Q8LPF3 (PME68_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectinesterase 68
Short name=PE 68
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase 68
Short name=AtPME68

Gene names

Name:	PME68
Synonyms:	ARATH68
Ordered Locus Names:	At5g47500
ORF Names:	MNJ7.9

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in young siliques. Ref.5
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence BAB09076.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 362	336	Probable pectinesterase 68		PRO_0000371712
Regions
	Compositional bias	46 – 49	4	Poly-His
Sites
	Active site	199	1	Proton donor By similarity
	Active site	220	1	Nucleophile By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity
	Site	198	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8LPF3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C1204DCAD999A3B9	FASTA	362	40,042
	10 20 30 40 50 60 MAQLTNSLNY LFSVSLLLFV SFHCLCFRFS LVAACSNSTD DQQIQHHHHR KWVGPSGHKV 70 80 90 100 110 120 ITVSLNGHAQ FRSVQDAVDS IPKNNNKSIT IKIAPGFYRE KVVVPATKPY ITFKGAGRDV 130 140 150 160 170 180 TAIEWHDRAS DLGANGQQLR TYQTASVTVY ANYFTARNIS FTNTAPAPLP GMQGWQAVAF 190 200 210 220 230 240 RISGDKAFFS GCGFYGAQDT LCDDAGRHYF KECYIEGSID FIFGNGRSMY KDCELHSIAS 250 260 270 280 290 300 RFGSIAAHGR TCPEEKTGFA FVGCRVTGTG PLYVGRAMGQ YSRIVYAYTY FDALVAHGGW 310 320 330 340 350 360 DDWDHKSNKS KTAFFGVYNC YGPGAAATRG VSWARALDYE SAHPFIAKSF VNGRHWIAPR DA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025628 Genomic DNA. Translation: BAB09076.1. Sequence problems. CP002688 Genomic DNA. Translation: AED95526.1. AY102117 mRNA. Translation: AAM26686.1. BT000565 mRNA. Translation: AAN18134.1.
IPI	IPI00521238.
RefSeq	NP_199561.1. NM_124123.4.
UniGene	At.7961.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ProteinModelPortal	Q8LPF3.
SMR	Q8LPF3. Positions 58-360.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8LPF3.
Proteomic databases
PRIDE	Q8LPF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G47500.1; AT5G47500.1; AT5G47500.
GeneID	834800.
GenomeReviews	Gene locus AT5G47500 in contig BA000015_GR.
KEGG	ath:AT5G47500.
NMPDR	fig|3702.1.peg.26559.
Organism-specific databases
GeneFarm	201. 8.
TAIR	At5g47500.
Phylogenomic databases
eggNOG	euNOG09608.
GeneTree	EPGT00070000028060.
HOGENOM	HBG747179.
InParanoid	Q8LPF3.
OMA	FRISGDK.
PhylomeDB	Q8LPF3.
ProtClustDB	PLN02634.
Gene expression databases
Genevestigator	Q8LPF3.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME68_ARATH

Accession

Primary (citable) accession number: Q8LPF3
Secondary accession number(s): Q9FGK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program