Q8LPI9 (PAI3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic
EC=5.3.1.24

Gene names

Name:	PAI3
Ordered Locus Names:	At1g29410
ORF Names:	F15D2.31

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the TrpF family.
Sequence caution	The sequence AAC49004.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth Inferred from mutant phenotype PubMed 11346937. Source: TAIR multicellular organismal development Inferred from mutant phenotype PubMed 11346937. Source: TAIR response to UV Inferred from expression pattern PubMed 11346937. Source: TAIR tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoribosylanthranilate isomerase activity Inferred from genetic interaction Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8LPI9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8LPI9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     202-213: YLDQLLSFFALS → FNHNNFLLWKRI
     214-244: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Chloroplast Potential
	Chain	33 – 244	212	N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic		PRO_0000417455
Natural variations
	Alternative sequence	202 – 213	12	YLDQL…FFALS → FNHNNFLLWKRI in isoform 2.		VSP_043745
	Alternative sequence	214 – 244	31	Missing in isoform 2.		VSP_043746

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 451E286CCF6E3B40	FASTA	244	26,525
	10 20 30 40 50 60 MSTGISSDLH LHPRALNFSK TSKSGLSNRK VSFSSVGYAQ NRKLSCSVSS TENVAPKDDD 70 80 90 100 110 120 RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SQVAREGGAK 130 140 150 160 170 180 PVGVFVEDDE NTILRAADSS DLELVQLHGN SSRAAFSRLV RERKVIYVLN ANEDGKLLNV 190 200 210 220 230 240 VPEEDGHLAD WILVDSATGG RYLDQLLSFF ALSHCNVFLR GTSYTITLVH ETVCLSQVTE ISRV
	Isoform 2 [UniParc]. Checksum: 10C2087D0F5D358F	FASTA	213	23,221
	10 20 30 40 50 60 MSTGISSDLH LHPRALNFSK TSKSGLSNRK VSFSSVGYAQ NRKLSCSVSS TENVAPKDDD 70 80 90 100 110 120 RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SQVAREGGAK 130 140 150 160 170 180 PVGVFVEDDE NTILRAADSS DLELVQLHGN SSRAAFSRLV RERKVIYVLN ANEDGKLLNV 190 200 210 VPEEDGHLAD WILVDSATGG RFNHNNFLLW KRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes." Li J., Zhao J., Rose A.B., Schmidt R., Last R.L. Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18969 Genomic DNA. Translation: AAC49004.1. Sequence problems. AC068667 Genomic DNA. No translation available. CP002684 Genomic DNA. Translation: AEE31084.1. CP002684 Genomic DNA. Translation: AEE31085.1. AY072443 mRNA. Translation: AAL62435.1. AY099722 mRNA. Translation: AAM20573.1. BT000293 mRNA. Translation: AAN15612.1.
IPI	IPI00536797.
RefSeq	NP_174234.3. NM_102681.3. NP_850954.1. NM_180623.1.
UniGene	At.40744.
3D structure databases
HSSP	HSSP built from PDB template 1LBM based on UniProtKB Q56320.
ProteinModelPortal	Q8LPI9.
SMR	Q8LPI9. Positions 68-179.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G29410.2-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G29410.2; AT1G29410.2; AT1G29410.
GeneID	839817.
KEGG	ath:AT1G29410.
Organism-specific databases
TAIR	At1g29410.
Phylogenomic databases
HOGENOM	HOG000161598.
InParanoid	Q8LPI9.
KO	K01817.
PhylomeDB	Q8LPI9.
ProtClustDB	PLN02363.
Enzyme and pathway databases
UniPathway	UPA00035; UER00042.
Gene expression databases
Genevestigator	Q8LPI9.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001240. PRAI. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00697. PRAI. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAI3_ARATH

Accession

Primary (citable) accession number: Q8LPI9
Secondary accession number(s): Q42528, Q8VY51

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program