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Q8LCU7

- MECR_ARATH

UniProt

Q8LCU7 - MECR_ARATH

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Protein

Probable trans-2-enoyl-CoA reductase, mitochondrial

Gene

At3g45770

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis (By similarity).By similarity

Catalytic activityi

Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691NADPBy similarity
Binding sitei370 – 3701NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi195 – 1984NADPBy similarity
Nucleotide bindingi218 – 2203NADPBy similarity
Nucleotide bindingi287 – 2904NADPBy similarity
Nucleotide bindingi312 – 3143NADPBy similarity

GO - Molecular functioni

  1. ATP binding Source: TAIR
  2. copper ion binding Source: TAIR
  3. trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB-EC
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT3G45770-MONOMER.
ARA:GQT-1388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable trans-2-enoyl-CoA reductase, mitochondrial (EC:1.3.1.38)
Gene namesi
Ordered Locus Names:At3g45770
ORF Names:T6D9.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G45770.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. mitochondrion Source: TAIR
  3. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
BLAST
Chaini28 – 375348Probable trans-2-enoyl-CoA reductase, mitochondrialPRO_0000000895Add
BLAST

Proteomic databases

PaxDbiQ8LCU7.
PRIDEiQ8LCU7.

Expressioni

Gene expression databases

ExpressionAtlasiQ8LCU7. baseline and differential.
GenevestigatoriQ8LCU7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi9040. 1 interaction.
MINTiMINT-8069555.

Structurei

3D structure databases

ProteinModelPortaliQ8LCU7.
SMRiQ8LCU7. Positions 41-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0604.
HOGENOMiHOG000294683.
InParanoidiQ8LCU7.
KOiK07512.
OMAiCSTLWRV.
PhylomeDBiQ8LCU7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8LCU7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALMESVVG RALKFSSTAN FRSIRRGETP TLCIKSFSTI MSPPSKAIVY
60 70 80 90 100
EEHGSPDSVT RLVNLPPVEV KENDVCVKMI AAPINPSDIN RIEGVYPVRP
110 120 130 140 150
PVPAVGGYEG VGEVYAVGSN VNGFSPGDWV IPSPPSSGTW QTYVVKEESV
160 170 180 190 200
WHKIDKECPM EYAATITVNP LTALRMLEDF VNLNSGDSVV QNGATSIVGQ
210 220 230 240 250
CVIQLARLRG ISTINLIRDR AGSDEAREQL KALGADEVFS ESQLNVKNVK
260 270 280 290 300
SLLGNLPEPA LGFNCVGGNA ASLVLKYLRE GGTMVTYGGM SKKPITVSTT
310 320 330 340 350
SFIFKDLALR GFWLQSWLSM GKVKECREMI DYLLGLARDG KLKYETELVP
360 370
FEEFPVALDK ALGKLGRQPK QVITF
Length:375
Mass (Da):40,823
Last modified:October 1, 2002 - v1
Checksum:i9047BCD122872F48
GO

Sequence cautioni

The sequence CAB75790.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931K → E in AAL32632. (PubMed:14593172)Curated
Sequence conflicti293 – 2931K → E in AAM47974. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157735 Genomic DNA. Translation: CAB75790.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78071.1.
AY062554 mRNA. Translation: AAL32632.1.
AY114655 mRNA. Translation: AAM47974.1.
AY086398 mRNA. Translation: AAM64465.1.
PIRiT47517.
RefSeqiNP_566881.1. NM_114446.3. [Q8LCU7-1]
UniGeneiAt.3719.

Genome annotation databases

EnsemblPlantsiAT3G45770.1; AT3G45770.1; AT3G45770. [Q8LCU7-1]
GeneIDi823720.
KEGGiath:AT3G45770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157735 Genomic DNA. Translation: CAB75790.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE78071.1 .
AY062554 mRNA. Translation: AAL32632.1 .
AY114655 mRNA. Translation: AAM47974.1 .
AY086398 mRNA. Translation: AAM64465.1 .
PIRi T47517.
RefSeqi NP_566881.1. NM_114446.3. [Q8LCU7-1 ]
UniGenei At.3719.

3D structure databases

ProteinModelPortali Q8LCU7.
SMRi Q8LCU7. Positions 41-374.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 9040. 1 interaction.
MINTi MINT-8069555.

Proteomic databases

PaxDbi Q8LCU7.
PRIDEi Q8LCU7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G45770.1 ; AT3G45770.1 ; AT3G45770 . [Q8LCU7-1 ]
GeneIDi 823720.
KEGGi ath:AT3G45770.

Organism-specific databases

GeneFarmi 2939.
TAIRi AT3G45770.

Phylogenomic databases

eggNOGi COG0604.
HOGENOMi HOG000294683.
InParanoidi Q8LCU7.
KOi K07512.
OMAi CSTLWRV.
PhylomeDBi Q8LCU7.

Enzyme and pathway databases

BioCyci ARA:AT3G45770-MONOMER.
ARA:GQT-1388-MONOMER.

Miscellaneous databases

PROi Q8LCU7.

Gene expression databases

ExpressionAtlasi Q8LCU7. baseline and differential.
Genevestigatori Q8LCU7.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProi IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11695. PTHR11695. 1 hit.
Pfami PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50129. SSF50129. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiMECR_ARATH
AccessioniPrimary (citable) accession number: Q8LCU7
Secondary accession number(s): Q8W4H6, Q9M166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3