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Q8LCE1

- GLN12_ARATH

UniProt

Q8LCE1 - GLN12_ARATH

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Protein

Glutamine synthetase cytosolic isozyme 1-2

Gene

GLN1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Low-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=3.8 mM for glutamate1 Publication
  2. KM=2450 µM for ammonium1 Publication
  3. KM=1100 µM for ATP1 Publication

Vmax=65.7 nmol/sec/mg enzyme with glutamate as substrate1 Publication

Vmax=65.7 nmol/sec/mg enzyme with ammonium as substrate1 Publication

Vmax=66.6 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-852-MONOMER.
ARA:GQT-853-MONOMER.
SABIO-RKQ8LCE1.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-2 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;2
Short name:
GLN1;2
Gene namesi
Name:GLN1-2
Synonyms:GSR2
Ordered Locus Names:At1g66200
ORF Names:F15E12.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G66200.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-2PRO_0000239819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8LCE1.
PRIDEiQ8LCE1.

Expressioni

Tissue specificityi

Expressed in the pericycle of all root tissues.1 Publication

Inductioni

By ammonium supply under nitrogen-limited condition. Induced by sucrose, glucose, fructose, and during leaf senescence.3 Publications

Gene expression databases

ExpressionAtlasiQ8LCE1. baseline and differential.
GenevestigatoriQ8LCE1.

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with GRF3.By similarity1 Publication

Protein-protein interaction databases

BioGridi28156. 1 interaction.
IntActiQ8LCE1. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8LCE1.
SMRiQ8LCE1. Positions 4-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ8LCE1.
PhylomeDBiQ8LCE1.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8LCE1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLADLVNL DISDNSEKII AEYIWVGGSG MDMRSKARTL PGPVTDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGQDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AEIFANPDVI AEVPWYGIEQ EYTLLQKDVN WPLGWPIGGF
160 170 180 190 200
PGPQGPYYCS IGADKSFGRD IVDAHYKASL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA ADEIWIARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTKSMREE GGYEIIKKAI EKLGLRHKEH ISAYGEGNER RLTGHHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET

TLLWNP
Length:356
Mass (Da):39,207
Last modified:June 13, 2006 - v2
Checksum:i19D19BF1388E5192
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti290 – 2901R → L in AAM63710. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC026480 Genomic DNA. Translation: AAG51310.1.
CP002684 Genomic DNA. Translation: AEE34474.1.
AY091101 mRNA. Translation: AAM14052.1.
AY122962 mRNA. Translation: AAM67495.1.
AY086653 mRNA. Translation: AAM63710.1.
PIRiH96686.
S18602.
RefSeqiNP_176794.1. NM_105291.3. [Q8LCE1-1]
UniGeneiAt.47484.
At.74857.

Genome annotation databases

EnsemblPlantsiAT1G66200.1; AT1G66200.1; AT1G66200. [Q8LCE1-1]
GeneIDi842935.
KEGGiath:AT1G66200.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC026480 Genomic DNA. Translation: AAG51310.1 .
CP002684 Genomic DNA. Translation: AEE34474.1 .
AY091101 mRNA. Translation: AAM14052.1 .
AY122962 mRNA. Translation: AAM67495.1 .
AY086653 mRNA. Translation: AAM63710.1 .
PIRi H96686.
S18602.
RefSeqi NP_176794.1. NM_105291.3. [Q8LCE1-1 ]
UniGenei At.47484.
At.74857.

3D structure databases

ProteinModelPortali Q8LCE1.
SMRi Q8LCE1. Positions 4-354.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 28156. 1 interaction.
IntActi Q8LCE1. 1 interaction.

Proteomic databases

PaxDbi Q8LCE1.
PRIDEi Q8LCE1.

Protocols and materials databases

DNASUi 842935.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G66200.1 ; AT1G66200.1 ; AT1G66200 . [Q8LCE1-1 ]
GeneIDi 842935.
KEGGi ath:AT1G66200.

Organism-specific databases

TAIRi AT1G66200.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q8LCE1.
PhylomeDBi Q8LCE1.

Enzyme and pathway databases

BioCyci ARA:GQT-852-MONOMER.
ARA:GQT-853-MONOMER.
SABIO-RK Q8LCE1.

Gene expression databases

ExpressionAtlasi Q8LCE1. baseline and differential.
Genevestigatori Q8LCE1.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis."
    Miller J.D., Arteca R.N., Pell E.J.
    Plant Physiol. 120:1015-1024(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
    Oliveira I.C., Coruzzi G.M.
    Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "14-3-3 proteins fine-tune plant nutrient metabolism."
    Shin R., Jez J.M., Basra A., Zhang B., Schachtman D.P.
    FEBS Lett. 585:143-147(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRF3.
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLN12_ARATH
AccessioniPrimary (citable) accession number: Q8LCE1
Secondary accession number(s): Q9C8C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3