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Protein

Proteasome subunit beta type-6

Gene

PBA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-382556 ABC-family proteins mediated transport
R-ATH-4641258 Degradation of DVL
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5668541 TNFR2 non-canonical NF-kB pathway
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.010

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-6 (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit A-1
Proteasome component D
Proteasome subunit beta type-1
Gene namesi
Name:PBA1
Synonyms:PRCD
Ordered Locus Names:At4g31300
ORF Names:F8F16.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G31300

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000428281 – 12Removed in mature formAdd BLAST12
ChainiPRO_000004282913 – 233Proteasome subunit beta type-6Add BLAST221

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ8LD27
PRIDEiQ8LD27

PTM databases

iPTMnetiQ8LD27

Expressioni

Gene expression databases

ExpressionAtlasiQ8LD27 baseline and differential
GenevisibleiQ8LD27 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi145433 interactors.
IntActiQ8LD27 2 interactors.
STRINGi3702.AT4G31300.2

Structurei

3D structure databases

ProteinModelPortaliQ8LD27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0174 Eukaryota
ENOG410XS23 LUCA
HOGENOMiHOG000091079
InParanoidiQ8LD27
KOiK02738
PhylomeDBiQ8LD27

Family and domain databases

Gene3Di3.60.20.101 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8LD27-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLNLDAPHS MGTTIIGVTY NGGVVLGADS RTSTGMYVAN RASDKITQLT
60 70 80 90 100
DNVYVCRSGS AADSQVVSDY VRYFLHQHTI QHGQPATVKV SANLIRMLAY
110 120 130 140 150
NNKNMLQTGL IVGGWDKYEG GKIYGIPLGG TVVEQPFAIG GSGSSYLYGF
160 170 180 190 200
FDQAWKDNMT KEEAEQLVVK AVSLAIARDG ASGGVVRTVI INSEGVTRNF
210 220 230
YPGDKLQLWH EELEPQNSLL DILNAAGPEP MAM
Length:233
Mass (Da):25,151
Last modified:November 22, 2005 - v2
Checksum:iD9DBB0CBD42623AB
GO

Sequence cautioni

The sequence AAM64316 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73Y → H in AAM64316 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13694 mRNA Translation: CAA74028.1
AF043529 mRNA Translation: AAC32065.1
AL021633 Genomic DNA Translation: CAA16533.1
AL161578 Genomic DNA Translation: CAB79848.1
CP002687 Genomic DNA Translation: AEE85887.1
CP002687 Genomic DNA Translation: AEE85889.1
AY052354 mRNA Translation: AAK96545.1
AY058240 mRNA Translation: AAL15414.1
AY086241 mRNA Translation: AAM64316.1 Different initiation.
PIRiT04497
RefSeqiNP_001190879.1, NM_001203950.1 [Q8LD27-1]
NP_194858.1, NM_119279.5 [Q8LD27-1]
UniGeneiAt.24496

Genome annotation databases

EnsemblPlantsiAT4G31300.1; AT4G31300.1; AT4G31300 [Q8LD27-1]
AT4G31300.3; AT4G31300.3; AT4G31300 [Q8LD27-1]
GeneIDi829257
GrameneiAT4G31300.1; AT4G31300.1; AT4G31300 [Q8LD27-1]
AT4G31300.3; AT4G31300.3; AT4G31300 [Q8LD27-1]
KEGGiath:AT4G31300

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB6_ARATH
AccessioniPrimary (citable) accession number: Q8LD27
Secondary accession number(s): O23716
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome