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Q8LG58 (XTH16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 16

Short name=At-XTH16
Short name=XTH-16
EC=2.4.1.207
Gene names
Name:XTH16
Ordered Locus Names:At3g23730
ORF Names:MYM9.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 291267Probable xyloglucan endotransglucosylase/hydrolase protein 16
PRO_0000011816

Sites

Active site1011Nucleophile By similarity
Active site1051Proton donor By similarity

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2561R → I in AAM61021. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8LG58 [UniParc].

Last modified November 28, 2003. Version 2.
Checksum: 3AE0B913F783C764

FASTA29133,147
        10         20         30         40         50         60 
MGRILNRTVL MTLLVVTMAG TAFSGSFNEE FDLTWGEHRG KIFSGGKMLS LSLDRVSGSG 

        70         80         90        100        110        120 
FKSKKEYLFG RIDMQLKLVA GNSAGTVTAY YLSSEGPTHD EIDFEFLGNE TGKPYVLHTN 

       130        140        150        160        170        180 
VFAQGKGNRE QQFYLWFDPT KNFHTYSLVW RPQHIIFMVD NVPIRVFNNA EQLGVPFPKN 

       190        200        210        220        230        240 
QPMKIYSSLW NADDWATRGG LVKTDWSKAP FTAYYRGFNA AACTVSSGSS FCDPKFKSSF 

       250        260        270        280        290 
TNGESQVANE LNAYGRRRLR WVQKYFMIYD YCSDLKRFPQ GFPPECRKSR V 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000377 Genomic DNA. Translation: BAB01849.1.
CP002686 Genomic DNA. Translation: AEE76807.1.
AY084449 mRNA. Translation: AAM61021.1.
RefSeqNP_566738.1. NM_113277.2.
UniGeneAt.26810.

3D structure databases

ProteinModelPortalQ8LG58.
SMRQ8LG58. Positions 26-290.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ8LG58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G23730.1; AT3G23730.1; AT3G23730.
GeneID821955.
KEGGath:AT3G23730.

Organism-specific databases

TAIRAT3G23730.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ8LG58.
KOK08235.
OMAGFPPECR.
PhylomeDBQ8LG58.

Enzyme and pathway databases

BioCycARA:AT3G23730-MONOMER.

Gene expression databases

ArrayExpressQ8LG58.
GenevestigatorQ8LG58.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH16_ARATH
AccessionPrimary (citable) accession number: Q8LG58
Secondary accession number(s): Q9LK45
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 14, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names