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Q8LKS5

- LACS7_ARATH

UniProt

Q8LKS5 - LACS7_ARATH

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Protein

Long chain acyl-CoA synthetase 7, peroxisomal

Gene
LACS7, At5g27600, F15A18.60
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate. Displays redundant function with LACS7 into the seed development process By similarity.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.2 Publications

Cofactori

Magnesium By similarity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi266 – 27712ATP Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
  3. protein binding Source: IntAct

GO - Biological processi

  1. fatty acid metabolic process Source: UniProtKB
  2. long-chain fatty acid metabolic process Source: GOC
  3. response to ozone Source: TAIR
  4. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G27600-MONOMER.
MetaCyc:AT5G27600-MONOMER.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 7, peroxisomal (EC:6.2.1.3)
Gene namesi
Name:LACS7
Ordered Locus Names:At5g27600
ORF Names:F15A18.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G27600.

Subcellular locationi

Peroxisome 1 Publication

GO - Cellular componenti

  1. peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids into beta-oxidation, a necessary process to pursue the development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700Long chain acyl-CoA synthetase 7, peroxisomalPRO_0000401416Add
BLAST

Proteomic databases

PaxDbiQ8LKS5.
PRIDEiQ8LKS5.

Expressioni

Tissue specificityi

Preferentially expressed in seeds.1 Publication

Developmental stagei

Induced during seed germination.1 Publication

Inductioni

Up-regulated by ozone and salt.1 Publication

Gene expression databases

GenevestigatoriQ8LKS5.

Interactioni

Subunit structurei

Interacts with PEX5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX5Q9FMA34EBI-993851,EBI-993861

Protein-protein interaction databases

BioGridi18094. 1 interaction.
IntActiQ8LKS5. 1 interaction.
STRINGi3702.AT5G27600.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LKS5.
SMRiQ8LKS5. Positions 75-693.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni526 – 55025Fatty acid-binding Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 189Microbody targeting signal Reviewed prediction
Motifi698 – 7003Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiQ8LKS5.
KOiK01897.
OMAiYEELCQN.
PhylomeDBiQ8LKS5.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LKS5-1 [UniParc]FASTAAdd to Basket

« Hide

MEFASPEQRR LETIRSHIDT SPTNDQSSSL FLNATASSAS PFFKEDSYSV    50
VLPEKLDTGK WNVYRSKRSP TKLVSRFPDH PEIGTLHDNF VHAVETYAEN 100
KYLGTRVRSD GTIGEYSWMT YGEAASERQA IGSGLLFHGV NQGDCVGLYF 150
INRPEWLVVD HACAAYSFVS VPLYDTLGPD AVKFVVNHAN LQAIFCVPQT 200
LNILLSFLAE IPSIRLIVVV GGADEHLPSL PRGTGVTIVS YQKLLSQGRS 250
SLHPFSPPKP EDIATICYTS GTTGTPKGVV LTHGNLIANV AGSSVEAEFF 300
PSDVYISYLP LAHIYERANQ IMGVYGGVAV GFYQGDVFKL MDDFAVLRPT 350
IFCSVPRLYN RIYDGITSAV KSSGVVKKRL FEIAYNSKKQ AIINGRTPSA 400
FWDKLVFNKI KEKLGGRVRF MGSGASPLSP DVMDFLRICF GCSVREGYGM 450
TETSCVISAM DDGDNLSGHV GSPNPACEVK LVDVPEMNYT SDDQPYPRGE 500
ICVRGPIIFK GYYKDEEQTR EILDGDGWLH TGDIGLWLPG GRLKIIDRKK 550
NIFKLAQGEY IAPEKIENVY TKCRFVSQCF IHGDSFNSSL VAIVSVDPEV 600
MKDWAASEGI KYEHLGQLCN DPRVRKTVLA EMDDLGREAQ LRGFEFAKAV 650
TLVPEPFTLE NGLLTPTFKI KRPQAKAYFA EAISKMYAEI AASNPIPSKL 700
Length:700
Mass (Da):77,353
Last modified:November 30, 2010 - v2
Checksum:iEAD95B9F50DA75DB
GO

Sequence cautioni

The sequence BAC43213.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti657 – 6571F → L in AAM28874. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF503757 mRNA. Translation: AAM28874.1.
AC007478 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93704.1.
AK118614 mRNA. Translation: BAC43213.1. Different initiation.
RefSeqiNP_198112.2. NM_122642.4.
UniGeneiAt.20193.

Genome annotation databases

EnsemblPlantsiAT5G27600.1; AT5G27600.1; AT5G27600.
GeneIDi832820.
KEGGiath:AT5G27600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF503757 mRNA. Translation: AAM28874.1 .
AC007478 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93704.1 .
AK118614 mRNA. Translation: BAC43213.1 . Different initiation.
RefSeqi NP_198112.2. NM_122642.4.
UniGenei At.20193.

3D structure databases

ProteinModelPortali Q8LKS5.
SMRi Q8LKS5. Positions 75-693.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18094. 1 interaction.
IntActi Q8LKS5. 1 interaction.
STRINGi 3702.AT5G27600.1-P.

Proteomic databases

PaxDbi Q8LKS5.
PRIDEi Q8LKS5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G27600.1 ; AT5G27600.1 ; AT5G27600 .
GeneIDi 832820.
KEGGi ath:AT5G27600.

Organism-specific databases

TAIRi AT5G27600.

Phylogenomic databases

eggNOGi COG1022.
HOGENOMi HOG000159459.
InParanoidi Q8LKS5.
KOi K01897.
OMAi YEELCQN.
PhylomeDBi Q8LKS5.

Enzyme and pathway databases

UniPathwayi UPA00199 .
BioCyci ARA:AT5G27600-MONOMER.
MetaCyc:AT5G27600-MONOMER.

Gene expression databases

Genevestigatori Q8LKS5.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 604-700.
    Strain: cv. Columbia.
  5. "Two long-chain acyl-CoA synthetases from Arabidopsis thaliana involved in peroxisomal fatty acid beta-oxidation."
    Fulda M., Shockey J., Werber M., Wolter F.P., Heinz E.
    Plant J. 32:93-103(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, ENZYME ACTIVITY.
  6. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.
  7. "Peroxisomal Acyl-CoA synthetase activity is essential for seedling development in Arabidopsis thaliana."
    Fulda M., Schnurr J., Abbadi A., Heinz E., Browse J.
    Plant Cell 16:394-405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  8. "AtLACS7 interacts with the TPR domains of the PTS1 receptor PEX5."
    Bonsegna S., Slocombe S.P., De Bellis L., Baker A.
    Arch. Biochem. Biophys. 443:74-81(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PEX5, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiLACS7_ARATH
AccessioniPrimary (citable) accession number: Q8LKS5
Secondary accession number(s): Q8GWV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 30, 2010
Last modified: June 11, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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