Cyclin-dependent kinase B2-2 - Arabidopsis thaliana (Mouse-ear cress)

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Q8LG64 (CKB22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase B2-2
Short name=CDKB2;2
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	CDKB2-2
Ordered Locus Names:	At1g20930
ORF Names:	F9H16.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Tissue specificity	Expressed in flowers. Ref.6
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAM61014.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hormone-mediated signaling pathway Inferred from expression pattern PubMed 18223038. Source: TAIR protein autophosphorylation Inferred from direct assay PubMed 21477822. Source: TAIR regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 18223038. Source: TAIR regulation of meristem structural organization Inferred from mutant phenotype PubMed 18223038. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Traceable author statement Ref.6. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAM1	P25854	2	EBI-1235761,EBI-1235664
CAM6	Q03509	2	EBI-1235761,EBI-1236097

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 315

315

Cyclin-dependent kinase B2-2

PRO_0000293116

Regions

Domain

16 – 306

291

Protein kinase

Nucleotide binding

22 – 30

ATP By similarity

Sites

Active site

147

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

181

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8LG64 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 3C1A33C9FD94F36F
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FASTA

315

35,847

        10         20         30         40         50         60 
MDNNGVKPAV SAMEAFEKLE KVGEGTYGKV YRAREKATGM IVALKKTRLH EDEEGVPPTT 

        70         80         90        100        110        120 
LREISILRML ARDPHIVRLM DVKQGINKEG KTVLYLVFEY VDTDLKKFIR SFRQAGQNIP 

       130        140        150        160        170        180 
QNTVKCLMYQ LCKGMAFCHG HGVLHRDLKP HNLLMDRKTM TLKIADLGLA RAFTLPMKKY 

       190        200        210        220        230        240 
THEILTLWYR APEVLLGATH YSTGVDMWSV GCIFAELVTK QAIFAGDSEL QQLLRIFRLL 

       250        260        270        280        290        300 
GTPNEEVWPG VSKLKDWHEY PQWKPLSLST AVPNLDEAGL DLLSKMLEYE PAKRISAKKA 

       310 
MEHPYFDDLP DKSSL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis." Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L. J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007369 Genomic DNA. Translation: AAD30597.1. CP002684 Genomic DNA. Translation: AEE30043.1. BT024780 mRNA. Translation: ABD59118.1. AK229456 mRNA. Translation: BAF01314.1. AY084441 mRNA. Translation: AAM61014.1. Different initiation.
IPI	IPI00529080.
PIR	B86342.
RefSeq	NP_173517.1. NM_101946.3.
UniGene	At.20756. At.41679.
3D structure databases
ProteinModelPortal	Q8LG64.
SMR	Q8LG64. Positions 13-309.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LG64. 16 interactions.
STRING	3702.AT1G20930.1-P.
Proteomic databases
PaxDb	Q8LG64.
PRIDE	Q8LG64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G20930.1; AT1G20930.1; AT1G20930.
GeneID	838687.
KEGG	ath:AT1G20930.
Organism-specific databases
GeneFarm	3279. 107.
TAIR	At1g20930.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q8LG64.
KO	K00924.
OMA	VSAMEAF.
PhylomeDB	Q8LG64.
ProtClustDB	CLSN2679448.
Gene expression databases
Genevestigator	Q8LG64.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CKB22_ARATH

Accession

Primary (citable) accession number: Q8LG64
Secondary accession number(s): Q9SYP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 29, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents