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Protein

Serine/threonine protein phosphatase 2A 57 kDa regulatory subunit B' theta isoform

Gene

B'THETA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The B regulatory subunit may modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity). Associates with the serine/threonine-protein phosphatase PP2A catalytic subunit C and regulatory subunit A to positively regulates beta-oxidation of fatty acids and protoauxins in peroxisomes by dephosphorylating peroxisomal beta-oxidation-related proteins (PubMed:25489022). Required for the formation of the PP2A holoenzyme that negatively regulates brassinosteroid signaling by dephosphorylating and inactivating BRI1 in the cytoplasm (PubMed:26517938).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid mediated signaling pathway Source: UniProtKB-KW
  • negative regulation of brassinosteroid mediated signaling pathway Source: UniProtKB
  • positive regulation of fatty acid beta-oxidation Source: UniProtKB

Keywordsi

Biological processBrassinosteroid signaling pathway

Enzyme and pathway databases

ReactomeiR-ATH-198753. ERK/MAPK targets.
R-ATH-202670. ERKs are inactivated.
R-ATH-5673000. RAF activation.
R-ATH-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine protein phosphatase 2A 57 kDa regulatory subunit B' theta isoform
Short name:
AtB' theta
Short name:
PP2A, B' subunit, theta isoform
Gene namesi
Name:B'THETA
Ordered Locus Names:At1g13460
ORF Names:T6J4.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G13460.
TAIRilocus:2009912. AT1G13460.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Peroxisome

Pathology & Biotechi

Disruption phenotypei

Reduced plant size and fresh weight.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi489 – 492Missing : Loss of peroxisomal targeting. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714671 – 492Serine/threonine protein phosphatase 2A 57 kDa regulatory subunit B' theta isoformAdd BLAST492

Proteomic databases

PaxDbiQ8LF36.
PRIDEiQ8LF36.

Expressioni

Tissue specificityi

Highly expressed in dry seeds. Expressed in roots, cotyledons, rosette leaves and flowers.1 Publication

Inductioni

Induced by epibrassinolide.1 Publication

Gene expression databases

ExpressionAtlasiQ8LF36. baseline and differential.
GenevisibleiQ8LF36. AT.

Interactioni

Subunit structurei

PP2A consists of a common heteromeric enzyme, composed of a catalytic subunit (subunits C), a constant regulatory subunit (subunit A), and a variety of regulatory subunits such as subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) (By similarity). Interacts with BZR1 (PubMed:21258370). Interacts with PP2A2, PP2A5 and PP2AA2 (PubMed:25489022).By similarity2 Publications

Protein-protein interaction databases

BioGridi23146. 2 interactors.
STRINGi3702.AT1G13460.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LF36.
SMRiQ8LF36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi490 – 492Microbody targeting signal1 Publication3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 39Ser-richAdd BLAST27

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2085. Eukaryota.
ENOG410XQJW. LUCA.
HOGENOMiHOG000067326.
KOiK11584.
OMAiKFREDES.
OrthoDBiEOG0936077A.
PhylomeDBiQ8LF36.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002554. PP2A_B56.
PANTHERiPTHR10257. PTHR10257. 1 hit.
PfamiView protein in Pfam
PF01603. B56. 1 hit.
PIRSFiPIRSF028043. PP2A_B56. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8LF36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKQILSKLP KKSSSKNHSS SSSSTSKSSD NGASKSGNSQ TQNAPPVKPS
60 70 80 90 100
ADSGFKEGNL KGNGNGFTPY EALPGFKDVP NAEKQNLFVR KLSLCCVVFD
110 120 130 140 150
FSDPTKNVKE KDIKRQTLLE LVDYVASPNG KFSETVIQEV VRMVSVNIFR
160 170 180 190 200
TLNPQPRENK VIDALDLEEE EPSMDPTWPH LQLVYEILLR LIASPETDTK
210 220 230 240 250
LAKKYIDQSF VSRLLDLFDS EDPRERDCLK TVLHRIYGKF MVHRPFIRKS
260 270 280 290 300
INNIFYRFVF ETEKHNGIAE FLEILGSIIN GFALPLKDEH KVFLVRALVP
310 320 330 340 350
LHKPKSLQMY HQQLSYCITQ FVEKDCKLAD TVIRGLLKSW PVTNSSKEVM
360 370 380 390 400
FLNELEEVLE ATQPPEFQRC MVPLFRQVAR CLNSLHFQVA ERALFLWNND
410 420 430 440 450
HIENLIMQNR KVILPIIFPA LERNTQKHWN QAVHSLTLNV QKIFNDIDAE
460 470 480 490
LFKDCLAKFR EDESKEAEIG AKREATWKRL EEIGNQKQKS SL
Length:492
Mass (Da):56,510
Last modified:January 24, 2006 - v2
Checksum:iE49174DE756FE2CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34S → G in AAM61625 (Ref. 4) Curated1
Sequence conflicti42Q → K in AAM61625 (Ref. 4) Curated1
Sequence conflicti488Q → K in AAM61625 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011810 Genomic DNA. Translation: AAG09562.1.
CP002684 Genomic DNA. Translation: AEE29021.1.
CP002684 Genomic DNA. Translation: AEE29022.1.
AY085069 mRNA. Translation: AAM61625.1.
BT026384 mRNA. Translation: ABH04491.1.
RefSeqiNP_172803.1. NM_101216.2.
NP_973816.1. NM_202087.2.
UniGeneiAt.42029.

Genome annotation databases

EnsemblPlantsiAT1G13460.1; AT1G13460.1; AT1G13460.
AT1G13460.2; AT1G13460.2; AT1G13460.
GeneIDi837906.
GrameneiAT1G13460.1; AT1G13460.1; AT1G13460.
AT1G13460.2; AT1G13460.2; AT1G13460.
KEGGiath:AT1G13460.

Similar proteinsi

Entry informationi

Entry namei2A5T_ARATH
AccessioniPrimary (citable) accession number: Q8LF36
Secondary accession number(s): Q0V836, Q9FX52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: August 30, 2017
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families