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Protein

Autophagy-related protein 8a

Gene

ATG8A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. May mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 8a
Alternative name(s):
Autophagy-related ubiquitin-like modifier ATG8a
Short name:
AtAPG8a
Short name:
Protein autophagy 8a
Gene namesi
Name:ATG8A
Synonyms:APG8A
Ordered Locus Names:At4g21980
ORF Names:F1N20.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21980.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 117117Autophagy-related protein 8aPRO_0000286905Add
BLAST
Propeptidei118 – 1225Removed in mature formBy similarityPRO_0000286906

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi117 – 1171Phosphatidylethanolamine amidated glycineBy similarity

Post-translational modificationi

The C-terminal 5 residues are removed by ATG4 to expose Gly-117 at the C-terminus. This Gly-117 forms then a thioester bond with the 'Cys-558' of ATG7 (E1-like activating enzyme) before being transferred to the 'Cys-258' of ATG3 (the specific E2 conjugating enzyme), in order to be finally amidated with phosphatidylethanolamine. This lipid modification anchors ATG8 to autophagosomes (By similarity).By similarity

Keywords - PTMi

Lipoprotein

Proteomic databases

PaxDbiQ8LEM4.

Expressioni

Tissue specificityi

Contitutively expressed.1 Publication

Inductioni

Induced by sugar starvation.1 Publication

Gene expression databases

ExpressionAtlasiQ8LEM4. baseline and differential.
GenevisibleiQ8LEM4. AT.

Interactioni

Subunit structurei

Interacts with ATG4B (PubMed:15178341). Interacts with NBR1 (PubMed:21606687).2 Publications

Protein-protein interaction databases

BioGridi13576. 3 interactions.
STRINGi3702.AT4G21980.2.

Structurei

3D structure databases

ProteinModelPortaliQ8LEM4.
SMRiQ8LEM4. Positions 5-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
HOGENOMiHOG000232034.
InParanoidiQ8LEM4.
KOiK08341.
PhylomeDBiQ8LEM4.

Family and domain databases

InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8LEM4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKSSFKISN PLEARMSESS RIREKYPDRI PVIVEKAGQS DVPDIDKKKY
60 70 80 90 100
LVPADLTVGQ FVYVVRKRIK LGAEKAIFVF VKNTLPPTAA LMSAIYEEHK
110 120
DEDGFLYMTY SGENTFGSLT VA
Length:122
Mass (Da):13,695
Last modified:May 15, 2007 - v2
Checksum:i9F04845BD559349B
GO

Sequence cautioni

The sequence CAA18101.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB79153.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492759 mRNA. Translation: AAM70188.1.
AB073175 mRNA. Translation: BAB88387.1.
AL022140 Genomic DNA. Translation: CAA18101.1. Sequence problems.
AL161556 Genomic DNA. Translation: CAB79153.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84537.1.
BT002024 mRNA. Translation: AAN72035.1.
BT006522 mRNA. Translation: AAP21330.1.
AY085349 mRNA. Translation: AAM62580.1.
PIRiT49105.
RefSeqiNP_567642.1. NM_118319.3. [Q8LEM4-1]
UniGeneiAt.2106.
At.24034.

Genome annotation databases

EnsemblPlantsiAT4G21980.1; AT4G21980.1; AT4G21980. [Q8LEM4-1]
GeneIDi828287.
KEGGiath:AT4G21980.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492759 mRNA. Translation: AAM70188.1.
AB073175 mRNA. Translation: BAB88387.1.
AL022140 Genomic DNA. Translation: CAA18101.1. Sequence problems.
AL161556 Genomic DNA. Translation: CAB79153.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84537.1.
BT002024 mRNA. Translation: AAN72035.1.
BT006522 mRNA. Translation: AAP21330.1.
AY085349 mRNA. Translation: AAM62580.1.
PIRiT49105.
RefSeqiNP_567642.1. NM_118319.3. [Q8LEM4-1]
UniGeneiAt.2106.
At.24034.

3D structure databases

ProteinModelPortaliQ8LEM4.
SMRiQ8LEM4. Positions 5-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13576. 3 interactions.
STRINGi3702.AT4G21980.2.

Proteomic databases

PaxDbiQ8LEM4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21980.1; AT4G21980.1; AT4G21980. [Q8LEM4-1]
GeneIDi828287.
KEGGiath:AT4G21980.

Organism-specific databases

TAIRiAT4G21980.

Phylogenomic databases

eggNOGiKOG1654. Eukaryota.
ENOG4111JAT. LUCA.
HOGENOMiHOG000232034.
InParanoidiQ8LEM4.
KOiK08341.
PhylomeDBiQ8LEM4.

Miscellaneous databases

PROiQ8LEM4.

Gene expression databases

ExpressionAtlasiQ8LEM4. baseline and differential.
GenevisibleiQ8LEM4. AT.

Family and domain databases

InterProiIPR004241. Atg8-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana."
    Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.
    J. Biol. Chem. 277:33105-33114(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene."
    Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D., Tabata S., Ohsumi Y.
    Plant Physiol. 129:1181-1193(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, GENE FAMILY.
  3. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Arabidopsis homologues of the autophagy protein Atg8 are a novel family of microtubule binding proteins."
    Ketelaar T., Voss C., Dimmock S.A., Thumm M., Hussey P.J.
    FEBS Lett. 567:302-306(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG4B, BINDING TO MICROTUBULES.
  8. "Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy."
    Yoshimoto K., Hanaoka H., Sato S., Kato T., Tabata S., Noda T., Ohsumi Y.
    Plant Cell 16:2967-2983(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "The autophagy-associated Atg8 gene family operates both under favourable growth conditions and under starvation stresses in Arabidopsis plants."
    Slavikova S., Shy G., Yao Y., Glozman R., Levanony H., Pietrokovski S., Elazar Z., Galili G.
    J. Exp. Bot. 56:2839-2849(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1."
    Svenning S., Lamark T., Krause K., Johansen T.
    Autophagy 7:993-1010(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NBR1.

Entry informationi

Entry nameiATG8A_ARATH
AccessioniPrimary (citable) accession number: Q8LEM4
Secondary accession number(s): O65447, Q8S928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: July 6, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.