Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8LG98

- OTUBL_ARATH

UniProt

Q8LG98 - OTUBL_ARATH

Protein

Ubiquitin thioesterase otubain-like

Gene

At1g28120

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Possible hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891By similarity
    Active sitei92 – 921NucleophileBy similarity
    Active sitei288 – 2881By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. omega peptidase activity Source: InterPro

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT1G28120-MONOMER.

    Protein family/group databases

    MEROPSiC65.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase otubain-like (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme otubain-like
    Ubiquitin-specific-processing protease otubain-like
    Gene namesi
    Ordered Locus Names:At1g28120
    ORF Names:F13K9.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G28120.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306Ubiquitin thioesterase otubain-likePRO_0000221014Add
    BLAST

    Proteomic databases

    PaxDbiQ8LG98.
    PRIDEiQ8LG98.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8LG98.
    GenevestigatoriQ8LG98.

    Interactioni

    Protein-protein interaction databases

    BioGridi24940. 2 interactions.
    IntActiQ8LG98. 2 interactions.
    MINTiMINT-8063619.
    STRINGi3702.AT1G28120.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LG98.
    SMRiQ8LG98. Positions 47-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 295215OTUPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C65 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG267426.
    HOGENOMiHOG000019496.
    InParanoidiQ8LG98.
    KOiK09602.
    OMAiVEPMYKE.
    PhylomeDBiQ8LG98.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view]
    PfamiPF10275. Peptidase_C65. 1 hit.
    [Graphical view]
    PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8LG98-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNQIDMVKD EAEVAASISA IKGEEWGNCS SVEDQPSFQE EEAAKVPYVG    50
    DKEPLSSLAA EYQSGSPILL EKIKILDSQY IGIRRTRGDG NCFFRSFMFS 100
    YLEHILESQD RAEVDRIKVN VEKCRKTLQN LGYTDFTFED FFALFLEQLD 150
    DILQGTEESI SYDELVNRSR DQSVSDYIVM FFRFVTAGDI RTRADFFEPF 200
    ITGLSNATVD QFCKSSVEPM GEESDHIHIT ALSDALGVAI RVVYLDRSSC 250
    DSGGVTVNHH DFVPVGITNE KDEEASAPFI TLLYRPGHYD ILYPKPSCKV 300
    SDNVGK 306
    Length:306
    Mass (Da):34,434
    Last modified:March 1, 2004 - v2
    Checksum:i7D8822E69806AC87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51I → N in AAM60966. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC069471 Genomic DNA. Translation: AAG51478.1.
    CP002684 Genomic DNA. Translation: AEE30920.1.
    AY136373 mRNA. Translation: AAM97039.1.
    BT000164 mRNA. Translation: AAN15483.1.
    AY084389 mRNA. Translation: AAM60966.1.
    PIRiA86407.
    RefSeqiNP_564299.1. NM_102577.3.
    UniGeneiAt.43774.

    Genome annotation databases

    EnsemblPlantsiAT1G28120.1; AT1G28120.1; AT1G28120.
    GeneIDi839705.
    KEGGiath:AT1G28120.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC069471 Genomic DNA. Translation: AAG51478.1 .
    CP002684 Genomic DNA. Translation: AEE30920.1 .
    AY136373 mRNA. Translation: AAM97039.1 .
    BT000164 mRNA. Translation: AAN15483.1 .
    AY084389 mRNA. Translation: AAM60966.1 .
    PIRi A86407.
    RefSeqi NP_564299.1. NM_102577.3.
    UniGenei At.43774.

    3D structure databases

    ProteinModelPortali Q8LG98.
    SMRi Q8LG98. Positions 47-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24940. 2 interactions.
    IntActi Q8LG98. 2 interactions.
    MINTi MINT-8063619.
    STRINGi 3702.AT1G28120.1-P.

    Protein family/group databases

    MEROPSi C65.001.

    Proteomic databases

    PaxDbi Q8LG98.
    PRIDEi Q8LG98.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G28120.1 ; AT1G28120.1 ; AT1G28120 .
    GeneIDi 839705.
    KEGGi ath:AT1G28120.

    Organism-specific databases

    TAIRi AT1G28120.

    Phylogenomic databases

    eggNOGi NOG267426.
    HOGENOMi HOG000019496.
    InParanoidi Q8LG98.
    KOi K09602.
    OMAi VEPMYKE.
    PhylomeDBi Q8LG98.

    Enzyme and pathway databases

    BioCyci ARA:AT1G28120-MONOMER.

    Gene expression databases

    ArrayExpressi Q8LG98.
    Genevestigatori Q8LG98.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view ]
    Pfami PF10275. Peptidase_C65. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiOTUBL_ARATH
    AccessioniPrimary (citable) accession number: Q8LG98
    Secondary accession number(s): Q9C7E1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3