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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 27

Gene

XTH27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity). Required for cell wall modification during the development of tracheary elements.By similarity1 Publication

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081NucleophileBy similarity
Active sitei112 – 1121Proton donorBy similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. phloem or xylem histogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G01850-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 27 (EC:2.4.1.207)
Short name:
At-XTH27
Short name:
XTH-27
Gene namesi
Name:XTH27
Synonyms:EXGT-A3
Ordered Locus Names:At2g01850
ORF Names:T23K3.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G01850.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
  2. cell wall Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

In xth27-1 and xth27-2, short-shaped tracheary elements in tertiary veins, reduced number of tertiary veins in the first leaf, and yellow lesion-mimic spots in mature rosette leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 333313Probable xyloglucan endotransglucosylase/hydrolase protein 27PRO_0000011827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in 7 day old seedlings, roots, hypocotyls, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds, anthers and siliques.2 Publications

Developmental stagei

Accumulates in the immature tracheary elements of rosette leaves. Expressed in differentiating vasculature of the root, the hypocotyls, and the flower filaments, as well as in the anthers and the inner subepidermal layer of mature siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8LDS2. baseline and differential.
GenevestigatoriQ8LDS2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G01850.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8LDS2.
SMRiQ8LDS2. Positions 31-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ8LDS2.
KOiK08235.
OMAiNIREKEW.
PhylomeDBiQ8LDS2.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LDS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLSRLLVF MSLFSGLVSG FALQNLPITS FEESYTQLFG DKNLFVHQDG
60 70 80 90 100
KSVRLTLDER TGSGFVSNDY YLHGFFSASI KLPSDYTAGV VVAFYMSNGD
110 120 130 140 150
MYEKNHDEID FEFLGNIREK EWRVQTNIYG NGSTHSGREE RYNLWFDPTE
160 170 180 190 200
DFHQYSILWS DSHIIFFVDN VPIREVKRTA EMGGHFPSKP MSLYTTIWDG
210 220 230 240 250
SKWATNGGKY GVNYKYAPYI ARFSDLVLHG CPVDPIEQFP RCDEGAAEDM
260 270 280 290 300
RAAQEITPSQ RSKMDVFRRR LMTYSYCYDR ARYNVALSEC VVNPAEAQRL
310 320 330
RVYDPVRFGG IPRRHRNGKH RSKRSRVDGT ESI
Length:333
Mass (Da):38,415
Last modified:November 28, 2003 - v2
Checksum:iE5B9861A9E5989D9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811E → Q in AAD45125 (PubMed:10557219).Curated
Sequence conflicti250 – 2501M → I in AAM63050 (Ref. 6) Curated
Sequence conflicti331 – 3311E → K in AAM63050 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163821 Genomic DNA. Translation: AAD45125.1.
D63509 mRNA. Translation: BAA20289.1.
AC007069 Genomic DNA. Translation: AAD21783.1.
CP002685 Genomic DNA. Translation: AEC05508.1.
AY059910 mRNA. Translation: AAL24392.1.
BT008820 mRNA. Translation: AAP68259.1.
AY085835 mRNA. Translation: AAM63050.1.
AK317757 mRNA. Translation: BAH20413.1.
PIRiH84429.
RefSeqiNP_178294.1. NM_126246.3.
UniGeneiAt.21536.

Genome annotation databases

EnsemblPlantsiAT2G01850.1; AT2G01850.1; AT2G01850.
GeneIDi814716.
KEGGiath:AT2G01850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163821 Genomic DNA. Translation: AAD45125.1.
D63509 mRNA. Translation: BAA20289.1.
AC007069 Genomic DNA. Translation: AAD21783.1.
CP002685 Genomic DNA. Translation: AEC05508.1.
AY059910 mRNA. Translation: AAL24392.1.
BT008820 mRNA. Translation: AAP68259.1.
AY085835 mRNA. Translation: AAM63050.1.
AK317757 mRNA. Translation: BAH20413.1.
PIRiH84429.
RefSeqiNP_178294.1. NM_126246.3.
UniGeneiAt.21536.

3D structure databases

ProteinModelPortaliQ8LDS2.
SMRiQ8LDS2. Positions 31-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G01850.1-P.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G01850.1; AT2G01850.1; AT2G01850.
GeneIDi814716.
KEGGiath:AT2G01850.

Organism-specific databases

GeneFarmi2643. 265.
TAIRiAT2G01850.

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ8LDS2.
KOiK08235.
OMAiNIREKEW.
PhylomeDBiQ8LDS2.

Enzyme and pathway databases

BioCyciARA:AT2G01850-MONOMER.

Miscellaneous databases

PROiQ8LDS2.

Gene expression databases

ExpressionAtlasiQ8LDS2. baseline and differential.
GenevestigatoriQ8LDS2.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis."
    Akamatsu T., Hanzawa Y., Ohtake Y., Takahashi T., Nishitani K., Komeda Y.
    Plant Physiol. 121:715-721(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Okamoto S., Kamimai T., Nishitani K.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-333.
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  8. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  9. "AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements."
    Matsui A., Yokoyama R., Seki M., Ito T., Shinozaki K., Takahashi T., Komeda Y., Nishitani K.
    Plant J. 42:525-534(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: cv. Columbia and cv. No-0.

Entry informationi

Entry nameiXTH27_ARATH
AccessioniPrimary (citable) accession number: Q8LDS2
Secondary accession number(s): B9DI46, O04906, Q9SEB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: April 29, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.