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Q8LG64

- CKB22_ARATH

UniProt

Q8LG64 - CKB22_ARATH

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Protein

Cyclin-dependent kinase B2-2

Gene

CDKB2-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451ATPPROSITE-ProRule annotation
Active sitei147 – 1471Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 309ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: TAIR
  3. protein serine/threonine kinase activity Source: TAIR
  4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. hormone-mediated signaling pathway Source: TAIR
  2. protein autophosphorylation Source: TAIR
  3. regulation of G2/M transition of mitotic cell cycle Source: TAIR
  4. regulation of meristem structural organization Source: TAIR
  5. regulation of mitotic cell cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G20930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B2-2 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB2;2
Gene namesi
Name:CDKB2-2
Ordered Locus Names:At1g20930
ORF Names:F9H16.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G20930.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Cyclin-dependent kinase B2-2PRO_0000293116Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271PhosphotyrosineBy similarity
Modified residuei181 – 1811PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8LG64.
PRIDEiQ8LG64.

Expressioni

Tissue specificityi

Expressed in flowers.1 Publication

Gene expression databases

GenevestigatoriQ8LG64.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAM4P258542EBI-1235761,EBI-1235664
CAM6Q035092EBI-1235761,EBI-1236097

Protein-protein interaction databases

BioGridi23926. 18 interactions.
IntActiQ8LG64. 16 interactions.
STRINGi3702.AT1G20930.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LG64.
SMRiQ8LG64. Positions 13-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 306291Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ8LG64.
KOiK07760.
OMAiKATGMIV.
PhylomeDBiQ8LG64.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LG64-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNNGVKPAV SAMEAFEKLE KVGEGTYGKV YRAREKATGM IVALKKTRLH
60 70 80 90 100
EDEEGVPPTT LREISILRML ARDPHIVRLM DVKQGINKEG KTVLYLVFEY
110 120 130 140 150
VDTDLKKFIR SFRQAGQNIP QNTVKCLMYQ LCKGMAFCHG HGVLHRDLKP
160 170 180 190 200
HNLLMDRKTM TLKIADLGLA RAFTLPMKKY THEILTLWYR APEVLLGATH
210 220 230 240 250
YSTGVDMWSV GCIFAELVTK QAIFAGDSEL QQLLRIFRLL GTPNEEVWPG
260 270 280 290 300
VSKLKDWHEY PQWKPLSLST AVPNLDEAGL DLLSKMLEYE PAKRISAKKA
310
MEHPYFDDLP DKSSL
Length:315
Mass (Da):35,847
Last modified:July 10, 2007 - v2
Checksum:i3C1A33C9FD94F36F
GO

Sequence cautioni

The sequence AAM61014.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007369 Genomic DNA. Translation: AAD30597.1.
CP002684 Genomic DNA. Translation: AEE30043.1.
BT024780 mRNA. Translation: ABD59118.1.
AK229456 mRNA. Translation: BAF01314.1.
AY084441 mRNA. Translation: AAM61014.1. Different initiation.
PIRiB86342.
RefSeqiNP_173517.1. NM_101946.3.
UniGeneiAt.20756.
At.41679.

Genome annotation databases

EnsemblPlantsiAT1G20930.1; AT1G20930.1; AT1G20930.
GeneIDi838687.
KEGGiath:AT1G20930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC007369 Genomic DNA. Translation: AAD30597.1 .
CP002684 Genomic DNA. Translation: AEE30043.1 .
BT024780 mRNA. Translation: ABD59118.1 .
AK229456 mRNA. Translation: BAF01314.1 .
AY084441 mRNA. Translation: AAM61014.1 . Different initiation.
PIRi B86342.
RefSeqi NP_173517.1. NM_101946.3.
UniGenei At.20756.
At.41679.

3D structure databases

ProteinModelPortali Q8LG64.
SMRi Q8LG64. Positions 13-309.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 23926. 18 interactions.
IntActi Q8LG64. 16 interactions.
STRINGi 3702.AT1G20930.1-P.

Proteomic databases

PaxDbi Q8LG64.
PRIDEi Q8LG64.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G20930.1 ; AT1G20930.1 ; AT1G20930 .
GeneIDi 838687.
KEGGi ath:AT1G20930.

Organism-specific databases

GeneFarmi 3279. 107.
TAIRi AT1G20930.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q8LG64.
KOi K07760.
OMAi KATGMIV.
PhylomeDBi Q8LG64.

Enzyme and pathway databases

BioCyci ARA:AT1G20930-MONOMER.

Gene expression databases

Genevestigatori Q8LG64.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
    Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
    J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
    Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
    Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Cell cycle regulation in plant development."
    Inze D., de Veylder L.
    Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCKB22_ARATH
AccessioniPrimary (citable) accession number: Q8LG64
Secondary accession number(s): Q9SYP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: October 29, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3