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Q8LDS2

- XTH27_ARATH

UniProt

Q8LDS2 - XTH27_ARATH

Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 27

Gene

XTH27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (28 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity. Required for cell wall modification during the development of tracheary elements.By similarity1 Publication

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081NucleophileBy similarity
    Active sitei112 – 1121Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. phloem or xylem histogenesis Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT2G01850-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase protein 27 (EC:2.4.1.207)
    Short name:
    At-XTH27
    Short name:
    XTH-27
    Gene namesi
    Name:XTH27
    Synonyms:EXGT-A3
    Ordered Locus Names:At2g01850
    ORF Names:T23K3.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G01850.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    In xth27-1 and xth27-2, short-shaped tracheary elements in tertiary veins, reduced number of tertiary veins in the first leaf, and yellow lesion-mimic spots in mature rosette leaves.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 333313Probable xyloglucan endotransglucosylase/hydrolase protein 27PRO_0000011827Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in 7 day old seedlings, roots, hypocotyls, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds, anthers and siliques.2 Publications

    Developmental stagei

    Accumulates in the immature tracheary elements of rosette leaves. Expressed in differentiating vasculature of the root, the hypocotyls, and the flower filaments, as well as in the anthers and the inner subepidermal layer of mature siliques.1 Publication

    Gene expression databases

    ArrayExpressiQ8LDS2.
    GenevestigatoriQ8LDS2.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G01850.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LDS2.
    SMRiQ8LDS2. Positions 31-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    InParanoidiQ8LDS2.
    KOiK08235.
    OMAiNGDMYEK.
    PhylomeDBiQ8LDS2.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8LDS2-1 [UniParc]FASTAAdd to Basket

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    METLSRLLVF MSLFSGLVSG FALQNLPITS FEESYTQLFG DKNLFVHQDG    50
    KSVRLTLDER TGSGFVSNDY YLHGFFSASI KLPSDYTAGV VVAFYMSNGD 100
    MYEKNHDEID FEFLGNIREK EWRVQTNIYG NGSTHSGREE RYNLWFDPTE 150
    DFHQYSILWS DSHIIFFVDN VPIREVKRTA EMGGHFPSKP MSLYTTIWDG 200
    SKWATNGGKY GVNYKYAPYI ARFSDLVLHG CPVDPIEQFP RCDEGAAEDM 250
    RAAQEITPSQ RSKMDVFRRR LMTYSYCYDR ARYNVALSEC VVNPAEAQRL 300
    RVYDPVRFGG IPRRHRNGKH RSKRSRVDGT ESI 333
    Length:333
    Mass (Da):38,415
    Last modified:November 28, 2003 - v2
    Checksum:iE5B9861A9E5989D9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti181 – 1811E → Q in AAD45125. (PubMed:10557219)Curated
    Sequence conflicti250 – 2501M → I in AAM63050. 1 PublicationCurated
    Sequence conflicti331 – 3311E → K in AAM63050. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF163821 Genomic DNA. Translation: AAD45125.1.
    D63509 mRNA. Translation: BAA20289.1.
    AC007069 Genomic DNA. Translation: AAD21783.1.
    CP002685 Genomic DNA. Translation: AEC05508.1.
    AY059910 mRNA. Translation: AAL24392.1.
    BT008820 mRNA. Translation: AAP68259.1.
    AY085835 mRNA. Translation: AAM63050.1.
    AK317757 mRNA. Translation: BAH20413.1.
    PIRiH84429.
    RefSeqiNP_178294.1. NM_126246.3.
    UniGeneiAt.21536.

    Genome annotation databases

    EnsemblPlantsiAT2G01850.1; AT2G01850.1; AT2G01850.
    GeneIDi814716.
    KEGGiath:AT2G01850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF163821 Genomic DNA. Translation: AAD45125.1 .
    D63509 mRNA. Translation: BAA20289.1 .
    AC007069 Genomic DNA. Translation: AAD21783.1 .
    CP002685 Genomic DNA. Translation: AEC05508.1 .
    AY059910 mRNA. Translation: AAL24392.1 .
    BT008820 mRNA. Translation: AAP68259.1 .
    AY085835 mRNA. Translation: AAM63050.1 .
    AK317757 mRNA. Translation: BAH20413.1 .
    PIRi H84429.
    RefSeqi NP_178294.1. NM_126246.3.
    UniGenei At.21536.

    3D structure databases

    ProteinModelPortali Q8LDS2.
    SMRi Q8LDS2. Positions 31-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G01850.1-P.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G01850.1 ; AT2G01850.1 ; AT2G01850 .
    GeneIDi 814716.
    KEGGi ath:AT2G01850.

    Organism-specific databases

    GeneFarmi 2643. 265.
    TAIRi AT2G01850.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    InParanoidi Q8LDS2.
    KOi K08235.
    OMAi NGDMYEK.
    PhylomeDBi Q8LDS2.

    Enzyme and pathway databases

    BioCyci ARA:AT2G01850-MONOMER.

    Gene expression databases

    ArrayExpressi Q8LDS2.
    Genevestigatori Q8LDS2.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
      Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
      Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. Okamoto S., Kamimai T., Nishitani K.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-333.
      Strain: cv. Columbia.
      Tissue: Rosette leaf.
    8. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.
    9. "AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements."
      Matsui A., Yokoyama R., Seki M., Ito T., Shinozaki K., Takahashi T., Komeda Y., Nishitani K.
      Plant J. 42:525-534(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
      Strain: cv. Columbia and cv. No-0.

    Entry informationi

    Entry nameiXTH27_ARATH
    AccessioniPrimary (citable) accession number: Q8LDS2
    Secondary accession number(s): B9DI46, O04906, Q9SEB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 28, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3