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Q8LCE1

- GLN12_ARATH

UniProt

Q8LCE1 - GLN12_ARATH

Protein

Glutamine synthetase cytosolic isozyme 1-2

Gene

GLN1-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    Low-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=3.8 mM for glutamate1 Publication
    2. KM=2450 µM for ammonium1 Publication
    3. KM=1100 µM for ATP1 Publication

    Vmax=65.7 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=65.7 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=66.6 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-852-MONOMER.
    ARA:GQT-853-MONOMER.
    SABIO-RKQ8LCE1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;2
    Short name:
    GLN1;2
    Gene namesi
    Name:GLN1-2
    Synonyms:GSR2
    Ordered Locus Names:At1g66200
    ORF Names:F15E12.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G66200.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-2PRO_0000239819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21Phosphoserine1 Publication
    Modified residuei48 – 481PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8LCE1.
    PRIDEiQ8LCE1.

    Expressioni

    Tissue specificityi

    Expressed in the pericycle of all root tissues.1 Publication

    Inductioni

    By ammonium supply under nitrogen-limited condition. Induced by sucrose, glucose, fructose, and during leaf senescence.3 Publications

    Gene expression databases

    ArrayExpressiQ8LCE1.
    GenevestigatoriQ8LCE1.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi28156. 1 interaction.
    IntActiQ8LCE1. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8LCE1.
    SMRiQ8LCE1. Positions 4-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ8LCE1.
    PhylomeDBiQ8LCE1.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q8LCE1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MSLLADLVNL DISDNSEKII AEYIWVGGSG MDMRSKARTL PGPVTDPSKL    50
    PKWNYDGSST GQAPGQDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE 100
    PIPTNKRHAA AEIFANPDVI AEVPWYGIEQ EYTLLQKDVN WPLGWPIGGF 150
    PGPQGPYYCS IGADKSFGRD IVDAHYKASL YAGINISGIN GEVMPGQWEF 200
    QVGPSVGISA ADEIWIARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT 250
    NYSTKSMREE GGYEIIKKAI EKLGLRHKEH ISAYGEGNER RLTGHHETAD 300
    INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET 350
    TLLWNP 356
    Length:356
    Mass (Da):39,207
    Last modified:June 13, 2006 - v2
    Checksum:i19D19BF1388E5192
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti290 – 2901R → L in AAM63710. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC026480 Genomic DNA. Translation: AAG51310.1.
    CP002684 Genomic DNA. Translation: AEE34474.1.
    AY091101 mRNA. Translation: AAM14052.1.
    AY122962 mRNA. Translation: AAM67495.1.
    AY086653 mRNA. Translation: AAM63710.1.
    PIRiH96686.
    S18602.
    RefSeqiNP_176794.1. NM_105291.3. [Q8LCE1-1]
    UniGeneiAt.47484.
    At.74857.

    Genome annotation databases

    EnsemblPlantsiAT1G66200.1; AT1G66200.1; AT1G66200. [Q8LCE1-1]
    GeneIDi842935.
    KEGGiath:AT1G66200.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC026480 Genomic DNA. Translation: AAG51310.1 .
    CP002684 Genomic DNA. Translation: AEE34474.1 .
    AY091101 mRNA. Translation: AAM14052.1 .
    AY122962 mRNA. Translation: AAM67495.1 .
    AY086653 mRNA. Translation: AAM63710.1 .
    PIRi H96686.
    S18602.
    RefSeqi NP_176794.1. NM_105291.3. [Q8LCE1-1 ]
    UniGenei At.47484.
    At.74857.

    3D structure databases

    ProteinModelPortali Q8LCE1.
    SMRi Q8LCE1. Positions 4-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 28156. 1 interaction.
    IntActi Q8LCE1. 1 interaction.

    Proteomic databases

    PaxDbi Q8LCE1.
    PRIDEi Q8LCE1.

    Protocols and materials databases

    DNASUi 842935.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G66200.1 ; AT1G66200.1 ; AT1G66200 . [Q8LCE1-1 ]
    GeneIDi 842935.
    KEGGi ath:AT1G66200.

    Organism-specific databases

    TAIRi AT1G66200.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q8LCE1.
    PhylomeDBi Q8LCE1.

    Enzyme and pathway databases

    BioCyci ARA:GQT-852-MONOMER.
    ARA:GQT-853-MONOMER.
    SABIO-RK Q8LCE1.

    Gene expression databases

    ArrayExpressi Q8LCE1.
    Genevestigatori Q8LCE1.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis."
      Miller J.D., Arteca R.N., Pell E.J.
      Plant Physiol. 120:1015-1024(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
      Oliveira I.C., Coruzzi G.M.
      Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
      Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
      J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLN12_ARATH
    AccessioniPrimary (citable) accession number: Q8LCE1
    Secondary accession number(s): Q9C8C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3