Reviewed, UniProtKB/Swiss-Prot Q8LFV3 (DOF33_ARATH)

Names and origin

Protein names

Recommended name:
    Dof zinc finger protein DOF3.3
      Short name=AtDOF3.3
Alternative name(s):
    H-protein promoter-binding factor 2a

Gene names

Name:	DOF3.3
Synonyms:	HPPBF-2A
Ordered Locus Names:	At3g47500
ORF Names:	F1P2.50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 Dof-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO2	Q8W420	3	EBI-1536119,EBI-1015688
ADO3	Q9C9W9	1	EBI-1536119,EBI-401228

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Dof zinc finger protein DOF3.3		PRO_0000074279
Regions
	Zinc finger	110 – 164	55	Dof-type
	Compositional bias	291 – 329	39	Pro-rich
Experimental info
	Sequence conflict	88	1	D → E in AAM61191. Ref.4
	Sequence conflict	201	1	Q → H in AAM61191. Ref.4
	Sequence conflict	206	1	Missing Ref.4
	Sequence conflict	210	1	T → A in AAM61191. Ref.4
	Sequence conflict	217	1	E → G in AAM61191. Ref.4
	Sequence conflict	228	1	R → G in AAM61191. Ref.4
	Sequence conflict	270 – 271	2	SV → RI in AAM61191. Ref.4
	Sequence conflict	274	1	A → P in AAM61191. Ref.4
	Sequence conflict	277 – 278	2	Missing in AAM61191. Ref.4
	Sequence conflict	327 – 328	2	LP → ISPS in AAM61191. Ref.4
	Sequence conflict	340	1	C → D in AAM61191. Ref.4
	Sequence conflict	351 – 352	2	HP → RS in AAM61191. Ref.4
	Sequence conflict	356	1	G → E in AAM61191. Ref.4
	Sequence conflict	360 – 362	3	KDN → RDS in AAM61191. Ref.4
	Sequence conflict	369 – 370	2	KA → RT in AAM61191. Ref.4
	Sequence conflict	373	1	V → I in AAM61191. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8LFV3-1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: 6E61FA7A7A8DF1F6	FASTA	448	49,730
	10 20 30 40 50 60 MMMETRDPAI KLFGMKIPFP SVFESAVTVE DDEEDDWSGG DDKSPEKVTP ELSDKNNNNC 70 80 90 100 110 120 NDNSFNNSKP ETLDKEEATS TDQIESSDTP EDNQQTTPDG KTLKKPTKIL PCPRCKSMET 130 140 150 160 170 180 KFCYYNNYNI NQPRHFCKAC QRYWTAGGTM RNVPVGAGRR KNKSSSSHYR HITISEALEA 190 200 210 220 230 240 ARLDPGLQAN TRVLSFGLEA QQQHVAAPMT PVMKLQEDQK VSNGARNRFH GLADQRLVAR 250 260 270 280 290 300 VENGDDCSSG SSVTTSNNHS VDESRAQSGS VVEAQMNNNN NNNMNGYACI PGVPWPYTWN 310 320 330 340 350 360 PAMPPPGFYP PPGYPMPFYP YWTIPMLPPH QSSSPISQKC SNTNSPTLGK HPRDEGSSKK 370 380 390 400 410 420 DNETERKQKA GCVLVPKTLR IDDPNEAAKS SIWTTLGIKN EAMCKAGGMF KGFDHKTKMY 430 440 NNDKAENSPV LSANPAALSR SHNFHEQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the H-protein promoter binding factor from Arabidopsis." Abbaraju H.K.R., Oliver D.J. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The Dof family of plant transcription factors." Yanagisawa S. Trends Plant Sci. 7:555-560(2002) [PubMed: 12475498] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF079503 mRNA. Translation: AAC28390.1. AL132955 Genomic DNA. Translation: CAB61976.1. AY065152 mRNA. Translation: AAL38328.1. AY128757 mRNA. Translation: AAM91157.1. AY084628 mRNA. Translation: AAM61191.1.
IPI	IPI00547330.
PIR	T45710.
RefSeq	NP_190334.1.
UniGene	At.519
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LFV3. 2 interactions.
Genome annotation databases
GeneID	823904.
GenomeReviews	Gene locus AT3G47500 in contig BA000014_GR.
KEGG	ath:AT3G47500.
NMPDR	fig|3702.1.peg.15973.
Organism-specific databases
GeneFarm	4398. 446.
TAIR	At3g47500.
Gene expression databases
ArrayExpress	Q8LFV3.
GermOnline	AT3G47500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003851. Znf_Dof. [Graphical view]
Pfam	PF02701. zf-Dof. 1 hit. [Graphical view]
PROSITE	PS01361. ZF_DOF_1. 1 hit. PS50884. ZF_DOF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOF33_ARATH

Accession

Primary (citable) accession number: Q8LFV3
Secondary accession number(s): O81600

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)