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Protein

tRNase Z TRZ1

Gene

TRZ1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Zinc phosphodiesterase, which displays tRNA 3'-processing endonuclease activity (PubMed:12032089, PubMed:16118225, PubMed:18052196, PubMed:19411372). Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA (PubMed:12032089, PubMed:18052196, PubMed:19411372). Can use bis-(p-nitophenyl) phosphate (bpNPP) as substrate (PubMed:18052196). Involved in the processing of small nucleolar RNAs (snoRNAs) (PubMed:19420328).5 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.1 Publication

Cofactori

Zn2+1 Publication, Ca2+1 Publication, Mn2+2 Publications, Mg2+2 PublicationsNote: Besides the tightly bound Zn2+ ion, TRZ1 requires additional Ca2+, Mn2+ or Mg2+ for pre-tRNA processing, while bpNPP hydrolysis occurs without addition of metal ions but is stimulated 2- to 3-fold when free Mn2+ or Zn2+ ions are added.1 Publication

Kineticsi

kcat is 7.4 sec(-1) with bpNPP as substrate.1 Publication
  1. KM=8.5 mM for bpNPP1 Publication

    GO - Molecular functioni

    • 3'-tRNA processing endoribonuclease activity Source: TAIR
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • tRNA 3'-end processing Source: TAIR
    • tRNA processing Source: TAIR

    Keywordsi

    Molecular functionEndonuclease, Hydrolase, Nuclease
    Biological processtRNA processing
    LigandCalcium, Magnesium, Manganese, Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.1.26.11. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNase Z TRZ11 Publication (EC:3.1.26.111 Publication)
    Alternative name(s):
    Nuclear ribonuclease Z1 Publication
    Short name:
    Nuclear RNase Z1 Publication
    Short tRNase Z 11 Publication
    Zinc phosphodiesterase NUZ1 Publication
    tRNA 3 endonuclease
    tRNase ZS11 Publication
    Short name:
    AthTRZS11 Publication
    Gene namesi
    Name:TRZ11 Publication
    Synonyms:NUZ1 Publication
    Ordered Locus Names:At1g74700Imported
    ORF Names:F1M20.38Imported, F25A4.32Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G74700.
    TAIRilocus:2019235. AT1G74700.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB-SubCell
    • nucleus Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype when grown under standard conditions.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi25C → G: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi40C → G: No effect. 1 Publication1
    Mutagenesisi51 – 60Missing : Loss of tRNA binding, but no effect on dimerization. 1 Publication10
    Mutagenesisi51F → L: No effect. 1 Publication1
    Mutagenesisi54H → L: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi56H → L: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi58D → A: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi59H → L: Loss of dimerization and catalytic activity. 1 Publication1
    Mutagenesisi62G → V: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi64P → A: No effect. 1 Publication1
    Mutagenesisi83P → L: Loss of dimerization and catalytic activity. 1 Publication1
    Mutagenesisi133H → L: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi140Y → L: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi178P → A: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi184G → V: Loss of tRNA binding, but no effect on dimerization. 1 Publication1
    Mutagenesisi185D → G: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi186T → I: Loss of dimerization and catalytic activity. 1 Publication1
    Mutagenesisi203K → I: Loss of dimerization and catalytic activity. 1 Publication1
    Mutagenesisi205L → I: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi208E → A: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi210T → I: No effect. 1 Publication1
    Mutagenesisi226H → L: Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi248H → L: Loss of dimerization and catalytic activity. 1 Publication1
    Mutagenesisi252R → G: Reduced pre-tRNA processing, but increased hydrolysis of bpNPP. No effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi252Missing : Loss of catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi253Y → S: Reduced catalytic activity, but no effect on dimerization and tRNA binding. 1 Publication1
    Mutagenesisi270 – 280Missing : Loss of dimerization and catalytic activity. 1 PublicationAdd BLAST11

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001558341 – 280tRNase Z TRZ1Add BLAST280

    Proteomic databases

    PaxDbiQ8LGU7.

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ8LGU7. baseline and differential.
    GenevisibleiQ8LGU7. AT.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT1G74700.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LGU7.
    SMRiQ8LGU7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminus (270-280) is essential for tRNA binding and processing activity.1 Publication

    Sequence similaritiesi

    Belongs to the RNase Z family.Curated

    Phylogenomic databases

    eggNOGiENOG410IIDX. Eukaryota.
    ENOG410ZMQJ. LUCA.
    HOGENOMiHOG000238909.
    InParanoidiQ8LGU7.
    KOiK00784.
    OMAiSRAAWHT.
    OrthoDBiEOG09360H2E.
    PhylomeDBiQ8LGU7.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiView protein in InterPro
    IPR001279. Metallo-B-lactamas.
    PfamiView protein in Pfam
    PF12706. Lactamase_B_2. 1 hit.
    SUPFAMiSSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8LGU7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKKKAMQIE GYPIEGLSIG GHETCIIFPS LRIAFDIGRC PHRAISQDFL
    60 70 80 90 100
    FISHSHMDHI GGLPMYVATR GLYKMKPPTI IVPASIKETV ESLFEVHRKL
    110 120 130 140 150
    DSSELKHNLV GLDIGEEFII RKDLKVKAFK TFHVIQSQGY VVYSTKYKLK
    160 170 180 190 200
    KEYIGLSGNE IKNLKVSGVE ITDSIITPEV AFTGDTTSDF VVDETNADAL
    210 220 230 240 250
    KAKVLVMEST FLDDSVSVEH ARDYGHIHIS EIVNHAEKFE NKAILLIHFS
    260 270 280
    ARYTVKEIED AVSALPPPLE GRVFALTQGF
    Length:280
    Mass (Da):31,333
    Last modified:February 21, 2006 - v3
    Checksum:i10126C052C8BB94D
    GO

    Sequence cautioni

    The sequence AAD55271 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence AAG52354 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ428989 mRNA. Translation: CAD22100.1.
    AC008263 Genomic DNA. Translation: AAD55271.1. Sequence problems.
    AC011765 Genomic DNA. Translation: AAG52354.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35623.1.
    PIRiC96776.
    RefSeqiNP_177608.2. NM_106128.3.
    UniGeneiAt.43240.

    Genome annotation databases

    EnsemblPlantsiAT1G74700.1; AT1G74700.1; AT1G74700.
    GeneIDi843809.
    GrameneiAT1G74700.1; AT1G74700.1; AT1G74700.
    KEGGiath:AT1G74700.

    Similar proteinsi

    Entry informationi

    Entry nameiRNZ1_ARATH
    AccessioniPrimary (citable) accession number: Q8LGU7
    Secondary accession number(s): Q9CA48, Q9SSF6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: February 21, 2006
    Last modified: June 7, 2017
    This is version 97 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families