Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot Q8LDU4 (RCCR_ARATH)

Last modified June 16, 2009. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Red chlorophyll catabolite reductase, chloroplastic
      Short name=RCC reductase
      Short name=AtRCCR
    EC=1.3.1.80
Alternative name(s):
    Accelerated cell death protein 2
Gene names
Name: RCCR
Synonyms: ACD2
Ordered Locus Names: At4g37000
ORF Names: C7A10_360
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Catalyzes the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC). Works in a two-step reaction with pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of the intermediate, RCC. Ref.1

Catalytic activity

Primary fluorescent chlorophyll catabolite + NADP+ = red chlorophyll catabolite + NADPH.

Pathway

Porphyrin degradation; chlorophyll degradation.

Subcellular location

Plastid › chloroplast stroma. Note: And a low amount in mitochondria of 7-day-old seedlings. Ref.1 Ref.2

Tissue specificity

Expressed in all tissues tested, including roots.

Developmental stage

Present at all times of development. No change of levels during senescence or pathogen attack.

Miscellaneous

The absence of light completely suppresses cell death in acd2 mutants.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Chloroplast Potential
Chain40 – 319280Red chlorophyll catabolite reductase, chloroplastic
PRO_0000022201

Regions

Coiled coil255 – 28632 Potential

Experimental info

Mutagenesis1401G → V in acd2-12E13; spontaneous spreading cell death lesions. Ref.2
Mutagenesis181 – 19212Missing in acd2-7; spontaneous spreading cell death lesions. Ref.2
Mutagenesis2791R → K in acd2-6; spontaneous spreading cell death lesions. Ref.2
Sequence conflict2901D → E in AAM63013. Ref.5

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q8LDU4-1 [UniParc].

Last modified May 16, 2003. Version 2.
Checksum: A46DC65FB7452517

FASTA31936,449
        10         20         30         40         50         60 
MAMIFCNTLY SSSSPSYLSP LTSKPSRFSK NLRPRAQFQS MEDHDDHLRR KFMEFPYVSP 

        70         80         90        100        110        120 
TRKQLMVDLM STVENRLQSQ LLPCNLPPDV RNFNNPNGSA EASLHIRSGD KSSPIDFVIG 

       130        140        150        160        170        180 
SWIHCKIPTG VSLNITSISG FLNSSTKAPN FVVELIQSSS KSLVLILDLP HRKDLVLNPD 

       190        200        210        220        230        240 
YLKEYYQDTA LDSHRQSLLK LPEVNPYVSP SLFVRSAFSP TASMLKIDAE EEDKLEEILR 

       250        260        270        280        290        300 
DHVSPAAKEV LEVWLERCVK EEEEKIVVGE EERMELERRD KSFRRKSIED DLDLQFPRMF 

       310 
GEEVSSRVVH AIKEAFGVL

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Molecular cloning, functional expression and characterization of RCC reductase, involved in chlorophyll catabolism."
Wuethrich K.L., Bovet L., Hunziker P.E., Donnison I.S., Hoertensteiner S.
Plant J. 21:189-198(2000) [PubMed: 10743659] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[2]"The Arabidopsis accelerated cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms."
Mach J.M., Castillo A.R., Hoogstraten R., Greenberg J.T.
Proc. Natl. Acad. Sci. U.S.A. 98:771-776(2001) [PubMed: 11149948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-140; 181-TYR--ASP-192 AND ARG-279.
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Hide | Top

Cross-references

Sequence databases

AF326347 mRNA. Translation: AAG53980.1.
Z99707 Genomic DNA. Translation: CAB16763.1.
AL161590 Genomic DNA. Translation: CAB80366.1.
AY045578 mRNA. Translation: AAK73936.1.
AY093785 mRNA. Translation: AAM10401.1.
AY085797 mRNA. Translation: AAM63013.1.
IPIIPI00538166.
PIRA85437.
RefSeqNP_195417.1.
UniGeneAt.4644

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2ZXKX-ray2.50A/B40-319[»]
2ZXLX-ray2.40A/B40-319[»]
ModBaseSearch...

Proteomic databases

PRIDEQ8LDU4.

Genome annotation databases

GeneID829854.
GenomeReviewsGene locus AT4G37000 in contig CT486007_GR.
KEGGath:AT4G37000.
NMPDRfig|3702.1.peg.21804.

Organism-specific databases

TAIRAt4g37000.

Phylogenomic databases

OMAQ8LDU4. GWVDEAE.

Enzyme and pathway databases

BRENDA1.3.1.80. 302.

Gene expression databases

ArrayExpressQ8LDU4.
GermOnlineAT4G37000. Arabidopsis thaliana.

Family and domain databases

InterProIPR009439. RCC_reductase.
[Graphical view]
PfamPF06405. RCC_reductase. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameRCCR_ARATH
AccessionPrimary (citable) accession number: Q8LDU4
Secondary accession number(s): O23185
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 16, 2003
Last modified: June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents