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Q8LF80

- CKB21_ARATH

UniProt

Q8LF80 - CKB21_ARATH

Protein

Cyclin-dependent kinase B2-1

Gene

CDKB2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431ATPPROSITE-ProRule annotation
    Active sitei145 – 1451Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 289ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: TAIR
    3. protein binding Source: IntAct
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: TAIR
    2. histone phosphorylation Source: TAIR
    3. hormone-mediated signaling pathway Source: TAIR
    4. regulation of G2/M transition of mitotic cell cycle Source: TAIR
    5. regulation of meristem structural organization Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G76540-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase B2-1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKB2;1
    Gene namesi
    Name:CDKB2-1
    Ordered Locus Names:At1g76540
    ORF Names:F14G6.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G76540.

    Subcellular locationi

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Cyclin-dependent kinase B2-1PRO_0000293115Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei25 – 251PhosphotyrosineBy similarity
    Modified residuei179 – 1791PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8LF80.
    PRIDEiQ8LF80.

    Expressioni

    Tissue specificityi

    Expressed in root tips, shoot apical meristem, leaf primordia vascular tissues and tapetum of anthers.3 Publications

    Developmental stagei

    Expressed from early G2 phase and increases to reach a peak at mitosis.1 Publication

    Gene expression databases

    GenevestigatoriQ8LF80.

    Interactioni

    Subunit structurei

    Interacts with CYCD4-1 and CKS1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CKS1O232495EBI-1253579,EBI-1253127
    CYCD1-1P427512EBI-1253579,EBI-2025690
    CYCD4-1Q8LGA14EBI-1253579,EBI-1253202

    Protein-protein interaction databases

    BioGridi29206. 35 interactions.
    IntActiQ8LF80. 12 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LF80.
    SMRiQ8LF80. Positions 10-307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 304291Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ8LF80.
    KOiK07760.
    OMAiLMDPKTM.
    PhylomeDBiQ8LF80.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8LF80-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEGVIAVSA MDAFEKLEKV GEGTYGKVYR AREKATGKIV ALKKTRLHED    50
    EEGVPSTTLR EISILRMLAR DPHVVRLMDV KQGLSKEGKT VLYLVFEYMD 100
    TDVKKFIRSF RSTGKNIPTQ TIKSLMYQLC KGMAFCHGHG ILHRDLKPHN 150
    LLMDPKTMRL KIADLGLARA FTLPMKKYTH EILTLWYRAP EVLLGATHYS 200
    TAVDMWSVGC IFAELVTNQA IFQGDSELQQ LLHIFKLFGT PNEEMWPGVS 250
    TLKNWHEYPQ WKPSTLSSAV PNLDEAGVDL LSKMLQYEPA KRISAKMAME 300
    HPYFDDLPEK SSL 313
    Length:313
    Mass (Da):35,593
    Last modified:July 10, 2007 - v2
    Checksum:i7E0EBD7A553DB9C9
    GO

    Sequence cautioni

    The sequence AAM61558.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti250 – 2501S → G in AAK63856. (PubMed:14593172)Curated
    Sequence conflicti250 – 2501S → G in AAN28798. (PubMed:14593172)Curated
    Sequence conflicti267 – 2671S → F in AAK63856. (PubMed:14593172)Curated
    Sequence conflicti267 – 2671S → F in AAN28798. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297936 mRNA. Translation: CAC34052.1.
    AB047279 mRNA. Translation: BAB62068.1.
    AC015450 Genomic DNA. Translation: AAG51960.1.
    CP002684 Genomic DNA. Translation: AEE35857.1.
    AF389283 mRNA. Translation: AAK63856.1.
    AY143859 mRNA. Translation: AAN28798.1.
    AY085000 mRNA. Translation: AAM61558.1. Different initiation.
    PIRiD96793.
    RefSeqiNP_177780.1. NM_106304.2.
    UniGeneiAt.10322.

    Genome annotation databases

    EnsemblPlantsiAT1G76540.1; AT1G76540.1; AT1G76540.
    GeneIDi843987.
    KEGGiath:AT1G76540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ297936 mRNA. Translation: CAC34052.1 .
    AB047279 mRNA. Translation: BAB62068.1 .
    AC015450 Genomic DNA. Translation: AAG51960.1 .
    CP002684 Genomic DNA. Translation: AEE35857.1 .
    AF389283 mRNA. Translation: AAK63856.1 .
    AY143859 mRNA. Translation: AAN28798.1 .
    AY085000 mRNA. Translation: AAM61558.1 . Different initiation.
    PIRi D96793.
    RefSeqi NP_177780.1. NM_106304.2.
    UniGenei At.10322.

    3D structure databases

    ProteinModelPortali Q8LF80.
    SMRi Q8LF80. Positions 10-307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 29206. 35 interactions.
    IntActi Q8LF80. 12 interactions.

    Proteomic databases

    PaxDbi Q8LF80.
    PRIDEi Q8LF80.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G76540.1 ; AT1G76540.1 ; AT1G76540 .
    GeneIDi 843987.
    KEGGi ath:AT1G76540.

    Organism-specific databases

    GeneFarmi 3281. 107.
    TAIRi AT1G76540.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q8LF80.
    KOi K07760.
    OMAi LMDPKTM.
    PhylomeDBi Q8LF80.

    Enzyme and pathway databases

    BioCyci ARA:AT1G76540-MONOMER.

    Gene expression databases

    Genevestigatori Q8LF80.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
      Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
      J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
    2. "Arabidopsis D-type cyclin CYCD4;1 is a novel cyclin partner of B2-type cyclin-dependent kinase."
      Kono A., Umeda-Hara C., Lee J., Ito M., Uchimiya H., Umeda M.
      Plant Physiol. 132:1315-1321(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CYCD4-1.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
      Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
      Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Cell cycle regulation in plant development."
      Inze D., de Veylder L.
      Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    9. "Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana."
      Adachi S., Uchimiya H., Umeda M.
      Plant Cell Physiol. 47:1683-1686(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCKB21_ARATH
    AccessioniPrimary (citable) accession number: Q8LF80
    Secondary accession number(s): Q94EX2, Q9C527
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Protein abundance may be regulated through proteasome-mediated protein degradation.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3