

Phylogenomic databases

eggNOGiENOG410IGV4. Eukaryota.
COG1215. LUCA.
HOGENOMiHOG000241942.
InParanoidiQ8LPK5.
KOiK10999.
OMAiWCGCCSC.
OrthoDBiEOG0936016G.
PhylomeDBiQ8LPK5.

Enzyme and pathway databases

UniPathwayiUPA00695.
BioCyciMetaCyc:MONOMER-2366.
BRENDAi2.4.1.12. 399.

Miscellaneous databases

PROiQ8LPK5.

Gene expression databases

ExpressionAtlasiQ8LPK5. baseline and differential.
GenevisibleiQ8LPK5. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR005150. Cellulose_synth.
IPR027934. CES_Znf_RING.
IPR029044. Nucleotide-diphossugar_trans.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF14569. zf-UDP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCESA8_ARATH
AccessioniPrimary (citable) accession number: Q8LPK5
Secondary accession number(s): Q9C5Z8, Q9SN37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.