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Protein

Putative clathrin assembly protein At2g25430

Gene

At2g25430

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Putative clathrin assembly protein At2g25430
Gene namesi
Ordered Locus Names:At2g25430
ORF Names:F13B15.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G25430.

Subcellular locationi

GO - Cellular componenti

  • clathrin-coated pit Source: UniProtKB-SubCell
  • clathrin-coated vesicle Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001870681 – 653Putative clathrin assembly protein At2g25430Add BLAST653

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei221PhosphoserineBy similarity1
Modified residuei244PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8LF20.
PRIDEiQ8LF20.

PTM databases

iPTMnetiQ8LF20.

Expressioni

Gene expression databases

GenevisibleiQ8LF20. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G25430.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LF20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 159ENTHPROSITE-ProRule annotationAdd BLAST137

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi198 – 236Gly-richAdd BLAST39
Compositional biasi391 – 431Pro-richAdd BLAST41

Sequence similaritiesi

Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000238534.
InParanoidiQ8LF20.
OMAiFYNWCKE.
OrthoDBiEOG09360683.
PhylomeDBiQ8LF20.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF07651. ANTH. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8LF20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSIRKAIG AVKDQTSIGI AKVASNMAPD LEVAIVKATS HDDDPASEKY
60 70 80 90 100
IREILNLTSL SRGYILACVT SVSRRLSKTR DWVVALKALM LVHRLLNEGD
110 120 130 140 150
PIFQEEILYS TRRGTRMLNM SDFRDEAHSS SWDHSAFVRT YAGYLDQRLE
160 170 180 190 200
LALFERKSGV SVNSGGNSSH HSNNDDRYGR GRDDFRSPPP RSYDYENGGG
210 220 230 240 250
GGSDFRGDNN GYGGVPKRSR SYGDMTEMGG GGGGGGRDEK KVVTPLREMT
260 270 280 290 300
PERIFGKMGH LQRLLDRFLS LRPTGLAKNS RMILIALYPV VRESFKLYAD
310 320 330 340 350
ICEVLAVLLD KFFDMEYSDC VKAFDAYASA AKQIDELIAF YNWCKETGVA
360 370 380 390 400
RSSEYPEVQR ITSKLLETLE EFVRDRAKRG KSPERKEIEA PPPVVEEEEP
410 420 430 440 450
EPDMNEIKAL PPPENYTPPP PPEPEPQPEK PQFTEDLVNL REDEVTADDQ
460 470 480 490 500
GNKFALALFA GPPGNNGKWE AFSSNGVTSA WQNPAAEPGK ADWELALVET
510 520 530 540 550
TSNLEKQTAA LGGGFDNLLL NGMYDQGMVR QHVSTSQLTG GSASSVALPL
560 570 580 590 600
PGKTNNQVLA LPAPDGTVEK VNQDPFAASL TIPPPSYVQM AEMEKKQYLL
610 620 630 640 650
SQEQQLWQQY QRDGMRGQAS LAKMNTGPVP AYGMPPVNGM GPPPTGYYYN

NPY
Length:653
Mass (Da):72,084
Last modified:April 13, 2004 - v2
Checksum:iA61B21EBA98206D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203Missing in AAM61646 (Ref. 4) Curated1
Sequence conflicti236G → GG in AAM61646 (Ref. 4) Curated1
Sequence conflicti501T → A in AAM61646 (Ref. 4) Curated1
Sequence conflicti631A → T in AAM61646 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006300 Genomic DNA. Translation: AAD20703.1.
CP002685 Genomic DNA. Translation: AEC07701.1.
AY139760 mRNA. Translation: AAM98081.1.
BT008299 mRNA. Translation: AAP37658.1.
AY085092 mRNA. Translation: AAM61646.1.
PIRiC84648.
RefSeqiNP_565595.1. NM_128100.4.
UniGeneiAt.20747.

Genome annotation databases

EnsemblPlantsiAT2G25430.1; AT2G25430.1; AT2G25430.
GeneIDi817081.
GrameneiAT2G25430.1; AT2G25430.1; AT2G25430.
KEGGiath:AT2G25430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006300 Genomic DNA. Translation: AAD20703.1.
CP002685 Genomic DNA. Translation: AEC07701.1.
AY139760 mRNA. Translation: AAM98081.1.
BT008299 mRNA. Translation: AAP37658.1.
AY085092 mRNA. Translation: AAM61646.1.
PIRiC84648.
RefSeqiNP_565595.1. NM_128100.4.
UniGeneiAt.20747.

3D structure databases

ProteinModelPortaliQ8LF20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G25430.1.

PTM databases

iPTMnetiQ8LF20.

Proteomic databases

PaxDbiQ8LF20.
PRIDEiQ8LF20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G25430.1; AT2G25430.1; AT2G25430.
GeneIDi817081.
GrameneiAT2G25430.1; AT2G25430.1; AT2G25430.
KEGGiath:AT2G25430.

Organism-specific databases

TAIRiAT2G25430.

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000238534.
InParanoidiQ8LF20.
OMAiFYNWCKE.
OrthoDBiEOG09360683.
PhylomeDBiQ8LF20.

Miscellaneous databases

PROiQ8LF20.

Gene expression databases

GenevisibleiQ8LF20. AT.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF07651. ANTH. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAP2_ARATH
AccessioniPrimary (citable) accession number: Q8LF20
Secondary accession number(s): Q9SKK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.