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Protein

RNA polymerase II C-terminal domain phosphatase-like 3

Gene

CPL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and/or 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II (By similarity). Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated signaling pathway and cold resistance.By similarity2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mg2+By similarity, Co2+By similarity, Mn2+By similarityNote: Binds Mg2+, Co2+ or Mn2+.By similarity

GO - Molecular functioni

  • CTD phosphatase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • protein C-terminus binding Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • dephosphorylation of RNA polymerase II C-terminal domain Source: GO_Central
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • response to salt stress Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase, Repressor, RNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-ATH-113418 Formation of the Early Elongation Complex
R-ATH-674695 RNA Polymerase II Pre-transcription Events
R-ATH-6796648 TP53 Regulates Transcription of DNA Repair Genes

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 3 (EC:3.1.3.16)
Short name:
FCP-like 3
Alternative name(s):
Carboxyl-terminal phosphatase-like 3
Short name:
AtCPL3
Short name:
CTD phosphatase-like 3
Gene namesi
Name:CPL3
Ordered Locus Names:At2g33540
ORF Names:F4P9.31
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G33540
TAIRilocus:2051164 AT2G33540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Grows more slowly and flower earlier than wild-type plants. ABA hyperactivation of stress-inducible genes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003760851 – 1241RNA polymerase II C-terminal domain phosphatase-like 3Add BLAST1241

Proteomic databases

PaxDbiQ8LL04
PRIDEiQ8LL04

PTM databases

iPTMnetiQ8LL04

Expressioni

Inductioni

By NaCl.1 Publication

Gene expression databases

ExpressionAtlasiQ8LL04 baseline and differential
GenevisibleiQ8LL04 AT

Interactioni

Subunit structurei

Interacts with RAP74.1 Publication

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi3266, 2 interactors
STRINGi3702.AT2G33540.1

Structurei

3D structure databases

ProteinModelPortaliQ8LL04
SMRiQ8LL04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini923 – 1103FCP1 homologyPROSITE-ProRule annotationAdd BLAST181
Domaini1146 – 1239BRCTPROSITE-ProRule annotationAdd BLAST94

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi64 – 72Poly-Gly9

Domaini

The BRCT domain is required for interaction with RAP74.

Phylogenomic databases

eggNOGiKOG0323 Eukaryota
COG5190 LUCA
HOGENOMiHOG000085035
InParanoidiQ8LL04
KOiK18999
OMAiVIRMKPR
OrthoDBiEOG093600P5
PhylomeDBiQ8LL04

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.1000, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR004274 FCP1_dom
IPR011947 FCP1_euk
IPR036412 HAD-like_sf
IPR023214 HAD_sf
PfamiView protein in Pfam
PF03031 NIF, 1 hit
PF12738 PTCB-BRCT, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00577 CPDc, 1 hit
SUPFAMiSSF52113 SSF52113, 1 hit
SSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR02250 FCP1_euk, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS50969 FCP1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8LL04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVARSGCSR TLIRMGNDEN LMVMVDVEEG EIPDSVNTEI EVKHKSTTTT
60 70 80 90 100
ADVGGDVDVG VVAGGRGGGG GGSNGNSRVW TMEELISQYP AYRPYANSGL
110 120 130 140 150
SNLAWARAVQ NKPFNEGLVM DYEPRESDKI VIEDSDDEKE EGELEEGEID
160 170 180 190 200
LVDNASDDNL VEKDTESVVL ISADKVEDDR ILKERDLEKK VKLIRGVLES
210 220 230 240 250
TSLVEAQTGF EGVCSRILGA LESLRELVSD NDDFPKRDTL VQLSFASLQT
260 270 280 290 300
INYVFCSMNN ISKERNKETM SRLLTLVNDH FSQFLSFNQK NEIETMNQDL
310 320 330 340 350
SRSAIAVFAG TSSEENVNQM TQPSNGDSFL AKKLTSESTH RGAAYLRSRL
360 370 380 390 400
PMLPLLDLHK DHDADSLPSP TRETTPSLPV NGRHTMVRPG FPVGRESQTT
410 420 430 440 450
EGAKVYSYES DARKAVSTYQ QKFGLNSVFK TDDLPSPTPS GEPNDGNGDV
460 470 480 490 500
GGEVSSSVVK SSNPGSHLIY GQDVPLPSNF NSRSMPVANS VSSTVPPHHL
510 520 530 540 550
SIHAISAPTA SDQTVKPSAK SRDPRLRLAK PDAANVTIYS YSSGDARNLS
560 570 580 590 600
KVELSADLVN PRKQKAADEF LIDGPAWKRQ KSDTDAPKAA GTGGWLEDTE
610 620 630 640 650
SSGLLKLESK PRLIENGVTS MTSSVMPTSA VSVSQKVRTA STDTASLQSL
660 670 680 690 700
LKDIAVNPTM LLNLLKMGER QKVPEKAIQK PMDPRRAAQL PGSSVQPGVS
710 720 730 740 750
TPLSIPASNA LAANSLNSGV LQDSSQNAPA AESGSIRMKP RDPRRILHGS
760 770 780 790 800
TLQRTDSSME KQTKVNDPST LGTLTMKGKA EDLETPPQLD PRQNISQNGT
810 820 830 840 850
SKMKISGELL SGKTPDFSTQ FTKNLKSIAD MVVVSQQLGN PPASMHSVQL
860 870 880 890 900
KTERDVKHNP SNPNAQDEDV SVSAASVTAA AGPTRSMNSW GDVEHLFEGY
910 920 930 940 950
DDIQRVAIQR ERVRRLEEQN KMFASQKLSL VLDIDHTLLN SAKFNEVESR
960 970 980 990 1000
HEEILRKKEE QDREKPYRHL FRFLHMGMWT KLRPGIWNFL EKASKLYELH
1010 1020 1030 1040 1050
LYTMGNKLYA TEMAKLLDPK GVLFNGRVIS KGDDGDPLDG DERVPKSKDL
1060 1070 1080 1090 1100
EGVMGMESSV VIIDDSVRVW PQHKMNLIAV ERYLYFPCSR RQFGLLGPSL
1110 1120 1130 1140 1150
LELDRDEVPE EGTLASSLAV IEKIHQNFFS HTSLDEVDVR NILASEQRKI
1160 1170 1180 1190 1200
LAGCRIVFSR IIPVGEAKPH LHPLWQTAEQ FGAVCTTQVD EHVTHVVTNS
1210 1220 1230 1240
LGTDKVNWAL TRGRFVVHPG WVEASAFLYQ RANENLYAIN P
Length:1,241
Mass (Da):136,479
Last modified:May 26, 2009 - v2
Checksum:iCEFFBD725719D404
GO

Sequence cautioni

The sequence AAB80671 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1010A → V in AAM94371 (PubMed:12149434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486633 mRNA Translation: AAM94371.1
AC002332 Genomic DNA Translation: AAB80671.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC08850.1
PIRiG84746
RefSeqiNP_180912.2, NM_128914.3
UniGeneiAt.43643

Genome annotation databases

EnsemblPlantsiAT2G33540.1; AT2G33540.1; AT2G33540
GeneIDi817919
GrameneiAT2G33540.1; AT2G33540.1; AT2G33540
KEGGiath:AT2G33540

Similar proteinsi

Entry informationi

Entry nameiCPL3_ARATH
AccessioniPrimary (citable) accession number: Q8LL04
Secondary accession number(s): O22804
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: April 25, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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