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Q8LNZ5 (XTHB_PHAAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein B

EC=2.4.1.207
Alternative name(s):
VaXTH2
Gene names
Name:XTHB
Synonyms:XTH2
OrganismPhaseolus angularis (Azuki bean) (Vigna angularis)
Taxonomic identifier3914 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Predominantly expressed in the phloem fibers of growing internodes. Weakly or not expressed in the xylem. In the internode, it is expressed closer to the bottom of the internode compared to XTHA. Ref.1

Induction

Up-regulated by indole-3-acetic acid (IAA). Effect of IAA is not affected by mannitol. Not up-regulated by fusicoccin. Ref.1

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 293272Probable xyloglucan endotransglucosylase/hydrolase protein B
PRO_0000011838

Sites

Active site1061Nucleophile By similarity
Active site1101Proton donor By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8LNZ5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: BD2FA9A049211CD5

FASTA29334,096
        10         20         30         40         50         60 
MASSLLILCL VLVSLASSAL CAAPRRPVDV PFGRNYIPTW AFDHIKYFNG GSEIQLHLDK 

        70         80         90        100        110        120 
YTGTGFQTKG SYLFGHFSMN IKMVPGDSAG TVTAFCLSSQ NAEHDEIDFE FLGNRTGQPY 

       130        140        150        160        170        180 
ILQTNVFTGG KGDREQRIYL WFDPTKAYHR YSVLWNMYQI VFLVDNIPIR VFKNLKELGV 

       190        200        210        220        230        240 
KFPFNQPMKV YNSLWNADDW ATRGGLEKTD WSKAPFVAEY KGFHVDGCEA SVNSRFCATQ 

       250        260        270        280        290 
GKRWWDQTEF RDLDSFQWRR LKWVRQKFTI YNYCTDRTRY PQLPPECRRN RDI 

« Hide

References

[1]"Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin."
Nakamura T., Yokoyama R., Tomita E., Nishitani K.
Plant Cell Physiol. 44:16-24(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Takara Wase.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB086396 Genomic DNA. Translation: BAC03238.1.

3D structure databases

ProteinModelPortalQ8LNZ5.
SMRQ8LNZ5. Positions 27-293.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTHB_PHAAN
AccessionPrimary (citable) accession number: Q8LNZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries