Q8LK56 (DME_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator DEMETER
EC=3.2.2.-

Alternative name(s):
DNA glycosylase-related protein DME

Gene names

Name:	DME
Ordered Locus Names:	At5g04560/At5g04570/At5g04580
ORF Names:	T32M21.160/T32M21.170/T32M21.180

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1987 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator involved in gene imprinting. Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Allows the expression of the maternal copy of the imprinted MEA gene before fertilization, possibly by antagonizing or suppressing DNA methylation on target promoter. Probably acts by nicking the MEA promoter. Required for stable reproducible patterns of floral and vegetative development. Ref.1 Ref.2 Ref.7
Cofactor	Binds 1 4Fe-4S cluster. The cluster is probably involved in the proper positioning of the protein along the DNA strand By similarity.
Subcellular location	Nucleus.
Tissue specificity	Mainly expressed in immature flower buds, then decreases as the flower matures. Expressed in the ovule carpels, but not expressed in pollen stamens. Expressed in developing and mature ovules (stages 12-14), then strongly decreases after fertilization.
Developmental stage	Maternally expressed. Expressed primarily in the central cell of gametophyte before fertilization. Not expressed in endosperm and embryo after fertilization.
Domain	The DEMETER domain, which is present in proteins of the subfamily, is related to the J-domain, but lacks some important conserved residues.
Miscellaneous	Although strongly related to DNA glycosylase proteins, it differs from these proteins because of its large size and its unique N-terminal basic domain. The DNA repair function has not been proved and may not exist.
Sequence similarities	Belongs to the DNA glycosylase family. DEMETER subfamily.
Sequence caution	The sequence CAB85562.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to three different genes At5g04560, At5g04570 and At5g04580. The sequence CAB85563.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to three different genes At5g04560, At5g04570 and At5g04580. The sequence CAB85564.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to three different genes At5g04560, At5g04570 and At5g04580.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	4Fe-4S DNA-binding Iron Iron-sulfur Metal-binding
Molecular function	Activator Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA methylation Inferred from direct assay Ref.2. Source: TAIR base-excision repair Inferred from electronic annotation. Source: InterPro embryo development ending in seed dormancy Inferred from mutant phenotype Ref.1. Source: TAIR regulation of gene expression by genetic imprinting Inferred from mutant phenotype PubMed 16469697. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	polar nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW DNA N-glycosylase activity Inferred from direct assay Ref.2. Source: TAIR DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-(apurinic or apyrimidinic site) lyase activity Inferred from direct assay Ref.2. Source: TAIR endonuclease activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: Q8LK56-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q8LK56-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-258: Missing.

Isoform 2 (identifier: Q8LK56-2)

The sequence of this isoform differs from the canonical sequence as follows:
     259-1571: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1987	1987	Transcriptional activator DEMETER		PRO_0000102245
Regions
	Region	955 – 1054	100	DEMETER
	Compositional bias	291 – 366	76	Lys-rich (basic)
	Compositional bias	473 – 625	153	Gln-rich
Sites
	Metal binding	1629	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1636	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1639	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1645	1	Iron-sulfur (4Fe-4S) By similarity
Natural variations
	Alternative sequence	1 – 258	258	Missing in isoform 1.		VSP_019283
	Alternative sequence	259 – 1571	1313	Missing in isoform 2.		VSP_007455
Experimental info
	Mutagenesis	1562	1	D → N: Loss of activity and abnormal MEA imprinting. Ref.7
	Sequence conflict	1679	1	Y → F in AAM77215. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: 4035C0D344DCF375	FASTA	1,987	221,147
	10 20 30 40 50 60 MNSRADPGDR YFRVPLENQT QQEFMGSWIP FTPKKPRSSL MVDERVINQD LNGFPGGEFV 70 80 90 100 110 120 DRGFCNTGVD HNGVFDHGAH QGVTNLSMMI NSLAGSHAQA WSNSERDLLG RSEVTSPLAP 130 140 150 160 170 180 VIRNTTGNVE PVNGNFTSDV GMVNGPFTQS GTSQAGYNEF ELDDLLNPDQ MPFSFTSLLS 190 200 210 220 230 240 GGDSLFKVRQ YGPPACNKPL YNLNSPIRRE AVGSVCESSF QYVPSTPSLF RTGEKTGFLE 250 260 270 280 290 300 QIVTTTGHEI PEPKSDKSMQ SIMDSSAVNA TEATEQNDGS RQDVLEFDLN KTPQQKPSKR 310 320 330 340 350 360 KRKFMPKVVV EGKPKRKPRK PAELPKVVVE GKPKRKPRKA ATQEKVKSKE TGSAKKKNLK 370 380 390 400 410 420 ESATKKPANV GDMSNKSPEV TLKSCRKALN FDLENPGDAR QGDSESEIVQ NSSGANSFSE 430 440 450 460 470 480 IRDAIGGTNG SFLDSVSQID KTNGLGAMNQ PLEVSMGNQP DKLSTGAKLA RDQQPDLLTR 490 500 510 520 530 540 NQQCQFPVAT QNTQFPMENQ QAWLQMKNQL IGFPFGNQQP RMTIRNQQPC LAMGNQQPMY 550 560 570 580 590 600 LIGTPRPALV SGNQQLGGPQ GNKRPIFLNH QTCLPAGNQL YGSPTDMHQL VMSTGGQQHG 610 620 630 640 650 660 LLIKNQQPGS LIRGQQPCVP LIDQQPATPK GFTHLNQMVA TSMSSPGLRP HSQSQVPTTY 670 680 690 700 710 720 LHVESVSRIL NGTTGTCQRS RAPAYDSLQQ DIHQGNKYIL SHEISNGNGC KKALPQNSSL 730 740 750 760 770 780 PTPIMAKLEE ARGSKRQYHR AMGQTEKHDL NLAQQIAQSQ DVERHNSSTC VEYLDAAKKT 790 800 810 820 830 840 KIQKVVQENL HGMPPEVIEI EDDPTDGARK GKNTASISKG ASKGNSSPVK KTAEKEKCIV 850 860 870 880 890 900 PKTPAKKGRA GRKKSVPPPA HASEIQLWQP TPPKTPLSRS KPKGKGRKSI QDSGKARGPS 910 920 930 940 950 960 GELLCQDSIA EIIYRMQNLY LGDKEREQEQ NAMVLYKGDG ALVPYESKKR KPRPKVDIDD 970 980 990 1000 1010 1020 ETTRIWNLLM GKGDEKEGDE EKDKKKEKWW EEERRVFRGR ADSFIARMHL VQGDRRFSPW 1030 1040 1050 1060 1070 1080 KGSVVDSVIG VFLTQNVSDH LSSSAFMSLA ARFPPKLSSS REDERNVRSV VVEDPEGCIL 1090 1100 1110 1120 1130 1140 NLNEIPSWQE KVQHPSDMEV SGVDSGSKEQ LRDCSNSGIE RFNFLEKSIQ NLEEEVLSSQ 1150 1160 1170 1180 1190 1200 DSFDPAIFQS CGRVGSCSCS KSDAEFPTTR CETKTVSGTS QSVQTGSPNL SDEICLQGNE 1210 1220 1230 1240 1250 1260 RPHLYEGSGD VQKQETTNVA QKKPDLEKTM NWKDSVCFGQ PRNDTNWQTT PSSSYEQCAT 1270 1280 1290 1300 1310 1320 RQPHVLDIED FGMQGEGLGY SWMSISPRVD RVKNKNVPRR FFRQGGSVPR EFTGQIIPST 1330 1340 1350 1360 1370 1380 PHELPGMGLS GSSSAVQEHQ DDTQHNQQDE MNKASHLQKT FLDLLNSSEE CLTRQSSTKQ 1390 1400 1410 1420 1430 1440 NITDGCLPRD RTAEDVVDPL SNNSSLQNIL VESNSSNKEQ TAVEYKETNA TILREMKGTL 1450 1460 1470 1480 1490 1500 ADGKKPTSQW DSLRKDVEGN EGRQERNKNN MDSIDYEAIR RASISEISEA IKERGMNNML 1510 1520 1530 1540 1550 1560 AVRIKDFLER IVKDHGGIDL EWLRESPPDK AKDYLLSIRG LGLKSVECVR LLTLHNLAFP 1570 1580 1590 1600 1610 1620 VDTNVGRIAV RMGWVPLQPL PESLQLHLLE LYPVLESIQK FLWPRLCKLD QRTLYELHYQ 1630 1640 1650 1660 1670 1680 LITFGKVFCT KSRPNCNACP MRGECRHFAS AYASARLALP APEERSLTSA TIPVPPESYP 1690 1700 1710 1720 1730 1740 PVAIPMIELP LPLEKSLASG APSNRENCEP IIEEPASPGQ ECTEITESDI EDAYYNEDPD 1750 1760 1770 1780 1790 1800 EIPTIKLNIE QFGMTLREHM ERNMELQEGD MSKALVALHP TTTSIPTPKL KNISRLRTEH 1810 1820 1830 1840 1850 1860 QVYELPDSHR LLDGMDKREP DDPSPYLLAI WTPGETANSA QPPEQKCGGK ASGKMCFDET 1870 1880 1890 1900 1910 1920 CSECNSLREA NSQTVRGTLL IPCRTAMRGS FPLNGTYFQV NELFADHESS LKPIDVPRDW 1930 1940 1950 1960 1970 1980 IWDLPRRTVY FGTSVTSIFR GLSTEQIQFC FWKGFVCVRG FEQKTRAPRP LMARLHFPAS KLKNNKT
	Isoform 1 [UniParc]. Checksum: D4A3EA9C65AD1FE2	FASTA	1,729	192,905
	10 20 30 40 50 60 MQSIMDSSAV NATEATEQND GSRQDVLEFD LNKTPQQKPS KRKRKFMPKV VVEGKPKRKP 70 80 90 100 110 120 RKPAELPKVV VEGKPKRKPR KAATQEKVKS KETGSAKKKN LKESATKKPA NVGDMSNKSP 130 140 150 160 170 180 EVTLKSCRKA LNFDLENPGD ARQGDSESEI VQNSSGANSF SEIRDAIGGT NGSFLDSVSQ 190 200 210 220 230 240 IDKTNGLGAM NQPLEVSMGN QPDKLSTGAK LARDQQPDLL TRNQQCQFPV ATQNTQFPME 250 260 270 280 290 300 NQQAWLQMKN QLIGFPFGNQ QPRMTIRNQQ PCLAMGNQQP MYLIGTPRPA LVSGNQQLGG 310 320 330 340 350 360 PQGNKRPIFL NHQTCLPAGN QLYGSPTDMH QLVMSTGGQQ HGLLIKNQQP GSLIRGQQPC 370 380 390 400 410 420 VPLIDQQPAT PKGFTHLNQM VATSMSSPGL RPHSQSQVPT TYLHVESVSR ILNGTTGTCQ 430 440 450 460 470 480 RSRAPAYDSL QQDIHQGNKY ILSHEISNGN GCKKALPQNS SLPTPIMAKL EEARGSKRQY 490 500 510 520 530 540 HRAMGQTEKH DLNLAQQIAQ SQDVERHNSS TCVEYLDAAK KTKIQKVVQE NLHGMPPEVI 550 560 570 580 590 600 EIEDDPTDGA RKGKNTASIS KGASKGNSSP VKKTAEKEKC IVPKTPAKKG RAGRKKSVPP 610 620 630 640 650 660 PAHASEIQLW QPTPPKTPLS RSKPKGKGRK SIQDSGKARG PSGELLCQDS IAEIIYRMQN 670 680 690 700 710 720 LYLGDKEREQ EQNAMVLYKG DGALVPYESK KRKPRPKVDI DDETTRIWNL LMGKGDEKEG 730 740 750 760 770 780 DEEKDKKKEK WWEEERRVFR GRADSFIARM HLVQGDRRFS PWKGSVVDSV IGVFLTQNVS 790 800 810 820 830 840 DHLSSSAFMS LAARFPPKLS SSREDERNVR SVVVEDPEGC ILNLNEIPSW QEKVQHPSDM 850 860 870 880 890 900 EVSGVDSGSK EQLRDCSNSG IERFNFLEKS IQNLEEEVLS SQDSFDPAIF QSCGRVGSCS 910 920 930 940 950 960 CSKSDAEFPT TRCETKTVSG TSQSVQTGSP NLSDEICLQG NERPHLYEGS GDVQKQETTN 970 980 990 1000 1010 1020 VAQKKPDLEK TMNWKDSVCF GQPRNDTNWQ TTPSSSYEQC ATRQPHVLDI EDFGMQGEGL 1030 1040 1050 1060 1070 1080 GYSWMSISPR VDRVKNKNVP RRFFRQGGSV PREFTGQIIP STPHELPGMG LSGSSSAVQE 1090 1100 1110 1120 1130 1140 HQDDTQHNQQ DEMNKASHLQ KTFLDLLNSS EECLTRQSST KQNITDGCLP RDRTAEDVVD 1150 1160 1170 1180 1190 1200 PLSNNSSLQN ILVESNSSNK EQTAVEYKET NATILREMKG TLADGKKPTS QWDSLRKDVE 1210 1220 1230 1240 1250 1260 GNEGRQERNK NNMDSIDYEA IRRASISEIS EAIKERGMNN MLAVRIKDFL ERIVKDHGGI 1270 1280 1290 1300 1310 1320 DLEWLRESPP DKAKDYLLSI RGLGLKSVEC VRLLTLHNLA FPVDTNVGRI AVRMGWVPLQ 1330 1340 1350 1360 1370 1380 PLPESLQLHL LELYPVLESI QKFLWPRLCK LDQRTLYELH YQLITFGKVF CTKSRPNCNA 1390 1400 1410 1420 1430 1440 CPMRGECRHF ASAYASARLA LPAPEERSLT SATIPVPPES YPPVAIPMIE LPLPLEKSLA 1450 1460 1470 1480 1490 1500 SGAPSNRENC EPIIEEPASP GQECTEITES DIEDAYYNED PDEIPTIKLN IEQFGMTLRE 1510 1520 1530 1540 1550 1560 HMERNMELQE GDMSKALVAL HPTTTSIPTP KLKNISRLRT EHQVYELPDS HRLLDGMDKR 1570 1580 1590 1600 1610 1620 EPDDPSPYLL AIWTPGETAN SAQPPEQKCG GKASGKMCFD ETCSECNSLR EANSQTVRGT 1630 1640 1650 1660 1670 1680 LLIPCRTAMR GSFPLNGTYF QVNELFADHE SSLKPIDVPR DWIWDLPRRT VYFGTSVTSI 1690 1700 1710 1720 FRGLSTEQIQ FCFWKGFVCV RGFEQKTRAP RPLMARLHFP ASKLKNNKT
	Isoform 2 [UniParc]. Checksum: 74F337BA8947ACB6	FASTA	674	75,340
	10 20 30 40 50 60 MNSRADPGDR YFRVPLENQT QQEFMGSWIP FTPKKPRSSL MVDERVINQD LNGFPGGEFV 70 80 90 100 110 120 DRGFCNTGVD HNGVFDHGAH QGVTNLSMMI NSLAGSHAQA WSNSERDLLG RSEVTSPLAP 130 140 150 160 170 180 VIRNTTGNVE PVNGNFTSDV GMVNGPFTQS GTSQAGYNEF ELDDLLNPDQ MPFSFTSLLS 190 200 210 220 230 240 GGDSLFKVRQ YGPPACNKPL YNLNSPIRRE AVGSVCESSF QYVPSTPSLF RTGEKTGFLE 250 260 270 280 290 300 QIVTTTGHEI PEPKSDKSMG WVPLQPLPES LQLHLLELYP VLESIQKFLW PRLCKLDQRT 310 320 330 340 350 360 LYELHYQLIT FGKVFCTKSR PNCNACPMRG ECRHFASAYA SARLALPAPE ERSLTSATIP 370 380 390 400 410 420 VPPESYPPVA IPMIELPLPL EKSLASGAPS NRENCEPIIE EPASPGQECT EITESDIEDA 430 440 450 460 470 480 YYNEDPDEIP TIKLNIEQFG MTLREHMERN MELQEGDMSK ALVALHPTTT SIPTPKLKNI 490 500 510 520 530 540 SRLRTEHQVY ELPDSHRLLD GMDKREPDDP SPYLLAIWTP GETANSAQPP EQKCGGKASG 550 560 570 580 590 600 KMCFDETCSE CNSLREANSQ TVRGTLLIPC RTAMRGSFPL NGTYFQVNEL FADHESSLKP 610 620 630 640 650 660 IDVPRDWIWD LPRRTVYFGT SVTSIFRGLS TEQIQFCFWK GFVCVRGFEQ KTRAPRPLMA 670 RLHFPASKLK NNKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DEMETER, a DNA glycosylase domain protein, is required for endosperm gene imprinting and seed viability in Arabidopsis." Choi Y., Gehring M., Johnson L., Hannon M., Harada J.J., Goldberg R.B., Jacobsen S.E., Fischer R.L. Cell 110:33-42(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CHARACTERIZATION. Strain: cv. Columbia. Tissue: Flower.
[2]	"DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases." Morales-Ruiz T., Ortega-Galisteo A.P., Ponferrada-Marin M.I., Martinez-Macias M.I., Ariza R.R., Roldan-Arjona T. Proc. Natl. Acad. Sci. U.S.A. 103:6853-6858(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[7]	"An invariant aspartic acid in the DNA glycosylase domain of DEMETER is necessary for transcriptional activation of the imprinted MEDEA gene." Choi Y., Harada J.J., Goldberg R.B., Fischer R.L. Proc. Natl. Acad. Sci. U.S.A. 101:7481-7486(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-1562.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF521596 mRNA. Translation: AAM77215.1. DQ335243 mRNA. Translation: ABC61677.1. AL162875 Genomic DNA. Translation: CAB85562.1. Sequence problems. AL162875 Genomic DNA. Translation: CAB85563.1. Sequence problems. AL162875 Genomic DNA. Translation: CAB85564.1. Sequence problems. CP002688 Genomic DNA. Translation: AED90760.1. CP002688 Genomic DNA. Translation: AED90761.1. AK117994 mRNA. Translation: BAC42629.1. BT005357 mRNA. Translation: AAO63421.1.
IPI	IPI00516439. IPI00534354. IPI00760328.
PIR	T48452. T48453. T48454.
RefSeq	NP_001078527.1. NM_001085058.1. NP_196076.2. NM_120538.2.
UniGene	At.33104.
3D structure databases
ProteinModelPortal	Q8LK56.
ModBase	Search...
Proteomic databases
PaxDb	Q8LK56.
PRIDE	Q8LK56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G04560.2; AT5G04560.2; AT5G04560.
GeneID	830335.
KEGG	ath:AT5G04560.
Organism-specific databases
TAIR	At5g04560.
Phylogenomic databases
eggNOG	COG0177.
HOGENOM	HOG000112227.
InParanoid	Q8LK56.
OMA	CTEITES.
PhylomeDB	Q8LK56.
ProtClustDB	CLSN2690787.
Gene expression databases
Genevestigator	Q8LK56.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit.
InterPro	IPR011257. DNA_glycosylase. IPR003651. Endouclease3_FeS-loop_motif. IPR003265. HhH-GPD_domain. IPR023170. HTH_base_excis_C. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00525. FES. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
PROSITE	PS00764. ENDONUCLEASE_III_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DME_ARATH

Accession

Primary (citable) accession number: Q8LK56
Secondary accession number(s): Q1WEY5 , Q84TL4, Q9LZ67, Q9LZ68, Q9LZ69

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	June 13, 2006
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program