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Protein

Calcineurin B-like protein 3

Gene

CBL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binds calcium ions. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Negatively regulates the enzyme activity of MTN1 in the presence of calcium.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei155Involved in dimerizationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi58 – 711; atypicalAdd BLAST14
Calcium bindingi95 – 1062PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi132 – 1433PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi176 – 1874PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: TAIR

GO - Biological processi

  • detection of calcium ion Source: TAIR
  • potassium ion homeostasis Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin B-like protein 3
Alternative name(s):
SOS3-like calcium-binding protein 6
Gene namesi
Name:CBL3
Synonyms:SCABP6
Ordered Locus Names:At4g26570
ORF Names:T15N24.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26570.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: TAIR
  • plant-type vacuole membrane Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions; due to partial redundancy with CLB2. Tolerant to low-K+ stress. Clb2 and cbl3 double mutants show stunted growth, reduced fertility and necrotic lesions at leaf tips. They also have a reduced vacuolar H+-ATPase activity, are hypersensitive to excessive metal ions and are more tolerant to low-K+ conditions.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000735041 – 226Calcineurin B-like protein 3Add BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216PhosphoserineBy similarity1

Post-translational modificationi

S-acylated by PAT10.

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8LEM7.

PTM databases

iPTMnetiQ8LEM7.
SwissPalmiQ8LEM7.

Expressioni

Tissue specificityi

Ubiquitous. Stronger expression in roots. Expressed in root tip and root hair zone, leaf veins, vascular bundles and vasculature of sepals.3 Publications

Developmental stagei

Expressed early during germination and increases to a peak level when seedlings are established.1 Publication

Gene expression databases

GenevisibleiQ8LEM7. AT.

Interactioni

Subunit structurei

Homodimer (By similarity). Part of a K+-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K+-channel (AKT1). Interacts with PP2CA, CIPK1, CIPK2, CIPK3, CIPK4, CIPK6, CIPK7, CIPK11, CIPK12, CIPK13, CIPK14, CIPK16, CIPK23, and MTN1.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIPK1Q8RWC94EBI-637358,EBI-1748677
CIPK6O655544EBI-637358,EBI-537615

Protein-protein interaction databases

BioGridi14051. 18 interactors.
DIPiDIP-33307N.
IntActiQ8LEM7. 16 interactors.
STRINGi3702.AT4G26570.2.

Structurei

3D structure databases

ProteinModelPortaliQ8LEM7.
SMRiQ8LEM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 81EF-hand 1PROSITE-ProRule annotationAdd BLAST46
Domaini82 – 117EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini119 – 154EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini163 – 198EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Domaini

The N-terminal 22 amino acids are sufficient for vacuolar membrane targeting. The internal domain (109-199) is sufficient for the interaction with MTN1.2 Publications

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
InParanoidiQ8LEM7.
KOiK06268.
OMAiFAKMDKD.
PhylomeDBiQ8LEM7.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8LEM7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQCIDGFKH VCSSFFRCFD IDIYKQSGGL GDPELLARET VFSVSEIEAL
60 70 80 90 100
YELFKKISSA VIDDGLINKE EFQLALFKTN KKESLFADRV FDLFDTKHNG
110 120 130 140 150
ILGFEEFARA LSVFHPNAPI EDKIDFSFQL YDLKQQGFIE RQEVKQMVVA
160 170 180 190 200
TLAESGMNLS DEIIESIIDK TFEEADTKHD GRIDKEEWRT LVLRHPSLLK
210 220
NMTLQYLKDI TTTFPSFVFH SQVEDT
Length:226
Mass (Da):26,032
Last modified:December 21, 2004 - v2
Checksum:iF78222E98F64983E
GO
Isoform 2 (identifier: Q8LEM7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-89: R → RYQSQ

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:230
Mass (Da):26,538
Checksum:i04E7E8CAEC7E5049
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01232889R → RYQSQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076253 mRNA. Translation: AAC26010.1.
AL078465 Genomic DNA. Translation: CAB43853.1.
AL161565 Genomic DNA. Translation: CAB79512.1.
CP002687 Genomic DNA. Translation: AEE85220.1.
CP002687 Genomic DNA. Translation: AEE85221.1.
AY072441 mRNA. Translation: AAL62433.1.
AY128880 mRNA. Translation: AAM91280.1.
AY085344 mRNA. Translation: AAM62575.1.
PIRiT08923.
RefSeqiNP_194387.1. NM_118791.4. [Q8LEM7-1]
UniGeneiAt.22453.

Genome annotation databases

EnsemblPlantsiAT4G26570.1; AT4G26570.1; AT4G26570. [Q8LEM7-1]
GeneIDi828764.
GrameneiAT4G26570.1; AT4G26570.1; AT4G26570.
KEGGiath:AT4G26570.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076253 mRNA. Translation: AAC26010.1.
AL078465 Genomic DNA. Translation: CAB43853.1.
AL161565 Genomic DNA. Translation: CAB79512.1.
CP002687 Genomic DNA. Translation: AEE85220.1.
CP002687 Genomic DNA. Translation: AEE85221.1.
AY072441 mRNA. Translation: AAL62433.1.
AY128880 mRNA. Translation: AAM91280.1.
AY085344 mRNA. Translation: AAM62575.1.
PIRiT08923.
RefSeqiNP_194387.1. NM_118791.4. [Q8LEM7-1]
UniGeneiAt.22453.

3D structure databases

ProteinModelPortaliQ8LEM7.
SMRiQ8LEM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14051. 18 interactors.
DIPiDIP-33307N.
IntActiQ8LEM7. 16 interactors.
STRINGi3702.AT4G26570.2.

PTM databases

iPTMnetiQ8LEM7.
SwissPalmiQ8LEM7.

Proteomic databases

PaxDbiQ8LEM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26570.1; AT4G26570.1; AT4G26570. [Q8LEM7-1]
GeneIDi828764.
GrameneiAT4G26570.1; AT4G26570.1; AT4G26570.
KEGGiath:AT4G26570.

Organism-specific databases

TAIRiAT4G26570.

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
InParanoidiQ8LEM7.
KOiK06268.
OMAiFAKMDKD.
PhylomeDBiQ8LEM7.

Miscellaneous databases

PROiQ8LEM7.

Gene expression databases

GenevisibleiQ8LEM7. AT.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNBL3_ARATH
AccessioniPrimary (citable) accession number: Q8LEM7
Secondary accession number(s): O81447
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.