ID   SCPDH_ARATH             Reviewed;         454 AA.
AC   Q8LGI2; Q9ZR98;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 2.
DT   16-JUN-2009, entry version 38.
DE   RecName: Full=Probable mitochondrial saccharopine dehydrogenase At5g39410;
DE            Short=SDH;
DE            EC=1.5.1.9;
GN   OrderedLocusNames=At5g39410; ORFNames=MUL8.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RA   Lessard P., Petit-Jean X., Kreis M., Thomas M.;
RL   Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=98290546; PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV.
RT   Sequence features of the regions of 1,456,315 bp covered by nineteen
RT   physically assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=15276431; DOI=10.1016/j.phytochem.2004.03.028;
RA   Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S.,
RA   Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J.,
RA   Rolland N.;
RT   "The hydrophobic proteome of mitochondrial membranes from Arabidopsis
RT   cell suspensions.";
RL   Phytochemistry 65:1693-1707(2004).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14671022; DOI=10.1105/tpc.016055;
RA   Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA   Millar A.H.;
RT   "Experimental analysis of the Arabidopsis mitochondrial proteome
RT   highlights signaling and regulatory components, provides assessment of
RT   targeting prediction programs, and indicates plant-specific
RT   mitochondrial proteins.";
RL   Plant Cell 16:241-256(2004).
CC   -!- CATALYTIC ACTIVITY: N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD(+)
CC       + H(2)O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH.
CC   -!- PATHWAY: Amino-acid degradation; L-lysine degradation via
CC       saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane.
CC   -!- SIMILARITY: Belongs to the saccharopine dehydrogenase family.
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DR   EMBL; U83179; AAD09232.1; -; mRNA.
DR   EMBL; AB009054; BAB11014.1; -; Genomic_DNA.
DR   EMBL; AY084257; AAM60851.1; -; mRNA.
DR   IPI; IPI00529205; -.
DR   RefSeq; NP_568564.1; -.
DR   UniGene; At.49110; -.
DR   PRIDE; Q8LGI2; -.
DR   GeneID; 833937; -.
DR   GenomeReviews; BA000015_GR; AT5G39410.
DR   KEGG; ath:AT5G39410; -.
DR   NMPDR; fig|3702.1.peg.25637; -.
DR   TAIR; At5g39410; -.
DR   OMA; Q8LGI2; GTSCIDI.
DR   BRENDA; 1.5.1.9; 302.
DR   GermOnline; AT5G39410; Arabidopsis thaliana.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0005773; C:vacuole; IDA:TAIR.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0047131; F:saccharopine dehydrogenase (NAD+, L-glutama...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005097; Saccharopine_DH.
DR   Pfam; PF03435; Saccharop_dh; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Membrane; Mitochondrion; NAD; Oxidoreductase.
FT   CHAIN         1    454       Probable mitochondrial saccharopine
FT                                dehydrogenase At5g39410.
FT                                /FTId=PRO_0000226069.
FT   CONFLICT      8      8       E -> D (in Ref. 3; AAM60851).
FT   CONFLICT     13     13       M -> I (in Ref. 3; AAM60851).
FT   CONFLICT     38     38       Missing (in Ref. 3).
FT   CONFLICT    136    136       N -> K (in Ref. 3; AAM60851).
FT   CONFLICT    403    403       V -> L (in Ref. 3; AAM60851).
SQ   SEQUENCE   454 AA;  49681 MW;  7020142459682B6B CRC64;
     MNPTQKPEPV YDMVILGASG FTGKYVVREA LKFLQTPSSS PLKSLALAGR NPTRLTQSLE
     WAARPNPPPS SVAILTADTS DPDSLRRLCT QTKLILNCVG PFRIHGDPVV SACADSGCDY
     LDISGEPEFM ERMEANYHDR AEETGSLIVS ACGFDSIPAE LGLLFNAKQW VSPSVPNQIE
     AYLSLESDKK IAGNFGTYES AVLGVANAEK LKELRRSRPR RPRPTICGPP AKGPTLENQK
     TIGLWALKLP SADAVVVRRT LTTLTEKPHG LPGINESPEQ IQKREAFWSS IKPAHFGVKI
     TSKSLFGIFR YVTLGVSLGL LSKFSFGRWL LLKFPSVFSL GWFQKKGPSE EEVESATFKM
     WFIGRGYSEE SLASQGETKP DLEIITRISG PEIGYITTPI TLVQCGLIVL GQRESLVKGG
     VYTPGIVFGS TDIQQRLEDN GISFELISKI KTQG
//