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Q8LKS5

- LACS7_ARATH

UniProt

Q8LKS5 - LACS7_ARATH

Protein

Long chain acyl-CoA synthetase 7, peroxisomal

Gene

LACS7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate. Displays redundant function with LACS7 into the seed development process By similarity.By similarity

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi266 – 27712ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
    3. protein binding Source: IntAct

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB
    2. long-chain fatty acid metabolic process Source: GOC
    3. response to ozone Source: TAIR
    4. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G27600-MONOMER.
    MetaCyc:AT5G27600-MONOMER.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 7, peroxisomal (EC:6.2.1.3)
    Gene namesi
    Name:LACS7
    Ordered Locus Names:At5g27600
    ORF Names:F15A18.60
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G27600.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Disruption phenotypei

    Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids into beta-oxidation, a necessary process to pursue the development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 700700Long chain acyl-CoA synthetase 7, peroxisomalPRO_0000401416Add
    BLAST

    Proteomic databases

    PaxDbiQ8LKS5.
    PRIDEiQ8LKS5.

    Expressioni

    Tissue specificityi

    Preferentially expressed in seeds.1 Publication

    Developmental stagei

    Induced during seed germination.1 Publication

    Inductioni

    Up-regulated by ozone and salt.1 Publication

    Gene expression databases

    GenevestigatoriQ8LKS5.

    Interactioni

    Subunit structurei

    Interacts with PEX5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEX5Q9FMA34EBI-993851,EBI-993861

    Protein-protein interaction databases

    BioGridi18094. 1 interaction.
    IntActiQ8LKS5. 1 interaction.
    STRINGi3702.AT5G27600.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LKS5.
    SMRiQ8LKS5. Positions 75-693.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni526 – 55025Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi10 – 189Microbody targeting signalSequence Analysis
    Motifi698 – 7003Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiQ8LKS5.
    KOiK01897.
    OMAiYEELCQN.
    PhylomeDBiQ8LKS5.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8LKS5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFASPEQRR LETIRSHIDT SPTNDQSSSL FLNATASSAS PFFKEDSYSV    50
    VLPEKLDTGK WNVYRSKRSP TKLVSRFPDH PEIGTLHDNF VHAVETYAEN 100
    KYLGTRVRSD GTIGEYSWMT YGEAASERQA IGSGLLFHGV NQGDCVGLYF 150
    INRPEWLVVD HACAAYSFVS VPLYDTLGPD AVKFVVNHAN LQAIFCVPQT 200
    LNILLSFLAE IPSIRLIVVV GGADEHLPSL PRGTGVTIVS YQKLLSQGRS 250
    SLHPFSPPKP EDIATICYTS GTTGTPKGVV LTHGNLIANV AGSSVEAEFF 300
    PSDVYISYLP LAHIYERANQ IMGVYGGVAV GFYQGDVFKL MDDFAVLRPT 350
    IFCSVPRLYN RIYDGITSAV KSSGVVKKRL FEIAYNSKKQ AIINGRTPSA 400
    FWDKLVFNKI KEKLGGRVRF MGSGASPLSP DVMDFLRICF GCSVREGYGM 450
    TETSCVISAM DDGDNLSGHV GSPNPACEVK LVDVPEMNYT SDDQPYPRGE 500
    ICVRGPIIFK GYYKDEEQTR EILDGDGWLH TGDIGLWLPG GRLKIIDRKK 550
    NIFKLAQGEY IAPEKIENVY TKCRFVSQCF IHGDSFNSSL VAIVSVDPEV 600
    MKDWAASEGI KYEHLGQLCN DPRVRKTVLA EMDDLGREAQ LRGFEFAKAV 650
    TLVPEPFTLE NGLLTPTFKI KRPQAKAYFA EAISKMYAEI AASNPIPSKL 700
    Length:700
    Mass (Da):77,353
    Last modified:November 30, 2010 - v2
    Checksum:iEAD95B9F50DA75DB
    GO

    Sequence cautioni

    The sequence BAC43213.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti657 – 6571F → L in AAM28874. (PubMed:12177484)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503757 mRNA. Translation: AAM28874.1.
    AC007478 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93704.1.
    AK118614 mRNA. Translation: BAC43213.1. Different initiation.
    RefSeqiNP_198112.2. NM_122642.4.
    UniGeneiAt.20193.

    Genome annotation databases

    EnsemblPlantsiAT5G27600.1; AT5G27600.1; AT5G27600.
    GeneIDi832820.
    KEGGiath:AT5G27600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503757 mRNA. Translation: AAM28874.1 .
    AC007478 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93704.1 .
    AK118614 mRNA. Translation: BAC43213.1 . Different initiation.
    RefSeqi NP_198112.2. NM_122642.4.
    UniGenei At.20193.

    3D structure databases

    ProteinModelPortali Q8LKS5.
    SMRi Q8LKS5. Positions 75-693.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18094. 1 interaction.
    IntActi Q8LKS5. 1 interaction.
    STRINGi 3702.AT5G27600.1-P.

    Proteomic databases

    PaxDbi Q8LKS5.
    PRIDEi Q8LKS5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G27600.1 ; AT5G27600.1 ; AT5G27600 .
    GeneIDi 832820.
    KEGGi ath:AT5G27600.

    Organism-specific databases

    TAIRi AT5G27600.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi Q8LKS5.
    KOi K01897.
    OMAi YEELCQN.
    PhylomeDBi Q8LKS5.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT5G27600-MONOMER.
    MetaCyc:AT5G27600-MONOMER.

    Gene expression databases

    Genevestigatori Q8LKS5.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-700.
      Strain: cv. Columbia.
    5. "Two long-chain acyl-CoA synthetases from Arabidopsis thaliana involved in peroxisomal fatty acid beta-oxidation."
      Fulda M., Shockey J., Werber M., Wolter F.P., Heinz E.
      Plant J. 32:93-103(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, ENZYME ACTIVITY.
    6. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.
    7. "Peroxisomal Acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana."
      Fulda M., Schnurr J., Abbadi A., Heinz E., Browse J.
      Plant Cell 16:394-405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    8. "AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5."
      Bonsegna S., Slocombe S.P., De Bellis L., Baker A.
      Arch. Biochem. Biophys. 443:74-81(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PEX5, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiLACS7_ARATH
    AccessioniPrimary (citable) accession number: Q8LKS5
    Secondary accession number(s): Q8GWV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3