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Protein

Actin-related protein 6

Gene

ARP6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Binds to the promoter region of FLC chromatin. Required for the activation of FLC and FLC/MAF genes expression to levels that inhibit flowering, through both histone H3 and H4 acetylation and methylation mechanisms. Involved in several developmental processes including organization of plant organs, leaves formation, flowering time repression, and fertility. Modulates photoperiod-dependent epidermal leaves cell development; promotes cell division in long days, and cell expansion/division in short days. May be involved in the regulation of pathogenesis-related proteins (PRs).6 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

  • actin filament-based process Source: TAIR
  • cell proliferation Source: TAIR
  • chromatin remodeling Source: TAIR
  • defense response Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of flower development Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to temperature stimulus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 6
Alternative name(s):
Protein EARLY IN SHORT DAYS 1
Protein SUPPRESSOR OF FRIGIDA 3
Gene namesi
Name:ARP6
Synonyms:ESD1, SUF3
Ordered Locus Names:At3g33520
ORF Names:T4P3.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G33520.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Early flowering, leaf serration, production of extra petals and weak apical dominance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Actin-related protein 6PRO_0000320535Add
BLAST

Proteomic databases

PaxDbiQ8LGE3.
PRIDEiQ8LGE3.

Expressioni

Tissue specificityi

Mostly expressed in flowers, and, to a lower extent, in seedlings, shoot apex, stems, siliques, seeds, and roots (at protein level).4 Publications

Gene expression databases

GenevisibleiQ8LGE3. AT.

Interactioni

Subunit structurei

Component of the SWR1 chromatin-remodeling complex composed of at least ARP6/ESD1/SUF3, PIE1, SWC6, SWC2 and H2AZs (HTA8, HTA9, HTA11). Interacts directly with SWC6/SEF and PIE1. Also interacts with H2A.F/Z proteins.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PIE1Q7X9V24EBI-1537316,EBI-1537462
SWC6Q9FHW26EBI-1537316,EBI-1537353

Protein-protein interaction databases

BioGridi8476. 2 interactions.
IntActiQ8LGE3. 2 interactions.
STRINGi3702.AT3G33520.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LGE3.
SMRiQ8LGE3. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP6 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
InParanoidiQ8LGE3.
KOiK11662.
OMAiHLTSWET.
PhylomeDBiQ8LGE3.

Family and domain databases

InterProiIPR004000. Actin.
IPR030054. Arp6.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 2 hits.
PTHR11937:SF47. PTHR11937:SF47. 2 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LGE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIVVLDNG GGLIKAGQGG ERDPTTVIPN CLYKPLSSKK FIHPSPLTTL
60 70 80 90 100
SDEIDLTSAA VRRPIDRGYL INSDLQREIW SHLFTSLLHI APSSSSLLLT
110 120 130 140 150
EAPLSIPSVQ RTTDELVFED FGFSSLYIAH PQSLVHLYEA SRQPDSILSK
160 170 180 190 200
TQCSLVVDCG FSFTHAVPVL HNFTLNHAIK RIDLGGKAFT NYLKELVSYR
210 220 230 240 250
SINVMDETFL VDDAKEKLCF VSLDLLRDLR LARNGNTLIK STYVLPDGVT
260 270 280 290 300
HTKGYVKDPQ AAKRFLSLSE KESVVVMDKV GERKKADMNK NEIDLTNERF
310 320 330 340 350
LVPETLFQPA DLGMNQAGLA ECIVRAINSC HSYLQPVLYQ SIILTGGSTL
360 370 380 390 400
FPQLKERLEG ELRPLVPDHF DVKITTQEDP ILGVWRGGSL LASSPDFESM
410 420
CVTKAEYEEL GSARCRRRFF H
Length:421
Mass (Da):46,944
Last modified:October 1, 2002 - v1
Checksum:i9854EDAB8630030F
GO

Sequence cautioni

The sequence AAF03459.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → MG in AAM53246 (PubMed:11891255).Curated
Sequence conflicti6 – 61V → F in AAM53246 (PubMed:11891255).Curated
Sequence conflicti250 – 2501T → I in AAK92721 (PubMed:14593172).Curated
Sequence conflicti250 – 2501T → I in AAN77965 (PubMed:12566397).Curated
Sequence conflicti291 – 2911N → I in AAN77939 (PubMed:12566397).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti230 – 2312RL → PP in strain: cv. Lip-0.
Natural varianti231 – 2311L → P in strain: cv. Aa-0. 1 Publication
Natural varianti248 – 2481G → S in strain: cv. Goe-2. 1 Publication
Natural varianti250 – 2501T → R in strain: cv. Ri-0. 1 Publication
Natural varianti253 – 2531K → E in strain: cv. Gre-0 and cv. Kas-1. 1 Publication
Natural varianti263 – 2631K → R in strain: cv. Di-0 and cv. Kil-0. 1 Publication
Natural varianti271 – 2711K → E in strain: cv. Cl-0. 1 Publication
Natural varianti280 – 2801V → D in strain: cv. Yo-0. 1 Publication
Natural varianti290 – 2901K → E in strain: cv. Kil-0. 1 Publication
Natural varianti301 – 3011L → I in strain: cv. Yo-0. 1 Publication
Natural varianti308 – 3114QPAD → SLQI in strain: cv. Ba-1.
Natural varianti310 – 3101A → S in strain: cv. En-2. 1 Publication
Natural varianti311 – 3111D → V in strain: cv. Oy-0. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507914 mRNA. Translation: AAM53246.1.
AC009992 Genomic DNA. Translation: AAF03459.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77716.1.
AY050786 mRNA. Translation: AAK92721.1.
AY150426 mRNA. Translation: AAN12968.1.
AY084321 mRNA. Translation: AAM60907.1.
AF494760 Genomic DNA. Translation: AAN77933.1.
AF494761 Genomic DNA. Translation: AAN77934.1.
AF494765 Genomic DNA. Translation: AAN77938.1.
AF494766 Genomic DNA. Translation: AAN77939.1.
AF494767 Genomic DNA. Translation: AAN77940.1.
AF494770 Genomic DNA. Translation: AAN77943.1.
AF494772 Genomic DNA. Translation: AAN77945.1.
AF494773 Genomic DNA. Translation: AAN77946.1.
AF494774 Genomic DNA. Translation: AAN77947.1.
AF494776 Genomic DNA. Translation: AAN77949.1.
AF494777 Genomic DNA. Translation: AAN77950.1.
AF494782 Genomic DNA. Translation: AAN77955.1.
AF494789 Genomic DNA. Translation: AAN77962.1.
AF494792 Genomic DNA. Translation: AAN77965.1.
AF494796 Genomic DNA. Translation: AAN77969.1.
BK000425 Genomic DNA. Translation: DAA00030.1.
RefSeqiNP_566861.1. NM_114070.3.
UniGeneiAt.18479.
At.18481.

Genome annotation databases

EnsemblPlantsiAT3G33520.1; AT3G33520.1; AT3G33520.
GeneIDi823150.
GrameneiAT3G33520.1; AT3G33520.1; AT3G33520.
KEGGiath:AT3G33520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507914 mRNA. Translation: AAM53246.1.
AC009992 Genomic DNA. Translation: AAF03459.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77716.1.
AY050786 mRNA. Translation: AAK92721.1.
AY150426 mRNA. Translation: AAN12968.1.
AY084321 mRNA. Translation: AAM60907.1.
AF494760 Genomic DNA. Translation: AAN77933.1.
AF494761 Genomic DNA. Translation: AAN77934.1.
AF494765 Genomic DNA. Translation: AAN77938.1.
AF494766 Genomic DNA. Translation: AAN77939.1.
AF494767 Genomic DNA. Translation: AAN77940.1.
AF494770 Genomic DNA. Translation: AAN77943.1.
AF494772 Genomic DNA. Translation: AAN77945.1.
AF494773 Genomic DNA. Translation: AAN77946.1.
AF494774 Genomic DNA. Translation: AAN77947.1.
AF494776 Genomic DNA. Translation: AAN77949.1.
AF494777 Genomic DNA. Translation: AAN77950.1.
AF494782 Genomic DNA. Translation: AAN77955.1.
AF494789 Genomic DNA. Translation: AAN77962.1.
AF494792 Genomic DNA. Translation: AAN77965.1.
AF494796 Genomic DNA. Translation: AAN77969.1.
BK000425 Genomic DNA. Translation: DAA00030.1.
RefSeqiNP_566861.1. NM_114070.3.
UniGeneiAt.18479.
At.18481.

3D structure databases

ProteinModelPortaliQ8LGE3.
SMRiQ8LGE3. Positions 5-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi8476. 2 interactions.
IntActiQ8LGE3. 2 interactions.
STRINGi3702.AT3G33520.1.

Proteomic databases

PaxDbiQ8LGE3.
PRIDEiQ8LGE3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G33520.1; AT3G33520.1; AT3G33520.
GeneIDi823150.
GrameneiAT3G33520.1; AT3G33520.1; AT3G33520.
KEGGiath:AT3G33520.

Organism-specific databases

TAIRiAT3G33520.

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
InParanoidiQ8LGE3.
KOiK11662.
OMAiHLTSWET.
PhylomeDBiQ8LGE3.

Miscellaneous databases

PROiQ8LGE3.

Gene expression databases

GenevisibleiQ8LGE3. AT.

Family and domain databases

InterProiIPR004000. Actin.
IPR030054. Arp6.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 2 hits.
PTHR11937:SF47. PTHR11937:SF47. 2 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains ancient classes of differentially expressed actin-related protein genes."
    McKinney E.C., Kandasamy M.K., Meagher R.B.
    Plant Physiol. 128:997-1007(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, TISSUE SPECIFICITY, GENE FAMILY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Centromere satellites from Arabidopsis populations: maintenance of conserved and variable domains."
    Hall S.E., Kettler G., Preuss D.
    Genome Res. 13:195-205(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 230-312, VARIANTS 230-PRO-PRO-231; PRO-231; SER-248; ARG-250; GLU-253; ARG-263; GLU-271; ASP-280; GLU-290; ILE-301; 308-SER--ILE-311; SER-310 AND VAL-311.
    Strain: cv. Aa-0, cv. Ba-1, cv. Cl-0, cv. Columbia, cv. Di-0, cv. En-2, cv. Goe-2, cv. Gre-0, cv. Hi-0, cv. Kas-1, cv. Kil-0, cv. Lip-0, cv. Oy-0, cv. Ri-0 and cv. Yo-0.
  7. Cited for: REVIEW, GENE FAMILY, NOMENCLATURE.
  8. "The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repression of flowering in Arabidopsis."
    Deal R.B., Kandasamy M.K., McKinney E.C., Meagher R.B.
    Plant Cell 17:2633-2646(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  9. "SUPPRESSOR OF FRIGIDA3 encodes a nuclear ACTIN-RELATED PROTEIN6 required for floral repression in Arabidopsis."
    Choi K., Kim S., Kim S.Y., Kim M., Hyun Y., Lee H., Choe S., Kim S.-G., Michaels S., Lee I.
    Plant Cell 17:2647-2660(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  10. "EARLY IN SHORT DAYS 1 (ESD1) encodes ACTIN-RELATED PROTEIN 6 (AtARP6), a putative component of chromatin remodelling complexes that positively regulates FLC accumulation in Arabidopsis."
    Martin-Trillo M., Lazaro A., Poethig R.S., Gomez-Mena C., Pineiro M.A., Martinez-Zapater J.M., Jarillo J.A.
    Development 133:1241-1252(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "Arabidopsis homologs of components of the SWR1 complex regulate flowering and plant development."
    Choi K., Park C., Lee J., Oh M., Noh B., Lee I.
    Development 134:1931-1941(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SWC6 AND PIE1, SUBUNIT.
  12. "Repression of flowering in Arabidopsis requires activation of FLOWERING LOCUS C expression by the histone variant H2A.Z."
    Deal R.B., Topp C.N., McKinney E.C., Meagher R.B.
    Plant Cell 19:74-83(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, IDENTIFICATION IN THE SWR1 COMPLEX, INTERACTION WITH H2A.F/Z PROTEINS.
  13. "SEF, a new protein required for flowering repression in Arabidopsis, interacts with PIE1 and ARP6."
    March-Diaz R., Garcia-Dominguez M., Florencio F.J., Reyes J.C.
    Plant Physiol. 143:893-901(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIE1 AND SWC6.
  14. "Histone H2A.Z and homologues of components of the SWR1 complex are required to control immunity in Arabidopsis."
    March-Diaz R., Garcia-Dominguez M., Lozano-Juste J., Leon J., Florencio F.J., Reyes J.C.
    Plant J. 53:475-487(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiARP6_ARATH
AccessioniPrimary (citable) accession number: Q8LGE3
Secondary accession number(s): Q8GZL0
, Q8GZL1, Q8GZL2, Q8GZL3, Q8GZL4, Q8GZL5, Q8GZL6, Q8GZL7, Q8GZL8, Q8GZL9, Q8GZM0, Q8GZM1, Q8GZM2, Q8GZM3, Q8GZM4, Q8LKR0, Q949Z7, Q9SRK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2002
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.