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Protein

N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic

Gene

PAI3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.

Pathway:iL-tryptophan biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (At2g04400)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase beta chain 2, chloroplastic (TSB2)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • phosphoribosylanthranilate isomerase activity Source: TAIR

GO - Biological processi

  • growth Source: TAIR
  • multicellular organismal development Source: TAIR
  • response to UV Source: TAIR
  • tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G29410-MONOMER.
UniPathwayiUPA00035; UER00042.

Names & Taxonomyi

Protein namesi
Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (EC:5.3.1.24)
Gene namesi
Name:PAI3
Ordered Locus Names:At1g29410
ORF Names:F15D2.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G29410.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232ChloroplastSequence AnalysisAdd
BLAST
Chaini33 – 244212N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplasticPRO_0000417455Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G29410.2.

Structurei

3D structure databases

ProteinModelPortaliQ8LPI9.
SMRiQ8LPI9. Positions 68-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpF family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000161598.
KOiK01817.
PhylomeDBiQ8LPI9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8LPI9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGISSDLH LHPRALNFSK TSKSGLSNRK VSFSSVGYAQ NRKLSCSVSS
60 70 80 90 100
TENVAPKDDD RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR
110 120 130 140 150
SISLSVAKDI SQVAREGGAK PVGVFVEDDE NTILRAADSS DLELVQLHGN
160 170 180 190 200
SSRAAFSRLV RERKVIYVLN ANEDGKLLNV VPEEDGHLAD WILVDSATGG
210 220 230 240
RYLDQLLSFF ALSHCNVFLR GTSYTITLVH ETVCLSQVTE ISRV
Length:244
Mass (Da):26,525
Last modified:October 1, 2002 - v1
Checksum:i451E286CCF6E3B40
GO
Isoform 2 (identifier: Q8LPI9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     202-213: YLDQLLSFFALS → FNHNNFLLWKRI
     214-244: Missing.

Note: No experimental confirmation available.
Show »
Length:213
Mass (Da):23,221
Checksum:i10C2087D0F5D358F
GO

Sequence cautioni

The sequence AAC49004.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei202 – 21312YLDQL…FFALS → FNHNNFLLWKRI in isoform 2. 1 PublicationVSP_043745Add
BLAST
Alternative sequencei214 – 24431Missing in isoform 2. 1 PublicationVSP_043746Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18969 Genomic DNA. Translation: AAC49004.1. Sequence problems.
AC068667 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE31084.1.
CP002684 Genomic DNA. Translation: AEE31085.1.
AY072443 mRNA. Translation: AAL62435.1.
AY099722 mRNA. Translation: AAM20573.1.
BT000293 mRNA. Translation: AAN15612.1.
RefSeqiNP_174234.3. NM_102681.3. [Q8LPI9-1]
NP_850954.1. NM_180623.1. [Q8LPI9-2]
UniGeneiAt.40744.

Genome annotation databases

EnsemblPlantsiAT1G29410.2; AT1G29410.2; AT1G29410. [Q8LPI9-1]
GeneIDi839817.
KEGGiath:AT1G29410.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18969 Genomic DNA. Translation: AAC49004.1. Sequence problems.
AC068667 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE31084.1.
CP002684 Genomic DNA. Translation: AEE31085.1.
AY072443 mRNA. Translation: AAL62435.1.
AY099722 mRNA. Translation: AAM20573.1.
BT000293 mRNA. Translation: AAN15612.1.
RefSeqiNP_174234.3. NM_102681.3. [Q8LPI9-1]
NP_850954.1. NM_180623.1. [Q8LPI9-2]
UniGeneiAt.40744.

3D structure databases

ProteinModelPortaliQ8LPI9.
SMRiQ8LPI9. Positions 68-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G29410.2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G29410.2; AT1G29410.2; AT1G29410. [Q8LPI9-1]
GeneIDi839817.
KEGGiath:AT1G29410.

Organism-specific databases

TAIRiAT1G29410.

Phylogenomic databases

HOGENOMiHOG000161598.
KOiK01817.
PhylomeDBiQ8LPI9.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00042.
BioCyciARA:AT1G29410-MONOMER.

Miscellaneous databases

PROiQ8LPI9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes."
    Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.
    Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.

Entry informationi

Entry nameiPAI3_ARATH
AccessioniPrimary (citable) accession number: Q8LPI9
Secondary accession number(s): Q42528, Q8VY51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: October 1, 2002
Last modified: June 24, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.