Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin thioesterase otubain-like

Gene

At1g28120

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89By similarity1
Active sitei92NucleophileBy similarity1
Active sitei259By similarity1
Active sitei288By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689896 Ovarian tumor domain proteases

Protein family/group databases

MEROPSiC65.001

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase otubain-like (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like
Gene namesi
Ordered Locus Names:At1g28120
ORF Names:F13K9.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G28120
TAIRilocus:2010469 AT1G28120

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002210141 – 306Ubiquitin thioesterase otubain-likeAdd BLAST306

Proteomic databases

PaxDbiQ8LG98

Expressioni

Gene expression databases

ExpressionAtlasiQ8LG98 baseline and differential
GenevisibleiQ8LG98 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi24940, 4 interactors
IntActiQ8LG98, 2 interactors
STRINGi3702.AT1G28120.1

Structurei

3D structure databases

ProteinModelPortaliQ8LG98
SMRiQ8LG98
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 295OTUPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the peptidase C65 family.Curated

Phylogenomic databases

eggNOGiKOG3991 Eukaryota
ENOG410ZMN7 LUCA
HOGENOMiHOG000019496
InParanoidiQ8LG98
KOiK09602
OMAiEDFHETF
OrthoDBiEOG09360JHL
PhylomeDBiQ8LG98

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR016615 Otubain
IPR019400 Peptidase_C65_otubain
PANTHERiPTHR12931 PTHR12931, 1 hit
PfamiView protein in Pfam
PF10275 Peptidase_C65, 1 hit
PIRSFiPIRSF013503 Ubiquitin_thioesterase_Otubain, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit

Sequencei

Sequence statusi: Complete.

Q8LG98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNQIDMVKD EAEVAASISA IKGEEWGNCS SVEDQPSFQE EEAAKVPYVG
60 70 80 90 100
DKEPLSSLAA EYQSGSPILL EKIKILDSQY IGIRRTRGDG NCFFRSFMFS
110 120 130 140 150
YLEHILESQD RAEVDRIKVN VEKCRKTLQN LGYTDFTFED FFALFLEQLD
160 170 180 190 200
DILQGTEESI SYDELVNRSR DQSVSDYIVM FFRFVTAGDI RTRADFFEPF
210 220 230 240 250
ITGLSNATVD QFCKSSVEPM GEESDHIHIT ALSDALGVAI RVVYLDRSSC
260 270 280 290 300
DSGGVTVNHH DFVPVGITNE KDEEASAPFI TLLYRPGHYD ILYPKPSCKV

SDNVGK
Length:306
Mass (Da):34,434
Last modified:March 1, 2004 - v2
Checksum:i7D8822E69806AC87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5I → N in AAM60966 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069471 Genomic DNA Translation: AAG51478.1
CP002684 Genomic DNA Translation: AEE30920.1
AY136373 mRNA Translation: AAM97039.1
BT000164 mRNA Translation: AAN15483.1
AY084389 mRNA Translation: AAM60966.1
PIRiA86407
RefSeqiNP_564299.1, NM_102577.4
UniGeneiAt.43774

Genome annotation databases

EnsemblPlantsiAT1G28120.1; AT1G28120.1; AT1G28120
GeneIDi839705
GrameneiAT1G28120.1; AT1G28120.1; AT1G28120
KEGGiath:AT1G28120

Similar proteinsi

Entry informationi

Entry nameiOTUBL_ARATH
AccessioniPrimary (citable) accession number: Q8LG98
Secondary accession number(s): Q9C7E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: April 25, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health