Reviewed, UniProtKB/Swiss-Prot Q8LGG0 (FKB12_ARATH)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl isomerase FKBP12
    EC=5.2.1.8
Alternative name(s):
    12 kDa FK506-binding protein
      Short name=12 kDa FKBP
    FKBP-12
    Immunophilin FKBP12
      Short name=AtFKBP12
    Peptidyl-prolyl cis-trans isomerase
      Short name=PPIase
      Short name=Rotamase

Gene names

Name:	FKBP12
Ordered Locus Names:	At5g64350
ORF Names:	MSJ1.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides By similarity.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Subunit structure	Interacts with FIP37 and with the immunosuppressive drug FK506. Its interaction with FIP37 is inhibited by FK506. Ref.5
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FKBP-type PPIase family. FKBP1 subfamily. Contains 1 PPIase FKBP-type domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Isomerase Rotamase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FIP37	Q9ZSZ8	3	EBI-1641228,EBI-1641243

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Peptidyl-prolyl isomerase FKBP12		PRO_0000075300
Regions
	Domain	19 – 112	94	PPIase FKBP-type
Amino acid modifications
	Disulfide bond	26 ↔ 80		By similarity
Experimental info
	Sequence conflict	111	1	V → L in AAM60880. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LGG0-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: C0ED5DFEB6C8E1E2	FASTA	112	11,988
	10 20 30 40 50 60 MGVEKQVIRP GNGPKPAPGQ TVTVHCTGFG KDGDLSQKFW STKDEGQKPF SFQIGKGAVI 70 80 90 100 110 KGWDEGVIGM QIGEVARLRC SSDYAYGAGG FPAWGIQPNS VLDFEIEVLS VQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a plant FKBP12 that does not mediate action of FK506 and rapamycin." Xu Q., Liang S., Kudla J., Luan S. Plant J. 15:511-519(1998) [PubMed: 9753776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506." Faure J.-D., Gingerich D., Howell S.H. Plant J. 15:783-789(1998) [PubMed: 9807817] [Abstract] Cited for: INTERACTION WITH FIP37 AND FK506. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U96924 mRNA. Translation: AAB57847.1. AB008268 Genomic DNA. Translation: BAB09866.1. AY059928 mRNA. Translation: AAL24410.1. AY081564 mRNA. Translation: AAM10126.1. AY084289 mRNA. Translation: AAM60880.1.
IPI	IPI00525181.
RefSeq	NP_201240.1.
UniGene	At.24046
3D structure databases
HSSP	HSSP built from PDB template 1KT1 based on UniProtKB Q9XSH5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LGG0. 1 interaction.
STRING	Q8LGG0.
Proteomic databases
PRIDE	Q8LGG0.
Genome annotation databases
GeneID	836556.
GenomeReviews	Gene locus AT5G64350 in contig BA000015_GR.
KEGG	ath:AT5G64350.
NMPDR	fig|3702.1.peg.28495.
Organism-specific databases
GeneFarm	5056.
TAIR	At5g64350.
Phylogenomic databases
OMA	GYLYDES.
Enzyme and pathway databases
BRENDA	5.2.1.8. 302.
Gene expression databases
Genevestigator	Q8LGG0.
GermOnline	AT5G64350. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001179. PPIase_FKBP. [Graphical view]
PANTHER	PTHR10516. PPIase_FKBP. 1 hit.
Pfam	PF00254. FKBP_C. 1 hit. [Graphical view]
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FKB12_ARATH

Accession

Primary (citable) accession number: Q8LGG0
Secondary accession number(s): O04263

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	November 3, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)