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Protein

Long chain acyl-CoA synthetase 7, peroxisomal

Gene

LACS7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate. Displays redundant function with LACS7 into the seed development process (By similarity).By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

Cofactori

Mg2+By similarity

Pathway:ifatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi266 – 27712ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • long-chain fatty acid-CoA ligase activity Source: UniProtKB

GO - Biological processi

  • fatty acid metabolic process Source: UniProtKB
  • long-chain fatty acid metabolic process Source: GOC
  • response to ozone Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G27600-MONOMER.
MetaCyc:AT5G27600-MONOMER.
ReactomeiREACT_277605. Linoleic acid (LA) metabolism.
REACT_312910. Synthesis of very long-chain fatty acyl-CoAs.
REACT_344015. alpha-linolenic acid (ALA) metabolism.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 7, peroxisomal (EC:6.2.1.3)
Gene namesi
Name:LACS7
Ordered Locus Names:At5g27600
ORF Names:F15A18.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G27600.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids into beta-oxidation, a necessary process to pursue the development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700Long chain acyl-CoA synthetase 7, peroxisomalPRO_0000401416Add
BLAST

Proteomic databases

PaxDbiQ8LKS5.
PRIDEiQ8LKS5.

Expressioni

Tissue specificityi

Preferentially expressed in seeds.1 Publication

Developmental stagei

Induced during seed germination.1 Publication

Inductioni

Up-regulated by ozone and salt.1 Publication

Interactioni

Subunit structurei

Interacts with PEX5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX5Q9FMA34EBI-993851,EBI-993861

Protein-protein interaction databases

BioGridi18094. 2 interactions.
IntActiQ8LKS5. 1 interaction.
STRINGi3702.AT5G27600.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LKS5.
SMRiQ8LKS5. Positions 75-693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni526 – 55025Fatty acid-bindingSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 189Microbody targeting signalSequence Analysis
Motifi698 – 7003Microbody targeting signalSequence Analysis

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ8LKS5.
KOiK01897.
OMAiAYFAEAI.
PhylomeDBiQ8LKS5.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LKS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFASPEQRR LETIRSHIDT SPTNDQSSSL FLNATASSAS PFFKEDSYSV
60 70 80 90 100
VLPEKLDTGK WNVYRSKRSP TKLVSRFPDH PEIGTLHDNF VHAVETYAEN
110 120 130 140 150
KYLGTRVRSD GTIGEYSWMT YGEAASERQA IGSGLLFHGV NQGDCVGLYF
160 170 180 190 200
INRPEWLVVD HACAAYSFVS VPLYDTLGPD AVKFVVNHAN LQAIFCVPQT
210 220 230 240 250
LNILLSFLAE IPSIRLIVVV GGADEHLPSL PRGTGVTIVS YQKLLSQGRS
260 270 280 290 300
SLHPFSPPKP EDIATICYTS GTTGTPKGVV LTHGNLIANV AGSSVEAEFF
310 320 330 340 350
PSDVYISYLP LAHIYERANQ IMGVYGGVAV GFYQGDVFKL MDDFAVLRPT
360 370 380 390 400
IFCSVPRLYN RIYDGITSAV KSSGVVKKRL FEIAYNSKKQ AIINGRTPSA
410 420 430 440 450
FWDKLVFNKI KEKLGGRVRF MGSGASPLSP DVMDFLRICF GCSVREGYGM
460 470 480 490 500
TETSCVISAM DDGDNLSGHV GSPNPACEVK LVDVPEMNYT SDDQPYPRGE
510 520 530 540 550
ICVRGPIIFK GYYKDEEQTR EILDGDGWLH TGDIGLWLPG GRLKIIDRKK
560 570 580 590 600
NIFKLAQGEY IAPEKIENVY TKCRFVSQCF IHGDSFNSSL VAIVSVDPEV
610 620 630 640 650
MKDWAASEGI KYEHLGQLCN DPRVRKTVLA EMDDLGREAQ LRGFEFAKAV
660 670 680 690 700
TLVPEPFTLE NGLLTPTFKI KRPQAKAYFA EAISKMYAEI AASNPIPSKL
Length:700
Mass (Da):77,353
Last modified:November 30, 2010 - v2
Checksum:iEAD95B9F50DA75DB
GO

Sequence cautioni

The sequence BAC43213.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti657 – 6571F → L in AAM28874 (PubMed:12177484).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503757 mRNA. Translation: AAM28874.1.
AC007478 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93704.1.
AK118614 mRNA. Translation: BAC43213.1. Different initiation.
RefSeqiNP_198112.2. NM_122642.4.
UniGeneiAt.20193.

Genome annotation databases

EnsemblPlantsiAT5G27600.1; AT5G27600.1; AT5G27600.
GeneIDi832820.
KEGGiath:AT5G27600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503757 mRNA. Translation: AAM28874.1.
AC007478 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93704.1.
AK118614 mRNA. Translation: BAC43213.1. Different initiation.
RefSeqiNP_198112.2. NM_122642.4.
UniGeneiAt.20193.

3D structure databases

ProteinModelPortaliQ8LKS5.
SMRiQ8LKS5. Positions 75-693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18094. 2 interactions.
IntActiQ8LKS5. 1 interaction.
STRINGi3702.AT5G27600.1.

Proteomic databases

PaxDbiQ8LKS5.
PRIDEiQ8LKS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G27600.1; AT5G27600.1; AT5G27600.
GeneIDi832820.
KEGGiath:AT5G27600.

Organism-specific databases

TAIRiAT5G27600.

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ8LKS5.
KOiK01897.
OMAiAYFAEAI.
PhylomeDBiQ8LKS5.

Enzyme and pathway databases

UniPathwayiUPA00199.
BioCyciARA:AT5G27600-MONOMER.
MetaCyc:AT5G27600-MONOMER.
ReactomeiREACT_277605. Linoleic acid (LA) metabolism.
REACT_312910. Synthesis of very long-chain fatty acyl-CoAs.
REACT_344015. alpha-linolenic acid (ALA) metabolism.

Miscellaneous databases

PROiQ8LKS5.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-700.
    Strain: cv. Columbia.
  5. "Two long-chain acyl-CoA synthetases from Arabidopsis thaliana involved in peroxisomal fatty acid beta-oxidation."
    Fulda M., Shockey J., Werber M., Wolter F.P., Heinz E.
    Plant J. 32:93-103(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, ENZYME ACTIVITY.
  6. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.
  7. "Peroxisomal Acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana."
    Fulda M., Schnurr J., Abbadi A., Heinz E., Browse J.
    Plant Cell 16:394-405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  8. "AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5."
    Bonsegna S., Slocombe S.P., De Bellis L., Baker A.
    Arch. Biochem. Biophys. 443:74-81(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX5, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiLACS7_ARATH
AccessioniPrimary (citable) accession number: Q8LKS5
Secondary accession number(s): Q8GWV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: July 22, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.