Reviewed, UniProtKB/Swiss-Prot Q8LL69 (DBNBT_TAXCA)

Names and origin

Protein names

Recommended name:
    3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase
      Short name=DBTNBT
    EC=2.3.1.-

Gene names

Name:

TAX10

Organism

Taxus canadensis (Canadian yew)

Taxonomic identifier

88032 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Taxaceae › Taxus

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the stereoselective coupling of the surrogate substrate N-debenzoyl-(3'RS)-2'-deoxytaxol with benzoyl-CoA to form predominantly one 3'-epimer of 2'-deoxytaxol.
Pathway	Alkaloid biosynthesis; taxol biosynthesis.
Sequence similarities	Belongs to the plant acetyltransferase family.

Ontologies

Keywords
Biological process	Taxol biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	paclitaxel biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase		PRO_0000147361

Sequences

	Sequence		Length	Mass (Da)
	Q8LL69-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1F0C200FDA6D4A0C	FASTA	441	49,041
	10 20 30 40 50 60 MEKAGSTDFH VKKFDPVMVA PSLPSPKATV QLSVVDSLTI CRGIFNTLLV FNAPDNISAD 70 80 90 100 110 120 PVKIIREALS KVLVYYFPLA GRLRSKEIGE LEVECTGDGA LFVEAMVEDT ISVLRDLDDL 130 140 150 160 170 180 NPSFQQLVFW HPLDTAIEDL HLVIVQVTRF TCGGIAVGVT LPHSVCDGRG AAQFVTALAE 190 200 210 220 230 240 MARGEVKPSL EPIWNRELLN PEDPLHLQLN QFDSICPPPM LEELGQASFV INVDTIEYMK 250 260 270 280 290 300 QCVMEECNEF CSSFEVVAAL VWIARTKALQ IPHTENVKLL FAMDLRKLFN PPLPNGYYGN 310 320 330 340 350 360 AIGTAYAMDN VQDLLNGSLL RAIMIIKKAK ADLKDNYSRS RVVTNPYSLD VNKKSDNILA 370 380 390 400 410 420 LSDWRRLGFY EADFGWGGPL NVSSLQRLEN GLPMFSTFLY LLPAKNKSDG IKLLLSCMPP 430 440 TTLKSFKIVM EAMIEKYVSK V

References

[1]	"The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus." Walker K., Long R., Croteau R.B. Proc. Natl. Acad. Sci. U.S.A. 99:9166-9171(2002) [PubMed: 12089320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF466397 mRNA. Translation: AAM75818.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13418.
Family and domain databases
InterPro	IPR003480. Transferase. [Graphical view]
Pfam	PF02458. Transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DBNBT_TAXCA

Accession

Primary (citable) accession number: Q8LL69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	October 1, 2002
Last modified:	November 24, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)