Cyclin-dependent kinase B2-1 - Arabidopsis thaliana (Mouse-ear cress)

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Q8LF80 (CKB21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase B2-1
Short name=CDKB2;1
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	CDKB2-1
Ordered Locus Names:	At1g76540
ORF Names:	F14G6.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Interacts with CYCD4-1 and CKS1. Ref.1 Ref.2
Tissue specificity	Expressed in root tips, shoot apical meristem, leaf primordia vascular tissues and tapetum of anthers. Ref.1 Ref.2 Ref.9
Developmental stage	Expressed from early G2 phase and increases to reach a peak at mitosis. Ref.2
Miscellaneous	Protein abundance may be regulated through proteasome-mediated protein degradation.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAM61558.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Traceable author statement Ref.2. Source: TAIR histone phosphorylation Inferred from direct assay Ref.2. Source: TAIR hormone-mediated signaling pathway Inferred from expression pattern PubMed 18223038. Source: TAIR regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 18223038. Source: TAIR regulation of meristem structural organization Inferred from mutant phenotype PubMed 18223038. Source: TAIR
Cellular_component	cyclin-dependent protein kinase holoenzyme complex Inferred from direct assay Ref.2. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CKS1	O23249	5	EBI-1253579,EBI-1253127
CYCD1-1	P42751	2	EBI-1253579,EBI-2025690
CYCD4-1	Q8LGA1	4	EBI-1253579,EBI-1253202

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Cyclin-dependent kinase B2-1

PRO_0000293115

Regions

Domain

14 – 304

291

Protein kinase

Nucleotide binding

20 – 28

ATP By similarity

Sites

Active site

145

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

179

Phosphothreonine By similarity

Experimental info

Sequence conflict

250

S → G in AAK63856. Ref.5

Sequence conflict

250

S → G in AAN28798. Ref.5

Sequence conflict

267

S → F in AAK63856. Ref.5

Sequence conflict

267

S → F in AAN28798. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q8LF80 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 7E0EBD7A553DB9C9
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FASTA

313

35,593

        10         20         30         40         50         60 
MDEGVIAVSA MDAFEKLEKV GEGTYGKVYR AREKATGKIV ALKKTRLHED EEGVPSTTLR 

        70         80         90        100        110        120 
EISILRMLAR DPHVVRLMDV KQGLSKEGKT VLYLVFEYMD TDVKKFIRSF RSTGKNIPTQ 

       130        140        150        160        170        180 
TIKSLMYQLC KGMAFCHGHG ILHRDLKPHN LLMDPKTMRL KIADLGLARA FTLPMKKYTH 

       190        200        210        220        230        240 
EILTLWYRAP EVLLGATHYS TAVDMWSVGC IFAELVTNQA IFQGDSELQQ LLHIFKLFGT 

       250        260        270        280        290        300 
PNEEMWPGVS TLKNWHEYPQ WKPSTLSSAV PNLDEAGVDL LSKMLQYEPA KRISAKMAME 

       310 
HPYFDDLPEK SSL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis." Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L. J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
[2]	"Arabidopsis D-type cyclin CYCD4;1 is a novel cyclin partner of B2-type cyclin-dependent kinase." Kono A., Umeda-Hara C., Lee J., Ito M., Uchimiya H., Umeda M. Plant Physiol. 132:1315-1321(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CYCD4-1.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana." Adachi S., Uchimiya H., Umeda M. Plant Cell Physiol. 47:1683-1686(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ297936 mRNA. Translation: CAC34052.1. AB047279 mRNA. Translation: BAB62068.1. AC015450 Genomic DNA. Translation: AAG51960.1. CP002684 Genomic DNA. Translation: AEE35857.1. AF389283 mRNA. Translation: AAK63856.1. AY143859 mRNA. Translation: AAN28798.1. AY085000 mRNA. Translation: AAM61558.1. Different initiation.
IPI	IPI00516272.
PIR	D96793.
RefSeq	NP_177780.1. NM_106304.2.
UniGene	At.10322.
3D structure databases
HSSP	HSSP built from PDB template 1OIT based on UniProtKB P24941.
ProteinModelPortal	Q8LF80.
SMR	Q8LF80. Positions 10-307.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LF80. 12 interactions.
Proteomic databases
PaxDb	Q8LF80.
PRIDE	Q8LF80.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G76540.1; AT1G76540.1; AT1G76540.
GeneID	843987.
KEGG	ath:AT1G76540.
Organism-specific databases
GeneFarm	3281. 107.
TAIR	At1g76540.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q8LF80.
KO	K00924.
OMA	ISAKMAM.
PhylomeDB	Q8LF80.
ProtClustDB	CLSN2679448.
Gene expression databases
Genevestigator	Q8LF80.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CKB21_ARATH

Accession

Primary (citable) accession number: Q8LF80
Secondary accession number(s): Q94EX2, Q9C527

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents