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Protein

RNA polymerase II C-terminal domain phosphatase-like 3

Gene

CPL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II (By similarity). Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated signaling pathway and cold resistance.By similarity2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mg2+By similarity, Co2+By similarity, Mn2+By similarityNote: Binds Mg(2+), Co(2+) or Mn2+.By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-EC
  3. protein C-terminus binding Source: UniProtKB
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. response to salt stress Source: UniProtKB
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT2G33540-MONOMER.
ReactomeiREACT_259973. RNA Polymerase II Pre-transcription Events.
REACT_260665. Formation of the Early Elongation Complex.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 3 (EC:3.1.3.16)
Short name:
FCP-like 3
Alternative name(s):
Carboxyl-terminal phosphatase-like 3
Short name:
AtCPL3
Short name:
CTD phosphatase-like 3
Gene namesi
Name:CPL3
Ordered Locus Names:At2g33540
ORF Names:F4P9.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G33540.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Grows more slowly and flower earlier than wild-type plants. ABA hyperactivation of stress-inducible genes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12411241RNA polymerase II C-terminal domain phosphatase-like 3PRO_0000376085Add
BLAST

Proteomic databases

PaxDbiQ8LL04.
PRIDEiQ8LL04.

Expressioni

Inductioni

By NaCl.1 Publication

Gene expression databases

GenevestigatoriQ8LL04.

Interactioni

Subunit structurei

Interacts with RAP74.1 Publication

Protein-protein interaction databases

BioGridi3266. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8LL04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini923 – 1103181FCP1 homologyPROSITE-ProRule annotationAdd
BLAST
Domaini1146 – 123994BRCTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi64 – 729Poly-Gly

Domaini

The BRCT domain is required for interaction with RAP74.

Sequence similaritiesi

Contains 1 BRCT domain.PROSITE-ProRule annotation
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000085035.
InParanoidiQ8LL04.
OMAiMRVTVTP.
PhylomeDBiQ8LL04.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LL04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVARSGCSR TLIRMGNDEN LMVMVDVEEG EIPDSVNTEI EVKHKSTTTT
60 70 80 90 100
ADVGGDVDVG VVAGGRGGGG GGSNGNSRVW TMEELISQYP AYRPYANSGL
110 120 130 140 150
SNLAWARAVQ NKPFNEGLVM DYEPRESDKI VIEDSDDEKE EGELEEGEID
160 170 180 190 200
LVDNASDDNL VEKDTESVVL ISADKVEDDR ILKERDLEKK VKLIRGVLES
210 220 230 240 250
TSLVEAQTGF EGVCSRILGA LESLRELVSD NDDFPKRDTL VQLSFASLQT
260 270 280 290 300
INYVFCSMNN ISKERNKETM SRLLTLVNDH FSQFLSFNQK NEIETMNQDL
310 320 330 340 350
SRSAIAVFAG TSSEENVNQM TQPSNGDSFL AKKLTSESTH RGAAYLRSRL
360 370 380 390 400
PMLPLLDLHK DHDADSLPSP TRETTPSLPV NGRHTMVRPG FPVGRESQTT
410 420 430 440 450
EGAKVYSYES DARKAVSTYQ QKFGLNSVFK TDDLPSPTPS GEPNDGNGDV
460 470 480 490 500
GGEVSSSVVK SSNPGSHLIY GQDVPLPSNF NSRSMPVANS VSSTVPPHHL
510 520 530 540 550
SIHAISAPTA SDQTVKPSAK SRDPRLRLAK PDAANVTIYS YSSGDARNLS
560 570 580 590 600
KVELSADLVN PRKQKAADEF LIDGPAWKRQ KSDTDAPKAA GTGGWLEDTE
610 620 630 640 650
SSGLLKLESK PRLIENGVTS MTSSVMPTSA VSVSQKVRTA STDTASLQSL
660 670 680 690 700
LKDIAVNPTM LLNLLKMGER QKVPEKAIQK PMDPRRAAQL PGSSVQPGVS
710 720 730 740 750
TPLSIPASNA LAANSLNSGV LQDSSQNAPA AESGSIRMKP RDPRRILHGS
760 770 780 790 800
TLQRTDSSME KQTKVNDPST LGTLTMKGKA EDLETPPQLD PRQNISQNGT
810 820 830 840 850
SKMKISGELL SGKTPDFSTQ FTKNLKSIAD MVVVSQQLGN PPASMHSVQL
860 870 880 890 900
KTERDVKHNP SNPNAQDEDV SVSAASVTAA AGPTRSMNSW GDVEHLFEGY
910 920 930 940 950
DDIQRVAIQR ERVRRLEEQN KMFASQKLSL VLDIDHTLLN SAKFNEVESR
960 970 980 990 1000
HEEILRKKEE QDREKPYRHL FRFLHMGMWT KLRPGIWNFL EKASKLYELH
1010 1020 1030 1040 1050
LYTMGNKLYA TEMAKLLDPK GVLFNGRVIS KGDDGDPLDG DERVPKSKDL
1060 1070 1080 1090 1100
EGVMGMESSV VIIDDSVRVW PQHKMNLIAV ERYLYFPCSR RQFGLLGPSL
1110 1120 1130 1140 1150
LELDRDEVPE EGTLASSLAV IEKIHQNFFS HTSLDEVDVR NILASEQRKI
1160 1170 1180 1190 1200
LAGCRIVFSR IIPVGEAKPH LHPLWQTAEQ FGAVCTTQVD EHVTHVVTNS
1210 1220 1230 1240
LGTDKVNWAL TRGRFVVHPG WVEASAFLYQ RANENLYAIN P
Length:1,241
Mass (Da):136,479
Last modified:May 26, 2009 - v2
Checksum:iCEFFBD725719D404
GO

Sequence cautioni

The sequence AAB80671.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1010 – 10101A → V in AAM94371. (PubMed:12149434)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486633 mRNA. Translation: AAM94371.1.
AC002332 Genomic DNA. Translation: AAB80671.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08850.1.
PIRiG84746.
RefSeqiNP_180912.2. NM_128914.2.
UniGeneiAt.43643.

Genome annotation databases

EnsemblPlantsiAT2G33540.1; AT2G33540.1; AT2G33540.
GeneIDi817919.
KEGGiath:AT2G33540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486633 mRNA. Translation: AAM94371.1.
AC002332 Genomic DNA. Translation: AAB80671.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08850.1.
PIRiG84746.
RefSeqiNP_180912.2. NM_128914.2.
UniGeneiAt.43643.

3D structure databases

ProteinModelPortaliQ8LL04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3266. 1 interaction.

Proteomic databases

PaxDbiQ8LL04.
PRIDEiQ8LL04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G33540.1; AT2G33540.1; AT2G33540.
GeneIDi817919.
KEGGiath:AT2G33540.

Organism-specific databases

TAIRiAT2G33540.

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000085035.
InParanoidiQ8LL04.
OMAiMRVTVTP.
PhylomeDBiQ8LL04.

Enzyme and pathway databases

BioCyciARA:AT2G33540-MONOMER.
ReactomeiREACT_259973. RNA Polymerase II Pre-transcription Events.
REACT_260665. Formation of the Early Elongation Complex.

Gene expression databases

GenevestigatoriQ8LL04.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development."
    Koiwa H., Barb A.W., Xiong L., Li F., McCully M.G., Lee B.-H., Sokolchik I., Zhu J., Gong Z., Reddy M., Sharkhuu A., Manabe Y., Yokoi S., Zhu J.-K., Bressan R.A., Hasegawa P.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:10893-10898(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions."
    Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M., Bahk J., Koiwa H.
    Plant Physiol. 142:586-594(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RAP74, DISRUPTION PHENOTYPE, INDUCTION BY NACL, BRCT DOMAIN, SUBCELLULAR LOCATION.
  5. "Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
    Kerk D., Templeton G., Moorhead G.B.G.
    Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCPL3_ARATH
AccessioniPrimary (citable) accession number: Q8LL04
Secondary accession number(s): O22804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: January 7, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.