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Protein

Protein PEROXIN-4

Gene

PEX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation Source: TAIR
  • peroxisome organization Source: TAIR
  • protein import into peroxisome matrix Source: TAIR
  • protein ubiquitination Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processPeroxisome biogenesis, Protein transport, Transport, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PEROXIN-4
Short name:
AtPEX4
Alternative name(s):
E2 ubiquitin-conjugating enzyme 21
Probable ubiquitin-conjugating enzyme E2 21 (EC:2.3.2.23)
Ubiquitin carrier protein 21
Gene namesi
Name:PEX4
Synonyms:UBC21
Ordered Locus Names:At5g25760
ORF Names:F18A17.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G25760
TAIRilocus:2145269 AT5G25760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi123P → L in pex4-1; reduced peroxisomal function and loss of response to indole-3-butyric acid. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451871 – 157Protein PEROXIN-4Add BLAST157

Proteomic databases

PaxDbiQ8LGF7
PRIDEiQ8LGF7

Expressioni

Gene expression databases

GenevisibleiQ8LGF7 AT

Interactioni

Subunit structurei

Interacts with PEX22.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi179201 interactor.
IntActiQ8LGF7 1 interactor.
STRINGi3702.AT5G25760.1

Structurei

3D structure databases

ProteinModelPortaliQ8LGF7
SMRiQ8LGF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
KOiK10689
OMAiCVRAVRM
OrthoDBiEOG09360P8Q
PhylomeDBiQ8LGF7

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.101 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8LGF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQASRARLFK EYKEVQREKV ADPDIQLICD DTNIFKWTAL IKGPSETPYE
60 70 80 90 100
GGVFQLAFSV PEPYPLQPPQ VRFLTKIFHP NVHFKTGEIC LDILKNAWSP
110 120 130 140 150
AWTLQSVCRA IIALMAHPEP DSPLNCDSGN LLRSGDVRGF NSMAQMYTRL

AAMPKKG
Length:157
Mass (Da):17,708
Last modified:October 1, 2002 - v1
Checksum:i36FB1E11684F48B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027035 mRNA Translation: AAY44861.1
AC005405 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93482.1
CP002688 Genomic DNA Translation: AED93483.1
AY084297 mRNA Translation: AAM60888.1
BT025616 mRNA Translation: ABF59034.1
RefSeqiNP_001031939.1, NM_001036862.2
NP_568476.1, NM_122477.2
UniGeneiAt.20548
At.30893

Genome annotation databases

EnsemblPlantsiAT5G25760.1; AT5G25760.1; AT5G25760
AT5G25760.2; AT5G25760.2; AT5G25760
GeneIDi832645
GrameneiAT5G25760.1; AT5G25760.1; AT5G25760
AT5G25760.2; AT5G25760.2; AT5G25760
KEGGiath:AT5G25760

Similar proteinsi

Entry informationi

Entry nameiPEX4_ARATH
AccessioniPrimary (citable) accession number: Q8LGF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome