Reviewed, UniProtKB/Swiss-Prot Q8LCP6 (GUN10_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 10
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 10

Gene names

Ordered Locus Names:	At1g75680
ORF Names:	F10A5.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 525	499	Endoglucanase 10		PRO_0000249262
Sites
	Active site	442	1	By similarity
	Active site	489	1	By similarity
	Active site	498	1	By similarity
Amino acid modifications
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential
	Glycosylation	484	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	45	1	G → C in AAM63477. Ref.3
	Sequence conflict	238	1	N → D in AAM63477. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8LCP6-1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: FBC3704F4BB8BB71	FASTA	525	57,868
	10 20 30 40 50 60 MEEKSKSRGW CGWFIAIIVL ASVILAVVYT VKLRTKKSGD DDGGGPVPGP PGAIDKKYAD 70 80 90 100 110 120 ALKLALQFFD IQKSGKLENN KIPWRGDSGL KDGSEDNLDL SKGLYDAGDH IKFGFPMAFT 130 140 150 160 170 180 ATVLSWSILE YGDQMNAVNQ LDPAKDSLRW ITDYLIKAHP SDNVLYIQVG DPKVDHPCWE 190 200 210 220 230 240 RPEDMKEKRP LTKIDVDTPG TEVAAETAAA MASASLVFKD SDPTYSATLL KHAKQLFNFA 250 260 270 280 290 300 DTKRGSYSVN IPEVQKFYNS TGYGDELLWA ASWLYHATED KTYLDYVSNH GKEFASFGNP 310 320 330 340 350 360 TWFSWDNKLA GTQVLLSRLL FFKKDLSGSK GLGNYRNTAK AVMCGLLPKS PTSTASRTNG 370 380 390 400 410 420 GLIWVSEWNS MQQSVSSAFL ASLFSDYMLT SRIHKISCDG KIFKATELRD FAKSQADYML 430 440 450 460 470 480 GKNPLGTSFV VGYGDKYPQF VHHRGASIPA DATTGCLDGF KWFNSTKPNP NIAYGALVGG 490 500 510 520 PFFNETFTDS RENPMQNEPT TYNNALLVGL LSSLVTTSST LQSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006434 Genomic DNA. Translation: AAF87112.1. AY039938 mRNA. Translation: AAK64042.1. AY150451 mRNA. Translation: AAN12892.1. AY086475 mRNA. Translation: AAM63477.1.
IPI	IPI00516599.
PIR	E96786.
RefSeq	NP_177697.1.
UniGene	At.23725 Rsa.7375
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
SMR	Q8LCP6. Positions 57-517.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q8LCP6.
Genome annotation databases
GeneID	843902.
GenomeReviews	Gene locus AT1G75680 in contig CT485782_GR.
KEGG	ath:AT1G75680.
NMPDR	fig|3702.1.peg.7086.
Organism-specific databases
TAIR	At1g75680.
Phylogenomic databases
eggNOG	NOG26904.
HOGENOM	HBG746347.
InParanoid	Q8LCP6.
OMA	YDAGDHI.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
Genevestigator	Q8LCP6.
GermOnline	AT1G75680. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN10_ARATH

Accession

Primary (citable) accession number: Q8LCP6
Secondary accession number(s): Q9LR07

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)