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Q8LFC0

- IDH1_ARATH

UniProt

Q8LFC0 - IDH1_ARATH

Protein

Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial

Gene

IDH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.By similarity

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161SubstrateBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Sitei154 – 1541Critical for catalysisBy similarity
    Sitei201 – 2011Critical for catalysisBy similarity
    Metal bindingi234 – 2341Magnesium or manganeseBy similarity
    Binding sitei234 – 2341SubstrateBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro
    4. zinc ion binding Source: TAIR

    GO - Biological processi

    1. isocitrate metabolic process Source: TAIR
    2. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    IDH-I
    Isocitric dehydrogenase 1
    NAD(+)-specific ICDH 1
    Gene namesi
    Name:IDH1
    Ordered Locus Names:At4g35260
    ORF Names:F23E12.180
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G35260.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
    BLAST
    Chaini26 – 367342Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrialPRO_0000271287Add
    BLAST

    Proteomic databases

    PaxDbiQ8LFC0.
    PRIDEiQ8LFC0.

    Expressioni

    Tissue specificityi

    Ubiquitous. Predominantly expressed in roots, stems and leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ8LFC0.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.

    Protein-protein interaction databases

    BioGridi14961. 4 interactions.
    IntActiQ8LFC0. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LFC0.
    SMRiQ8LFC0. Positions 40-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    InParanoidiQ8LFC0.
    KOiK00030.
    OMAiAMHAPIF.
    PhylomeDBiQ8LFC0.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LFC0-1 [UniParc]FASTAAdd to Basket

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    MSRRSLTLLK NLARNANGSG IQTRSVTYMP RPGDGAPRAV TLIPGDGIGP    50
    LVTNAVEQVM EAMHAPIFFE KYDVHGEMSR VPPEVMESIR KNKVCLKGGL 100
    KTPVGGGVSS LNVQLRKELD LFASLVNCFN LPGLPTRHEN VDIVVIRENT 150
    EGEYAGLEHE VVPGVVESLK VITKFCSERI AKYAFEYAYL NNRKKVTAVH 200
    KANIMKLADG LFLESCREVA KKYPSITYNE IIVDNCCMQL VAKPEQFDVM 250
    VTPNLYGNLV ANTAAGIAGG TGVMPGGNVG ADHAVFEQGA SAGNVGKDKI 300
    VLENKANPVA LLLSSAMMLR HLQFPSFADR LETAVKKVIA EGKCRTKDLG 350
    GTSTTQEVVD AVIAKLD 367
    Length:367
    Mass (Da):39,627
    Last modified:January 9, 2007 - v2
    Checksum:i62DF327529961082
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti174 – 1741K → N in AAM61498. 1 PublicationCurated
    Sequence conflicti331 – 3311L → P in AAC49964. (PubMed:9526501)Curated
    Sequence conflicti339 – 3391I → T in AAC49964. (PubMed:9526501)Curated
    Sequence conflicti344 – 3441C → F in AAM61498. 1 PublicationCurated
    Sequence conflicti362 – 3621V → G in AAC49964. (PubMed:9526501)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81993 mRNA. Translation: AAC49964.1.
    U82203 Genomic DNA. Translation: AAC49966.1.
    AL022604 Genomic DNA. Translation: CAA18743.1.
    AL161587 Genomic DNA. Translation: CAB80243.1.
    CP002687 Genomic DNA. Translation: AEE86486.1.
    AF428360 mRNA. Translation: AAL16290.1.
    AY049260 mRNA. Translation: AAK83602.1.
    AY129494 mRNA. Translation: AAM91080.1.
    AY084937 mRNA. Translation: AAM61498.1.
    PIRiT06131.
    RefSeqiNP_195252.1. NM_119692.2.
    UniGeneiAt.22461.
    At.74835.

    Genome annotation databases

    EnsemblPlantsiAT4G35260.1; AT4G35260.1; AT4G35260.
    GeneIDi829679.
    KEGGiath:AT4G35260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81993 mRNA. Translation: AAC49964.1 .
    U82203 Genomic DNA. Translation: AAC49966.1 .
    AL022604 Genomic DNA. Translation: CAA18743.1 .
    AL161587 Genomic DNA. Translation: CAB80243.1 .
    CP002687 Genomic DNA. Translation: AEE86486.1 .
    AF428360 mRNA. Translation: AAL16290.1 .
    AY049260 mRNA. Translation: AAK83602.1 .
    AY129494 mRNA. Translation: AAM91080.1 .
    AY084937 mRNA. Translation: AAM61498.1 .
    PIRi T06131.
    RefSeqi NP_195252.1. NM_119692.2.
    UniGenei At.22461.
    At.74835.

    3D structure databases

    ProteinModelPortali Q8LFC0.
    SMRi Q8LFC0. Positions 40-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 14961. 4 interactions.
    IntActi Q8LFC0. 4 interactions.

    Proteomic databases

    PaxDbi Q8LFC0.
    PRIDEi Q8LFC0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G35260.1 ; AT4G35260.1 ; AT4G35260 .
    GeneIDi 829679.
    KEGGi ath:AT4G35260.

    Organism-specific databases

    GeneFarmi 4364. 439.
    TAIRi AT4G35260.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    InParanoidi Q8LFC0.
    KOi K00030.
    OMAi AMHAPIF.
    PhylomeDBi Q8LFC0.

    Gene expression databases

    Genevestigatori Q8LFC0.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana. Characterization of two closely related subunits."
      Behal R.H., Oliver D.J.
      Plant Mol. Biol. 36:691-698(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-171.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    7. "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana."
      Lin M., Behal R.H., Oliver D.J.
      Plant Sci. 166:983-988(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: GENE FAMILY.
    8. "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs."
      Lemaitre T., Hodges M.
      Plant Cell Physiol. 47:634-643(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIDH1_ARATH
    AccessioniPrimary (citable) accession number: Q8LFC0
    Secondary accession number(s): O65501, P94015, Q7DM90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3