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Protein

Glutathione S-transferase DHAR3, chloroplastic

Gene

DHAR3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities (PubMed:12077129). Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses (By similarity).By similarity1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication

Kineticsi

  1. KM=0.50 mM for DHA (at pH 6.5 and 30 degrees Celsius)1 Publication
  2. KM=10 mM for GSH (at pH 6.5 and 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei54AscorbateBy similarity1
    Binding sitei54GlutathioneBy similarity1
    Binding sitei65AscorbateBy similarity1
    Binding sitei65GlutathioneBy similarity1
    Active sitei66NucleophileBy similarity1
    Binding sitei93GlutathioneBy similarity1
    Binding sitei106Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei119GlutathioneBy similarity1
    Binding sitei205GlutathioneBy similarity1
    Binding sitei252GlutathioneBy similarity1
    Binding sitei255AscorbateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • glutathione metabolic process Source: GO_Central
    • protein glutathionylation Source: TAIR
    • response to cytokinin Source: TAIR

    Keywordsi

    Molecular functionOxidoreductase, Transferase
    Biological processDetoxification

    Enzyme and pathway databases

    BioCyciARA:AT5G16710-MONOMER
    SABIO-RKiQ8LE52

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase DHAR3, chloroplastic (EC:2.5.1.181 Publication)
    Alternative name(s):
    Chloride intracellular channel homolog 3
    Short name:
    CLIC homolog 3
    Glutathione-dependent dehydroascorbate reductase 31 Publication (EC:1.8.5.11 Publication)
    Short name:
    AtDHAR31 Publication
    Short name:
    ChlDHAR
    Short name:
    GSH-dependent dehydroascorbate reductase 3
    Gene namesi
    Name:DHAR3
    Ordered Locus Names:At5g16710
    ORF Names:F5E19.50
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G16710
    TAIRilocus:2149015 AT5G16710

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 42ChloroplastSequence analysisAdd BLAST42
    ChainiPRO_000039548343 – 258Glutathione S-transferase DHAR3, chloroplasticAdd BLAST216

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei52S-glutathionyl cysteine1 Publication1
    Disulfide bondi66 ↔ 69In soluble form

    Post-translational modificationi

    Partial S-glutathionylation and intramolecular disulfide bond formation between Cys-66 and Cys-69 in the presence of oxidized glutathione (GSSG). Could be reduced by TRX-dependent process.1 Publication

    Keywords - PTMi

    Disulfide bond, Glutathionylation

    Proteomic databases

    PaxDbiQ8LE52
    PRIDEiQ8LE52

    PTM databases

    iPTMnetiQ8LE52

    Expressioni

    Gene expression databases

    ExpressionAtlasiQ8LE52 baseline and differential
    GenevisibleiQ8LE52 AT

    Interactioni

    Subunit structurei

    Monomer (By similarity). Interacts with TRX3.By similarity1 Publication

    Protein-protein interaction databases

    IntActiQ8LE52, 1 interactor
    STRINGi3702.AT5G16710.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LE52
    SMRiQ8LE52
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini56 – 129GST N-terminalAdd BLAST74
    Domaini130 – 258GST C-terminalAdd BLAST129

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi66 – 71Glutathione-bindingSequence analysis6

    Sequence similaritiesi

    Belongs to the GST superfamily. DHAR family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiENOG410INGY Eukaryota
    ENOG4110FKA LUCA
    HOGENOMiHOG000272670
    InParanoidiQ8LE52
    KOiK21888
    OMAiANGDDRD
    OrthoDBiEOG09360IQR
    PhylomeDBiQ8LE52

    Family and domain databases

    InterProiView protein in InterPro
    IPR010987 Glutathione-S-Trfase_C-like
    IPR036282 Glutathione-S-Trfase_C_sf
    IPR004045 Glutathione_S-Trfase_N
    IPR036249 Thioredoxin-like_sf
    PfamiView protein in Pfam
    PF13417 GST_N_3, 1 hit
    SUPFAMiSSF47616 SSF47616, 1 hit
    SSF52833 SSF52833, 1 hit
    PROSITEiView protein in PROSITE
    PS50405 GST_CTER, 1 hit
    PS50404 GST_NTER, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LE52-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MISLRFQPST TAGVLSASVS RAGFIKRCGS TKPGRVGRFV TMATAASPLE
    60 70 80 90 100
    ICVKASITTP NKLGDCPFCQ KVLLTMEEKN VPYDMKMVDL SNKPEWFLKI
    110 120 130 140 150
    SPEGKVPVVK FDEKWVPDSD VITQALEEKY PEPPLATPPE KASVGSKIFS
    160 170 180 190 200
    TFVGFLKSKD SGDGTEQVLL DELTTFNDYI KDNGPFINGE KISAADLSLA
    210 220 230 240 250
    PKLYHMKIAL GHYKNWSVPD SLPFVKSYME NVFSRESFTN TRAETEDVIA

    GWRPKVMG
    Length:258
    Mass (Da):28,514
    Last modified:October 1, 2002 - v1
    Checksum:i35AC1A8EFDD3E77C
    GO

    Sequence cautioni

    The sequence CAC01835 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At5g16710 and At5g16715.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti65D → Y in AAL38300 (PubMed:14593172).Curated1
    Sequence conflicti65D → Y in AAN65072 (PubMed:14593172).Curated1
    Sequence conflicti231N → D in BAD43518 (Ref. 5) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL391147 Genomic DNA Translation: CAC01835.1 Sequence problems.
    CP002688 Genomic DNA Translation: AED92328.1
    AK118603 mRNA Translation: BAC43202.1
    AY065124 mRNA Translation: AAL38300.1
    BT001185 mRNA Translation: AAN65072.1
    AK175537 mRNA Translation: BAD43300.1
    AK175755 mRNA Translation: BAD43518.1
    AK175798 mRNA Translation: BAD43561.1
    AK176039 mRNA Translation: BAD43802.1
    AK176071 mRNA Translation: BAD43834.1
    AK176708 mRNA Translation: BAD44471.1
    AK176820 mRNA Translation: BAD44583.1
    AK176870 mRNA Translation: BAD44633.1
    AY085616 mRNA Translation: AAM62837.1
    AF195887 mRNA Translation: AAG24946.1
    AF301597 mRNA Translation: AAG40196.1
    PIRiT51503
    RefSeqiNP_568336.1, NM_121676.4
    UniGeneiAt.16881

    Genome annotation databases

    EnsemblPlantsiAT5G16710.1; AT5G16710.1; AT5G16710
    GeneIDi831532
    GrameneiAT5G16710.1; AT5G16710.1; AT5G16710
    KEGGiath:AT5G16710

    Similar proteinsi

    Entry informationi

    Entry nameiDHAR3_ARATH
    AccessioniPrimary (citable) accession number: Q8LE52
    Secondary accession number(s): Q67XJ9
    , Q680W2, Q8VZA3, Q9FE30, Q9LFE6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: October 1, 2002
    Last modified: April 25, 2018
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families
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