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Q8LF80 (CKB21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase B2-1

Short name=CDKB2;1
EC=2.7.11.22
EC=2.7.11.23
Gene names
Name:CDKB2-1
Ordered Locus Names:At1g76540
ORF Names:F14G6.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Interacts with CYCD4-1 and CKS1. Ref.1 Ref.2

Tissue specificity

Expressed in root tips, shoot apical meristem, leaf primordia vascular tissues and tapetum of anthers. Ref.1 Ref.2 Ref.9

Developmental stage

Expressed from early G2 phase and increases to reach a peak at mitosis. Ref.2

Miscellaneous

Protein abundance may be regulated through proteasome-mediated protein degradation.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAM61558.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Cyclin-dependent kinase B2-1
PRO_0000293115

Regions

Domain14 – 304291Protein kinase
Nucleotide binding20 – 289ATP By similarity

Sites

Active site1451Proton acceptor By similarity
Binding site431ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue251Phosphotyrosine By similarity
Modified residue1791Phosphothreonine By similarity

Experimental info

Sequence conflict2501S → G in AAK63856. Ref.5
Sequence conflict2501S → G in AAN28798. Ref.5
Sequence conflict2671S → F in AAK63856. Ref.5
Sequence conflict2671S → F in AAN28798. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q8LF80 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 7E0EBD7A553DB9C9

FASTA31335,593
        10         20         30         40         50         60 
MDEGVIAVSA MDAFEKLEKV GEGTYGKVYR AREKATGKIV ALKKTRLHED EEGVPSTTLR 

        70         80         90        100        110        120 
EISILRMLAR DPHVVRLMDV KQGLSKEGKT VLYLVFEYMD TDVKKFIRSF RSTGKNIPTQ 

       130        140        150        160        170        180 
TIKSLMYQLC KGMAFCHGHG ILHRDLKPHN LLMDPKTMRL KIADLGLARA FTLPMKKYTH 

       190        200        210        220        230        240 
EILTLWYRAP EVLLGATHYS TAVDMWSVGC IFAELVTNQA IFQGDSELQQ LLHIFKLFGT 

       250        260        270        280        290        300 
PNEEMWPGVS TLKNWHEYPQ WKPSTLSSAV PNLDEAGVDL LSKMLQYEPA KRISAKMAME 

       310 
HPYFDDLPEK SSL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of novel cyclin-dependent kinases interacting with the CKS1 protein of Arabidopsis."
Boudolf V., Rombauts S., Naudts M., Inze D., de Veylder L.
J. Exp. Bot. 52:1381-1382(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH CKS1.
[2]"Arabidopsis D-type cyclin CYCD4;1 is a novel cyclin partner of B2-type cyclin-dependent kinase."
Kono A., Umeda-Hara C., Lee J., Ito M., Uchimiya H., Umeda M.
Plant Physiol. 132:1315-1321(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH CYCD4-1.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Genome-wide analysis of core cell cycle genes in Arabidopsis."
Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Cell cycle regulation in plant development."
Inze D., de Veylder L.
Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Expression of B2-type cyclin-dependent kinase is controlled by protein degradation in Arabidopsis thaliana."
Adachi S., Uchimiya H., Umeda M.
Plant Cell Physiol. 47:1683-1686(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ297936 mRNA. Translation: CAC34052.1.
AB047279 mRNA. Translation: BAB62068.1.
AC015450 Genomic DNA. Translation: AAG51960.1.
CP002684 Genomic DNA. Translation: AEE35857.1.
AF389283 mRNA. Translation: AAK63856.1.
AY143859 mRNA. Translation: AAN28798.1.
AY085000 mRNA. Translation: AAM61558.1. Different initiation.
PIRD96793.
RefSeqNP_177780.1. NM_106304.2.
UniGeneAt.10322.

3D structure databases

ProteinModelPortalQ8LF80.
SMRQ8LF80. Positions 10-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid29206. 35 interactions.
IntActQ8LF80. 12 interactions.

Proteomic databases

PaxDbQ8LF80.
PRIDEQ8LF80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G76540.1; AT1G76540.1; AT1G76540.
GeneID843987.
KEGGath:AT1G76540.

Organism-specific databases

GeneFarm3281. 107.
TAIRAT1G76540.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ8LF80.
KOK07760.
OMALMDPKTM.
PhylomeDBQ8LF80.

Enzyme and pathway databases

BioCycARA:AT1G76540-MONOMER.

Gene expression databases

GenevestigatorQ8LF80.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCKB21_ARATH
AccessionPrimary (citable) accession number: Q8LF80
Secondary accession number(s): Q94EX2, Q9C527
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names