Reviewed, UniProtKB/Swiss-Prot Q8LGI2 (SCPDH_ARATH)

Names and origin

Protein names

Recommended name:
    Probable mitochondrial saccharopine dehydrogenase At5g39410
      Short name=SDH
    EC=1.5.1.9

Gene names

Ordered Locus Names:	At5g39410
ORF Names:	MUL8.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH.
Pathway	Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.
Subcellular location	Mitochondrion membrane Ref.4 Ref.5.
Sequence similarities	Belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	binding Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Probable mitochondrial saccharopine dehydrogenase At5g39410		PRO_0000226069
Experimental info
	Sequence conflict	8	1	E → D in AAM60851. Ref.3
	Sequence conflict	13	1	M → I in AAM60851. Ref.3
	Sequence conflict	38	1	Missing Ref.3
	Sequence conflict	136	1	N → K in AAM60851. Ref.3
	Sequence conflict	403	1	V → L in AAM60851. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8LGI2-1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 7020142459682B6B	FASTA	454	49,681
	10 20 30 40 50 60 MNPTQKPEPV YDMVILGASG FTGKYVVREA LKFLQTPSSS PLKSLALAGR NPTRLTQSLE 70 80 90 100 110 120 WAARPNPPPS SVAILTADTS DPDSLRRLCT QTKLILNCVG PFRIHGDPVV SACADSGCDY 130 140 150 160 170 180 LDISGEPEFM ERMEANYHDR AEETGSLIVS ACGFDSIPAE LGLLFNAKQW VSPSVPNQIE 190 200 210 220 230 240 AYLSLESDKK IAGNFGTYES AVLGVANAEK LKELRRSRPR RPRPTICGPP AKGPTLENQK 250 260 270 280 290 300 TIGLWALKLP SADAVVVRRT LTTLTEKPHG LPGINESPEQ IQKREAFWSS IKPAHFGVKI 310 320 330 340 350 360 TSKSLFGIFR YVTLGVSLGL LSKFSFGRWL LLKFPSVFSL GWFQKKGPSE EEVESATFKM 370 380 390 400 410 420 WFIGRGYSEE SLASQGETKP DLEIITRISG PEIGYITTPI TLVQCGLIVL GQRESLVKGG 430 440 450 VYTPGIVFGS TDIQQRLEDN GISFELISKI KTQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lessard P., Petit-Jean X., Kreis M., Thomas M. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions." Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N. Phytochemistry 65:1693-1707(2004) [PubMed: 15276431] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83179 mRNA. Translation: AAD09232.1. AB009054 Genomic DNA. Translation: BAB11014.1. AY084257 mRNA. Translation: AAM60851.1.
IPI	IPI00529205.
RefSeq	NP_568564.1.
UniGene	At.30348 At.49110 Rsa.14416
3D structure databases
SMR	Q8LGI2. Positions 13-445.
ModBase	Search...
Proteomic databases
PRIDE	Q8LGI2.
Genome annotation databases
GeneID	833937.
GenomeReviews	Gene locus AT5G39410 in contig BA000015_GR.
KEGG	ath:AT5G39410.
NMPDR	fig|3702.1.peg.25637.
Organism-specific databases
TAIR	At5g39410.
Phylogenomic databases
HOGENOM	HBG527018.
InParanoid	Q8LGI2.
OMA	GTSCIDI.
PhylomeDB	Q8LGI2.
Enzyme and pathway databases
BRENDA	1.5.1.9. 302.
Gene expression databases
Genevestigator	Q8LGI2.
GermOnline	AT5G39410. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR005097. Saccharopine_DH/HSpermid_syn. [Graphical view]
Pfam	PF03435. Saccharop_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCPDH_ARATH

Accession

Primary (citable) accession number: Q8LGI2
Secondary accession number(s): Q9ZR98

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)