Q8LGF7 (PEX4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein PEROXIN-4
Short name=AtPEX4

Alternative name(s):
Probable ubiquitin-conjugating enzyme E2 21
EC=6.3.2.19
Ubiquitin carrier protein 21

Gene names

Name:	PEX4
Synonyms:	UBC21
Ordered Locus Names:	At5g25760
ORF Names:	F18A17.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity. Ref.7 Ref.8
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with PEX22. Ref.6
Subcellular location	Peroxisome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Peroxisome biogenesis Protein transport Transport Ubl conjugation pathway
Cellular component	Membrane Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from mutant phenotype Ref.7. Source: TAIR protein import into peroxisome matrix Inferred from mutant phenotype Ref.7. Source: TAIR
Cellular_component	peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Protein PEROXIN-4		PRO_0000345187
Sites
	Active site	90	1	Glycyl thioester intermediate By similarity
Experimental info
	Mutagenesis	123	1	P → L in pex4-1; reduced peroxisomal function and loss of response to indole-3-butyric acid. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q8LGF7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 36FB1E11684F48B4	FASTA	157	17,708
	10 20 30 40 50 60 MQASRARLFK EYKEVQREKV ADPDIQLICD DTNIFKWTAL IKGPSETPYE GGVFQLAFSV 70 80 90 100 110 120 PEPYPLQPPQ VRFLTKIFHP NVHFKTGEIC LDILKNAWSP AWTLQSVCRA IIALMAHPEP 130 140 150 DSPLNCDSGN LLRSGDVRGF NSMAQMYTRL AAMPKKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Identification and functional characterization of Arabidopsis PEROXIN4 and the interacting protein PEROXIN22." Zolman B.K., Monroe-Augustus M., Silva I.D., Bartel B. Plant Cell 17:3422-3435(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, MUTAGENESIS OF PRO-123, INTERACTION WITH PEX22.
[7]	"Functional classification of Arabidopsis peroxisome biogenesis factors proposed from analyses of knockdown mutants." Nito K., Kamigaki A., Kondo M., Hayashi M., Nishimura M. Plant Cell Physiol. 48:763-774(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Peroxisome-associated matrix protein degradation in Arabidopsis." Lingard M.J., Monroe-Augustus M., Bartel B. Proc. Natl. Acad. Sci. U.S.A. 106:4561-4566(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ027035 mRNA. Translation: AAY44861.1. AC005405 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED93482.1. CP002688 Genomic DNA. Translation: AED93483.1. AY084297 mRNA. Translation: AAM60888.1. BT025616 mRNA. Translation: ABF59034.1.
IPI	IPI00537759.
RefSeq	NP_001031939.1. NM_001036862.1. NP_568476.1. NM_122477.2.
UniGene	At.20548. At.30893.
3D structure databases
HSSP	HSSP built from PDB template 2C2V based on UniProtKB P61088.
ProteinModelPortal	Q8LGF7.
SMR	Q8LGF7. Positions 7-125.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LGF7. 1 interaction.
STRING	3702.AT5G25760.1-P.
Proteomic databases
PaxDb	Q8LGF7.
PRIDE	Q8LGF7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G25760.1; AT5G25760.1; AT5G25760. AT5G25760.2; AT5G25760.2; AT5G25760.
GeneID	832645.
KEGG	ath:AT5G25760.
Organism-specific databases
TAIR	At5g25760.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233455.
InParanoid	Q8LGF7.
KO	K10689.
OMA	NCDAGNL.
ProtClustDB	CLSN2689869.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q8LGF7.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEX4_ARATH

Accession

Primary (citable) accession number: Q8LGF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program