Q8LEA8 (EID1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytochrome A-associated F-box protein

Alternative name(s):
Empfindlicher im dunkelroten Licht protein 1

Gene names

Name:	EID1
Ordered Locus Names:	At4g02440
ORF Names:	T14P8.22

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Negative regulator of the phyA signaling pathway that shifts the responsiveness of the phyA signaling system associated with hypocotyl elongation from red to far-red wavelength. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Probable component of an E3 ubiquitin ligase SCF complex. Interacts with SKP1A/ASK1 and SKP1B/ASK2. Ref.7 Ref.8
Subcellular location	Nucleus Ref.7.
Domain	The F-box domain is required for the interaction with SKP1A and SKP1B. Ref.7
Miscellaneous	'Empfindlicher im dunkelroten licht' means sensitive to far-red light in German.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leaf development Inferred from mutant phenotype. Source: TAIR photoperiodism, flowering Inferred from mutant phenotype. Source: TAIR red, far-red light phototransduction Inferred from mutant phenotype Ref.6. Source: TAIR regulation of photomorphogenesis Inferred from mutant phenotype. Source: TAIR
Cellular component	nucleus Inferred from direct assay. Source: TAIR
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASK11	O49484	3	EBI-687388,EBI-401185
ASK14	O81057	3	EBI-687388,EBI-401221
ASK4	Q9LNT9	4	EBI-687388,EBI-604085
SKP1A	Q39255	4	EBI-687388,EBI-532357
SKP1B	Q9FHW7	4	EBI-687388,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Phytochrome A-associated F-box protein		PRO_0000119961
Regions
	Domain	3 – 55	53	F-box
	Motif	197 – 201	5	Nuclear localization signal Ref.7
Experimental info
	Mutagenesis	10	1	P → A: Disrupts interaction with SKP1A and SKP1B. Ref.7
	Mutagenesis	101	1	G → C in eid1-2; enhances sensitivity to red and far-red light. Ref.7
	Mutagenesis	197 – 198	2	RK → SE: Cytoplasmic localization instead of nuclear.
	Sequence conflict	16	1	N → K in AAM62754. Ref.5
	Sequence conflict	71	1	A → AA in AAM62754. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q8LEA8 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 0E4231CCF243487C	FASTA	336	37,398
	10 20 30 40 50 60 MAESVFSCIP EDVVFNIFFK LQDDPRNWAR LACVCTKFSS IVRNVCCKTQ CYSAIPTVIS 70 80 90 100 110 120 DLLPLPPSAA ASASSSTAAD SSLTPPGGWA SLYKLAVCCP GLFHAGILLE NSDFGLEREL 130 140 150 160 170 180 GPDQNLDPKP TTTDLALNDE EVSKPVGSGL ETTSFWSLYD DLYTDTIPAP PPEDSIDDQE 190 200 210 220 230 240 EEIETSEIRP GRDLPVRKRR KICRSLGSHL ASGGWNLSRE QGNKLLASRF RGDCLYICNW 250 260 270 280 290 300 PGCIHVEEKR NYMLFRGVFK DFKRSRVWRT INDGNRSKTS GLKCAFCLCD ETWDLHSSFC 310 320 330 LRRVFGFHDD GEPVVRAYVC ENGHVSGAWT ALPLYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"eid1: a new Arabidopsis mutant hypersensitive in phytochrome A-dependent high-irradiance responses." Bueche C., Poppe C., Schaefer E., Kretsch T. Plant Cell 12:547-558(2000) [PubMed: 10760243] [Abstract] Cited for: FUNCTION.
[7]	"EID1, an F-box protein involved in phytochrome A-specific light signaling." Dieterle M., Zhou Y.-C., Schaefer E., Funk M., Kretsch T. Genes Dev. 15:939-944(2001) [PubMed: 11316788] [Abstract] Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, INTERACTION WITH SKP1A AND SKP1B, DOMAIN, MUTAGENESIS OF PRO-10; GLY-101 AND 197-ARG-LYS-198.
[8]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069298 Genomic DNA. Translation: AAC19270.1. AL161494 Genomic DNA. Translation: CAB80737.1. CP002687 Genomic DNA. Translation: AEE82172.1. BT002038 mRNA. Translation: AAN72049.1. BT008731 mRNA. Translation: AAP42744.1. AK229833 mRNA. Translation: BAF01662.1. AY085530 mRNA. Translation: AAM62754.1.
IPI	IPI00516571.
PIR	T01306.
RefSeq	NP_192153.1. NM_116477.1.
UniGene	At.3886. At.43977.
3D structure databases
ProteinModelPortal	Q8LEA8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8LEA8. 9 interactions.
STRING	Q8LEA8.
Proteomic databases
PRIDE	Q8LEA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G02440.1; AT4G02440.1; AT4G02440.
GeneID	828008.
GenomeReviews	Gene locus AT4G02440 in contig CT486007_GR.
KEGG	ath:AT4G02440.
NMPDR	fig|3702.1.peg.18057.
Organism-specific databases
TAIR	At4g02440.
Phylogenomic databases
GeneTree	EPGT00070000030765.
HOGENOM	HBG605655.
InParanoid	Q8LEA8.
OMA	SREQGNK.
PhylomeDB	Q8LEA8.
ProtClustDB	CLSN2685743.
Gene expression databases
Genevestigator	Q8LEA8.
GermOnline	AT4G02440. Arabidopsis thaliana.
Family and domain databases
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EID1_ARATH

Accession

Primary (citable) accession number: Q8LEA8
Secondary accession number(s): O81289, Q0WMI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	December 14, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program