Reviewed, UniProtKB/Swiss-Prot Q8LL87 (COMT1_COFCA)

Names and origin

Protein names	Recommended name:     Caffeic acid 3-O-methyltransferase     EC=2.1.1.68 Alternative name(s):     S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase       Short name=CAOMT       Short name=COMT
Organism	Coffea canephora (Robusta coffee)
Taxonomic identifier	49390 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Rubiaceae › Ixoroideae › Coffeeae › Coffea

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Caffeic acid 3-O-methyltransferase		PRO_0000063200
Regions
	Region	117 – 123	7	Substrate binding By similarity
	Region	149 – 167	19	Substrate binding By similarity
Sites
	Active site	256	1	Proton acceptor By similarity
	Binding site	195	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	218	1	S-adenosyl-L-methionine By similarity
	Binding site	238	1	S-adenosyl-L-methionine By similarity
	Binding site	239	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	252	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	230	1	S → P in AAN03726. Ref.1
	Sequence conflict	268	1	K → R in AAN03726. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8LL87-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D6AC10007BDF8E6B	FASTA	350	38,258
	10 20 30 40 50 60 MAEEEACLFA MSLASASVLP MVLKSAIELD LLELIAKAGP GAYVSPSELA AQLPTHNPEA 70 80 90 100 110 120 PIMLDRILRL LATYSVLDCK LNNLADGGVE RLYGLAPVCK FLTKNADGVS MAPLLLMNQD 130 140 150 160 170 180 KVLMESWYHL KDAVLDGGIP FNKAYGMTAF EYHGTDPRFN KVFNQGMSNH STITMKKILE 190 200 210 220 230 240 VYRGFEGLKT VVDVGGGTGA TLNMIISKYP TIKGINFELP HVVEDAPSHS GVEHVGGDMF 250 260 270 280 290 300 VSVPKGDAIF MKWICHDWSD DHCRKLLKNC YQALPDNGKV ILAECVLPEA PDTSLATQNV 310 320 330 340 350 VHVDVVMLAH NPGGKERTEK EFEALAKGAG FKEFRKVCSA VNTWIMELCK

References

[1]	"Complete cDNA sequence of a caffeic acid O-methyltransferase from Coffea canephora fruit." Campa C., Legal L., Khounlotham M., Noirot M., de Kochko A. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fruit and Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF454631 mRNA. Translation: AAN03726.1. AF454632 mRNA. Translation: AAN03727.1.
3D structure databases
HSSP	HSSP built from PDB template 1KYZ based on UniProtKB P28002.
SMR	Q8LL87. Positions 1-350.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.68. 275282.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT1_COFCA

Accession

Primary (citable) accession number: Q8LL87
Secondary accession number(s): Q8LL88

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	October 1, 2002
Last modified:	January 20, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)