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Q8LGU1 (AB8C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ABC transporter C family member 8
Short name=ABC transporter ABCC.8
Short name=AtABCC8
EC=3.6.3.44
Alternative name(s):
ATP-energized glutathione S-conjugate pump 6
Glutathione S-conjugate-transporting ATPase 6
Multidrug resistance-associated protein 6
Gene names
Name:ABCC8
Synonyms:MRP6, MRP8
Ordered Locus Names:At3g21250
ORF Names:MXL8.11
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pump for glutathione S-conjugates By similarity.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Ubiquitous. Ref.3

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence caution

The sequence BAB01717.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAD44995.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plant-type vacuole

Inferred from direct assay. Source: TAIR

vacuolar membrane

Inferred from direct assay. Source: TAIR

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8LGU1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Constructed according to the conserved gene model. No experimental confirmation available.
Isoform 2 (identifier: Q8LGU1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     782-792: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 14641442ABC transporter C family member 8
PRO_0000226077

Regions

Transmembrane27 – 4721Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane94 – 11421Helical; Potential
Transmembrane123 – 14321Helical; Potential
Transmembrane152 – 17221Helical; Potential
Transmembrane203 – 22321Helical; Potential
Transmembrane283 – 30321Helical; Potential
Transmembrane321 – 34323Helical; Potential
Transmembrane391 – 41121Helical; Potential
Transmembrane413 – 43321Helical; Potential
Transmembrane508 – 52821Helical; Potential
Transmembrane909 – 92921Helical; Potential
Transmembrane931 – 95121Helical; Potential
Transmembrane1008 – 102821Helical; Potential
Transmembrane1030 – 105021Helical; Potential
Transmembrane1132 – 115221Helical; Potential
Domain282 – 564283ABC transmembrane type-1 1
Domain600 – 821222ABC transporter 1
Domain901 – 1178278ABC transmembrane type-1 2
Domain1215 – 1449235ABC transporter 2
Nucleotide binding633 – 6408ATP 1 Potential
Nucleotide binding1249 – 12568ATP 2 Potential

Natural variations

Alternative sequence782 – 79211Missing in isoform 2.
VSP_041303

Experimental info

Sequence conflict3501M → L in CAD44995. Ref.3
Sequence conflict13011L → F in CAD44995. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 31, 2011. Version 3.
Checksum: E446F0F714FB4BDB

FASTA1,464162,072
        10         20         30         40         50         60 
MAAFIGSLPW CDVELNLASS CFQRTAIAFV NLLFLCIFYL FLIASCVSTH FIVRGRKKGW 

        70         80         90        100        110        120 
IFVAVAICCA ITSFIFLGVG LNSLIHGGND VTEISWVACF VEGIIWVSLA VSLLVNGSKW 

       130        140        150        160        170        180 
VNILVSVWWV SFALLDLVAK SGILLQGNGI RILDILTLPM SLLLLLCSWM NLRSSSAAAQ 

       190        200        210        220        230        240 
DCSVTGLSDP LLTKNPRKES ARLATAGFFS ILSFSWMNPL LSLGFKKPLS PEDIPSVVPE 

       250        260        270        280        290        300 
DEAQLAYKKF SQAWDTLLGD ESSTKERNLV FRAVVKVYFK ENIFIAVFAF LRTFAVVSLP 

       310        320        330        340        350        360 
LMLYVFVDYA NSDHRDLRNG FFNLACLVML KLVESLTMRH WYFASRRSGM RIRSALMVAA 

       370        380        390        400        410        420 
YKKQLKLSSL GRKRHSSGEI VNYIAVDAYR MGEFLWWFHS GWSLSLQLLL STAVLFGVVG 

       430        440        450        460        470        480 
AGAFPGLILL LLCGLLNLPF AKMLQNCQTQ FMIAQDKRLR STSEILNSMK VIKLQSWEDE 

       490        500        510        520        530        540 
FKKKIESCRD DEFTWLAKAQ LTKAFGSFLY WMSPTIVSSV VFLGCALLKS APLNASTIFT 

       550        560        570        580        590        600 
VLATLRVMSE PVKIIPDAIS AIIQGNVSFQ RLNNFLLDDE LKMDEIERSG LDASGTAVDI 

       610        620        630        640        650        660 
QVGNFGWEPE TKIPTLRNIH LEIKHGQKVA VCGPVGAGKS SLLHAVLGEI PKVSGTVKVF 

       670        680        690        700        710        720 
GSIAYVSQTS WIQSGTIRDN ILYGKPMESR RYNAAIKACA LDKDMNGFGH GDLTEIGQRG 

       730        740        750        760        770        780 
INLSGGQKQR IQLARAVYAD ADVYLLDDPF SAVDAHTAGV LFHKCVEDSL KEKTVILVTH 

       790        800        810        820        830        840 
QVEFLSEVDQ ILVMEEGTIT QSGKYEELLM MGTAFQQLVN AHNDAVTVLP LASNESLGDL 

       850        860        870        880        890        900 
RKEGKDREIR NMTVVEKIEE EIEKTDIPGV QLTQEEEKES GYVGMKPFLD YIGVSRGWCL 

       910        920        930        940        950        960 
LWSSVLGQVG FVVFQAASTY WLAFAIGIPK ITNTMLIGVY SIISTLSAGF VYARAITTAH 

       970        980        990       1000       1010       1020 
LGLKASKAFF SGFTNAVFKA PMLFFDSTPV GRILTRASSD LNVLDYDVPF AFIFVVAPAV 

      1030       1040       1050       1060       1070       1080 
ELTAALLIMT YVTWQVIIIA LLALAATKVV QDYYLASARE LIRINGTTKA PVMNYAAETS 

      1090       1100       1110       1120       1130       1140 
LGVVTIRAFG TAERFFKNYL NLVDADAVLF FLSNAAMEWV ILRIETLQNV TLFTCALLLI 

      1150       1160       1170       1180       1190       1200 
LIPKGYIAPG LVGLSLSYAL TLTQTQVFLT RWYCTLSNSI ISVERIKQYM NIPEEPPAII 

      1210       1220       1230       1240       1250       1260 
DDKRPPSSWP SNGTIHLQEL KIRYRPNAPL VLKGISCTFR EGTRVGVVGR TGSGKSTLIS 

      1270       1280       1290       1300       1310       1320 
ALFRLVEPAS GCILIDGIDI SKIGLKDLRM KLSIIPQEPT LFRGCIRTNL DPLGVYSDDE 

      1330       1340       1350       1360       1370       1380 
IWKALEKCQL KTTISNLPNK LDSSVSDEGE NWSVGQRQLF CLGRVLLKRN KILVLDEATA 

      1390       1400       1410       1420       1430       1440 
SIDSATDAII QRIIREEFAD CTVITVAHRV PTVIDSDMVM VLSFGDLVEY NEPSKLMETD 

      1450       1460 
SYFSKLVAEY WASCRGNSSQ NLQL

« Hide

Isoform 2 [UniParc].

Checksum: EEEF1D316D0353A4
Show »

FASTA1,453160,799

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana."
Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B.
Planta 216:107-119(2002) [PubMed: 12430019] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 79-1453 (ISOFORM 2), TISSUE SPECIFICITY.
[4]"The Arabidopsis thaliana ABC protein superfamily, a complete inventory."
Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.
J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Multifunctionality of plant ABC transporters -- more than just detoxifiers."
Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B.
Planta 214:345-355(2002) [PubMed: 11855639] [Abstract]
Cited for: GENE FAMILY.
[6]"Plant ABC proteins - a unified nomenclature and updated inventory."
Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.
Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB023045 Genomic DNA. Translation: BAB01717.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76481.1.
CP002686 Genomic DNA. Translation: AEE76482.1.
AJ507057 mRNA. Translation: CAD44995.1. Different initiation.
IPIIPI00540913.
IPI00991516.
RefSeqNP_001189944.1. NM_001203015.1.
NP_188762.3. NM_113020.3.
UniGeneAt.38092.

3D structure databases

ProteinModelPortalQ8LGU1.
SMRQ8LGU1. Positions 566-823, 884-1450.
ModBaseSearch...

Proteomic databases

PRIDEQ8LGU1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G21250.2; AT3G21250.2; AT3G21250.
GeneID821679.
GenomeReviewsGene locus AT3G21250 in contig BA000014_GR.
KEGGath:AT3G21250.

Organism-specific databases

TAIRAt3g21250.

Phylogenomic databases

eggNOGKOG0054.
GeneTreeEPGT00070000028299.
HOGENOMHBG758042.
InParanoidQ8LGU1.

Gene expression databases

GenevestigatorQ8LGU1.
GermOnlineAT3G21250. Arabidopsis thaliana.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAB8C_ARATH
AccessionPrimary (citable) accession number: Q8LGU1
Secondary accession number(s): Q9LU34
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 31, 2011
Last modified: December 14, 2011
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents