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Q8LG64 (CKB22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase B2-2

Short name=CDKB2;2
EC=2.7.11.22
EC=2.7.11.23
Gene names
Name:CDKB2-2
Ordered Locus Names:At1g20930
ORF Names:F9H16.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Tissue specificity

Expressed in flowers. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAM61014.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Cyclin-dependent kinase B2-2
PRO_0000293116

Regions

Domain16 – 306291Protein kinase
Nucleotide binding22 – 309ATP By similarity

Sites

Active site1471Proton acceptor By similarity
Binding site451ATP By similarity

Amino acid modifications

Modified residue271Phosphotyrosine By similarity
Modified residue1811Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8LG64 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 3C1A33C9FD94F36F

FASTA31535,847
        10         20         30         40         50         60 
MDNNGVKPAV SAMEAFEKLE KVGEGTYGKV YRAREKATGM IVALKKTRLH EDEEGVPPTT 

        70         80         90        100        110        120 
LREISILRML ARDPHIVRLM DVKQGINKEG KTVLYLVFEY VDTDLKKFIR SFRQAGQNIP 

       130        140        150        160        170        180 
QNTVKCLMYQ LCKGMAFCHG HGVLHRDLKP HNLLMDRKTM TLKIADLGLA RAFTLPMKKY 

       190        200        210        220        230        240 
THEILTLWYR APEVLLGATH YSTGVDMWSV GCIFAELVTK QAIFAGDSEL QQLLRIFRLL 

       250        260        270        280        290        300 
GTPNEEVWPG VSKLKDWHEY PQWKPLSLST AVPNLDEAGL DLLSKMLEYE PAKRISAKKA 

       310 
MEHPYFDDLP DKSSL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Ecker J.R.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Genome-wide analysis of core cell cycle genes in Arabidopsis."
Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Cell cycle regulation in plant development."
Inze D., de Veylder L.
Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC007369 Genomic DNA. Translation: AAD30597.1.
CP002684 Genomic DNA. Translation: AEE30043.1.
BT024780 mRNA. Translation: ABD59118.1.
AK229456 mRNA. Translation: BAF01314.1.
AY084441 mRNA. Translation: AAM61014.1. Different initiation.
PIRB86342.
RefSeqNP_173517.1. NM_101946.3.
UniGeneAt.20756.
At.41679.

3D structure databases

ProteinModelPortalQ8LG64.
SMRQ8LG64. Positions 13-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid23926. 18 interactions.
IntActQ8LG64. 16 interactions.
STRING3702.AT1G20930.1-P.

Proteomic databases

PaxDbQ8LG64.
PRIDEQ8LG64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G20930.1; AT1G20930.1; AT1G20930.
GeneID838687.
KEGGath:AT1G20930.

Organism-specific databases

GeneFarm3279. 107.
TAIRAT1G20930.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ8LG64.
KOK07760.
OMADWHEYPQ.
PhylomeDBQ8LG64.
ProtClustDBCLSN2679448.

Enzyme and pathway databases

BioCycARA:AT1G20930-MONOMER.

Gene expression databases

GenevestigatorQ8LG64.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCKB22_ARATH
AccessionPrimary (citable) accession number: Q8LG64
Secondary accession number(s): Q9SYP4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names