Q8LEE8 (LISC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase, chloroplastic
EC=2.8.1.8

Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoate synthase, plastidial
Short name=LIP1p
Lipoic acid synthase

Gene names

Name:	LIP1P
Ordered Locus Names:	At5g08415
ORF Names:	F8L15.140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. Ref.1
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03129
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03129
Subcellular location	Plastid › chloroplast Ref.1.
Tissue specificity	Expressed in roots, leaves and flowers. Ref.1
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.
Sequence caution	The sequence AAM62684.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAC08341.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW lipoate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Chloroplast Potential
	Chain	37 – 394	358	Lipoyl synthase, chloroplastic HAMAP-Rule MF_03129		PRO_0000398858
Sites
	Metal binding	127	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	132	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	138	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	158	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	162	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	165	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8LEE8 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 3F1B081CF0216255	FASTA	394	43,622
	10 20 30 40 50 60 MMHHCSITKP TFSISISTQK LHHHSSKFLN LGFRIRCESG DVSSPLRTKA VSLSSEMEDS 70 80 90 100 110 120 SSLKKSLMEL EGKKSEPYPG GMPKMGPFTG RDPNVKKPAW LRQKAPQGER FQEVKESLSR 130 140 150 160 170 180 LNLNTVCEEA QCPNIGECWN GGGDGVATAT IMVLGDTCTR GCRFCAVKTS RNPPPPDPME 190 200 210 220 230 240 PENTAKAIAS WGVDYIVITS VDRDDIPDGG SGHFAQTVKA MKRHKPDIMI ECLTSDFRGD 250 260 270 280 290 300 LEAVDTLVHS GLDVFAHNVE TVKRLQRLVR DPRAGYEQSM SVLKHAKISK PGMITKTSIM 310 320 330 340 350 360 LGLGETDEEL KEAMADLRAI DVDILTLGQY LQPTPLHLTV KEYVTPEKFD FWKTYGESIG 370 380 390 FRYVASGPLV RSSYRAGELF VKTMVKESYS KSLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in Arabidopsis thaliana." Yasuno R., Wada H. FEBS Lett. 517:110-114(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073745 mRNA. Translation: BAB91180.1. AL392174 Genomic DNA. Translation: CAC08341.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91298.1. AY085458 mRNA. Translation: AAM62684.1. Different initiation. BT025549 mRNA. Translation: ABF58967.1.
IPI	IPI00543656.
RefSeq	NP_568196.1. NM_120926.2.
UniGene	At.32581.
3D structure databases
ProteinModelPortal	Q8LEE8.
SMR	Q8LEE8. Positions 151-351.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G08415.1-P.
Proteomic databases
PaxDb	Q8LEE8.
PRIDE	Q8LEE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G08415.1; AT5G08415.1; AT5G08415.
GeneID	830740.
KEGG	ath:AT5G08415.
Organism-specific databases
TAIR	At5g08415.
Phylogenomic databases
eggNOG	COG0320.
HOGENOM	HOG000235998.
InParanoid	Q8LSZ8.
KO	K03644.
OMA	PSAHHLP.
PhylomeDB	Q8LEE8.
ProtClustDB	PLN02428.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Gene expression databases
Genevestigator	Q8LEE8.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_03129. Lipoyl_synth_plantC.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LISC_ARATH

Accession

Primary (citable) accession number: Q8LEE8
Secondary accession number(s): Q8LSZ8, Q9FT94

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program