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Q8LPJ5

- ICDHP_ARATH

UniProt

Q8LPJ5 - ICDHP_ARATH

Protein

Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial

Gene

At5g14590

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    May be involved in response to oxidative stresses.By similarity

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei154 – 1541NADPBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Sitei211 – 2111Critical for catalysisBy similarity
    Sitei283 – 2831Critical for catalysisBy similarity
    Metal bindingi323 – 3231Magnesium or manganeseBy similarity
    Binding sitei331 – 3311NADPBy similarity
    Metal bindingi346 – 3461Magnesium or manganeseBy similarity
    Binding sitei399 – 3991NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi147 – 1493NADPBy similarity
    Nucleotide bindingi381 – 3866NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. response to stress Source: UniProtKB-KW
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Stress response, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT5G14590-MONOMER.
    ReactomeiREACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_218202. NADPH regeneration.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial (EC:1.1.1.42)
    Gene namesi
    Ordered Locus Names:At5g14590
    ORF Names:T15N1.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G14590.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR
    3. chloroplast thylakoid Source: TAIR
    4. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Mitochondrion, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6565Chloroplast and mitochondrionSequence AnalysisAdd
    BLAST
    Chaini66 – 485420Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrialPRO_0000421964Add
    BLAST

    Proteomic databases

    PaxDbiQ8LPJ5.
    PRIDEiQ8LPJ5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8LPJ5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G14590.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LPJ5.
    SMRiQ8LPJ5. Positions 75-475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1727Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0538.
    HOGENOMiHOG000019858.
    InParanoidiQ8LPJ5.
    KOiK00031.
    OMAiCVNTVET.
    PhylomeDBiQ8LPJ5.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LPJ5-1 [UniParc]FASTAAdd to Basket

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    MLNKLTHGVF TYRASLTAML SSSTSAGLSS SFVSSRFLSS GIFSSGASRN    50
    RVTFPVQFHR ASAVRCFASS GGSDRIQVQN PIVEMDGDEM TRVIWSMIKE 100
    KLILPYLDLD IKYFDLGILN RDATDDKVTV ESAEAALKYN VAIKCATITP 150
    DEGRVKEFGL KSMWRSPNGT IRNILDGTVF REPIMCSNIP RLVPGWEKPI 200
    CIGRHAFGDQ YRATDTVIKG PGKLKMVFVP EDGNAPVELD VYDFKGPGVA 250
    LAMYNVDESI RAFAESSMAM ALTKKWPLYL STKNTILKKY DGRFKDIFQE 300
    VYEANWKQKF EEHSIWYEHR LIDDMVAYAV KSEGGYVWAC KNYDGDVQSD 350
    LLAQGFGSLG LMTSVLLSAD GKTLESEAAH GTVTRHFRLH QKGQETSTNS 400
    IASIFAWTRG LEHRAKLDKN EKLMDFVKKL ESSCVNTVET GKMTKDLALL 450
    IHGPKVSRDL FLNTEEFIDA VASKLKTQFK ELPLV 485
    Length:485
    Mass (Da):54,196
    Last modified:October 1, 2002 - v1
    Checksum:iCBCD72BB38C7EF5F
    GO

    Sequence cautioni

    The sequence CAB87626.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163792 Genomic DNA. Translation: CAB87626.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92051.1.
    AY099683 mRNA. Translation: AAM20534.1.
    BT000276 mRNA. Translation: AAN15595.1.
    PIRiT48632.
    RefSeqiNP_196963.2. NM_121463.4.
    UniGeneiAt.46811.

    Genome annotation databases

    EnsemblPlantsiAT5G14590.1; AT5G14590.1; AT5G14590.
    GeneIDi831311.
    KEGGiath:AT5G14590.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163792 Genomic DNA. Translation: CAB87626.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92051.1 .
    AY099683 mRNA. Translation: AAM20534.1 .
    BT000276 mRNA. Translation: AAN15595.1 .
    PIRi T48632.
    RefSeqi NP_196963.2. NM_121463.4.
    UniGenei At.46811.

    3D structure databases

    ProteinModelPortali Q8LPJ5.
    SMRi Q8LPJ5. Positions 75-475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G14590.1-P.

    Proteomic databases

    PaxDbi Q8LPJ5.
    PRIDEi Q8LPJ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G14590.1 ; AT5G14590.1 ; AT5G14590 .
    GeneIDi 831311.
    KEGGi ath:AT5G14590.

    Organism-specific databases

    GeneFarmi 4411.
    TAIRi AT5G14590.

    Phylogenomic databases

    eggNOGi COG0538.
    HOGENOMi HOG000019858.
    InParanoidi Q8LPJ5.
    KOi K00031.
    OMAi CVNTVET.
    PhylomeDBi Q8LPJ5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G14590-MONOMER.
    Reactomei REACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
    REACT_218202. NADPH regeneration.

    Miscellaneous databases

    PROi Q8LPJ5.

    Gene expression databases

    Genevestigatori Q8LPJ5.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "Defining the protein complex proteome of plant mitochondria."
      Klodmann J., Senkler M., Rode C., Braun H.-P.
      Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiICDHP_ARATH
    AccessioniPrimary (citable) accession number: Q8LPJ5
    Secondary accession number(s): Q9LYK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3