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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 27

Gene

XTH27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity). Required for cell wall modification during the development of tracheary elements.By similarity1 Publication

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108NucleophileBy similarity1
Active sitei112Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall biogenesis Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • phloem or xylem histogenesis Source: TAIR
  • xyloglucan metabolic process Source: InterPro

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G01850-MONOMER

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 27 (EC:2.4.1.207)
Short name:
At-XTH27
Short name:
XTH-27
Gene namesi
Name:XTH27
Synonyms:EXGT-A3
Ordered Locus Names:At2g01850
ORF Names:T23K3.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G01850
TAIRilocus:2059728 AT2G01850

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

In xth27-1 and xth27-2, short-shaped tracheary elements in tertiary veins, reduced number of tertiary veins in the first leaf, and yellow lesion-mimic spots in mature rosette leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001182721 – 333Probable xyloglucan endotransglucosylase/hydrolase protein 27Add BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8LDS2

Expressioni

Tissue specificityi

Expressed in 7 day old seedlings, roots, hypocotyls, rosette leaves, internodes between nodes bearing axillary shoots, nodes bearing flowers, flower buds, anthers and siliques.2 Publications

Developmental stagei

Accumulates in the immature tracheary elements of rosette leaves. Expressed in differentiating vasculature of the root, the hypocotyls, and the flower filaments, as well as in the anthers and the inner subepidermal layer of mature siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ8LDS2 baseline and differential
GenevisibleiQ8LDS2 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G01850.1

Structurei

3D structure databases

ProteinModelPortaliQ8LDS2
SMRiQ8LDS2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 223GH16PROSITE-ProRule annotationAdd BLAST203

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGGJ Eukaryota
COG2273 LUCA
HOGENOMiHOG000236368
InParanoidiQ8LDS2
KOiK08235
OMAiNIREKEW
OrthoDBiEOG09360GX8
PhylomeDBiQ8LDS2

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR010713 XET_C
IPR016455 XTH
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PF06955 XET_C, 1 hit
PIRSFiPIRSF005604 XET, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8LDS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLSRLLVF MSLFSGLVSG FALQNLPITS FEESYTQLFG DKNLFVHQDG
60 70 80 90 100
KSVRLTLDER TGSGFVSNDY YLHGFFSASI KLPSDYTAGV VVAFYMSNGD
110 120 130 140 150
MYEKNHDEID FEFLGNIREK EWRVQTNIYG NGSTHSGREE RYNLWFDPTE
160 170 180 190 200
DFHQYSILWS DSHIIFFVDN VPIREVKRTA EMGGHFPSKP MSLYTTIWDG
210 220 230 240 250
SKWATNGGKY GVNYKYAPYI ARFSDLVLHG CPVDPIEQFP RCDEGAAEDM
260 270 280 290 300
RAAQEITPSQ RSKMDVFRRR LMTYSYCYDR ARYNVALSEC VVNPAEAQRL
310 320 330
RVYDPVRFGG IPRRHRNGKH RSKRSRVDGT ESI
Length:333
Mass (Da):38,415
Last modified:November 28, 2003 - v2
Checksum:iE5B9861A9E5989D9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti181E → Q in AAD45125 (PubMed:10557219).Curated1
Sequence conflicti250M → I in AAM63050 (Ref. 6) Curated1
Sequence conflicti331E → K in AAM63050 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163821 Genomic DNA Translation: AAD45125.1
D63509 mRNA Translation: BAA20289.1
AC007069 Genomic DNA Translation: AAD21783.1
CP002685 Genomic DNA Translation: AEC05508.1
AY059910 mRNA Translation: AAL24392.1
BT008820 mRNA Translation: AAP68259.1
AY085835 mRNA Translation: AAM63050.1
AK317757 mRNA Translation: BAH20413.1
PIRiH84429
RefSeqiNP_178294.1, NM_126246.4
UniGeneiAt.21536

Genome annotation databases

EnsemblPlantsiAT2G01850.1; AT2G01850.1; AT2G01850
GeneIDi814716
GrameneiAT2G01850.1; AT2G01850.1; AT2G01850
KEGGiath:AT2G01850

Similar proteinsi

Entry informationi

Entry nameiXTH27_ARATH
AccessioniPrimary (citable) accession number: Q8LDS2
Secondary accession number(s): B9DI46, O04906, Q9SEB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: April 25, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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