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Q8LDS2 (XTH27_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 27

Short name=At-XTH27
Short name=XTH-27
EC=2.4.1.207
Gene names
Name:XTH27
Synonyms:EXGT-A3
Ordered Locus Names:At2g01850
ORF Names:T23K3.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Expressed in 7 day old seedlings, roots, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds and siliques. Ref.1

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 3 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 333313Probable xyloglucan endotransglucosylase/hydrolase protein 27
PRO_0000011827

Sites

Active site1081Nucleophile By similarity
Active site1121Proton donor By similarity

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1811E → Q in AAD45125. Ref.1
Sequence conflict2501M → I in AAM63050. Ref.6
Sequence conflict3311E → K in AAM63050. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q8LDS2 [UniParc].

Last modified November 28, 2003. Version 2.
Checksum: E5B9861A9E5989D9

FASTA33338,415
        10         20         30         40         50         60 
METLSRLLVF MSLFSGLVSG FALQNLPITS FEESYTQLFG DKNLFVHQDG KSVRLTLDER 

        70         80         90        100        110        120 
TGSGFVSNDY YLHGFFSASI KLPSDYTAGV VVAFYMSNGD MYEKNHDEID FEFLGNIREK 

       130        140        150        160        170        180 
EWRVQTNIYG NGSTHSGREE RYNLWFDPTE DFHQYSILWS DSHIIFFVDN VPIREVKRTA 

       190        200        210        220        230        240 
EMGGHFPSKP MSLYTTIWDG SKWATNGGKY GVNYKYAPYI ARFSDLVLHG CPVDPIEQFP 

       250        260        270        280        290        300 
RCDEGAAEDM RAAQEITPSQ RSKMDVFRRR LMTYSYCYDR ARYNVALSEC VVNPAEAQRL 

       310        320        330 
RVYDPVRFGG IPRRHRNGKH RSKRSRVDGT ESI 

« Hide

References

« Hide 'large scale' references
[1]"Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]Okamoto S., Kamimai T., Nishitani K.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF163821 Genomic DNA. Translation: AAD45125.1.
D63509 mRNA. Translation: BAA20289.1.
AC007069 Genomic DNA. Translation: AAD21783.1.
CP002685 Genomic DNA. Translation: AEC05508.1.
AY059910 mRNA. Translation: AAL24392.1.
BT008820 mRNA. Translation: AAP68259.1.
AY085835 mRNA. Translation: AAM63050.1.
PIRH84429.
RefSeqNP_178294.1. NM_126246.3.
UniGeneAt.21536.

3D structure databases

ProteinModelPortalQ8LDS2.
SMRQ8LDS2. Positions 31-290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G01850.1-P.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G01850.1; AT2G01850.1; AT2G01850.
GeneID814716.
KEGGath:AT2G01850.

Organism-specific databases

GeneFarm2643. 265.
TAIRAT2G01850.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
InParanoidQ8LDS2.
KOK08235.
OMAKRVHISL.
PhylomeDBQ8LDS2.
ProtClustDBCLSN2682977.

Enzyme and pathway databases

BioCycARA:AT2G01850-MONOMER.

Gene expression databases

ArrayExpressQ8LDS2.
GenevestigatorQ8LDS2.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameXTH27_ARATH
AccessionPrimary (citable) accession number: Q8LDS2
Secondary accession number(s): O04906, Q9SEB1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: February 19, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names