Q8LG98 (OTUBL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase otubain-like
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like

Gene names

Ordered Locus Names:	At1g28120
ORF Names:	F13K9.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C65 family. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW omega peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Ubiquitin thioesterase otubain-like		PRO_0000221014
Regions
	Domain	81 – 295	215	OTU
Sites
	Active site	89	1	By similarity
	Active site	92	1	Nucleophile By similarity
	Active site	288	1	By similarity
Experimental info
	Sequence conflict	5	1	I → N in AAM60966. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LG98 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 7D8822E69806AC87	FASTA	306	34,434
	10 20 30 40 50 60 MQNQIDMVKD EAEVAASISA IKGEEWGNCS SVEDQPSFQE EEAAKVPYVG DKEPLSSLAA 70 80 90 100 110 120 EYQSGSPILL EKIKILDSQY IGIRRTRGDG NCFFRSFMFS YLEHILESQD RAEVDRIKVN 130 140 150 160 170 180 VEKCRKTLQN LGYTDFTFED FFALFLEQLD DILQGTEESI SYDELVNRSR DQSVSDYIVM 190 200 210 220 230 240 FFRFVTAGDI RTRADFFEPF ITGLSNATVD QFCKSSVEPM GEESDHIHIT ALSDALGVAI 250 260 270 280 290 300 RVVYLDRSSC DSGGVTVNHH DFVPVGITNE KDEEASAPFI TLLYRPGHYD ILYPKPSCKV SDNVGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC069471 Genomic DNA. Translation: AAG51478.1. CP002684 Genomic DNA. Translation: AEE30920.1. AY136373 mRNA. Translation: AAM97039.1. BT000164 mRNA. Translation: AAN15483.1. AY084389 mRNA. Translation: AAM60966.1.
IPI	IPI00531741.
PIR	A86407.
RefSeq	NP_564299.1. NM_102577.3.
UniGene	At.43774.
3D structure databases
ProteinModelPortal	Q8LG98.
SMR	Q8LG98. Positions 47-293.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LG98. 2 interactions.
STRING	3702.AT1G28120.1-P.
Protein family/group databases
MEROPS	C65.001.
Proteomic databases
PaxDb	Q8LG98.
PRIDE	Q8LG98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G28120.1; AT1G28120.1; AT1G28120.
GeneID	839705.
KEGG	ath:AT1G28120.
Organism-specific databases
TAIR	At1g28120.
Phylogenomic databases
eggNOG	NOG267426.
HOGENOM	HOG000019496.
InParanoid	Q8LG98.
KO	K09602.
OMA	VVYLRLI.
PhylomeDB	Q8LG98.
ProtClustDB	CLSN2688044.
Gene expression databases
Genevestigator	Q8LG98.
GermOnline	AT1G28120. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003323. OTU. IPR019400. Peptidase_C65_otubain. IPR016615. Ubiquitin_thioesterase_Otubain. [Graphical view]
Pfam	PF10275. Peptidase_C65. 1 hit. [Graphical view]
PIRSF	PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTUBL_ARATH

Accession

Primary (citable) accession number: Q8LG98
Secondary accession number(s): Q9C7E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program