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Protein

Enoyl-[acyl-carrier-protein] reductase, mitochondrial

Gene

At3g45770

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro.By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Proton donorBy similarity1
Binding sitei169NADPBy similarity1
Binding sitei370NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi195 – 198NADPBy similarity4
Nucleotide bindingi218 – 220NADPBy similarity3
Nucleotide bindingi287 – 290NADPBy similarity4
Nucleotide bindingi312 – 314NADPBy similarity3

GO - Molecular functioni

  • ATP binding Source: TAIR
  • copper ion binding Source: TAIR
  • oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT3G45770-MONOMER.
ReactomeiR-ATH-77346. Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104)
Alternative name(s):
2-enoyl thioester reductase
Gene namesi
Ordered Locus Names:At3g45770
ORF Names:T6D9.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G45770.
TAIRilocus:2102664. AT3G45770.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37Mitochondrion1 PublicationAdd BLAST37
ChainiPRO_000000089538 – 375Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST338

Proteomic databases

PaxDbiQ8LCU7.

PTM databases

iPTMnetiQ8LCU7.

Expressioni

Gene expression databases

ExpressionAtlasiQ8LCU7. baseline and differential.
GenevisibleiQ8LCU7. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi9040. 2 interactors.
MINTiMINT-8069555.
STRINGi3702.AT3G45770.1.

Structurei

3D structure databases

ProteinModelPortaliQ8LCU7.
SMRiQ8LCU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0025. Eukaryota.
COG0604. LUCA.
HOGENOMiHOG000294683.
InParanoidiQ8LCU7.
KOiK07512.
OMAiAFRGFWM.
OrthoDBiEOG09360D49.
PhylomeDBiQ8LCU7.

Family and domain databases

InterProiView protein in InterPro
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q8LCU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALMESVVG RALKFSSTAN FRSIRRGETP TLCIKSFSTI MSPPSKAIVY
60 70 80 90 100
EEHGSPDSVT RLVNLPPVEV KENDVCVKMI AAPINPSDIN RIEGVYPVRP
110 120 130 140 150
PVPAVGGYEG VGEVYAVGSN VNGFSPGDWV IPSPPSSGTW QTYVVKEESV
160 170 180 190 200
WHKIDKECPM EYAATITVNP LTALRMLEDF VNLNSGDSVV QNGATSIVGQ
210 220 230 240 250
CVIQLARLRG ISTINLIRDR AGSDEAREQL KALGADEVFS ESQLNVKNVK
260 270 280 290 300
SLLGNLPEPA LGFNCVGGNA ASLVLKYLRE GGTMVTYGGM SKKPITVSTT
310 320 330 340 350
SFIFKDLALR GFWLQSWLSM GKVKECREMI DYLLGLARDG KLKYETELVP
360 370
FEEFPVALDK ALGKLGRQPK QVITF
Length:375
Mass (Da):40,823
Last modified:October 1, 2002 - v1
Checksum:i9047BCD122872F48
GO

Sequence cautioni

The sequence CAB75790 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293K → E in AAL32632 (PubMed:14593172).Curated1
Sequence conflicti293K → E in AAM47974 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157735 Genomic DNA. Translation: CAB75790.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78071.1.
AY062554 mRNA. Translation: AAL32632.1.
AY114655 mRNA. Translation: AAM47974.1.
AY086398 mRNA. Translation: AAM64465.1.
PIRiT47517.
RefSeqiNP_566881.1. NM_114446.4. [Q8LCU7-1]
UniGeneiAt.3719.

Genome annotation databases

EnsemblPlantsiAT3G45770.1; AT3G45770.1; AT3G45770. [Q8LCU7-1]
GeneIDi823720.
GrameneiAT3G45770.1; AT3G45770.1; AT3G45770.
KEGGiath:AT3G45770.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMECR_ARATH
AccessioniPrimary (citable) accession number: Q8LCU7
Secondary accession number(s): Q8W4H6, Q9M166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: August 30, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families