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Protein

Long chain acyl-CoA synthetase 7, peroxisomal

Gene

LACS7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate. Displays redundant function with LACS7 into the seed development process (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

Cofactori

Mg2+By similarity

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi266 – 277ATPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • fatty acid metabolic process Source: UniProtKB
  • response to ozone Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G27600-MONOMER
MetaCyc:AT5G27600-MONOMER
ReactomeiR-ATH-2046105 Linoleic acid (LA) metabolism
R-ATH-2046106 alpha-linolenic acid (ALA) metabolism
R-ATH-75876 Synthesis of very long-chain fatty acyl-CoAs
UniPathwayiUPA00199

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 7, peroxisomal (EC:6.2.1.3)
Gene namesi
Name:LACS7
Ordered Locus Names:At5g27600
ORF Names:F15A18.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G27600
TAIRilocus:2143661 AT5G27600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids into beta-oxidation, a necessary process to pursue the development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004014161 – 700Long chain acyl-CoA synthetase 7, peroxisomalAdd BLAST700

Proteomic databases

PaxDbiQ8LKS5
PRIDEiQ8LKS5

Expressioni

Tissue specificityi

Preferentially expressed in seeds.1 Publication

Developmental stagei

Induced during seed germination.1 Publication

Inductioni

Up-regulated by ozone and salt.1 Publication

Gene expression databases

ExpressionAtlasiQ8LKS5 baseline and differential
GenevisibleiQ8LKS5 AT

Interactioni

Subunit structurei

Interacts with PEX5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX5Q9FMA34EBI-993851,EBI-993861

Protein-protein interaction databases

BioGridi18094, 2 interactors
IntActiQ8LKS5, 1 interactor
STRINGi3702.AT5G27600.1

Structurei

3D structure databases

ProteinModelPortaliQ8LKS5
SMRiQ8LKS5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni526 – 550Fatty acid-bindingSequence analysisAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 18Microbody targeting signalSequence analysis9
Motifi698 – 700Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
HOGENOMiHOG000159459
InParanoidiQ8LKS5
KOiK01897
OMAiEELNYWA
OrthoDBiEOG0936045E
PhylomeDBiQ8LKS5

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q8LKS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFASPEQRR LETIRSHIDT SPTNDQSSSL FLNATASSAS PFFKEDSYSV
60 70 80 90 100
VLPEKLDTGK WNVYRSKRSP TKLVSRFPDH PEIGTLHDNF VHAVETYAEN
110 120 130 140 150
KYLGTRVRSD GTIGEYSWMT YGEAASERQA IGSGLLFHGV NQGDCVGLYF
160 170 180 190 200
INRPEWLVVD HACAAYSFVS VPLYDTLGPD AVKFVVNHAN LQAIFCVPQT
210 220 230 240 250
LNILLSFLAE IPSIRLIVVV GGADEHLPSL PRGTGVTIVS YQKLLSQGRS
260 270 280 290 300
SLHPFSPPKP EDIATICYTS GTTGTPKGVV LTHGNLIANV AGSSVEAEFF
310 320 330 340 350
PSDVYISYLP LAHIYERANQ IMGVYGGVAV GFYQGDVFKL MDDFAVLRPT
360 370 380 390 400
IFCSVPRLYN RIYDGITSAV KSSGVVKKRL FEIAYNSKKQ AIINGRTPSA
410 420 430 440 450
FWDKLVFNKI KEKLGGRVRF MGSGASPLSP DVMDFLRICF GCSVREGYGM
460 470 480 490 500
TETSCVISAM DDGDNLSGHV GSPNPACEVK LVDVPEMNYT SDDQPYPRGE
510 520 530 540 550
ICVRGPIIFK GYYKDEEQTR EILDGDGWLH TGDIGLWLPG GRLKIIDRKK
560 570 580 590 600
NIFKLAQGEY IAPEKIENVY TKCRFVSQCF IHGDSFNSSL VAIVSVDPEV
610 620 630 640 650
MKDWAASEGI KYEHLGQLCN DPRVRKTVLA EMDDLGREAQ LRGFEFAKAV
660 670 680 690 700
TLVPEPFTLE NGLLTPTFKI KRPQAKAYFA EAISKMYAEI AASNPIPSKL
Length:700
Mass (Da):77,353
Last modified:November 30, 2010 - v2
Checksum:iEAD95B9F50DA75DB
GO

Sequence cautioni

The sequence BAC43213 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti657F → L in AAM28874 (PubMed:12177484).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503757 mRNA Translation: AAM28874.1
AC007478 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93704.1
AK118614 mRNA Translation: BAC43213.1 Different initiation.
RefSeqiNP_198112.2, NM_122642.5
UniGeneiAt.20193

Genome annotation databases

EnsemblPlantsiAT5G27600.1; AT5G27600.1; AT5G27600
GeneIDi832820
GrameneiAT5G27600.1; AT5G27600.1; AT5G27600
KEGGiath:AT5G27600

Similar proteinsi

Entry informationi

Entry nameiLACS7_ARATH
AccessioniPrimary (citable) accession number: Q8LKS5
Secondary accession number(s): Q8GWV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 23, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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