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Q8LPJ5

- ICDHP_ARATH

UniProt

Q8LPJ5 - ICDHP_ARATH

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Protein

Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial

Gene
At5g14590, T15N1.80
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

May be involved in response to oxidative stresses By similarity.

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491Substrate By similarity
Binding sitei154 – 1541NADP By similarity
Binding sitei181 – 1811Substrate By similarity
Binding sitei204 – 2041Substrate By similarity
Sitei211 – 2111Critical for catalysis By similarity
Sitei283 – 2831Critical for catalysis By similarity
Metal bindingi323 – 3231Magnesium or manganese By similarity
Binding sitei331 – 3311NADP By similarity
Metal bindingi346 – 3461Magnesium or manganese By similarity
Binding sitei399 – 3991NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi147 – 1493NADP By similarity
Nucleotide bindingi381 – 3866NADP By similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. response to stress Source: UniProtKB-KW
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT5G14590-MONOMER.
ReactomeiREACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
REACT_218202. NADPH regeneration.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrial (EC:1.1.1.42)
Gene namesi
Ordered Locus Names:At5g14590
ORF Names:T15N1.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G14590.

Subcellular locationi

Plastidchloroplast. Mitochondrion 4 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. chloroplast thylakoid Source: TAIR
  4. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6565Chloroplast and mitochondrion Reviewed predictionAdd
BLAST
Chaini66 – 485420Isocitrate dehydrogenase [NADP], chloroplastic/mitochondrialPRO_0000421964Add
BLAST

Proteomic databases

PaxDbiQ8LPJ5.
PRIDEiQ8LPJ5.

Expressioni

Gene expression databases

GenevestigatoriQ8LPJ5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G14590.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LPJ5.
SMRiQ8LPJ5. Positions 75-475.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 1727Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
InParanoidiQ8LPJ5.
KOiK00031.
OMAiCVNTVET.
PhylomeDBiQ8LPJ5.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LPJ5-1 [UniParc]FASTAAdd to Basket

« Hide

MLNKLTHGVF TYRASLTAML SSSTSAGLSS SFVSSRFLSS GIFSSGASRN    50
RVTFPVQFHR ASAVRCFASS GGSDRIQVQN PIVEMDGDEM TRVIWSMIKE 100
KLILPYLDLD IKYFDLGILN RDATDDKVTV ESAEAALKYN VAIKCATITP 150
DEGRVKEFGL KSMWRSPNGT IRNILDGTVF REPIMCSNIP RLVPGWEKPI 200
CIGRHAFGDQ YRATDTVIKG PGKLKMVFVP EDGNAPVELD VYDFKGPGVA 250
LAMYNVDESI RAFAESSMAM ALTKKWPLYL STKNTILKKY DGRFKDIFQE 300
VYEANWKQKF EEHSIWYEHR LIDDMVAYAV KSEGGYVWAC KNYDGDVQSD 350
LLAQGFGSLG LMTSVLLSAD GKTLESEAAH GTVTRHFRLH QKGQETSTNS 400
IASIFAWTRG LEHRAKLDKN EKLMDFVKKL ESSCVNTVET GKMTKDLALL 450
IHGPKVSRDL FLNTEEFIDA VASKLKTQFK ELPLV 485
Length:485
Mass (Da):54,196
Last modified:October 1, 2002 - v1
Checksum:iCBCD72BB38C7EF5F
GO

Sequence cautioni

The sequence CAB87626.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL163792 Genomic DNA. Translation: CAB87626.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92051.1.
AY099683 mRNA. Translation: AAM20534.1.
BT000276 mRNA. Translation: AAN15595.1.
PIRiT48632.
RefSeqiNP_196963.2. NM_121463.4.
UniGeneiAt.46811.

Genome annotation databases

EnsemblPlantsiAT5G14590.1; AT5G14590.1; AT5G14590.
GeneIDi831311.
KEGGiath:AT5G14590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL163792 Genomic DNA. Translation: CAB87626.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92051.1 .
AY099683 mRNA. Translation: AAM20534.1 .
BT000276 mRNA. Translation: AAN15595.1 .
PIRi T48632.
RefSeqi NP_196963.2. NM_121463.4.
UniGenei At.46811.

3D structure databases

ProteinModelPortali Q8LPJ5.
SMRi Q8LPJ5. Positions 75-475.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G14590.1-P.

Proteomic databases

PaxDbi Q8LPJ5.
PRIDEi Q8LPJ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G14590.1 ; AT5G14590.1 ; AT5G14590 .
GeneIDi 831311.
KEGGi ath:AT5G14590.

Organism-specific databases

GeneFarmi 4411.
TAIRi AT5G14590.

Phylogenomic databases

eggNOGi COG0538.
HOGENOMi HOG000019858.
InParanoidi Q8LPJ5.
KOi K00031.
OMAi CVNTVET.
PhylomeDBi Q8LPJ5.

Enzyme and pathway databases

BioCyci ARA:AT5G14590-MONOMER.
Reactomei REACT_214122. Abnormal conversion of 2-oxoglutarate to 2-hydroxyglutarate.
REACT_218202. NADPH regeneration.

Miscellaneous databases

PROi Q8LPJ5.

Gene expression databases

Genevestigatori Q8LPJ5.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Defining the protein complex proteome of plant mitochondria."
    Klodmann J., Senkler M., Rode C., Braun H.-P.
    Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiICDHP_ARATH
AccessioniPrimary (citable) accession number: Q8LPJ5
Secondary accession number(s): Q9LYK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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