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Q8LPJ5 (ICDHP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial

EC=1.1.1.42
Gene names
Ordered Locus Names:At5g14590
ORF Names:T15N1.80
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in response to oxidative stresses By similarity.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subcellular location

Plastidchloroplast. Mitochondrion Ref.4 Ref.5 Ref.6 Ref.7.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence caution

The sequence CAB87626.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6565Chloroplast and mitochondrion Potential
Chain66 – 485420Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial
PRO_0000421964

Regions

Nucleotide binding147 – 1493NADP By similarity
Nucleotide binding381 – 3866NADP By similarity
Region166 – 1727Substrate binding By similarity

Sites

Metal binding3231Magnesium or manganese By similarity
Metal binding3461Magnesium or manganese By similarity
Binding site1491Substrate By similarity
Binding site1541NADP By similarity
Binding site1811Substrate By similarity
Binding site2041Substrate By similarity
Binding site3311NADP By similarity
Binding site3991NADP; via amide nitrogen and carbonyl oxygen By similarity
Site2111Critical for catalysis By similarity
Site2831Critical for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8LPJ5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: CBCD72BB38C7EF5F

FASTA48554,196
        10         20         30         40         50         60 
MLNKLTHGVF TYRASLTAML SSSTSAGLSS SFVSSRFLSS GIFSSGASRN RVTFPVQFHR 

        70         80         90        100        110        120 
ASAVRCFASS GGSDRIQVQN PIVEMDGDEM TRVIWSMIKE KLILPYLDLD IKYFDLGILN 

       130        140        150        160        170        180 
RDATDDKVTV ESAEAALKYN VAIKCATITP DEGRVKEFGL KSMWRSPNGT IRNILDGTVF 

       190        200        210        220        230        240 
REPIMCSNIP RLVPGWEKPI CIGRHAFGDQ YRATDTVIKG PGKLKMVFVP EDGNAPVELD 

       250        260        270        280        290        300 
VYDFKGPGVA LAMYNVDESI RAFAESSMAM ALTKKWPLYL STKNTILKKY DGRFKDIFQE 

       310        320        330        340        350        360 
VYEANWKQKF EEHSIWYEHR LIDDMVAYAV KSEGGYVWAC KNYDGDVQSD LLAQGFGSLG 

       370        380        390        400        410        420 
LMTSVLLSAD GKTLESEAAH GTVTRHFRLH QKGQETSTNS IASIFAWTRG LEHRAKLDKN 

       430        440        450        460        470        480 
EKLMDFVKKL ESSCVNTVET GKMTKDLALL IHGPKVSRDL FLNTEEFIDA VASKLKTQFK 


ELPLV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Defining the protein complex proteome of plant mitochondria."
Klodmann J., Senkler M., Rode C., Braun H.-P.
Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL163792 Genomic DNA. Translation: CAB87626.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92051.1.
AY099683 mRNA. Translation: AAM20534.1.
BT000276 mRNA. Translation: AAN15595.1.
PIRT48632.
RefSeqNP_196963.2. NM_121463.4.
UniGeneAt.46811.

3D structure databases

ProteinModelPortalQ8LPJ5.
SMRQ8LPJ5. Positions 75-475.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G14590.1-P.

Proteomic databases

PaxDbQ8LPJ5.
PRIDEQ8LPJ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G14590.1; AT5G14590.1; AT5G14590.
GeneID831311.
KEGGath:AT5G14590.

Organism-specific databases

GeneFarm4411.
TAIRAT5G14590.

Phylogenomic databases

eggNOGCOG0538.
HOGENOMHOG000019858.
InParanoidQ8LPJ5.
KOK00031.
OMACVNTVET.
PhylomeDBQ8LPJ5.

Enzyme and pathway databases

BioCycARA:AT5G14590-MONOMER.

Gene expression databases

GenevestigatorQ8LPJ5.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8LPJ5.

Entry information

Entry nameICDHP_ARATH
AccessionPrimary (citable) accession number: Q8LPJ5
Secondary accession number(s): Q9LYK1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names