ID   GUN10_ARATH             Reviewed;         525 AA.
AC   Q8LCP6; Q9LR07;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=Endoglucanase 10;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 10;
DE   Flags: Precursor;
GN   OrderedLocusNames=At1g75680; ORFNames=F10A5.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene
RT   family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E)
CC       family.
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DR   EMBL; AC006434; AAF87112.1; -; Genomic_DNA.
DR   EMBL; AY039938; AAK64042.1; -; mRNA.
DR   EMBL; AY150451; AAN12892.1; -; mRNA.
DR   EMBL; AY086475; AAM63477.1; -; mRNA.
DR   IPI; IPI00516599; -.
DR   PIR; E96786; E96786.
DR   RefSeq; NP_177697.1; -.
DR   UniGene; At.23725; -.
DR   HSSP; P26221; 1TF4.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PRIDE; Q8LCP6; -.
DR   GeneID; 843902; -.
DR   GenomeReviews; CT485782_GR; AT1G75680.
DR   KEGG; ath:AT1G75680; -.
DR   NMPDR; fig|3702.1.peg.7086; -.
DR   TAIR; At1g75680; -.
DR   OMA; Q8LCP6; YDAGDHI.
DR   BRENDA; 3.2.1.4; 302.
DR   GermOnline; AT1G75680; Arabidopsis thaliana.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0008810; F:cellulase activity; IEA:EC.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR012341; 6hp_glycosidase.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_AS.
DR   Gene3D; G3DSA:1.50.10.10; CelA/Cel48F_cat; 1.
DR   PANTHER; PTHR22298:SF3; Glyco_hydro_9; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   PROSITE; PS00592; GLYCOSYL_HYDROL_F9_1; 1.
DR   PROSITE; PS00698; GLYCOSYL_HYDROL_F9_2; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Complete proteome; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL        1     26       Potential.
FT   CHAIN        27    525       Endoglucanase 10.
FT                                /FTId=PRO_0000249262.
FT   ACT_SITE    442    442       By similarity.
FT   ACT_SITE    489    489       By similarity.
FT   ACT_SITE    498    498       By similarity.
FT   CARBOHYD    259    259       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    464    464       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    484    484       N-linked (GlcNAc...) (Potential).
FT   CONFLICT     45     45       G -> C (in Ref. 3; AAM63477).
FT   CONFLICT    238    238       N -> D (in Ref. 3; AAM63477).
SQ   SEQUENCE   525 AA;  57868 MW;  FBC3704F4BB8BB71 CRC64;
     MEEKSKSRGW CGWFIAIIVL ASVILAVVYT VKLRTKKSGD DDGGGPVPGP PGAIDKKYAD
     ALKLALQFFD IQKSGKLENN KIPWRGDSGL KDGSEDNLDL SKGLYDAGDH IKFGFPMAFT
     ATVLSWSILE YGDQMNAVNQ LDPAKDSLRW ITDYLIKAHP SDNVLYIQVG DPKVDHPCWE
     RPEDMKEKRP LTKIDVDTPG TEVAAETAAA MASASLVFKD SDPTYSATLL KHAKQLFNFA
     DTKRGSYSVN IPEVQKFYNS TGYGDELLWA ASWLYHATED KTYLDYVSNH GKEFASFGNP
     TWFSWDNKLA GTQVLLSRLL FFKKDLSGSK GLGNYRNTAK AVMCGLLPKS PTSTASRTNG
     GLIWVSEWNS MQQSVSSAFL ASLFSDYMLT SRIHKISCDG KIFKATELRD FAKSQADYML
     GKNPLGTSFV VGYGDKYPQF VHHRGASIPA DATTGCLDGF KWFNSTKPNP NIAYGALVGG
     PFFNETFTDS RENPMQNEPT TYNNALLVGL LSSLVTTSST LQSLK
//