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Q8LL04

- CPL3_ARATH

UniProt

Q8LL04 - CPL3_ARATH

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Protein
RNA polymerase II C-terminal domain phosphatase-like 3
Gene
CPL3, At2g33540, F4P9.31
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II By similarity. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated signaling pathway and cold resistance.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Binds magnesium, cobalt or manganese ions By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoprotein phosphatase activity Source: UniProtKB-EC
  4. protein C-terminus binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. response to salt stress Source: UniProtKB
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT2G33540-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 3 (EC:3.1.3.16)
Short name:
FCP-like 3
Alternative name(s):
Carboxyl-terminal phosphatase-like 3
Short name:
AtCPL3
Short name:
CTD phosphatase-like 3
Gene namesi
Name:CPL3
Ordered Locus Names:At2g33540
ORF Names:F4P9.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G33540.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Grows more slowly and flower earlier than wild-type plants. ABA hyperactivation of stress-inducible genes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12411241RNA polymerase II C-terminal domain phosphatase-like 3
PRO_0000376085Add
BLAST

Proteomic databases

PaxDbiQ8LL04.
PRIDEiQ8LL04.

Expressioni

Inductioni

By NaCl.1 Publication

Gene expression databases

GenevestigatoriQ8LL04.

Interactioni

Subunit structurei

Interacts with RAP74.1 Publication

Protein-protein interaction databases

BioGridi3266. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8LL04.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini923 – 1103181FCP1 homology
Add
BLAST
Domaini1146 – 123994BRCT
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi64 – 729Poly-Gly

Domaini

The BRCT domain is required for interaction with RAP74.1 Publication

Sequence similaritiesi

Contains 1 BRCT domain.

Phylogenomic databases

eggNOGiCOG5190.
HOGENOMiHOG000085035.
InParanoidiQ8LL04.
OMAiCTNQIDD.
PhylomeDBiQ8LL04.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LL04-1 [UniParc]FASTAAdd to Basket

« Hide

MLVARSGCSR TLIRMGNDEN LMVMVDVEEG EIPDSVNTEI EVKHKSTTTT     50
ADVGGDVDVG VVAGGRGGGG GGSNGNSRVW TMEELISQYP AYRPYANSGL 100
SNLAWARAVQ NKPFNEGLVM DYEPRESDKI VIEDSDDEKE EGELEEGEID 150
LVDNASDDNL VEKDTESVVL ISADKVEDDR ILKERDLEKK VKLIRGVLES 200
TSLVEAQTGF EGVCSRILGA LESLRELVSD NDDFPKRDTL VQLSFASLQT 250
INYVFCSMNN ISKERNKETM SRLLTLVNDH FSQFLSFNQK NEIETMNQDL 300
SRSAIAVFAG TSSEENVNQM TQPSNGDSFL AKKLTSESTH RGAAYLRSRL 350
PMLPLLDLHK DHDADSLPSP TRETTPSLPV NGRHTMVRPG FPVGRESQTT 400
EGAKVYSYES DARKAVSTYQ QKFGLNSVFK TDDLPSPTPS GEPNDGNGDV 450
GGEVSSSVVK SSNPGSHLIY GQDVPLPSNF NSRSMPVANS VSSTVPPHHL 500
SIHAISAPTA SDQTVKPSAK SRDPRLRLAK PDAANVTIYS YSSGDARNLS 550
KVELSADLVN PRKQKAADEF LIDGPAWKRQ KSDTDAPKAA GTGGWLEDTE 600
SSGLLKLESK PRLIENGVTS MTSSVMPTSA VSVSQKVRTA STDTASLQSL 650
LKDIAVNPTM LLNLLKMGER QKVPEKAIQK PMDPRRAAQL PGSSVQPGVS 700
TPLSIPASNA LAANSLNSGV LQDSSQNAPA AESGSIRMKP RDPRRILHGS 750
TLQRTDSSME KQTKVNDPST LGTLTMKGKA EDLETPPQLD PRQNISQNGT 800
SKMKISGELL SGKTPDFSTQ FTKNLKSIAD MVVVSQQLGN PPASMHSVQL 850
KTERDVKHNP SNPNAQDEDV SVSAASVTAA AGPTRSMNSW GDVEHLFEGY 900
DDIQRVAIQR ERVRRLEEQN KMFASQKLSL VLDIDHTLLN SAKFNEVESR 950
HEEILRKKEE QDREKPYRHL FRFLHMGMWT KLRPGIWNFL EKASKLYELH 1000
LYTMGNKLYA TEMAKLLDPK GVLFNGRVIS KGDDGDPLDG DERVPKSKDL 1050
EGVMGMESSV VIIDDSVRVW PQHKMNLIAV ERYLYFPCSR RQFGLLGPSL 1100
LELDRDEVPE EGTLASSLAV IEKIHQNFFS HTSLDEVDVR NILASEQRKI 1150
LAGCRIVFSR IIPVGEAKPH LHPLWQTAEQ FGAVCTTQVD EHVTHVVTNS 1200
LGTDKVNWAL TRGRFVVHPG WVEASAFLYQ RANENLYAIN P 1241
Length:1,241
Mass (Da):136,479
Last modified:May 26, 2009 - v2
Checksum:iCEFFBD725719D404
GO

Sequence cautioni

The sequence AAB80671.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1010 – 10101A → V in AAM94371. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF486633 mRNA. Translation: AAM94371.1.
AC002332 Genomic DNA. Translation: AAB80671.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08850.1.
PIRiG84746.
RefSeqiNP_180912.2. NM_128914.2.
UniGeneiAt.43643.

Genome annotation databases

EnsemblPlantsiAT2G33540.1; AT2G33540.1; AT2G33540.
GeneIDi817919.
KEGGiath:AT2G33540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF486633 mRNA. Translation: AAM94371.1 .
AC002332 Genomic DNA. Translation: AAB80671.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC08850.1 .
PIRi G84746.
RefSeqi NP_180912.2. NM_128914.2.
UniGenei At.43643.

3D structure databases

ProteinModelPortali Q8LL04.
ModBasei Search...

Protein-protein interaction databases

BioGridi 3266. 1 interaction.

Proteomic databases

PaxDbi Q8LL04.
PRIDEi Q8LL04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G33540.1 ; AT2G33540.1 ; AT2G33540 .
GeneIDi 817919.
KEGGi ath:AT2G33540.

Organism-specific databases

TAIRi AT2G33540.

Phylogenomic databases

eggNOGi COG5190.
HOGENOMi HOG000085035.
InParanoidi Q8LL04.
OMAi CTNQIDD.
PhylomeDBi Q8LL04.

Enzyme and pathway databases

BioCyci ARA:AT2G33540-MONOMER.

Gene expression databases

Genevestigatori Q8LL04.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view ]
SUPFAMi SSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR02250. FCP1_euk. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development."
    Koiwa H., Barb A.W., Xiong L., Li F., McCully M.G., Lee B.-H., Sokolchik I., Zhu J., Gong Z., Reddy M., Sharkhuu A., Manabe Y., Yokoi S., Zhu J.-K., Bressan R.A., Hasegawa P.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:10893-10898(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis carboxyl-terminal domain phosphatase-like isoforms share common catalytic and interaction domains but have distinct in planta functions."
    Bang W., Kim S., Ueda A., Vikram M., Yun D., Bressan R.A., Hasegawa P.M., Bahk J., Koiwa H.
    Plant Physiol. 142:586-594(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RAP74, DISRUPTION PHENOTYPE, INDUCTION BY NACL, BRCT DOMAIN, SUBCELLULAR LOCATION.
  5. "Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
    Kerk D., Templeton G., Moorhead G.B.G.
    Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCPL3_ARATH
AccessioniPrimary (citable) accession number: Q8LL04
Secondary accession number(s): O22804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: May 14, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi