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Q8LG64

- CKB22_ARATH

UniProt

Q8LG64 - CKB22_ARATH

Protein

Cyclin-dependent kinase B2-2

Gene

CDKB2-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451ATPPROSITE-ProRule annotation
    Active sitei147 – 1471Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 309ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: TAIR
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: TAIR
    5. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. hormone-mediated signaling pathway Source: TAIR
    2. protein autophosphorylation Source: TAIR
    3. regulation of G2/M transition of mitotic cell cycle Source: TAIR
    4. regulation of meristem structural organization Source: TAIR
    5. regulation of mitotic cell cycle Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G20930-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase B2-2 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKB2;2
    Gene namesi
    Name:CDKB2-2
    Ordered Locus Names:At1g20930
    ORF Names:F9H16.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G20930.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Cyclin-dependent kinase B2-2PRO_0000293116Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271PhosphotyrosineBy similarity
    Modified residuei181 – 1811PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8LG64.
    PRIDEiQ8LG64.

    Expressioni

    Tissue specificityi

    Expressed in flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ8LG64.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAM4P258542EBI-1235761,EBI-1235664
    CAM6Q035092EBI-1235761,EBI-1236097

    Protein-protein interaction databases

    BioGridi23926. 18 interactions.
    IntActiQ8LG64. 16 interactions.
    STRINGi3702.AT1G20930.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LG64.
    SMRiQ8LG64. Positions 13-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 306291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ8LG64.
    KOiK07760.
    OMAiKATGMIV.
    PhylomeDBiQ8LG64.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8LG64-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNNGVKPAV SAMEAFEKLE KVGEGTYGKV YRAREKATGM IVALKKTRLH    50
    EDEEGVPPTT LREISILRML ARDPHIVRLM DVKQGINKEG KTVLYLVFEY 100
    VDTDLKKFIR SFRQAGQNIP QNTVKCLMYQ LCKGMAFCHG HGVLHRDLKP 150
    HNLLMDRKTM TLKIADLGLA RAFTLPMKKY THEILTLWYR APEVLLGATH 200
    YSTGVDMWSV GCIFAELVTK QAIFAGDSEL QQLLRIFRLL GTPNEEVWPG 250
    VSKLKDWHEY PQWKPLSLST AVPNLDEAGL DLLSKMLEYE PAKRISAKKA 300
    MEHPYFDDLP DKSSL 315
    Length:315
    Mass (Da):35,847
    Last modified:July 10, 2007 - v2
    Checksum:i3C1A33C9FD94F36F
    GO

    Sequence cautioni

    The sequence AAM61014.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007369 Genomic DNA. Translation: AAD30597.1.
    CP002684 Genomic DNA. Translation: AEE30043.1.
    BT024780 mRNA. Translation: ABD59118.1.
    AK229456 mRNA. Translation: BAF01314.1.
    AY084441 mRNA. Translation: AAM61014.1. Different initiation.
    PIRiB86342.
    RefSeqiNP_173517.1. NM_101946.3.
    UniGeneiAt.20756.
    At.41679.

    Genome annotation databases

    EnsemblPlantsiAT1G20930.1; AT1G20930.1; AT1G20930.
    GeneIDi838687.
    KEGGiath:AT1G20930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007369 Genomic DNA. Translation: AAD30597.1 .
    CP002684 Genomic DNA. Translation: AEE30043.1 .
    BT024780 mRNA. Translation: ABD59118.1 .
    AK229456 mRNA. Translation: BAF01314.1 .
    AY084441 mRNA. Translation: AAM61014.1 . Different initiation.
    PIRi B86342.
    RefSeqi NP_173517.1. NM_101946.3.
    UniGenei At.20756.
    At.41679.

    3D structure databases

    ProteinModelPortali Q8LG64.
    SMRi Q8LG64. Positions 13-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23926. 18 interactions.
    IntActi Q8LG64. 16 interactions.
    STRINGi 3702.AT1G20930.1-P.

    Proteomic databases

    PaxDbi Q8LG64.
    PRIDEi Q8LG64.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G20930.1 ; AT1G20930.1 ; AT1G20930 .
    GeneIDi 838687.
    KEGGi ath:AT1G20930.

    Organism-specific databases

    GeneFarmi 3279. 107.
    TAIRi AT1G20930.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q8LG64.
    KOi K07760.
    OMAi KATGMIV.
    PhylomeDBi Q8LG64.

    Enzyme and pathway databases

    BioCyci ARA:AT1G20930-MONOMER.

    Gene expression databases

    Genevestigatori Q8LG64.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
      Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
      J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
      Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
      Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Cell cycle regulation in plant development."
      Inze D., de Veylder L.
      Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiCKB22_ARATH
    AccessioniPrimary (citable) accession number: Q8LG64
    Secondary accession number(s): Q9SYP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3