Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8LGF7

- PEX4_ARATH

UniProt

Q8LGF7 - PEX4_ARATH

Protein

Protein PEROXIN-4

Gene

PEX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Required for peroxisome biogenesis. Necessary for the developmental elimination of obsolete peroxisome matrix proteins. May be involved in the ubiquitination of PEX5, targeting it for recycling. Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: TAIR
    4. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. fatty acid beta-oxidation Source: TAIR
    2. peroxisome organization Source: TAIR
    3. protein import into peroxisome matrix Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Peroxisome biogenesis, Protein transport, Transport, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G25760-MONOMER.
    ARA:GQT-755-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein PEROXIN-4
    Short name:
    AtPEX4
    Alternative name(s):
    Probable ubiquitin-conjugating enzyme E2 21 (EC:6.3.2.19)
    Ubiquitin carrier protein 21
    Gene namesi
    Name:PEX4
    Synonyms:UBC21
    Ordered Locus Names:At5g25760
    ORF Names:F18A17.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G25760.

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi123 – 1231P → L in pex4-1; reduced peroxisomal function and loss of response to indole-3-butyric acid. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Protein PEROXIN-4PRO_0000345187Add
    BLAST

    Proteomic databases

    PaxDbiQ8LGF7.
    PRIDEiQ8LGF7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8LGF7.

    Interactioni

    Subunit structurei

    Interacts with PEX22.1 Publication

    Protein-protein interaction databases

    BioGridi17920. 1 interaction.
    IntActiQ8LGF7. 1 interaction.
    STRINGi3702.AT5G25760.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LGF7.
    SMRiQ8LGF7. Positions 7-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiQ8LGF7.
    KOiK10689.
    OMAiHPNIHFK.
    PhylomeDBiQ8LGF7.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8LGF7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQASRARLFK EYKEVQREKV ADPDIQLICD DTNIFKWTAL IKGPSETPYE    50
    GGVFQLAFSV PEPYPLQPPQ VRFLTKIFHP NVHFKTGEIC LDILKNAWSP 100
    AWTLQSVCRA IIALMAHPEP DSPLNCDSGN LLRSGDVRGF NSMAQMYTRL 150
    AAMPKKG 157
    Length:157
    Mass (Da):17,708
    Last modified:October 1, 2002 - v1
    Checksum:i36FB1E11684F48B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027035 mRNA. Translation: AAY44861.1.
    AC005405 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93482.1.
    CP002688 Genomic DNA. Translation: AED93483.1.
    AY084297 mRNA. Translation: AAM60888.1.
    BT025616 mRNA. Translation: ABF59034.1.
    RefSeqiNP_001031939.1. NM_001036862.1.
    NP_568476.1. NM_122477.2.
    UniGeneiAt.20548.
    At.30893.

    Genome annotation databases

    EnsemblPlantsiAT5G25760.1; AT5G25760.1; AT5G25760.
    AT5G25760.2; AT5G25760.2; AT5G25760.
    GeneIDi832645.
    KEGGiath:AT5G25760.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ027035 mRNA. Translation: AAY44861.1 .
    AC005405 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED93482.1 .
    CP002688 Genomic DNA. Translation: AED93483.1 .
    AY084297 mRNA. Translation: AAM60888.1 .
    BT025616 mRNA. Translation: ABF59034.1 .
    RefSeqi NP_001031939.1. NM_001036862.1.
    NP_568476.1. NM_122477.2.
    UniGenei At.20548.
    At.30893.

    3D structure databases

    ProteinModelPortali Q8LGF7.
    SMRi Q8LGF7. Positions 7-127.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17920. 1 interaction.
    IntActi Q8LGF7. 1 interaction.
    STRINGi 3702.AT5G25760.1-P.

    Proteomic databases

    PaxDbi Q8LGF7.
    PRIDEi Q8LGF7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G25760.1 ; AT5G25760.1 ; AT5G25760 .
    AT5G25760.2 ; AT5G25760.2 ; AT5G25760 .
    GeneIDi 832645.
    KEGGi ath:AT5G25760.

    Organism-specific databases

    TAIRi AT5G25760.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi Q8LGF7.
    KOi K10689.
    OMAi HPNIHFK.
    PhylomeDBi Q8LGF7.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G25760-MONOMER.
    ARA:GQT-755-MONOMER.

    Gene expression databases

    Genevestigatori Q8LGF7.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Identification and functional characterization of Arabidopsis PEROXIN4 and the interacting protein PEROXIN22."
      Zolman B.K., Monroe-Augustus M., Silva I.D., Bartel B.
      Plant Cell 17:3422-3435(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, MUTAGENESIS OF PRO-123, INTERACTION WITH PEX22.
    7. "Functional classification of Arabidopsis peroxisome biogenesis factors proposed from analyses of knockdown mutants."
      Nito K., Kamigaki A., Kondo M., Hayashi M., Nishimura M.
      Plant Cell Physiol. 48:763-774(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Peroxisome-associated matrix protein degradation in Arabidopsis."
      Lingard M.J., Monroe-Augustus M., Bartel B.
      Proc. Natl. Acad. Sci. U.S.A. 106:4561-4566(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPEX4_ARATH
    AccessioniPrimary (citable) accession number: Q8LGF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3