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Q8LG77

- IDH6_ARATH

UniProt

Q8LG77 - IDH6_ARATH

Protein

Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial

Gene

IDH6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei127 – 1271SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Sitei165 – 1651Critical for catalysisBy similarity
    Sitei212 – 2121Critical for catalysisBy similarity
    Metal bindingi245 – 2451Magnesium or manganeseBy similarity
    Binding sitei245 – 2451SubstrateBy similarity
    Metal bindingi269 – 2691Magnesium or manganeseBy similarity
    Metal bindingi273 – 2731Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: TAIR
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: TAIR
    2. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    IDH-VI
    Isocitric dehydrogenase 6
    NAD(+)-specific ICDH 6
    Gene namesi
    Name:IDH6
    Ordered Locus Names:At3g09810, At3g09805
    ORF Names:F8A24.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G09810.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4444MitochondrionSequence AnalysisAdd
    BLAST
    Chaini45 – 374330Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrialPRO_0000271292Add
    BLAST

    Proteomic databases

    PaxDbiQ8LG77.
    PRIDEiQ8LG77.

    Expressioni

    Tissue specificityi

    Ubiquitous. Predominantly expressed in leaves.1 Publication

    Gene expression databases

    ArrayExpressiQ8LG77.
    GenevestigatoriQ8LG77.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LG77.
    SMRiQ8LG77. Positions 47-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    InParanoidiQ8LG77.
    KOiK00030.
    OMAiRKASMRV.
    PhylomeDBiQ8LG77.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LG77-1 [UniParc]FASTAAdd to Basket

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    MTMTAFLARR LIGNGSSQIL GTSSSSSGPF ISVSRAFFSS STPIKATLFP    50
    GDGIGPEIAE SVKQVFTAAD VVIDWDEQFV GTEVDPRTNS FLTWDNLQSV 100
    LKNKVGLKGP MATPIGKGHR SLNLTLRKEL NLYANVRPCY SLPGYKTRYD 150
    DVDLITIREN TEGEYSGLEH QVVKGVVESL KIITRKASMR VAEYAFLYAK 200
    THGRKKVSAI HKANIMQKTD GLFLQCCDEV AAKYPEIYYE KVVIDNCCMM 250
    LVKNPALFDV LVMPNLYGDI ISDLCAGLVG GLGLTPSMNI GEDGIALAEA 300
    VHGSAPDIAG MNLANPTALL LSGVMMLRHL KLNKQAEQIH SAIINTIAEG 350
    KYRTADLGGS STTTDFTKAI CDHL 374
    Length:374
    Mass (Da):40,576
    Last modified:January 9, 2007 - v2
    Checksum:i3587127E0EAB87A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31M → I in AAM60999. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC015985 Genomic DNA. Translation: AAF23254.1.
    CP002686 Genomic DNA. Translation: AEE74815.1.
    AF324664 mRNA. Translation: AAG40015.1.
    AF327427 mRNA. Translation: AAG42017.1.
    AF339723 mRNA. Translation: AAK00405.1.
    AK176269 mRNA. Translation: BAD44032.1.
    AK228113 mRNA. Translation: BAF00071.1.
    AY084425 mRNA. Translation: AAM60999.1.
    RefSeqiNP_850549.1. NM_180218.1.
    UniGeneiAt.22515.

    Genome annotation databases

    EnsemblPlantsiAT3G09810.1; AT3G09810.1; AT3G09810.
    GeneIDi820139.
    KEGGiath:AT3G09810.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC015985 Genomic DNA. Translation: AAF23254.1 .
    CP002686 Genomic DNA. Translation: AEE74815.1 .
    AF324664 mRNA. Translation: AAG40015.1 .
    AF327427 mRNA. Translation: AAG42017.1 .
    AF339723 mRNA. Translation: AAK00405.1 .
    AK176269 mRNA. Translation: BAD44032.1 .
    AK228113 mRNA. Translation: BAF00071.1 .
    AY084425 mRNA. Translation: AAM60999.1 .
    RefSeqi NP_850549.1. NM_180218.1.
    UniGenei At.22515.

    3D structure databases

    ProteinModelPortali Q8LG77.
    SMRi Q8LG77. Positions 47-374.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8LG77.
    PRIDEi Q8LG77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G09810.1 ; AT3G09810.1 ; AT3G09810 .
    GeneIDi 820139.
    KEGGi ath:AT3G09810.

    Organism-specific databases

    TAIRi AT3G09810.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    InParanoidi Q8LG77.
    KOi K00030.
    OMAi RKASMRV.
    PhylomeDBi Q8LG77.

    Gene expression databases

    ArrayExpressi Q8LG77.
    Genevestigatori Q8LG77.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    7. "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana."
      Lin M., Behal R.H., Oliver D.J.
      Plant Sci. 166:983-988(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: GENE FAMILY.
    8. "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs."
      Lemaitre T., Hodges M.
      Plant Cell Physiol. 47:634-643(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIDH6_ARATH
    AccessioniPrimary (citable) accession number: Q8LG77
    Secondary accession number(s): Q9SF84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3