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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein B

Gene

XTHB

Organism
Phaseolus angularis (Azuki bean) (Vigna angularis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106NucleophilePROSITE-ProRule annotation1
Active sitei110Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein B (EC:2.4.1.207)
Alternative name(s):
VaXTH2
Gene namesi
Name:XTHB
Synonyms:XTH2
OrganismiPhaseolus angularis (Azuki bean) (Vigna angularis)
Taxonomic identifieri3914 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001183822 – 293Probable xyloglucan endotransglucosylase/hydrolase protein BAdd BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Predominantly expressed in the phloem fibers of growing internodes. Weakly or not expressed in the xylem. In the internode, it is expressed closer to the bottom of the internode compared to XTHA.1 Publication

Inductioni

Up-regulated by indole-3-acetic acid (IAA). Effect of IAA is not affected by mannitol. Not up-regulated by fusicoccin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8LNZ5
SMRiQ8LNZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 220GH16PROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LNZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLLILCL VLVSLASSAL CAAPRRPVDV PFGRNYIPTW AFDHIKYFNG
60 70 80 90 100
GSEIQLHLDK YTGTGFQTKG SYLFGHFSMN IKMVPGDSAG TVTAFCLSSQ
110 120 130 140 150
NAEHDEIDFE FLGNRTGQPY ILQTNVFTGG KGDREQRIYL WFDPTKAYHR
160 170 180 190 200
YSVLWNMYQI VFLVDNIPIR VFKNLKELGV KFPFNQPMKV YNSLWNADDW
210 220 230 240 250
ATRGGLEKTD WSKAPFVAEY KGFHVDGCEA SVNSRFCATQ GKRWWDQTEF
260 270 280 290
RDLDSFQWRR LKWVRQKFTI YNYCTDRTRY PQLPPECRRN RDI
Length:293
Mass (Da):34,096
Last modified:October 1, 2002 - v1
Checksum:iBD2FA9A049211CD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086396 Genomic DNA Translation: BAC03238.1

Similar proteinsi

Entry informationi

Entry nameiXTHB_PHAAN
AccessioniPrimary (citable) accession number: Q8LNZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health