Tonoplast dicarboxylate transporter - Arabidopsis thaliana (Mouse-ear cress)

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Q8LG88 (TDT_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tonoplast dicarboxylate transporter
Short name=AttDT

Alternative name(s):
Sodium-dicarboxylate cotransporter-like
Short name=AtSDAT
Vacuolar malate transporter

Gene names

Name:	TDT
Synonyms:	SDAT
Ordered Locus Names:	At5g47560
ORF Names:	MNJ7.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative carrier protein indirectly involved in the uptake of malate and fumarate to the vacuole, probably by regulating the energization across the tonoplast. Uptake of malate to vacuoles is inhibited by citrate and by the uncoupler carbonyl-cyanide m-chlorophenylhydrazone, but seems to be not affected by sodium. Critical for pH homeostasis. Ref.1 Ref.7
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Ref.1
Tissue specificity	Expressed in leaves, stems and flowers. Ref.1
Induction	Induced by malate and by acidification of the cytoplasm. Ref.1 Ref.7
Sequence similarities	Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. [View classification]
Sequence caution	The sequence BAB09082.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAC19843.1 differs from that shown. Reason: Frameshift at positions 475 and 479.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of intracellular pH Traceable author statement Ref.7. Source: TAIR sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	malate transmembrane transporter activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 540

540

Tonoplast dicarboxylate transporter

PRO_0000172526

Regions

Transmembrane

46 – 66

Helical; Potential

Transmembrane

75 – 95

Helical; Potential

Transmembrane

123 – 143

Helical; Potential

Transmembrane

164 – 184

Helical; Potential

Transmembrane

219 – 239

Helical; Potential

Transmembrane

265 – 285

Helical; Potential

Transmembrane

311 – 331

Helical; Potential

Transmembrane

353 – 373

Helical; Potential

Transmembrane

392 – 412

Helical; Potential

Transmembrane

419 – 439

Helical; Potential

Transmembrane

466 – 486

Helical; Potential

Transmembrane

520 – 540

Helical; Potential

Compositional bias

201 – 204

Poly-Ser

Experimental info

Sequence conflict

310

D → E in AAM60982. Ref.6

Sequence conflict

407

R → P in CAC19843. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q8LG88 [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 4BF0107A9C4B3B74
Show »

FASTA

540

58,098

        10         20         30         40         50         60 
MNGGDVTVAG SDDLKSPLLP VVHNDEPFER QTVGQQLRTI FTPKNCYIAL GPLLCAVVCL 

        70         80         90        100        110        120 
CVDLGGDETT TARNMLGVLV WMFAWWLTEA VPMPITSMTP LFLFPLFGIS AADDVANSYM 

       130        140        150        160        170        180 
DDVISLVLGS FILALAVEHY NIHRRLALNI TLVFCVEPLN APLLLLGICA TTAFVSMWMH 

       190        200        210        220        230        240 
NVAAAVMMMP VATGILQRLP SSSSTTEVVH PAVGKFSRAV VLGVIYSAAV GGMSTLTGTG 

       250        260        270        280        290        300 
VNLILVGMWK SYFPEADPIS FSQWFFFGFP LALCIFVVLW CVLCVMYCPK GAGQALSPYL 

       310        320        330        340        350        360 
HKSHLRRELD LLGPMNFAEK MVLAVFGGLV VLWMTRNITD DIPGWGRIFA GRAGDGTVSV 

       370        380        390        400        410        420 
MMATLLFIIP SNIKKGEKLM DWNKCKKLPW NIVLLLGAGF AIADGVRTSG LAEVLSKGLV 

       430        440        450        460        470        480 
FLETAPYWAI APTVCLIAAT ITEFTSNNAT TTLLVPLLIE IAKNMGIHPL LLMVPGAIGA 

       490        500        510        520        530        540 
QFAFLLPTGT PSNVVGFTTG HIEIKDMIKT GLPLKIAGTI FLSILMPTLG AYVFASMGGV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The plant homolog to the human sodium/dicarboxylic cotransporter is the vacuolar malate carrier." Emmerlich V., Linka N., Reinhold T., Hurth M.A., Traub M., Martinoia E., Neuhaus H.E. Proc. Natl. Acad. Sci. U.S.A. 100:11122-11126(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[2]	"Sodium sulfate or dicarboxylate transporter form Arabidopsis thaliana." Saito K., Takahashi H. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Impaired pH homeostasis in Arabidopsis lacking the vacuolar dicarboxylate transporter and analysis of carboxylic acid transport across the tonoplast." Hurth M.A., Suh S.J., Kretzschmar T., Geis T., Bregante M., Gambale F., Martinoia E., Neuhaus H.E. Plant Physiol. 137:901-910(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223445 mRNA. Translation: CAC19843.1. Frameshift. AB043024 mRNA. Translation: BAA96091.1. AB025628 Genomic DNA. Translation: BAB09082.1. Sequence problems. CP002688 Genomic DNA. Translation: AED95532.1. AY072145 mRNA. Translation: AAL59967.1. AY133792 mRNA. Translation: AAM91726.1. AY084408 mRNA. Translation: AAM60982.1.
IPI	IPI00534224.
RefSeq	NP_199567.1. NM_124129.3.
UniGene	At.21038. At.68515. At.71618.
3D structure databases
SMR	Q8LG88. Positions 42-531.
ModBase	Search...
Protein family/group databases
TCDB	2.A.47.1.6. divalent anion:Na+ symporter (DASS) family.
Proteomic databases
PaxDb	Q8LG88.
PRIDE	Q8LG88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G47560.1; AT5G47560.1; AT5G47560.
GeneID	834806.
KEGG	ath:AT5G47560.
Organism-specific databases
GeneFarm	4980.
TAIR	At5g47560.
Phylogenomic databases
eggNOG	COG0471.
HOGENOM	HOG000278432.
InParanoid	Q8LG88.
KO	K14445.
OMA	ELKLINW.
PhylomeDB	Q8LG88.
ProtClustDB	CLSN2686388.
Gene expression databases
ArrayExpress	Q8LG88.
Genevestigator	Q8LG88.
GermOnline	AT5G47560. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001898. Na/sul_symport. [Graphical view]
Pfam	PF00939. Na_sulph_symp. 1 hit. [Graphical view]
TIGRFAMs	TIGR00785. dass. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TDT_ARATH

Accession

Primary (citable) accession number: Q8LG88
Secondary accession number(s): Q9FEH9, Q9FGK1, Q9MAW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents