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Protein

Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial

Gene

At5g14590

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in response to oxidative stresses.By similarity

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149SubstrateBy similarity1
Binding sitei154NADPBy similarity1
Binding sitei181SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Sitei211Critical for catalysisBy similarity1
Sitei283Critical for catalysisBy similarity1
Metal bindingi323Magnesium or manganeseBy similarity1
Binding sitei331NADPBy similarity1
Metal bindingi346Magnesium or manganeseBy similarity1
Binding sitei399NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi147 – 149NADPBy similarity3
Nucleotide bindingi381 – 386NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response, Tricarboxylic acid cycle
LigandMagnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G14590-MONOMER
ReactomeiR-ATH-389542 NADPH regeneration
R-ATH-6798695 Neutrophil degranulation
R-ATH-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial (EC:1.1.1.42)
Gene namesi
Ordered Locus Names:At5g14590
ORF Names:T15N1.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G14590
TAIRilocus:2222672 AT5G14590

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65Chloroplast and mitochondrionSequence analysisAdd BLAST65
ChainiPRO_000042196466 – 485Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrialAdd BLAST420

Proteomic databases

PaxDbiQ8LPJ5
PRIDEiQ8LPJ5

Expressioni

Gene expression databases

ExpressionAtlasiQ8LPJ5 baseline and differential
GenevisibleiQ8LPJ5 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G14590.1

Structurei

3D structure databases

ProteinModelPortaliQ8LPJ5
SMRiQ8LPJ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 172Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1526 Eukaryota
COG0538 LUCA
HOGENOMiHOG000019858
InParanoidiQ8LPJ5
KOiK00031
OMAiAMGMYNQ
OrthoDBiEOG09360AQU
PhylomeDBiQ8LPJ5

Family and domain databases

InterProiView protein in InterPro
IPR019818 IsoCit/isopropylmalate_DH_CS
IPR004790 Isocitrate_DH_NADP
IPR024084 IsoPropMal-DH-like_dom
PANTHERiPTHR11822 PTHR11822, 1 hit
PfamiView protein in Pfam
PF00180 Iso_dh, 1 hit
PIRSFiPIRSF000108 IDH_NADP, 1 hit
SMARTiView protein in SMART
SM01329 Iso_dh, 1 hit
TIGRFAMsiTIGR00127 nadp_idh_euk, 1 hit
PROSITEiView protein in PROSITE
PS00470 IDH_IMDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LPJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKLTHGVF TYRASLTAML SSSTSAGLSS SFVSSRFLSS GIFSSGASRN
60 70 80 90 100
RVTFPVQFHR ASAVRCFASS GGSDRIQVQN PIVEMDGDEM TRVIWSMIKE
110 120 130 140 150
KLILPYLDLD IKYFDLGILN RDATDDKVTV ESAEAALKYN VAIKCATITP
160 170 180 190 200
DEGRVKEFGL KSMWRSPNGT IRNILDGTVF REPIMCSNIP RLVPGWEKPI
210 220 230 240 250
CIGRHAFGDQ YRATDTVIKG PGKLKMVFVP EDGNAPVELD VYDFKGPGVA
260 270 280 290 300
LAMYNVDESI RAFAESSMAM ALTKKWPLYL STKNTILKKY DGRFKDIFQE
310 320 330 340 350
VYEANWKQKF EEHSIWYEHR LIDDMVAYAV KSEGGYVWAC KNYDGDVQSD
360 370 380 390 400
LLAQGFGSLG LMTSVLLSAD GKTLESEAAH GTVTRHFRLH QKGQETSTNS
410 420 430 440 450
IASIFAWTRG LEHRAKLDKN EKLMDFVKKL ESSCVNTVET GKMTKDLALL
460 470 480
IHGPKVSRDL FLNTEEFIDA VASKLKTQFK ELPLV
Length:485
Mass (Da):54,196
Last modified:October 1, 2002 - v1
Checksum:iCBCD72BB38C7EF5F
GO

Sequence cautioni

The sequence CAB87626 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163792 Genomic DNA Translation: CAB87626.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92051.1
AY099683 mRNA Translation: AAM20534.1
BT000276 mRNA Translation: AAN15595.1
PIRiT48632
RefSeqiNP_196963.2, NM_121463.5
UniGeneiAt.46811

Genome annotation databases

EnsemblPlantsiAT5G14590.1; AT5G14590.1; AT5G14590
GeneIDi831311
GrameneiAT5G14590.1; AT5G14590.1; AT5G14590
KEGGiath:AT5G14590

Similar proteinsi

Entry informationi

Entry nameiICDHP_ARATH
AccessioniPrimary (citable) accession number: Q8LPJ5
Secondary accession number(s): Q9LYK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health