Q8LP10 (SPY_EUSGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY
Short name=EgSPY
EC=2.4.1.-

Gene names

Name:

SPY

Organism

Eustoma grandiflorum (Bluebells) (Lisianthus russellianus)

Taxonomic identifier

52518 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Gentianaceae › Chironieae › Eustoma

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins By similarity. May function as a negative regulator of GA signal transduction during vernalization, inhibiting adventitious shoot elongation during vernalization. Ref.1
Catalytic activity	UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Nucleus By similarity.
Induction	During vernalization. Ref.1
Sequence similarities	Belongs to the O-GlcNAc transferase family. Contains 11 TPR repeats.

Ontologies

Keywords
Biological process	Gibberellin signaling pathway
Cellular component	Nucleus
Domain	Repeat TPR repeat
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY		PRO_0000191777
Regions
	Repeat	34 – 66	33	TPR 1
	Repeat	67 – 99	33	TPR 2
	Repeat	101 – 132	32	TPR 3
	Repeat	140 – 171	32	TPR 4
	Repeat	172 – 205	34	TPR 5
	Repeat	207 – 238	32	TPR 6
	Repeat	239 – 271	33	TPR 7
	Repeat	273 – 305	33	TPR 8
	Repeat	306 – 339	34	TPR 9
	Repeat	341 – 373	33	TPR 10
	Repeat	374 – 407	34	TPR 11
	Region	408 – 918	511	Catalytic region

Sequences

	Sequence		Length	Mass (Da)
	Q8LP10 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D79209A7A6844F89	FASTA	918	102,154
	10 20 30 40 50 60 MIEKAIVAKP KGLLKGVQSS SDSKGSPVKK SLEGKEAITY AKILRSRNKF VDALAIYELE 70 80 90 100 110 120 KDSKNVEAHI GKGICLQTQN KGNLAFDCFS EAIRLDPHNA CALTHCGILY KDEGRLVEAA 130 140 150 160 170 180 SYQKALQADP SYKPAAECLA TVLNDLGTSL KGNTQEGIQK YYEAVKIDPH YAPACYNLGV 190 200 210 220 230 240 VYSEMMQYDV ALSCYERAAT ESPTYADAYC NTGIIYKNRG DLCLAVSPNF EIAKNNMGIA 250 260 270 280 290 300 LTDLGTKEKL EGDIDQGVAY YKKALYYNWH YSDAMYNLGV AYGEMLKFDM AIIFDELAFH 310 320 330 340 350 360 FNPHCAEACN NLGVIYKDRD NLDKAVECYQ KALSIKPNFS QSLNNLGVVF TVQGKMDAAA 370 380 390 400 410 420 SMIEKAIVAN PTYAEAYNNL GVLYRDAGNI FLAIEAYEQC LKIDPDSRNA GQNRLLAMNY 430 440 450 460 470 480 INEGADDRLY EAHRDWGGRF MRLYSQYTSW DNPKDPERPL VIGYGSPDHF LSYFIEAPLL 490 500 510 520 530 540 YHDYENFKVV TYSAVVKADA KTNRFRERVL KKGGIWRDIY GIDEKKVASM IREDKVDILI 550 560 570 580 590 600 ELTGHTANNK LGMMACRPAP IQVTWIGYPN TTGLPTIDYR ITDSLADPLD TKQKHVEELI 610 620 630 640 650 660 QLPACFLCYT PSPEAGPVSP TPALSNGFIT FGSFNNLAKI TPKVLQVWAR ILCAVSNSRL 670 680 690 700 710 720 IVKCKPFCCE SVRQTFLSTL EQLGLESTRV DLLPLILLNH DHMQAYSLMD ISLDTFPYAG 730 740 750 760 770 780 TTTTCESLYM GVPCITMRGL VHAHNVGVSL LSTVGLGHLV AKNEDDYVRL AVQLASDVTA 790 800 810 820 830 840 LSNLRLTLRE LMSKSPLCDG PKFIQDLELT YRSMWHRYCK GDIPSLSRME ILQKEELDVV 850 860 870 880 890 900 QEQLHQQPNT SPQKLVKDEP ADDASGPEHG PASKDNPLVL IKINGYNTSP SSITSPSSEE 910 NGVSQTRMLN CGDQCFRV

References

[1]

"Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum." Mino M., Oka M., Tasaka Y., Iwabuchi M. Plant Cell Rep. 22:159-165(2003) [PubMed: 12879260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB080739 mRNA. Translation: BAC11808.1.
3D structure databases
ProteinModelPortal	Q8LP10.
ModBase	Search...
Protein family/group databases
CAZy	GT41. Glycosyltransferase Family 41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006597. Sel1-like. IPR001440. TPR-1. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 2 hits.
Pfam	PF08238. Sel1. 1 hit. PF00515. TPR_1. 4 hits. [Graphical view]
SMART	SM00671. SEL1. 3 hits. SM00028. TPR. 9 hits. [Graphical view]
PROSITE	PS50005. TPR. 9 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPY_EUSGR

Accession

Primary (citable) accession number: Q8LP10

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2002
Last modified:	September 21, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program