Q8LNZ5 (XTHB_PHAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein B
EC=2.4.1.207

Alternative name(s):
VaXTH2

Gene names

Name:	XTHB
Synonyms:	XTH2

Organism

Phaseolus angularis (Azuki bean) (Vigna angularis)

Taxonomic identifier

3914 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Predominantly expressed in the phloem fibers of growing internodes. Weakly or not expressed in the xylem. In the internode, it is expressed closer to the bottom of the internode compared to XTHA. Ref.1
Induction	Up-regulated by indole-3-acetic acid (IAA). Effect of IAA is not affected by mannitol. Not up-regulated by fusicoccin. Ref.1
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 293	272	Probable xyloglucan endotransglucosylase/hydrolase protein B		PRO_0000011838
Sites
	Active site	106	1	Nucleophile By similarity
	Active site	110	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8LNZ5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: BD2FA9A049211CD5	FASTA	293	34,096
	10 20 30 40 50 60 MASSLLILCL VLVSLASSAL CAAPRRPVDV PFGRNYIPTW AFDHIKYFNG GSEIQLHLDK 70 80 90 100 110 120 YTGTGFQTKG SYLFGHFSMN IKMVPGDSAG TVTAFCLSSQ NAEHDEIDFE FLGNRTGQPY 130 140 150 160 170 180 ILQTNVFTGG KGDREQRIYL WFDPTKAYHR YSVLWNMYQI VFLVDNIPIR VFKNLKELGV 190 200 210 220 230 240 KFPFNQPMKV YNSLWNADDW ATRGGLEKTD WSKAPFVAEY KGFHVDGCEA SVNSRFCATQ 250 260 270 280 290 GKRWWDQTEF RDLDSFQWRR LKWVRQKFTI YNYCTDRTRY PQLPPECRRN RDI

References

[1]

"Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin." Nakamura T., Yokoyama R., Tomita E., Nishitani K. Plant Cell Physiol. 44:16-24(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Takara Wase.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB086396 Genomic DNA. Translation: BAC03238.1.
3D structure databases
ProteinModelPortal	Q8LNZ5.
SMR	Q8LNZ5. Positions 27-293.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTHB_PHAAN

Accession

Primary (citable) accession number: Q8LNZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2002
Last modified:	March 6, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program