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Q8LCU7

- MECR_ARATH

UniProt

Q8LCU7 - MECR_ARATH

Protein

Probable trans-2-enoyl-CoA reductase, mitochondrial

Gene

At3g45770

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis By similarity.By similarity

    Catalytic activityi

    Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei169 – 1691NADPBy similarity
    Binding sitei370 – 3701NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi195 – 1984NADPBy similarity
    Nucleotide bindingi218 – 2203NADPBy similarity
    Nucleotide bindingi287 – 2904NADPBy similarity
    Nucleotide bindingi312 – 3143NADPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. copper ion binding Source: TAIR
    3. trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB-EC
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT3G45770-MONOMER.
    ARA:GQT-1388-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable trans-2-enoyl-CoA reductase, mitochondrial (EC:1.3.1.38)
    Gene namesi
    Ordered Locus Names:At3g45770
    ORF Names:T6D9.100
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G45770.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. mitochondrion Source: TAIR
    3. nucleus Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
    BLAST
    Chaini28 – 375348Probable trans-2-enoyl-CoA reductase, mitochondrialPRO_0000000895Add
    BLAST

    Proteomic databases

    PaxDbiQ8LCU7.
    PRIDEiQ8LCU7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8LCU7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi9040. 1 interaction.
    MINTiMINT-8069555.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LCU7.
    SMRiQ8LCU7. Positions 41-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0604.
    HOGENOMiHOG000294683.
    InParanoidiQ8LCU7.
    KOiK07512.
    OMAiCSTLWRV.
    PhylomeDBiQ8LCU7.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8LCU7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALMESVVG RALKFSSTAN FRSIRRGETP TLCIKSFSTI MSPPSKAIVY    50
    EEHGSPDSVT RLVNLPPVEV KENDVCVKMI AAPINPSDIN RIEGVYPVRP 100
    PVPAVGGYEG VGEVYAVGSN VNGFSPGDWV IPSPPSSGTW QTYVVKEESV 150
    WHKIDKECPM EYAATITVNP LTALRMLEDF VNLNSGDSVV QNGATSIVGQ 200
    CVIQLARLRG ISTINLIRDR AGSDEAREQL KALGADEVFS ESQLNVKNVK 250
    SLLGNLPEPA LGFNCVGGNA ASLVLKYLRE GGTMVTYGGM SKKPITVSTT 300
    SFIFKDLALR GFWLQSWLSM GKVKECREMI DYLLGLARDG KLKYETELVP 350
    FEEFPVALDK ALGKLGRQPK QVITF 375
    Length:375
    Mass (Da):40,823
    Last modified:October 1, 2002 - v1
    Checksum:i9047BCD122872F48
    GO

    Sequence cautioni

    The sequence CAB75790.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti293 – 2931K → E in AAL32632. (PubMed:14593172)Curated
    Sequence conflicti293 – 2931K → E in AAM47974. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL157735 Genomic DNA. Translation: CAB75790.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE78071.1.
    AY062554 mRNA. Translation: AAL32632.1.
    AY114655 mRNA. Translation: AAM47974.1.
    AY086398 mRNA. Translation: AAM64465.1.
    PIRiT47517.
    RefSeqiNP_566881.1. NM_114446.3. [Q8LCU7-1]
    UniGeneiAt.3719.

    Genome annotation databases

    EnsemblPlantsiAT3G45770.1; AT3G45770.1; AT3G45770. [Q8LCU7-1]
    GeneIDi823720.
    KEGGiath:AT3G45770.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL157735 Genomic DNA. Translation: CAB75790.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE78071.1 .
    AY062554 mRNA. Translation: AAL32632.1 .
    AY114655 mRNA. Translation: AAM47974.1 .
    AY086398 mRNA. Translation: AAM64465.1 .
    PIRi T47517.
    RefSeqi NP_566881.1. NM_114446.3. [Q8LCU7-1 ]
    UniGenei At.3719.

    3D structure databases

    ProteinModelPortali Q8LCU7.
    SMRi Q8LCU7. Positions 41-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 9040. 1 interaction.
    MINTi MINT-8069555.

    Proteomic databases

    PaxDbi Q8LCU7.
    PRIDEi Q8LCU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G45770.1 ; AT3G45770.1 ; AT3G45770 . [Q8LCU7-1 ]
    GeneIDi 823720.
    KEGGi ath:AT3G45770.

    Organism-specific databases

    GeneFarmi 2939.
    TAIRi AT3G45770.

    Phylogenomic databases

    eggNOGi COG0604.
    HOGENOMi HOG000294683.
    InParanoidi Q8LCU7.
    KOi K07512.
    OMAi CSTLWRV.
    PhylomeDBi Q8LCU7.

    Enzyme and pathway databases

    BioCyci ARA:AT3G45770-MONOMER.
    ARA:GQT-1388-MONOMER.

    Miscellaneous databases

    PROi Q8LCU7.

    Gene expression databases

    Genevestigatori Q8LCU7.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProi IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11695. PTHR11695. 1 hit.
    Pfami PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50129. SSF50129. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiMECR_ARATH
    AccessioniPrimary (citable) accession number: Q8LCU7
    Secondary accession number(s): Q8W4H6, Q9M166
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3