Q8LEJ9 (B3GT4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 4
EC=2.4.1.-

Gene names

Name:	B3GALT4
Ordered Locus Names:	At4g26940
ORF Names:	F10M23.280

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence CAB36540.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79549.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRL1	Q9ZPS9	1	EBI-2293968,EBI-2292728

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8LEJ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8LEJ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     282-306: GVRYHEPEYWKFGEEGNKYFRHATG → SALCNIQGVGVLHIDKPKRTSQICE
     307-407: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Probable beta-1,3-galactosyltransferase 4		PRO_0000359414
Regions
	Transmembrane	23 – 39	17	Helical; Signal-anchor for type II membrane protein; Potential
Natural variations
	Alternative sequence	282 – 306	25	GVRYH…RHATG → SALCNIQGVGVLHIDKPKRT SQICE in isoform 2.		VSP_036144
	Alternative sequence	307 – 407	101	Missing in isoform 2.		VSP_036145

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 756C0F8866E1C952	FASTA	407	46,020
	10 20 30 40 50 60 MSLKHHHRGL ELSASKSFVS KKWTLFLCIG FFCAGILFSD RMWPEPESNV VSRDTVASDE 70 80 90 100 110 120 RLRLESEDCD SSKKGLKRES KDILGDVYKS PDAIQTLDKT ISKLETELAD ARAAQESIMN 130 140 150 160 170 180 GSPVSDDFKL PETVTKRKYL MVVGVNTAFS SRKRRDSVRA TWMPPGEERK KLEEEKGIVM 190 200 210 220 230 240 RFVIGHSSTP GGILDRAIQA EESKHGDFLR LDHVEGYLEL SAKTKTYFTT AFAMWDADFY 250 260 270 280 290 300 VKVDDDVHVN IATLGAELAR YRMKPRVYIG CMKSGPVLAQ KGVRYHEPEY WKFGEEGNKY 310 320 330 340 350 360 FRHATGQLYA ISRELASYIS INQNVLHKYV NEDVSLGSWF LGLDVEHVDD RRLCCGTTDC 370 380 390 400 EWKAQAGNIC VASFDWSCSG ICRSADRMKD VHRRCGEGEK ALLAASF
	Isoform 2 [UniParc]. Checksum: 2C74E07F9B3C42CE	FASTA	306	34,385
	10 20 30 40 50 60 MSLKHHHRGL ELSASKSFVS KKWTLFLCIG FFCAGILFSD RMWPEPESNV VSRDTVASDE 70 80 90 100 110 120 RLRLESEDCD SSKKGLKRES KDILGDVYKS PDAIQTLDKT ISKLETELAD ARAAQESIMN 130 140 150 160 170 180 GSPVSDDFKL PETVTKRKYL MVVGVNTAFS SRKRRDSVRA TWMPPGEERK KLEEEKGIVM 190 200 210 220 230 240 RFVIGHSSTP GGILDRAIQA EESKHGDFLR LDHVEGYLEL SAKTKTYFTT AFAMWDADFY 250 260 270 280 290 300 VKVDDDVHVN IATLGAELAR YRMKPRVYIG CMKSGPVLAQ KSALCNIQGV GVLHIDKPKR TSQICE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL035440 Genomic DNA. Translation: CAB36540.1. Sequence problems. AL161566 Genomic DNA. Translation: CAB79549.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE85272.1. CP002687 Genomic DNA. Translation: AEE85273.1. AY085383 mRNA. Translation: AAM62612.1.
IPI	IPI00531374. IPI00536564.
PIR	T04817.
RefSeq	NP_567762.1. NM_118828.3. NP_849454.1. NM_179123.3.
UniGene	At.22123.
3D structure databases
ProteinModelPortal	Q8LEJ9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LEJ9. 1 interaction.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q8LEJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G26940.1; AT4G26940.1; AT4G26940.
GeneID	828801.
GenomeReviews	Gene locus AT4G26940 in contig CT486007_GR.
KEGG	ath:AT4G26940.
NMPDR	fig|3702.1.peg.20625.
Organism-specific databases
TAIR	At4g26940.
Phylogenomic databases
eggNOG	KOG2288.
GeneTree	EPGT00070000028122.
HOGENOM	HBG318611.
InParanoid	Q8LEJ9.
OMA	LFSDRMW.
PhylomeDB	Q8LEJ9.
ProtClustDB	CLSN2917617.
Gene expression databases
Genevestigator	Q8LEJ9.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GT4_ARATH

Accession

Primary (citable) accession number: Q8LEJ9
Secondary accession number(s): Q3E9W1, Q9SZ33

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program