Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8LEE8

- LISC_ARATH

UniProt

Q8LEE8 - LISC_ARATH

Protein

Lipoyl synthase, chloroplastic

Gene

LIP1P

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.1 PublicationUniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi127 – 1271Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi132 – 1321Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi165 – 1651Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, chloroplasticUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoate synthase, plastidialUniRule annotation
    Short name:
    LIP1pUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIP1PUniRule annotation
    Ordered Locus Names:At5g08415
    ORF Names:F8L15.140
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G08415.

    Subcellular locationi

    Plastidchloroplast 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636ChloroplastUniRule annotationAdd
    BLAST
    Chaini37 – 394358Lipoyl synthase, chloroplasticPRO_0000398858Add
    BLAST

    Proteomic databases

    PaxDbiQ8LEE8.
    PRIDEiQ8LEE8.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves and flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ8LEE8.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G08415.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LEE8.
    SMRiQ8LEE8. Positions 152-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    InParanoidiQ8LSZ8.
    KOiK03644.
    OMAiTIRAVRH.
    PhylomeDBiQ8LEE8.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LEE8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMHHCSITKP TFSISISTQK LHHHSSKFLN LGFRIRCESG DVSSPLRTKA    50
    VSLSSEMEDS SSLKKSLMEL EGKKSEPYPG GMPKMGPFTG RDPNVKKPAW 100
    LRQKAPQGER FQEVKESLSR LNLNTVCEEA QCPNIGECWN GGGDGVATAT 150
    IMVLGDTCTR GCRFCAVKTS RNPPPPDPME PENTAKAIAS WGVDYIVITS 200
    VDRDDIPDGG SGHFAQTVKA MKRHKPDIMI ECLTSDFRGD LEAVDTLVHS 250
    GLDVFAHNVE TVKRLQRLVR DPRAGYEQSM SVLKHAKISK PGMITKTSIM 300
    LGLGETDEEL KEAMADLRAI DVDILTLGQY LQPTPLHLTV KEYVTPEKFD 350
    FWKTYGESIG FRYVASGPLV RSSYRAGELF VKTMVKESYS KSLS 394
    Length:394
    Mass (Da):43,622
    Last modified:October 5, 2010 - v2
    Checksum:i3F1B081CF0216255
    GO

    Sequence cautioni

    The sequence AAM62684.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence CAC08341.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073745 mRNA. Translation: BAB91180.1.
    AL392174 Genomic DNA. Translation: CAC08341.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED91298.1.
    AY085458 mRNA. Translation: AAM62684.1. Different initiation.
    BT025549 mRNA. Translation: ABF58967.1.
    RefSeqiNP_568196.1. NM_120926.2.
    UniGeneiAt.32581.

    Genome annotation databases

    EnsemblPlantsiAT5G08415.1; AT5G08415.1; AT5G08415.
    GeneIDi830740.
    KEGGiath:AT5G08415.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073745 mRNA. Translation: BAB91180.1 .
    AL392174 Genomic DNA. Translation: CAC08341.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED91298.1 .
    AY085458 mRNA. Translation: AAM62684.1 . Different initiation.
    BT025549 mRNA. Translation: ABF58967.1 .
    RefSeqi NP_568196.1. NM_120926.2.
    UniGenei At.32581.

    3D structure databases

    ProteinModelPortali Q8LEE8.
    SMRi Q8LEE8. Positions 152-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G08415.1-P.

    Proteomic databases

    PaxDbi Q8LEE8.
    PRIDEi Q8LEE8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G08415.1 ; AT5G08415.1 ; AT5G08415 .
    GeneIDi 830740.
    KEGGi ath:AT5G08415.

    Organism-specific databases

    TAIRi AT5G08415.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    InParanoidi Q8LSZ8.
    KOi K03644.
    OMAi TIRAVRH.
    PhylomeDBi Q8LEE8.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Gene expression databases

    Genevestigatori Q8LEE8.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in Arabidopsis thaliana."
      Yasuno R., Wada H.
      FEBS Lett. 517:110-114(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiLISC_ARATH
    AccessioniPrimary (citable) accession number: Q8LEE8
    Secondary accession number(s): Q8LSZ8, Q9FT94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 72 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3