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Protein

Protein PEROXIN-4

Gene

PEX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • fatty acid beta-oxidation Source: TAIR
  • peroxisome organization Source: TAIR
  • protein import into peroxisome matrix Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Peroxisome biogenesis, Protein transport, Transport, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PEROXIN-4
Short name:
AtPEX4
Alternative name(s):
E2 ubiquitin-conjugating enzyme 21
Probable ubiquitin-conjugating enzyme E2 21 (EC:2.3.2.23)
Ubiquitin carrier protein 21
Gene namesi
Name:PEX4
Synonyms:UBC21
Ordered Locus Names:At5g25760
ORF Names:F18A17.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G25760.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi123P → L in pex4-1; reduced peroxisomal function and loss of response to indole-3-butyric acid. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451871 – 157Protein PEROXIN-4Add BLAST157

Proteomic databases

PaxDbiQ8LGF7.
PRIDEiQ8LGF7.

Expressioni

Gene expression databases

GenevisibleiQ8LGF7. AT.

Interactioni

Subunit structurei

Interacts with PEX22.1 Publication

Protein-protein interaction databases

BioGridi17920. 1 interactor.
IntActiQ8LGF7. 1 interactor.
STRINGi3702.AT5G25760.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LGF7.
SMRiQ8LGF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
HOGENOMiHOG000233455.
KOiK10689.
OMAiRMYTQLY.
OrthoDBiEOG09360P8Q.
PhylomeDBiQ8LGF7.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LGF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQASRARLFK EYKEVQREKV ADPDIQLICD DTNIFKWTAL IKGPSETPYE
60 70 80 90 100
GGVFQLAFSV PEPYPLQPPQ VRFLTKIFHP NVHFKTGEIC LDILKNAWSP
110 120 130 140 150
AWTLQSVCRA IIALMAHPEP DSPLNCDSGN LLRSGDVRGF NSMAQMYTRL

AAMPKKG
Length:157
Mass (Da):17,708
Last modified:October 1, 2002 - v1
Checksum:i36FB1E11684F48B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027035 mRNA. Translation: AAY44861.1.
AC005405 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93482.1.
CP002688 Genomic DNA. Translation: AED93483.1.
AY084297 mRNA. Translation: AAM60888.1.
BT025616 mRNA. Translation: ABF59034.1.
RefSeqiNP_001031939.1. NM_001036862.2.
NP_568476.1. NM_122477.2.
UniGeneiAt.20548.
At.30893.

Genome annotation databases

EnsemblPlantsiAT5G25760.1; AT5G25760.1; AT5G25760.
AT5G25760.2; AT5G25760.2; AT5G25760.
GeneIDi832645.
GrameneiAT5G25760.1; AT5G25760.1; AT5G25760.
AT5G25760.2; AT5G25760.2; AT5G25760.
KEGGiath:AT5G25760.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ027035 mRNA. Translation: AAY44861.1.
AC005405 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93482.1.
CP002688 Genomic DNA. Translation: AED93483.1.
AY084297 mRNA. Translation: AAM60888.1.
BT025616 mRNA. Translation: ABF59034.1.
RefSeqiNP_001031939.1. NM_001036862.2.
NP_568476.1. NM_122477.2.
UniGeneiAt.20548.
At.30893.

3D structure databases

ProteinModelPortaliQ8LGF7.
SMRiQ8LGF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17920. 1 interactor.
IntActiQ8LGF7. 1 interactor.
STRINGi3702.AT5G25760.1.

Proteomic databases

PaxDbiQ8LGF7.
PRIDEiQ8LGF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G25760.1; AT5G25760.1; AT5G25760.
AT5G25760.2; AT5G25760.2; AT5G25760.
GeneIDi832645.
GrameneiAT5G25760.1; AT5G25760.1; AT5G25760.
AT5G25760.2; AT5G25760.2; AT5G25760.
KEGGiath:AT5G25760.

Organism-specific databases

TAIRiAT5G25760.

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
HOGENOMiHOG000233455.
KOiK10689.
OMAiRMYTQLY.
OrthoDBiEOG09360P8Q.
PhylomeDBiQ8LGF7.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ8LGF7.

Gene expression databases

GenevisibleiQ8LGF7. AT.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEX4_ARATH
AccessioniPrimary (citable) accession number: Q8LGF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.