Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8LDW9

- XTH9_ARATH

UniProt

Q8LDW9 - XTH9_ARATH

Protein

Xyloglucan endotransglucosylase/hydrolase protein 9

Gene

XTH9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (28 Nov 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues. Involved in internodal cell elongation.1 Publication

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei101 – 1011NucleophileBy similarity
    Active sitei105 – 1051Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT4G03210-MONOMER.
    ARA:GQT-624-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 9 (EC:2.4.1.207)
    Short name:
    At-XTH9
    Short name:
    XTH-9
    Gene namesi
    Name:XTH9
    Synonyms:EXGT-A6, XTR16
    Ordered Locus Names:At4g03210
    ORF Names:F4C21.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G03210.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 290264Xyloglucan endotransglucosylase/hydrolase protein 9PRO_0000011809Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi55 – 551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8LDW9.
    PRIDEiQ8LDW9.

    Expressioni

    Tissue specificityi

    Highly expressed in shoot apices. In the vegetative and reproductive phases, it accumulates in the shoot apex region, where cell division is most active. In the reproductive phase, it is also expressed in flower buds, flower stalks and internodes bearing flowers.2 Publications

    Gene expression databases

    ArrayExpressiQ8LDW9.
    GenevestigatoriQ8LDW9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LDW9.
    SMRiQ8LDW9. Positions 29-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ8LDW9.
    KOiK08235.
    OMAiANHMVYD.
    PhylomeDBiQ8LDW9.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8LDW9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVGMDLFKCV MMIMVLVVSC GEAVSGAKFD ELYRSSWAMD HCVNEGEVTK    50
    LKLDNYSGAG FESRSKYLFG KVSIQIKLVE GDSAGTVTAF YMSSDGPNHN 100
    EFDFEFLGNT TGEPYIVQTN IYVNGVGNRE QRLNLWFDPT TEFHTYSILW 150
    SKRSVVFMVD ETPIRVQKNL EEKGIPFAKD QAMGVYSSIW NADDWATQGG 200
    LVKTDWSHAP FVASYKEFQI DACEIPTTTD LSKCNGDQKF WWDEPTVSEL 250
    SLHQNHQLIW VRANHMIYDY CFDATRFPVT PLECQHHRHL 290
    Length:290
    Mass (Da):33,141
    Last modified:November 28, 2003 - v2
    Checksum:iD5A3DA08A97C0E69
    GO

    Sequence cautioni

    The sequence AAM62971.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005275 Genomic DNA. Translation: AAD14449.1.
    AL161496 Genomic DNA. Translation: CAB77806.1.
    CP002687 Genomic DNA. Translation: AEE82292.1.
    AY044333 mRNA. Translation: AAK73274.1.
    AY072353 mRNA. Translation: AAL62345.1.
    BT002199 mRNA. Translation: AAN72210.1.
    AY085753 mRNA. Translation: AAM62971.1. Different initiation.
    PIRiG85040.
    RefSeqiNP_192230.1. NM_116559.2. [Q8LDW9-1]
    UniGeneiAt.3932.
    At.5453.

    Genome annotation databases

    EnsemblPlantsiAT4G03210.1; AT4G03210.1; AT4G03210. [Q8LDW9-1]
    GeneIDi828024.
    KEGGiath:AT4G03210.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC005275 Genomic DNA. Translation: AAD14449.1 .
    AL161496 Genomic DNA. Translation: CAB77806.1 .
    CP002687 Genomic DNA. Translation: AEE82292.1 .
    AY044333 mRNA. Translation: AAK73274.1 .
    AY072353 mRNA. Translation: AAL62345.1 .
    BT002199 mRNA. Translation: AAN72210.1 .
    AY085753 mRNA. Translation: AAM62971.1 . Different initiation.
    PIRi G85040.
    RefSeqi NP_192230.1. NM_116559.2. [Q8LDW9-1 ]
    UniGenei At.3932.
    At.5453.

    3D structure databases

    ProteinModelPortali Q8LDW9.
    SMRi Q8LDW9. Positions 29-290.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi Q8LDW9.
    PRIDEi Q8LDW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G03210.1 ; AT4G03210.1 ; AT4G03210 . [Q8LDW9-1 ]
    GeneIDi 828024.
    KEGGi ath:AT4G03210.

    Organism-specific databases

    TAIRi AT4G03210.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q8LDW9.
    KOi K08235.
    OMAi ANHMVYD.
    PhylomeDBi Q8LDW9.

    Enzyme and pathway databases

    BioCyci ARA:AT4G03210-MONOMER.
    ARA:GQT-624-MONOMER.

    Gene expression databases

    ArrayExpressi Q8LDW9.
    Genevestigatori Q8LDW9.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Systematic isolation of highly transcribed genes in inflorescence apices in Arabidopsis thaliana from an equalized cDNA library."
      Hyodo H., Takemura M., Yokota A., Ohyama K., Kohchi T.
      Biosci. Biotechnol. Biochem. 64:1538-1541(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Flower.
    6. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.
    8. "Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana."
      Hyodo H., Yamakawa S., Takeda Y., Tsuduki M., Yokota A., Nishitani K., Kohchi T.
      Plant Mol. Biol. 52:473-482(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiXTH9_ARATH
    AccessioniPrimary (citable) accession number: Q8LDW9
    Secondary accession number(s): Q9ZR10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 28, 2003
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3