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Q8LDS2

- XTH27_ARATH

UniProt

Q8LDS2 - XTH27_ARATH

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Protein
Probable xyloglucan endotransglucosylase/hydrolase protein 27
Gene
XTH27, EXGT-A3, At2g01850, T23K3.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081Nucleophile By similarity
Active sitei112 – 1121Proton donor By similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. phloem or xylem histogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G01850-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 27 (EC:2.4.1.207)
Short name:
At-XTH27
Short name:
XTH-27
Gene namesi
Name:XTH27
Synonyms:EXGT-A3
Ordered Locus Names:At2g01850
ORF Names:T23K3.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G01850.

Subcellular locationi

Secretedcell wall Inferred. Secretedextracellular spaceapoplast Inferred

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 333313Probable xyloglucan endotransglucosylase/hydrolase protein 27
PRO_0000011827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in 7 day old seedlings, roots, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds and siliques.1 Publication

Gene expression databases

ArrayExpressiQ8LDS2.
GenevestigatoriQ8LDS2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G01850.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LDS2.
SMRiQ8LDS2. Positions 31-290.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ8LDS2.
KOiK08235.
OMAiNGDMYEK.
PhylomeDBiQ8LDS2.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LDS2-1 [UniParc]FASTAAdd to Basket

« Hide

METLSRLLVF MSLFSGLVSG FALQNLPITS FEESYTQLFG DKNLFVHQDG    50
KSVRLTLDER TGSGFVSNDY YLHGFFSASI KLPSDYTAGV VVAFYMSNGD 100
MYEKNHDEID FEFLGNIREK EWRVQTNIYG NGSTHSGREE RYNLWFDPTE 150
DFHQYSILWS DSHIIFFVDN VPIREVKRTA EMGGHFPSKP MSLYTTIWDG 200
SKWATNGGKY GVNYKYAPYI ARFSDLVLHG CPVDPIEQFP RCDEGAAEDM 250
RAAQEITPSQ RSKMDVFRRR LMTYSYCYDR ARYNVALSEC VVNPAEAQRL 300
RVYDPVRFGG IPRRHRNGKH RSKRSRVDGT ESI 333
Length:333
Mass (Da):38,415
Last modified:November 28, 2003 - v2
Checksum:iE5B9861A9E5989D9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811E → Q in AAD45125. 1 Publication
Sequence conflicti250 – 2501M → I in AAM63050. 1 Publication
Sequence conflicti331 – 3311E → K in AAM63050. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163821 Genomic DNA. Translation: AAD45125.1.
D63509 mRNA. Translation: BAA20289.1.
AC007069 Genomic DNA. Translation: AAD21783.1.
CP002685 Genomic DNA. Translation: AEC05508.1.
AY059910 mRNA. Translation: AAL24392.1.
BT008820 mRNA. Translation: AAP68259.1.
AY085835 mRNA. Translation: AAM63050.1.
PIRiH84429.
RefSeqiNP_178294.1. NM_126246.3.
UniGeneiAt.21536.

Genome annotation databases

EnsemblPlantsiAT2G01850.1; AT2G01850.1; AT2G01850.
GeneIDi814716.
KEGGiath:AT2G01850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF163821 Genomic DNA. Translation: AAD45125.1 .
D63509 mRNA. Translation: BAA20289.1 .
AC007069 Genomic DNA. Translation: AAD21783.1 .
CP002685 Genomic DNA. Translation: AEC05508.1 .
AY059910 mRNA. Translation: AAL24392.1 .
BT008820 mRNA. Translation: AAP68259.1 .
AY085835 mRNA. Translation: AAM63050.1 .
PIRi H84429.
RefSeqi NP_178294.1. NM_126246.3.
UniGenei At.21536.

3D structure databases

ProteinModelPortali Q8LDS2.
SMRi Q8LDS2. Positions 31-290.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G01850.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G01850.1 ; AT2G01850.1 ; AT2G01850 .
GeneIDi 814716.
KEGGi ath:AT2G01850.

Organism-specific databases

GeneFarmi 2643. 265.
TAIRi AT2G01850.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000236368.
InParanoidi Q8LDS2.
KOi K08235.
OMAi NGDMYEK.
PhylomeDBi Q8LDS2.

Enzyme and pathway databases

BioCyci ARA:AT2G01850-MONOMER.

Gene expression databases

ArrayExpressi Q8LDS2.
Genevestigatori Q8LDS2.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
    Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
    Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Okamoto S., Kamimai T., Nishitani K.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH27_ARATH
AccessioniPrimary (citable) accession number: Q8LDS2
Secondary accession number(s): O04906, Q9SEB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 14, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi