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Protein

Ubiquitin thioesterase otubain-like

Gene

At1g28120

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891By similarity
Active sitei92 – 921NucleophileBy similarity
Active sitei288 – 2881By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT1G28120-MONOMER.

Protein family/group databases

MEROPSiC65.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase otubain-like (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like
Gene namesi
Ordered Locus Names:At1g28120
ORF Names:F13K9.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G28120.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Ubiquitin thioesterase otubain-likePRO_0000221014Add
BLAST

Proteomic databases

PaxDbiQ8LG98.
PRIDEiQ8LG98.

Expressioni

Gene expression databases

ExpressionAtlasiQ8LG98. baseline and differential.
GenevestigatoriQ8LG98.

Interactioni

Protein-protein interaction databases

BioGridi24940. 2 interactions.
IntActiQ8LG98. 2 interactions.
MINTiMINT-8063619.
STRINGi3702.AT1G28120.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LG98.
SMRiQ8LG98. Positions 47-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 295215OTUPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C65 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267426.
HOGENOMiHOG000019496.
InParanoidiQ8LG98.
KOiK09602.
OMAiRVEYMDR.
PhylomeDBiQ8LG98.

Family and domain databases

InterProiIPR003323. OTU.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LG98-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQNQIDMVKD EAEVAASISA IKGEEWGNCS SVEDQPSFQE EEAAKVPYVG
60 70 80 90 100
DKEPLSSLAA EYQSGSPILL EKIKILDSQY IGIRRTRGDG NCFFRSFMFS
110 120 130 140 150
YLEHILESQD RAEVDRIKVN VEKCRKTLQN LGYTDFTFED FFALFLEQLD
160 170 180 190 200
DILQGTEESI SYDELVNRSR DQSVSDYIVM FFRFVTAGDI RTRADFFEPF
210 220 230 240 250
ITGLSNATVD QFCKSSVEPM GEESDHIHIT ALSDALGVAI RVVYLDRSSC
260 270 280 290 300
DSGGVTVNHH DFVPVGITNE KDEEASAPFI TLLYRPGHYD ILYPKPSCKV

SDNVGK
Length:306
Mass (Da):34,434
Last modified:March 1, 2004 - v2
Checksum:i7D8822E69806AC87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51I → N in AAM60966. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069471 Genomic DNA. Translation: AAG51478.1.
CP002684 Genomic DNA. Translation: AEE30920.1.
AY136373 mRNA. Translation: AAM97039.1.
BT000164 mRNA. Translation: AAN15483.1.
AY084389 mRNA. Translation: AAM60966.1.
PIRiA86407.
RefSeqiNP_564299.1. NM_102577.3.
UniGeneiAt.43774.

Genome annotation databases

EnsemblPlantsiAT1G28120.1; AT1G28120.1; AT1G28120.
GeneIDi839705.
KEGGiath:AT1G28120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069471 Genomic DNA. Translation: AAG51478.1.
CP002684 Genomic DNA. Translation: AEE30920.1.
AY136373 mRNA. Translation: AAM97039.1.
BT000164 mRNA. Translation: AAN15483.1.
AY084389 mRNA. Translation: AAM60966.1.
PIRiA86407.
RefSeqiNP_564299.1. NM_102577.3.
UniGeneiAt.43774.

3D structure databases

ProteinModelPortaliQ8LG98.
SMRiQ8LG98. Positions 47-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24940. 2 interactions.
IntActiQ8LG98. 2 interactions.
MINTiMINT-8063619.
STRINGi3702.AT1G28120.1-P.

Protein family/group databases

MEROPSiC65.002.

Proteomic databases

PaxDbiQ8LG98.
PRIDEiQ8LG98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G28120.1; AT1G28120.1; AT1G28120.
GeneIDi839705.
KEGGiath:AT1G28120.

Organism-specific databases

TAIRiAT1G28120.

Phylogenomic databases

eggNOGiNOG267426.
HOGENOMiHOG000019496.
InParanoidiQ8LG98.
KOiK09602.
OMAiRVEYMDR.
PhylomeDBiQ8LG98.

Enzyme and pathway databases

BioCyciARA:AT1G28120-MONOMER.

Gene expression databases

ExpressionAtlasiQ8LG98. baseline and differential.
GenevestigatoriQ8LG98.

Family and domain databases

InterProiIPR003323. OTU.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiOTUBL_ARATH
AccessioniPrimary (citable) accession number: Q8LG98
Secondary accession number(s): Q9C7E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: January 7, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.